Click here to switch to the map view.

The map label for this gene is hemX [H]

Identifier: 238028331

GI number: 238028331

Start: 3162489

End: 3164762

Strand: Reverse

Name: hemX [H]

Synonym: bglu_1g27920

Alternate gene names: 238028331

Gene position: 3164762-3162489 (Counterclockwise)

Preceding gene: 238028332

Following gene: 238028330

Centisome position: 81.01

GC content: 73.7

Gene sequence:

>2274_bases
ATGGCCGCCGCCCGCGCGTTCACGGCGGTGCTGACCAAGCCCGAGGGGCAATCGGCGGCGCTCGCCGCGCAGCTCGCGGC
GGCCGGCATCGAAAGCTTCGATTTTCCGCTGATCGACATCGCGCCGGCGACGGACTTCGGCCCGCTCGACGCGGCGTTCG
CCCGCCTCGACGCCTATGCGCTGGTGGTGTTCGTCTCGCCGAACGCGATCGACCGCGCGCTCGCGCGCTTCGGCGCGATC
TGGCCGCATTCGCTGCCGGTGGGCGTGGTCGGTCCCGGCAGCGTGGCGGCGCTGGCGCGCCACGGCATCGCGGCACCCGC
GCATCGCGTGATCGCACCGGACGGCGCGCTGGCGCCCGCCGGCGCCGCCGGGACCGACGGCGGCGAGCCGCGCTACGATT
CCGAAGGCCTTTACGCGCAGATCGAACGCGCGTTCGGCATGGCGGCTGCGCACCCGGCGGCCGGGCAGGTCGCCGATGAC
GCGGCCGGCGCCACGGCGAGCGGCGGCGTGGCCCTGGACCGTCGTGACGACACTGCCGCCCGCGCAGCCGGCGCCGGCCC
GGACGCCGTGCCGGACGCTCCGGGTGGCTCGGACGGCTCCGGTTCCCCCGCGGCATCGGCGGCGGCGGCTCGCGAGTCCG
CCGCCGCGGCGCATGCGCAGGCCGGGCGTGCCGATGCCGCCATGCCGCCGCCCGCCGGACCGCTTGCCGGCAAGCGCGTG
CTGATCGTGCGCGGCGACGGCGGCCGCGAATGGCTCGCCACGCGGCTGCGCGAGGCCGGCGCCGAGGTTGAGTTGGTGGC
CGCCTATCGCCGGATCGTGCCCGAGCCTTCGATCGGTGCCTGGGAGCGCGTCCATGCGCTGCTCGGCGGCGCGCCGCACG
CGTGGCTCGTGACGAGTTCGGAGGGCGTGCGCAATCTGCACGAGCTGGCGGTCGACCATCTGACCGCAGCCGAGATCGAG
GCGCTGCTGCACGCGCCGCTGGTCGCGCCCCATTCGCGCATTGCCGATACGGCGCGGGCATTGGGTTTTGATAGGATTAC
GCAGTCCGGCGCGGGTGACGAACGGATCGTCCGCGCGTTCAGAACGTTGGCCGACACGGCCGCTCACGCGGTGCAAGCCG
CCTCGGTACCCTCTCGCATGACAGACACTAACGATTCCAGCAAGGCTTCCTCCGCGGGCGGCGCGCCCGCGCGGCCCGCG
GCGCCGCCGCCGCCCAATCCGCCGTTTACGCCGTTCGAGGCGCAAGGCCCGAAACGCCGCGGCGGCGCGGCCGGCGTGCT
GCTCTGGTGCGTGGTGGTGGTGCTCGCCTGCGGCGCGGGCGCCGGCGGCTACGTGCTGAACCGCAAGCTCGACAAGCTCG
AGCAGCTCACGGTGCAGCGCCAGAACGCGCTCGACGCGGCCACCGCGGCGGCGCGGCGCCAGTCCGAGCAGGCGCTCGCC
AACAGTCATCAGGTGGACAGCGAGGTCTCGCAGTTCGCCGGCAAGCTCGCCGACGCGCAGGCCCAGCAGCAGGCGCTGCA
GCAGCAGTACCAGGACCTGTCGCGCAATCGCGACGCCTGGCTGCTCGACCAGGTCGAGCAGACGCTGTCGGGCGCGAGCC
AGCAGCTGCAGCTCACGGGCGATACGCAGCTCGCGCTGGTCGCGCTGCAGAACGCCGACGCGCAGCTCGCCACCTCGCAG
GGCGCGCAGGCGATCGCGGTGCGCAAGGCGATCGCGCAGGACATCGACAAGCTCAAGGCCGCGCCGTCCGTGGACCTGAC
CGGCCTCGCCATCAAGCTCGACGACGCGATCGGCCGCATCGACGCGCTGCCGCTTGCCGGCGAGGCGTCGATCGGCCGCG
CGGTGCCGCGCGCCGGGCATCCGGCCGACCTCGCGCAGCAGGCCGCCGCCACCGGCCAGCCACGATGGAAGATCTGGTGG
CACGACCTGTCCACCGGCGTCGCCGCGCAGCTCGCGAGCCTCGTGCAGGTGCGCCGCATCGACCACGCCGACGCGATGCT
GATCTCGCCCGAGCAGGGCTACTTCGTGCGCGAGAACGTCAAGCTGCGCCTGCTGACGGCGCGGCTCTCGCTGCTCGCGC
GCAACGAAGCGGCGATGAAGTCCGACCTGCACGCGGCGCAGGCCGCGCTCGGCCGCTATTTCGACGCCTCGTCGAAGGAC
ACGCGCACCGTGCAGGACCTGCTCAAGCAGGTCGACGCGGCCTCGCTGTCGGTGGCGGTGCCGAACCTGAACACGAGCCT
CTCCGCGATCCAACAGTTCAAGAGCCGGGGGTGA

Upstream 100 bases:

>100_bases
CGCCCGACGCGGCGCTCGCGCTGGGCCGGCAGGTCGCCGACGAGCTGGCGCGGCAAGGTGCCTTCGAGATCGTCGAGGCA
CTGCTCGGAGGGCGCTCCTG

Downstream 100 bases:

>100_bases
CGATGACGCTGCGAGGAATCCTCTGGCTGGCCGTGCTGTTCGCCGTCGCCGTGGCGCTCGCCACGGTCGGCCGCTTCGAC
GGCGGCCAGGTGCTGATCGT

Product: bifunctional uroporphyrinogen-III synthetase/uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen-III methylase; ORF X [H]

Number of amino acids: Translated: 757; Mature: 756

Protein sequence:

>757_residues
MAAARAFTAVLTKPEGQSAALAAQLAAAGIESFDFPLIDIAPATDFGPLDAAFARLDAYALVVFVSPNAIDRALARFGAI
WPHSLPVGVVGPGSVAALARHGIAAPAHRVIAPDGALAPAGAAGTDGGEPRYDSEGLYAQIERAFGMAAAHPAAGQVADD
AAGATASGGVALDRRDDTAARAAGAGPDAVPDAPGGSDGSGSPAASAAAARESAAAAHAQAGRADAAMPPPAGPLAGKRV
LIVRGDGGREWLATRLREAGAEVELVAAYRRIVPEPSIGAWERVHALLGGAPHAWLVTSSEGVRNLHELAVDHLTAAEIE
ALLHAPLVAPHSRIADTARALGFDRITQSGAGDERIVRAFRTLADTAAHAVQAASVPSRMTDTNDSSKASSAGGAPARPA
APPPPNPPFTPFEAQGPKRRGGAAGVLLWCVVVVLACGAGAGGYVLNRKLDKLEQLTVQRQNALDAATAAARRQSEQALA
NSHQVDSEVSQFAGKLADAQAQQQALQQQYQDLSRNRDAWLLDQVEQTLSGASQQLQLTGDTQLALVALQNADAQLATSQ
GAQAIAVRKAIAQDIDKLKAAPSVDLTGLAIKLDDAIGRIDALPLAGEASIGRAVPRAGHPADLAQQAAATGQPRWKIWW
HDLSTGVAAQLASLVQVRRIDHADAMLISPEQGYFVRENVKLRLLTARLSLLARNEAAMKSDLHAAQAALGRYFDASSKD
TRTVQDLLKQVDAASLSVAVPNLNTSLSAIQQFKSRG

Sequences:

>Translated_757_residues
MAAARAFTAVLTKPEGQSAALAAQLAAAGIESFDFPLIDIAPATDFGPLDAAFARLDAYALVVFVSPNAIDRALARFGAI
WPHSLPVGVVGPGSVAALARHGIAAPAHRVIAPDGALAPAGAAGTDGGEPRYDSEGLYAQIERAFGMAAAHPAAGQVADD
AAGATASGGVALDRRDDTAARAAGAGPDAVPDAPGGSDGSGSPAASAAAARESAAAAHAQAGRADAAMPPPAGPLAGKRV
LIVRGDGGREWLATRLREAGAEVELVAAYRRIVPEPSIGAWERVHALLGGAPHAWLVTSSEGVRNLHELAVDHLTAAEIE
ALLHAPLVAPHSRIADTARALGFDRITQSGAGDERIVRAFRTLADTAAHAVQAASVPSRMTDTNDSSKASSAGGAPARPA
APPPPNPPFTPFEAQGPKRRGGAAGVLLWCVVVVLACGAGAGGYVLNRKLDKLEQLTVQRQNALDAATAAARRQSEQALA
NSHQVDSEVSQFAGKLADAQAQQQALQQQYQDLSRNRDAWLLDQVEQTLSGASQQLQLTGDTQLALVALQNADAQLATSQ
GAQAIAVRKAIAQDIDKLKAAPSVDLTGLAIKLDDAIGRIDALPLAGEASIGRAVPRAGHPADLAQQAAATGQPRWKIWW
HDLSTGVAAQLASLVQVRRIDHADAMLISPEQGYFVRENVKLRLLTARLSLLARNEAAMKSDLHAAQAALGRYFDASSKD
TRTVQDLLKQVDAASLSVAVPNLNTSLSAIQQFKSRG
>Mature_756_residues
AAARAFTAVLTKPEGQSAALAAQLAAAGIESFDFPLIDIAPATDFGPLDAAFARLDAYALVVFVSPNAIDRALARFGAIW
PHSLPVGVVGPGSVAALARHGIAAPAHRVIAPDGALAPAGAAGTDGGEPRYDSEGLYAQIERAFGMAAAHPAAGQVADDA
AGATASGGVALDRRDDTAARAAGAGPDAVPDAPGGSDGSGSPAASAAAARESAAAAHAQAGRADAAMPPPAGPLAGKRVL
IVRGDGGREWLATRLREAGAEVELVAAYRRIVPEPSIGAWERVHALLGGAPHAWLVTSSEGVRNLHELAVDHLTAAEIEA
LLHAPLVAPHSRIADTARALGFDRITQSGAGDERIVRAFRTLADTAAHAVQAASVPSRMTDTNDSSKASSAGGAPARPAA
PPPPNPPFTPFEAQGPKRRGGAAGVLLWCVVVVLACGAGAGGYVLNRKLDKLEQLTVQRQNALDAATAAARRQSEQALAN
SHQVDSEVSQFAGKLADAQAQQQALQQQYQDLSRNRDAWLLDQVEQTLSGASQQLQLTGDTQLALVALQNADAQLATSQG
AQAIAVRKAIAQDIDKLKAAPSVDLTGLAIKLDDAIGRIDALPLAGEASIGRAVPRAGHPADLAQQAAATGQPRWKIWWH
DLSTGVAAQLASLVQVRRIDHADAMLISPEQGYFVRENVKLRLLTARLSLLARNEAAMKSDLHAAQAALGRYFDASSKDT
RTVQDLLKQVDAASLSVAVPNLNTSLSAIQQFKSRG

Specific function: Siroheme and cobalamin biosynthesis. [C]

COG id: COG2959

COG function: function code H; Uncharacterized enzyme of heme biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790235, Length=261, Percent_Identity=26.4367816091954, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007470 [H]

Pfam domain/function: PF04375 HemX [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 78003; Mature: 77872

