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The map label for this gene is cobS [H]

Identifier: 238028357

GI number: 238028357

Start: 3197519

End: 3198286

Strand: Reverse

Name: cobS [H]

Synonym: bglu_1g28180

Alternate gene names: 238028357

Gene position: 3198286-3197519 (Counterclockwise)

Preceding gene: 238028358

Following gene: 238028356

Centisome position: 81.87

GC content: 75.39

Gene sequence:

>768_bases
ATGCGCTGGTCCGCCGAATTGCGCTATTTCTTCGTCGCGCTCGGCTATTTCACGCGCGTGCCGATCCCGCGGCGGATCGG
CTACGCGGCGGGCGACCTCGATCAAGCGGCGCGCTACTTCCCGCTGGTCGGCGTCGGCGTCGGCGCGCTCGCGGCGGCCG
TCTACCTGGGCGCGGCGCGGATCTGGCCGGCCGGCGTGGCGGTGCTGCTGTCGATGGCCGCCACGCTGGTCGCGACCGGC
GCGTTCCACGAGGACGGCCTGGCCGATAGCTGCGACGGCTTCGGCGGCGGCTACGCGCGCGACGAGGTGCTGCGGATCAT
GCGCGACTCGCGGATCGGCACGTTCGGCGCGGCCGCGCTCGCCGTCTCGCTGGCGCTGAAGTGGCAGGCGCTCGCGGCGC
TGCCGCCCGCGCGCGCGGCCTGGACCCTGCTGGCCGCGCATGCGGCGAGCCGGGTCGGCGCGGTGAGCCTGCTCGTCACG
CTCGACTACGTGCGCGAGGAGGGCAAGGCCAGGCCGGTCGCGCGGCGCATGTCGGCGCGCGCGTTCGCGTTCGCGGCGCT
GTGCGGGCTGCCGTGGCTGCTCTGCCCGGGCTGGCCGGACTGGCGCCTGGGGCTCGCCGCGCTGGCCGCGCTGCTGGCGG
CGCGGGCCGGCATCGCGCGTTATCTGCGGCGCCGGCTCGGCGGATACACGGGCGACTGCCTCGGCTTCGCGCAGCAGCTG
TGCGAGCTCGCGATCTACCTGACGGTGCTCGGATGGACGTCGTCCTGA

Upstream 100 bases:

>100_bases
CTGCCGCTGCTGCGCGCGGCGGCCGCGTTCGTCAACGAGATGGCCAGCTTCGAGGCCGCCGGCGTCGCGAACCGCGGCGA
CGCGTAGGCGGGGGCACGCG

Downstream 100 bases:

>100_bases
TCCGGCATCCGGCGCCGGCCGTTGCCGGGGGCGTCTGCTACGGCGCGACCGATCTGCCGCTCGCGGGCGACGCGGCGGCG
GCGGCGGCCGAACTGGCGGG

Product: cobalamin synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MRWSAELRYFFVALGYFTRVPIPRRIGYAAGDLDQAARYFPLVGVGVGALAAAVYLGAARIWPAGVAVLLSMAATLVATG
AFHEDGLADSCDGFGGGYARDEVLRIMRDSRIGTFGAAALAVSLALKWQALAALPPARAAWTLLAAHAASRVGAVSLLVT
LDYVREEGKARPVARRMSARAFAFAALCGLPWLLCPGWPDWRLGLAALAALLAARAGIARYLRRRLGGYTGDCLGFAQQL
CELAIYLTVLGWTSS

Sequences:

>Translated_255_residues
MRWSAELRYFFVALGYFTRVPIPRRIGYAAGDLDQAARYFPLVGVGVGALAAAVYLGAARIWPAGVAVLLSMAATLVATG
AFHEDGLADSCDGFGGGYARDEVLRIMRDSRIGTFGAAALAVSLALKWQALAALPPARAAWTLLAAHAASRVGAVSLLVT
LDYVREEGKARPVARRMSARAFAFAALCGLPWLLCPGWPDWRLGLAALAALLAARAGIARYLRRRLGGYTGDCLGFAQQL
CELAIYLTVLGWTSS
>Mature_255_residues
MRWSAELRYFFVALGYFTRVPIPRRIGYAAGDLDQAARYFPLVGVGVGALAAAVYLGAARIWPAGVAVLLSMAATLVATG
AFHEDGLADSCDGFGGGYARDEVLRIMRDSRIGTFGAAALAVSLALKWQALAALPPARAAWTLLAAHAASRVGAVSLLVT
LDYVREEGKARPVARRMSARAFAFAALCGLPWLLCPGWPDWRLGLAALAALLAARAGIARYLRRRLGGYTGDCLGFAQQL
CELAIYLTVLGWTSS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=253, Percent_Identity=35.9683794466403, Blast_Score=114, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 27093; Mature: 27093

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWSAELRYFFVALGYFTRVPIPRRIGYAAGDLDQAARYFPLVGVGVGALAAAVYLGAAR
CCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IWPAGVAVLLSMAATLVATGAFHEDGLADSCDGFGGGYARDEVLRIMRDSRIGTFGAAAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
AVSLALKWQALAALPPARAAWTLLAAHAASRVGAVSLLVTLDYVREEGKARPVARRMSAR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AFAFAALCGLPWLLCPGWPDWRLGLAALAALLAARAGIARYLRRRLGGYTGDCLGFAQQL
HHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
CELAIYLTVLGWTSS
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MRWSAELRYFFVALGYFTRVPIPRRIGYAAGDLDQAARYFPLVGVGVGALAAAVYLGAAR
CCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IWPAGVAVLLSMAATLVATGAFHEDGLADSCDGFGGGYARDEVLRIMRDSRIGTFGAAAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
AVSLALKWQALAALPPARAAWTLLAAHAASRVGAVSLLVTLDYVREEGKARPVARRMSAR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AFAFAALCGLPWLLCPGWPDWRLGLAALAALLAARAGIARYLRRRLGGYTGDCLGFAQQL
HHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
CELAIYLTVLGWTSS
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA