Click here to switch to the map view.

The map label for this gene is truB [H]

Identifier: 238027582

GI number: 238027582

Start: 2190928

End: 2191884

Strand: Reverse

Name: truB [H]

Synonym: bglu_1g20000

Alternate gene names: 238027582

Gene position: 2191884-2190928 (Counterclockwise)

Preceding gene: 238027583

Following gene: 238027577

Centisome position: 56.11

GC content: 70.74

Gene sequence:

>957_bases
ATGACGACTGCTCCCCAACGCTCCCAGCGCCCGCGCGTGCCGCGGCGCCTGCTCGACGGCGTGCTGCTGCTCGACAAGCC
CGTCGGGCTGTCGAGCAATGACGCGCTGATTCGCGCCAAGCGCCTCTATCTCGCGAAGAAGGCCGGTCATACCGGCACGC
TCGATCCGCTCGCCTCCGGCCTGTTGCCGCTCTGCTTCGGCGAGGCCACCAAGTTCTCGCAGGATCTGCTCGAGGCCGAC
AAGACCTACGAGGCAACCATGCGGCTCGGCATCCGCACCACCACCGGCGACGCCGAGGGCGAGGCGATCGAGACGCGCGA
GGTGCGGGTCGGGTGCGCCGCCGTCGAGGCCGTGCTGCCACGTTTTCTCGGCGAGATCGTGCAGGTGCCGCCGATGTATT
CGGCACTCAAGCGCGACGGCAAACCGCTCTACGAGTACGCCCGCGCCGGGCAGACCGTCGAACGCGAGGGCCGCAACGTG
ACGATCCACGCGCTCGAACTGCTCGCCTGCGAATTGCCCAACGTGACGTTTCGCGTGACCTGCAGCAAGGGAACCTACGT
GCGCACGCTTGCCGAGGACATCGGCGAGGCGCTCGGCTGCGGTGCGCATCTGGTGGCGCTGCGGCGTACCGGCGTTGGCG
CCCTGACGCTCGAGCACGCGGTCACGCTCGACGCGCTGTCGGACGCGCAAGCGGCCGAGCGCGATGGCTGGCTGCAACCC
GTCGATGCGCTGCTCTCGACGTTTCCGCCGGTGCGGCTCGACGCCGAGGAGGCTAAGCGGTTCCGGCATGGTCAGCGGTT
GCGGCTCGCCTCGGCGCCCGAGACCGGCGCCCATGACGAGGCCGGGGCGCGGGTGCGGGTGTACGACGCCGCCGGGCAAC
TGCTCGGCGTGGCACGCCTGCTCGATGGGGTGCTGGCACCGGAGCGGCTCGTCGTCAGCGGCGAGGCGGCGGGCTGA

Upstream 100 bases:

>100_bases
AAAAGCAGTCGAGATGTCGCGCCTGATCGAGCAGGCGAATTCGACGCGTGCGAAGGACGACTGATTCGCGCGCCGACTTC
CGCCGATTGCCGATTTCGCC

Downstream 100 bases:

>100_bases
CGGCCCGCTGCCTCGCGTTGCCGGGCGTCGTGCTGTTCAAAGGGCGGCGGCCCGCACCGCCTTGCCGAGGCGGGCCGCCG
CCTCAATGCGCGGCCGATGC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTTAPQRSQRPRVPRRLLDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEAD
KTYEATMRLGIRTTTGDAEGEAIETREVRVGCAAVEAVLPRFLGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNV
TIHALELLACELPNVTFRVTCSKGTYVRTLAEDIGEALGCGAHLVALRRTGVGALTLEHAVTLDALSDAQAAERDGWLQP
VDALLSTFPPVRLDAEEAKRFRHGQRLRLASAPETGAHDEAGARVRVYDAAGQLLGVARLLDGVLAPERLVVSGEAAG

Sequences:

>Translated_318_residues
MTTAPQRSQRPRVPRRLLDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEAD
KTYEATMRLGIRTTTGDAEGEAIETREVRVGCAAVEAVLPRFLGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNV
TIHALELLACELPNVTFRVTCSKGTYVRTLAEDIGEALGCGAHLVALRRTGVGALTLEHAVTLDALSDAQAAERDGWLQP
VDALLSTFPPVRLDAEEAKRFRHGQRLRLASAPETGAHDEAGARVRVYDAAGQLLGVARLLDGVLAPERLVVSGEAAG
>Mature_317_residues
TTAPQRSQRPRVPRRLLDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASGLLPLCFGEATKFSQDLLEADK
TYEATMRLGIRTTTGDAEGEAIETREVRVGCAAVEAVLPRFLGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNVT
IHALELLACELPNVTFRVTCSKGTYVRTLAEDIGEALGCGAHLVALRRTGVGALTLEHAVTLDALSDAQAAERDGWLQPV
DALLSTFPPVRLDAEEAKRFRHGQRLRLASAPETGAHDEAGARVRVYDAAGQLLGVARLLDGVLAPERLVVSGEAAG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=255, Percent_Identity=35.2941176470588, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4503337, Length=304, Percent_Identity=28.2894736842105, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI215599015, Length=215, Percent_Identity=30.2325581395349, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367200, Length=315, Percent_Identity=46.984126984127, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17553978, Length=295, Percent_Identity=25.0847457627119, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=196, Percent_Identity=33.6734693877551, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=262, Percent_Identity=29.0076335877863, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281364189, Length=194, Percent_Identity=29.8969072164948, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364187, Length=194, Percent_Identity=29.8969072164948, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364185, Length=194, Percent_Identity=29.8969072164948, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364183, Length=194, Percent_Identity=29.8969072164948, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI62471759, Length=194, Percent_Identity=29.8969072164948, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17975520, Length=194, Percent_Identity=29.8969072164948, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 34177; Mature: 34045

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAPQRSQRPRVPRRLLDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASG
CCCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHC
LLPLCFGEATKFSQDLLEADKTYEATMRLGIRTTTGDAEGEAIETREVRVGCAAVEAVLP
CHHHHHCCHHHHHHHHHHHCHHHHHHHEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHH
RFLGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNVTIHALELLACELPNVTFRVT
HHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEHHHHHHHHCCCCCEEEEE
CSKGTYVRTLAEDIGEALGCGAHLVALRRTGVGALTLEHAVTLDALSDAQAAERDGWLQP
ECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCH
VDALLSTFPPVRLDAEEAKRFRHGQRLRLASAPETGAHDEAGARVRVYDAAGQLLGVARL
HHHHHHCCCCCEECHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHH
LDGVLAPERLVVSGEAAG
HHHHCCCCEEEECCCCCC
>Mature Secondary Structure 
TTAPQRSQRPRVPRRLLDGVLLLDKPVGLSSNDALIRAKRLYLAKKAGHTGTLDPLASG
CCCCCCCCCCCHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHC
LLPLCFGEATKFSQDLLEADKTYEATMRLGIRTTTGDAEGEAIETREVRVGCAAVEAVLP
CHHHHHCCHHHHHHHHHHHCHHHHHHHEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHH
RFLGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNVTIHALELLACELPNVTFRVT
HHHHHHHCCCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEHHHHHHHHCCCCCEEEEE
CSKGTYVRTLAEDIGEALGCGAHLVALRRTGVGALTLEHAVTLDALSDAQAAERDGWLQP
ECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCH
VDALLSTFPPVRLDAEEAKRFRHGQRLRLASAPETGAHDEAGARVRVYDAAGQLLGVARL
HHHHHHCCCCCEECHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHH
LDGVLAPERLVVSGEAAG
HHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA