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The map label for this gene is argT [H]

Identifier: 238028316

GI number: 238028316

Start: 3146435

End: 3147211

Strand: Direct

Name: argT [H]

Synonym: bglu_1g27770

Alternate gene names: 238028316

Gene position: 3146435-3147211 (Clockwise)

Preceding gene: 238028315

Following gene: 238028317

Centisome position: 80.54

GC content: 66.02

Gene sequence:

>777_bases
ATGAAGAAGCTCGCCCTGTGCGCAGCACTCGCCATGGCGGCGAGCGGCGCCGTCGCGAAGGAATGGAAGACGGTCCGGAT
CGGCGTCGACGCCAGTTACCCGCCGTTCGAATCGGTCGCGCAGAACGGCGACATCGTCGGCTTCGACGTCGACCTGGCGA
AGGAAATCTGCACGCGCATCGCGGTGCGCTGCACCTGGGTCGCGCAGGATCTCGACGGGATCATCCCGGCCCTGAAGGCG
CGCAAGTTCGACGTGATCATGTCGTCGCTGACCGTCACCGACAAGCGCCGCGAGCAGATCGACTTCTCGGACAAGCTGTA
CGACGCGCCCGCGCGGATGATCGCGCCGGCCGGCTCGCCGCTGCTGCCCACGCTCGCCTCGCTCAAGGGCAAGCGCGTCG
GGGTCGAGCAGGGGTCGACGCAGGAAACCTACGCGAAGACCTACTGGGAGCCGCGCGGTGTGACGATCGTGCCGTACCAA
AACCAGGATCAGGTCTACGCCGATCTCGGGACCGGCCGGCTCGACGCCGCGCTGCAGGACGAATTGCAGGCCGACTACGG
GTTCCTGCGCACGCCGCGCGGCAAGGGTTTCGCGTGGGCGGGCCCGGAAGTGAAGGACCCGAAGACGATCGGCGACGGCA
CCGCGATCGGCCTGCGCAAGGAGGACACGGATCTGAAGGCGAAGATCAACGCCGCTCTCGCCGCGATGCACAAGGATGGG
ACTTACGACAGGTTGTCGCACAAGTACTTCTCGTTCAGCGTGTATTCGGCGAAGTAA

Upstream 100 bases:

>100_bases
CCCGATCGCCGAAGCCGCCGACACCGAATTTTGACTGACTAGAATACGGCCGGCGCGGCGCGGGCGAAGGCCGGCGCGCG
GCAGTCGTAAGAGGAATCAG

Downstream 100 bases:

>100_bases
CCACGTTTCGGCCGGACTGGCCGCCGCGCGTCACCGCGCGCGGCGGCCAGTCCGGCACGAGGAAGTCGCCAGCCGCCCGG
CGGAATCACCGGGCAGCCCG

Product: Cationic amino acid ABC transporter periplasmic binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKKLALCAALAMAASGAVAKEWKTVRIGVDASYPPFESVAQNGDIVGFDVDLAKEICTRIAVRCTWVAQDLDGIIPALKA
RKFDVIMSSLTVTDKRREQIDFSDKLYDAPARMIAPAGSPLLPTLASLKGKRVGVEQGSTQETYAKTYWEPRGVTIVPYQ
NQDQVYADLGTGRLDAALQDELQADYGFLRTPRGKGFAWAGPEVKDPKTIGDGTAIGLRKEDTDLKAKINAALAAMHKDG
TYDRLSHKYFSFSVYSAK

Sequences:

>Translated_258_residues
MKKLALCAALAMAASGAVAKEWKTVRIGVDASYPPFESVAQNGDIVGFDVDLAKEICTRIAVRCTWVAQDLDGIIPALKA
RKFDVIMSSLTVTDKRREQIDFSDKLYDAPARMIAPAGSPLLPTLASLKGKRVGVEQGSTQETYAKTYWEPRGVTIVPYQ
NQDQVYADLGTGRLDAALQDELQADYGFLRTPRGKGFAWAGPEVKDPKTIGDGTAIGLRKEDTDLKAKINAALAAMHKDG
TYDRLSHKYFSFSVYSAK
>Mature_258_residues
MKKLALCAALAMAASGAVAKEWKTVRIGVDASYPPFESVAQNGDIVGFDVDLAKEICTRIAVRCTWVAQDLDGIIPALKA
RKFDVIMSSLTVTDKRREQIDFSDKLYDAPARMIAPAGSPLLPTLASLKGKRVGVEQGSTQETYAKTYWEPRGVTIVPYQ
NQDQVYADLGTGRLDAALQDELQADYGFLRTPRGKGFAWAGPEVKDPKTIGDGTAIGLRKEDTDLKAKINAALAAMHKDG
TYDRLSHKYFSFSVYSAK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=259, Percent_Identity=57.5289575289575, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=54.0772532188841, Blast_Score=281, Evalue=3e-77,
Organism=Escherichia coli, GI1787085, Length=251, Percent_Identity=38.2470119521912, Blast_Score=173, Evalue=9e-45,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=36, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=31.7269076305221, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1788228, Length=239, Percent_Identity=30.1255230125523, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28142; Mature: 28142

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLALCAALAMAASGAVAKEWKTVRIGVDASYPPFESVAQNGDIVGFDVDLAKEICTRI
CCHHHHHHHHHHHHCCCHHHCCEEEEEECCCCCCCHHHHHCCCCEEEECHHHHHHHHHHH
AVRCTWVAQDLDGIIPALKARKFDVIMSSLTVTDKRREQIDFSDKLYDAPARMIAPAGSP
HHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHCCCHHHCCCCCCC
LLPTLASLKGKRVGVEQGSTQETYAKTYWEPRGVTIVPYQNQDQVYADLGTGRLDAALQD
HHHHHHHCCCCEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHH
ELQADYGFLRTPRGKGFAWAGPEVKDPKTIGDGTAIGLRKEDTDLKAKINAALAAMHKDG
HHHHCCCEEECCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
TYDRLSHKYFSFSVYSAK
CHHHHCCEEEEEEEEECC
>Mature Secondary Structure
MKKLALCAALAMAASGAVAKEWKTVRIGVDASYPPFESVAQNGDIVGFDVDLAKEICTRI
CCHHHHHHHHHHHHCCCHHHCCEEEEEECCCCCCCHHHHHCCCCEEEECHHHHHHHHHHH
AVRCTWVAQDLDGIIPALKARKFDVIMSSLTVTDKRREQIDFSDKLYDAPARMIAPAGSP
HHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHCCCHHHCCCCCCC
LLPTLASLKGKRVGVEQGSTQETYAKTYWEPRGVTIVPYQNQDQVYADLGTGRLDAALQD
HHHHHHHCCCCEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHH
ELQADYGFLRTPRGKGFAWAGPEVKDPKTIGDGTAIGLRKEDTDLKAKINAALAAMHKDG
HHHHCCCEEECCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
TYDRLSHKYFSFSVYSAK
CHHHHCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]