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAARAFTAVLTKPEGQSAALAAQLAAAGIESFDFPLIDIAPATDFGPLDAAFARLDAYA
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCEEE
LVVFVSPNAIDRALARFGAIWPHSLPVGVVGPGSVAALARHGIAAPAHRVIAPDGALAPA
EEEEECCCHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHCCCCCCCCEEECCCCCCCCC
GAAGTDGGEPRYDSEGLYAQIERAFGMAAAHPAAGQVADDAAGATASGGVALDRRDDTAA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEECCCCHHH
RAAGAGPDAVPDAPGGSDGSGSPAASAAAARESAAAAHAQAGRADAAMPPPAGPLAGKRV
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
LIVRGDGGREWLATRLREAGAEVELVAAYRRIVPEPSIGAWERVHALLGGAPHAWLVTSS
EEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECC
EGVRNLHELAVDHLTAAEIEALLHAPLVAPHSRIADTARALGFDRITQSGAGDERIVRAF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RTLADTAAHAVQAASVPSRMTDTNDSSKASSAGGAPARPAAPPPPNPPFTPFEAQGPKRR
HHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGAAGVLLWCVVVVLACGAGAGGYVLNRKLDKLEQLTVQRQNALDAATAAARRQSEQALA
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSHQVDSEVSQFAGKLADAQAQQQALQQQYQDLSRNRDAWLLDQVEQTLSGASQQLQLTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECC
DTQLALVALQNADAQLATSQGAQAIAVRKAIAQDIDKLKAAPSVDLTGLAIKLDDAIGRI
CCEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHCCH
DALPLAGEASIGRAVPRAGHPADLAQQAAATGQPRWKIWWHDLSTGVAAQLASLVQVRRI
HCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHC
DHADAMLISPEQGYFVRENVKLRLLTARLSLLARNEAAMKSDLHAAQAALGRYFDASSKD
CCCCEEEECCCCCEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TRTVQDLLKQVDAASLSVAVPNLNTSLSAIQQFKSRG
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AAARAFTAVLTKPEGQSAALAAQLAAAGIESFDFPLIDIAPATDFGPLDAAFARLDAYA
CCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCEEE
LVVFVSPNAIDRALARFGAIWPHSLPVGVVGPGSVAALARHGIAAPAHRVIAPDGALAPA
EEEEECCCHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHCCCCCCCCEEECCCCCCCCC
GAAGTDGGEPRYDSEGLYAQIERAFGMAAAHPAAGQVADDAAGATASGGVALDRRDDTAA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEECCCCHHH
RAAGAGPDAVPDAPGGSDGSGSPAASAAAARESAAAAHAQAGRADAAMPPPAGPLAGKRV
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
LIVRGDGGREWLATRLREAGAEVELVAAYRRIVPEPSIGAWERVHALLGGAPHAWLVTSS
EEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECC
EGVRNLHELAVDHLTAAEIEALLHAPLVAPHSRIADTARALGFDRITQSGAGDERIVRAF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RTLADTAAHAVQAASVPSRMTDTNDSSKASSAGGAPARPAAPPPPNPPFTPFEAQGPKRR
HHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGAAGVLLWCVVVVLACGAGAGGYVLNRKLDKLEQLTVQRQNALDAATAAARRQSEQALA
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSHQVDSEVSQFAGKLADAQAQQQALQQQYQDLSRNRDAWLLDQVEQTLSGASQQLQLTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECC
DTQLALVALQNADAQLATSQGAQAIAVRKAIAQDIDKLKAAPSVDLTGLAIKLDDAIGRI
CCEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHCCH
DALPLAGEASIGRAVPRAGHPADLAQQAAATGQPRWKIWWHDLSTGVAAQLASLVQVRRI
HCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHC
DHADAMLISPEQGYFVRENVKLRLLTARLSLLARNEAAMKSDLHAAQAALGRYFDASSKD
CCCCEEEECCCCCEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TRTVQDLLKQVDAASLSVAVPNLNTSLSAIQQFKSRG
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3062586; 3054815; 1379743; 9278503; 9298646 [H]