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The map label for this gene is dnaB [H]

Identifier: 238027635

GI number: 238027635

Start: 2247312

End: 2248706

Strand: Reverse

Name: dnaB [H]

Synonym: bglu_1g20560

Alternate gene names: 238027635

Gene position: 2248706-2247312 (Counterclockwise)

Preceding gene: 238027636

Following gene: 238027634

Centisome position: 57.56

GC content: 63.23

Gene sequence:

>1395_bases
ATGAACGCTGTCGAAAACTATTTCGGCGACGGGGCGATCGATGTGCCGCACAGCATCGAGGCCGAGCAATCGGTCCTCGG
TGCGCTGATGATCGACAACGACTCGATCGACCGCATTGGCGCCCTGCAACAGGAACACTTCTTCCGCTACGAGCACCGGA
TCATCTTCGAGGGCGCCACCAAGCTGATTGCCGTCGGCCGTTCCGCTGACGCCGTGACGGTCTACGAATGGCTGCAAGCG
CATGGCCATGTCGAGAAGACCGGCGGCCTGCCTTACCTCAACTCGATTGTTCAGAGCACGCCCGGCTCCGCCAACATGGC
GCGCTATGCCGAGATCGTTGTGGAGCGCGCGAAGCTGCGGTACATCATGGCGGCCGCAGACAAAGTGAGCGCCGAGGTTC
AAAACCGGAACGGTCGGACGGCTGACGAGCTGACAGCCTTTGCGCAATCGCAGTTCGAGCCACTTTCGGAAGGGCGCACA
GACGGGCCGAAATTCATCGGCACCTTCCTGAGCCCCGTGGTCACGCAGATGGATCAGGAGCATCACGGGAACGCGCCGCT
CGCTACGGCGACGGGTTACCGTGAGCTCGACTTCAAGCTGGGCGGCGGCGTGCGCGGTGGTGATCTGATCGTCGTCGCTG
CGCGGCCTTCGATGGGTAAGACCGCGATGGCGCAGGGCATTGCCGAATACGTTGCGCAGACCCAGGGCACCGCGCTGATC
TTCTCATTGGAGATGCCTGGCACGCAGCTTACGCAGCGCGCGATCGCGCGGCAGGGAAGCATCCAACTGCGGAGCGTCCG
TAACGGCTCTCACATGACGGACGCCGACTGGCCGAAGCTGACCAACGTCGTCAGCTATCTGTCCGAGCTGCCACTGATGA
TCGACGAGACGCCCGGCCTGACGATGGCCGAGATCGCGAGCCGCGCGCGCTCCGTCAAGCGGAAGCACGGCCTAAAGTTG
ATCGTCGTGGATTACCTGCAGCTCATGACTGGCGGCACGGACGAGCGGCACGACCTTCGGATCGCCAGCTATTCCGGCGG
CCTGAAGATGCTCGCGAAGCAGCTCGACGTGCCCGTCATCGCGCTGTCCCAACTGAACCGGCAGCTCGAGCAGCGGCCGA
ATAAGCGCCCGATCATGGCCGACCTGCGCGACTCCGGCGCAATCGAGCAGGACGCCGACACGATCATCTTCCTGTACCGG
GACGAGGTCTATCACGAGGACTCGCCAGAAGCCGGTACCGCCGAGGCAATCATCGCGAAGCAACGCAACGGCGCACTCGG
CACGGCCTATCTCGCATTCATCCATGAGCAGGCGAAGTTCGGCGACCTCGCCATGGGCTACTACCCGCAACCGCGCTCGG
CGCCTGTTAAATCGCGAGGCTTCAATGACGACTGA

Upstream 100 bases:

>100_bases
TGCGCTCACGCGCGACGATCGAGGCGAAGCTGGCGGGGGAGCCTGCGGACAGCGAGTGCGCTGCCACGCTGCGTTGGGTT
CTGGGCCAGGATTGACCGCC

Downstream 100 bases:

>100_bases
CATCACCACCGAGTTCGACGTCGCGGAGGCGCCGCCGGCGCTGGGCGGTCTGAACCTGCTGGCGCTCGACCTCGGCACGC
AGTGCGGCTGGGCGCTTTCG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MNAVENYFGDGAIDVPHSIEAEQSVLGALMIDNDSIDRIGALQQEHFFRYEHRIIFEGATKLIAVGRSADAVTVYEWLQA
HGHVEKTGGLPYLNSIVQSTPGSANMARYAEIVVERAKLRYIMAAADKVSAEVQNRNGRTADELTAFAQSQFEPLSEGRT
DGPKFIGTFLSPVVTQMDQEHHGNAPLATATGYRELDFKLGGGVRGGDLIVVAARPSMGKTAMAQGIAEYVAQTQGTALI
FSLEMPGTQLTQRAIARQGSIQLRSVRNGSHMTDADWPKLTNVVSYLSELPLMIDETPGLTMAEIASRARSVKRKHGLKL
IVVDYLQLMTGGTDERHDLRIASYSGGLKMLAKQLDVPVIALSQLNRQLEQRPNKRPIMADLRDSGAIEQDADTIIFLYR
DEVYHEDSPEAGTAEAIIAKQRNGALGTAYLAFIHEQAKFGDLAMGYYPQPRSAPVKSRGFNDD

