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The map label for this gene is flgJ [H]

Identifier: 238027605

GI number: 238027605

Start: 2216247

End: 2216708

Strand: Reverse

Name: flgJ [H]

Synonym: bglu_1g20260

Alternate gene names: 238027605

Gene position: 2216708-2216247 (Counterclockwise)

Preceding gene: 238027606

Following gene: 238027604

Centisome position: 56.74

GC content: 64.94

Gene sequence:

>462_bases
GTGACCATGACACCGAATGACTTCATCAACGCGATCTCGCCGGCGGCGTGCCAACTGGCGACGTCCACCAAGATCCCGGC
GAGCTTCGTTGTTGCGCAGGCCGCGCTCGAGTCCGGGTGGGCTTCCTCGCAGCTCGCCCAGCGCTACTTCAACCTGTTTG
GGGTGAAGGCAGACGGCTCGTGGAAGGGGCCGACGGTCATGCTGCCCACGACCGAGTACGTGGCGGGGAAGCCGACCACC
GTGACAGCCAGGTGGCGCGTCTATTCCGACTGGCTCGCGAGCCTCAACGATCACGCACAGTTCCTGATCGTCAATCCCCG
CTACGCCGCCGCATTCGCGTACACCAGCGGCACGACGTTCGCGACCGCAGTCGCGGCGGCTGGCTACGCCACCGACCCGA
ACTATGCGGCGAAGATCATCGCCATCATCAAGTCGCACAACCTGAGCGCGCTCGACGGCTAA

Upstream 100 bases:

>100_bases
AACTGGCCGCGTTCACGCTGGTCGCCATCGTCATTTATCGCTACCTGCATCACGAGCCGGTGCCCGACTGGGCGCATGCG
GCCTTCACGTTCCTCTTGGG

Downstream 100 bases:

>100_bases
CGTCGCGTGTAATTCACCACCCGGCCGCCGCGAGCGGCCTTTTTTTATGCCGGAGGTTCGGATGTACATGCTGCTGGTTT
TTGTTGGTGCAGTGCTGCTG

Product: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MTMTPNDFINAISPAACQLATSTKIPASFVVAQAALESGWASSQLAQRYFNLFGVKADGSWKGPTVMLPTTEYVAGKPTT
VTARWRVYSDWLASLNDHAQFLIVNPRYAAAFAYTSGTTFATAVAAAGYATDPNYAAKIIAIIKSHNLSALDG

Sequences:

>Translated_153_residues
MTMTPNDFINAISPAACQLATSTKIPASFVVAQAALESGWASSQLAQRYFNLFGVKADGSWKGPTVMLPTTEYVAGKPTT
VTARWRVYSDWLASLNDHAQFLIVNPRYAAAFAYTSGTTFATAVAAAGYATDPNYAAKIIAIIKSHNLSALDG
>Mature_152_residues
TMTPNDFINAISPAACQLATSTKIPASFVVAQAALESGWASSQLAQRYFNLFGVKADGSWKGPTVMLPTTEYVAGKPTTV
TARWRVYSDWLASLNDHAQFLIVNPRYAAAFAYTSGTTFATAVAAAGYATDPNYAAKIIAIIKSHNLSALDG

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=142, Percent_Identity=42.9577464788732, Blast_Score=119, Evalue=6e-29,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 16273; Mature: 16142

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTPNDFINAISPAACQLATSTKIPASFVVAQAALESGWASSQLAQRYFNLFGVKADGS
CCCCCHHHHHHCCCHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCC
WKGPTVMLPTTEYVAGKPTTVTARWRVYSDWLASLNDHAQFLIVNPRYAAAFAYTSGTTF
CCCCEEEECCCHHHCCCCCEEEEEEEEHHHHHHCCCCCCEEEEECCCEEEEEEECCCCHH
ATAVAAAGYATDPNYAAKIIAIIKSHNLSALDG
HHHHHHCCCCCCCCHHHHHHEEHHCCCCCCCCC
>Mature Secondary Structure 
TMTPNDFINAISPAACQLATSTKIPASFVVAQAALESGWASSQLAQRYFNLFGVKADGS
CCCCHHHHHHCCCHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCC
WKGPTVMLPTTEYVAGKPTTVTARWRVYSDWLASLNDHAQFLIVNPRYAAAFAYTSGTTF
CCCCEEEECCCHHHCCCCCEEEEEEEEHHHHHHCCCCCCEEEEECCCEEEEEEECCCCHH
ATAVAAAGYATDPNYAAKIIAIIKSHNLSALDG
HHHHHHCCCCCCCCHHHHHHEEHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]