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The map label for this gene is rluB [H]

Identifier: 238027587

GI number: 238027587

Start: 2197701

End: 2199416

Strand: Reverse

Name: rluB [H]

Synonym: bglu_1g20050

Alternate gene names: 238027587

Gene position: 2199416-2197701 (Counterclockwise)

Preceding gene: 238027588

Following gene: 238027586

Centisome position: 56.3

GC content: 73.08

Gene sequence:

>1716_bases
TTGACAGATACCCACGACACTGATTCGTCCGAATCCGCGCAGGCCATGCAGGCGTCGCGCGCCGGCGGTGCCCCCGAGCA
GTCCGCGCAGGATGCGGGCGGCGAGGAGCGCCCGCGCCGCAGCCTGCGCCGCGGCCCGCGCAGCCTGATCGCACGCCGCC
GCGCGGCGGCCAAATCGAAGACGGCCGAGCCGGCGGCGGACGGCGCGCAAGCCGTGCCTGCCGAGGCGGTCGATGCCGAG
GCGCCCGCCGCGGCGCCGGCACCGCGCGCCGCGCGCAGCAAGGACGGCGCGCCGAAGGCGCCGCGCAAGCCCGCCGCGAA
GCGCGAAGGTGGTGCCGCGCGCGGCGGCGCGCAGGCCAAGCGCGGCGCAGCGGCAGCCGCCGCGCCGTCGGCCGAGAGCG
GCGGCGCGGGACAGGACGACATCTTCTCCTACGTGACGTCGCCGGCTTTCGACGCGGACAACGCGGCAGCCGGCGGCGGC
GTGCGCGCACCGATGCTGCGCCGCGGCCGCCAGCCGCAGAAGCGCGTGCTCGCCCCCGAAGACGACGCGCCGAAGCTGCA
CAAGGTGCTCGCCGAGGCCGGCATGGGCTCGCGGCGCGAGATGGAGGAACTGATCGTGGCCGGCCGCGTGTCGGTAAACG
GCGAGCCGGCGCATATCGGCCAGCGGATCCTGCCGACCGATCAGGTCCGAATCAACGGCAAGCCGGTCAAGCGCAAGCTG
CCGAACAAGCCGCCGCGCGTGCTGCTCTACCACAAGCCGACCGGCGAAATCGTCAGCCATGCCGATCCGGAGGGCCGCGC
CTCGGTGTTCGACCGCCTGCCGCCGATGAAGACGGCGAAATGGCTGGCGGTCGGCCGGCTCGATTTCAACACCGAAGGTC
TGCTGATGCTGACCACCTCGGGTGATCTCGCGAACCGCTTCATGCACCCGCGCTACAGCGTCGAGCGCGAATATGCGGTG
CGGGTGGTCGGCGAGCTCAGCGAAGCCTCGCGCCAGAAGCTGCTGCACGGCGTGGAACTCGACGACGGCCCGGCCAATTT
CCTGCGTATCCGCGACGGCGGCGGCGAAGGCACCAACCATTGGTATCACGTGGCGCTCGCCGAAGGCCGGAATCGCGAGG
TGCGGCGCATGTTCGAGGCGGTCGGGCTGATGGTGAGCCGCCTGATTCGCACGCGCCACGGCTCGATCTCGTTGCCGCGC
GGCCTGAAGCGGGGCCGCTGGGAGGAACTCGACGAGGCGCAGGTACGCAAGCTGATGGCGACCGTCGGCCTCAAGGTCGC
CGCCGACGAGAAGGGCGGCAAGCGCGGGGCCGGCGCGGCCGAGCGCAAGCAGCCGGACCCGATGCAGACCTCGATGGGCT
TTCTCAATCGCGAGCCGGTGCTGACCACCCACGGCCAGCTCGACCAGCCGCGTCGCGGCGGCCGTCGCGGCGCCCAGGGC
GGCGGTTTCGGTGCCGGCCTCGGCGGCGGTTTTGCCGGGGGCGGCGCGTCGGCCGGCGGCTACGGCGGCAATGCGAACGG
CAACCGTCGTGGCGCTGGCGGCGACGTCGACGGAAATCGTCTGTCCTATGGCGCCAACGGCAACAAGCGCGGCGGCGGCA
AGAGTCCACGCAGCGGCGGTGGTAATGCGAATGGCAACCGTGCCGACGGCGGCGGCAACGCGCGCGGCGGCGCACGCCCG
CAGCAGCCGCGCAGCAATCGCTCGCGCGGTCGCTGA

Upstream 100 bases:

>100_bases
TCGCCGGATCGGGATGCGCCCACGCAGCGCCGCGCTTGACGCGGCCAGATGAGATTGCCGCGCCCGCGAGGGGCGCGAGA
CCTGACATTTCGAGGTTGTT

Downstream 100 bases:

>100_bases
CTTGTCGGCGCGGCGCTGCGGCGGCGAACGCGCGGCGAAGCCAGGCGAAATCGGCGCTTCCGGCCGATTTCGCGGGGGCG
ATACTTTGCGTTTGAGTTAA

Product: RNA pseudouridine synthase family protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MTDTHDTDSSESAQAMQASRAGGAPEQSAQDAGGEERPRRSLRRGPRSLIARRRAAAKSKTAEPAADGAQAVPAEAVDAE
APAAAPAPRAARSKDGAPKAPRKPAAKREGGAARGGAQAKRGAAAAAAPSAESGGAGQDDIFSYVTSPAFDADNAAAGGG
VRAPMLRRGRQPQKRVLAPEDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRILPTDQVRINGKPVKRKL
PNKPPRVLLYHKPTGEIVSHADPEGRASVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAV
RVVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGSISLPR
GLKRGRWEELDEAQVRKLMATVGLKVAADEKGGKRGAGAAERKQPDPMQTSMGFLNREPVLTTHGQLDQPRRGGRRGAQG
GGFGAGLGGGFAGGGASAGGYGGNANGNRRGAGGDVDGNRLSYGANGNKRGGGKSPRSGGGNANGNRADGGGNARGGARP
QQPRSNRSRGR

Sequences:

>Translated_571_residues
MTDTHDTDSSESAQAMQASRAGGAPEQSAQDAGGEERPRRSLRRGPRSLIARRRAAAKSKTAEPAADGAQAVPAEAVDAE
APAAAPAPRAARSKDGAPKAPRKPAAKREGGAARGGAQAKRGAAAAAAPSAESGGAGQDDIFSYVTSPAFDADNAAAGGG
VRAPMLRRGRQPQKRVLAPEDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRILPTDQVRINGKPVKRKL
PNKPPRVLLYHKPTGEIVSHADPEGRASVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAV
RVVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGSISLPR
GLKRGRWEELDEAQVRKLMATVGLKVAADEKGGKRGAGAAERKQPDPMQTSMGFLNREPVLTTHGQLDQPRRGGRRGAQG
GGFGAGLGGGFAGGGASAGGYGGNANGNRRGAGGDVDGNRLSYGANGNKRGGGKSPRSGGGNANGNRADGGGNARGGARP
QQPRSNRSRGR
>Mature_570_residues
TDTHDTDSSESAQAMQASRAGGAPEQSAQDAGGEERPRRSLRRGPRSLIARRRAAAKSKTAEPAADGAQAVPAEAVDAEA
PAAAPAPRAARSKDGAPKAPRKPAAKREGGAARGGAQAKRGAAAAAAPSAESGGAGQDDIFSYVTSPAFDADNAAAGGGV
RAPMLRRGRQPQKRVLAPEDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRILPTDQVRINGKPVKRKLP
NKPPRVLLYHKPTGEIVSHADPEGRASVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYAVR
VVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGSISLPRG
LKRGRWEELDEAQVRKLMATVGLKVAADEKGGKRGAGAAERKQPDPMQTSMGFLNREPVLTTHGQLDQPRRGGRRGAQGG
GFGAGLGGGFAGGGASAGGYGGNANGNRRGAGGDVDGNRLSYGANGNKRGGGKSPRSGGGNANGNRADGGGNARGGARPQ
QPRSNRSRGR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=245, Percent_Identity=56.734693877551, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI1790453, Length=253, Percent_Identity=30.0395256916996, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI87081838, Length=192, Percent_Identity=34.8958333333333, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788510, Length=214, Percent_Identity=29.9065420560748, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 59827; Mature: 59696

Theoretical pI: Translated: 11.38; Mature: 11.38

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTHDTDSSESAQAMQASRAGGAPEQSAQDAGGEERPRRSLRRGPRSLIARRRAAAKSK
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHC
TAEPAADGAQAVPAEAVDAEAPAAAPAPRAARSKDGAPKAPRKPAAKREGGAARGGAQAK
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RGAAAAAAPSAESGGAGQDDIFSYVTSPAFDADNAAAGGGVRAPMLRRGRQPQKRVLAPE
HCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCC
DDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRILPTDQVRINGKPVKRKL
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCCHHHHCCCCCCCCEEEECCCCHHHHC
PNKPPRVLLYHKPTGEIVSHADPEGRASVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTS
CCCCCEEEEEECCCCHHHHCCCCCHHHHHHHCCCCCCCHHEEEEEEEEECCCCEEEEECC
GDLANRFMHPRYSVEREYAVRVVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNH
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
WYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGSISLPRGLKRGRWEELDEAQVRKLMA
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
TVGLKVAADEKGGKRGAGAAERKQPDPMQTSMGFLNREPVLTTHGQLDQPRRGGRRGAQG
HHCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHCCCCCCCC
GGFGAGLGGGFAGGGASAGGYGGNANGNRRGAGGDVDGNRLSYGANGNKRGGGKSPRSGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
GNANGNRADGGGNARGGARPQQPRSNRSRGR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDTHDTDSSESAQAMQASRAGGAPEQSAQDAGGEERPRRSLRRGPRSLIARRRAAAKSK
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHC
TAEPAADGAQAVPAEAVDAEAPAAAPAPRAARSKDGAPKAPRKPAAKREGGAARGGAQAK
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RGAAAAAAPSAESGGAGQDDIFSYVTSPAFDADNAAAGGGVRAPMLRRGRQPQKRVLAPE
HCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCC
DDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRILPTDQVRINGKPVKRKL
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCCHHHHCCCCCCCCEEEECCCCHHHHC
PNKPPRVLLYHKPTGEIVSHADPEGRASVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTS
CCCCCEEEEEECCCCHHHHCCCCCHHHHHHHCCCCCCCHHEEEEEEEEECCCCEEEEECC
GDLANRFMHPRYSVEREYAVRVVGELSEASRQKLLHGVELDDGPANFLRIRDGGGEGTNH
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
WYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGSISLPRGLKRGRWEELDEAQVRKLMA
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
TVGLKVAADEKGGKRGAGAAERKQPDPMQTSMGFLNREPVLTTHGQLDQPRRGGRRGAQG
HHCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCHHCCCCCCCC
GGFGAGLGGGFAGGGASAGGYGGNANGNRRGAGGDVDGNRLSYGANGNKRGGGKSPRSGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
GNANGNRADGGGNARGGARPQQPRSNRSRGR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]