Sequences:

>Translated_464_residues
MNAVENYFGDGAIDVPHSIEAEQSVLGALMIDNDSIDRIGALQQEHFFRYEHRIIFEGATKLIAVGRSADAVTVYEWLQA
HGHVEKTGGLPYLNSIVQSTPGSANMARYAEIVVERAKLRYIMAAADKVSAEVQNRNGRTADELTAFAQSQFEPLSEGRT
DGPKFIGTFLSPVVTQMDQEHHGNAPLATATGYRELDFKLGGGVRGGDLIVVAARPSMGKTAMAQGIAEYVAQTQGTALI
FSLEMPGTQLTQRAIARQGSIQLRSVRNGSHMTDADWPKLTNVVSYLSELPLMIDETPGLTMAEIASRARSVKRKHGLKL
IVVDYLQLMTGGTDERHDLRIASYSGGLKMLAKQLDVPVIALSQLNRQLEQRPNKRPIMADLRDSGAIEQDADTIIFLYR
DEVYHEDSPEAGTAEAIIAKQRNGALGTAYLAFIHEQAKFGDLAMGYYPQPRSAPVKSRGFNDD
>Mature_464_residues
MNAVENYFGDGAIDVPHSIEAEQSVLGALMIDNDSIDRIGALQQEHFFRYEHRIIFEGATKLIAVGRSADAVTVYEWLQA
HGHVEKTGGLPYLNSIVQSTPGSANMARYAEIVVERAKLRYIMAAADKVSAEVQNRNGRTADELTAFAQSQFEPLSEGRT
DGPKFIGTFLSPVVTQMDQEHHGNAPLATATGYRELDFKLGGGVRGGDLIVVAARPSMGKTAMAQGIAEYVAQTQGTALI
FSLEMPGTQLTQRAIARQGSIQLRSVRNGSHMTDADWPKLTNVVSYLSELPLMIDETPGLTMAEIASRARSVKRKHGLKL
IVVDYLQLMTGGTDERHDLRIASYSGGLKMLAKQLDVPVIALSQLNRQLEQRPNKRPIMADLRDSGAIEQDADTIIFLYR
DEVYHEDSPEAGTAEAIIAKQRNGALGTAYLAFIHEQAKFGDLAMGYYPQPRSAPVKSRGFNDD

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=443, Percent_Identity=42.6636568848758, Blast_Score=329, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50793; Mature: 50793

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVENYFGDGAIDVPHSIEAEQSVLGALMIDNDSIDRIGALQQEHFFRYEHRIIFEGAT
CCCHHHHCCCCCCCCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHEEEECCCC
KLIAVGRSADAVTVYEWLQAHGHVEKTGGLPYLNSIVQSTPGSANMARYAEIVVERAKLR
EEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YIMAAADKVSAEVQNRNGRTADELTAFAQSQFEPLSEGRTDGPKFIGTFLSPVVTQMDQE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
HHGNAPLATATGYRELDFKLGGGVRGGDLIVVAARPSMGKTAMAQGIAEYVAQTQGTALI
HCCCCCEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEE
FSLEMPGTQLTQRAIARQGSIQLRSVRNGSHMTDADWPKLTNVVSYLSELPLMIDETPGL
EEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCC
TMAEIASRARSVKRKHGLKLIVVDYLQLMTGGTDERHDLRIASYSGGLKMLAKQLDVPVI
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCHH
ALSQLNRQLEQRPNKRPIMADLRDSGAIEQDADTIIFLYRDEVYHEDSPEAGTAEAIIAK
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHEEC
QRNGALGTAYLAFIHEQAKFGDLAMGYYPQPRSAPVKSRGFNDD
CCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAVENYFGDGAIDVPHSIEAEQSVLGALMIDNDSIDRIGALQQEHFFRYEHRIIFEGAT
CCCHHHHCCCCCCCCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHEEEECCCC
KLIAVGRSADAVTVYEWLQAHGHVEKTGGLPYLNSIVQSTPGSANMARYAEIVVERAKLR
EEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YIMAAADKVSAEVQNRNGRTADELTAFAQSQFEPLSEGRTDGPKFIGTFLSPVVTQMDQE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
HHGNAPLATATGYRELDFKLGGGVRGGDLIVVAARPSMGKTAMAQGIAEYVAQTQGTALI
HCCCCCEEECCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEE
FSLEMPGTQLTQRAIARQGSIQLRSVRNGSHMTDADWPKLTNVVSYLSELPLMIDETPGL
EEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCC
TMAEIASRARSVKRKHGLKLIVVDYLQLMTGGTDERHDLRIASYSGGLKMLAKQLDVPVI
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCHH
ALSQLNRQLEQRPNKRPIMADLRDSGAIEQDADTIIFLYRDEVYHEDSPEAGTAEAIIAK
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHEEC
QRNGALGTAYLAFIHEQAKFGDLAMGYYPQPRSAPVKSRGFNDD
CCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]