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The map label for this gene is anfA [H]

Identifier: 238027554

GI number: 238027554

Start: 2156204

End: 2157595

Strand: Reverse

Name: anfA [H]

Synonym: bglu_1g19720

Alternate gene names: 238027554

Gene position: 2157595-2156204 (Counterclockwise)

Preceding gene: 238027555

Following gene: 238027553

Centisome position: 55.23

GC content: 68.03

Gene sequence:

>1392_bases
ATGAGAAATACACCTGCAATCGAGGGACTCGACCTGTATGTCTGGGAGGGCAAGGCGGACATCGTCGACCGCGTCGCGCG
CTGCATGGCCAGCTTCGACGTCGAGGTGATCCGTGCCGACAACGTCGTGCTGTCGCCCGAGCGGGCCGCCGCGCGGCCGT
CGCTCGCGATCATCAGCATCAGCATGATCGAGAGCGGCGCGGCCTTCCTGTCGGAATGGCAGGCCAACATCGGCATGCCG
GTGGTGTGGGTCGGCGCCGCCCGCGGCGGCGCCGACGCCGCCTCGTATCCGCCCGAGTACTCGCATATCCTGCCGCTGGA
CTTCACCTGCGCGGAGCTGCGCGCGCTGGTGACGAAGCTGGTCGCGCAACTGCGCGCGCACGCCGCGCAGGCCTCGGCGC
CGGCCATGCTGGTGGCGCATTCCGAGCCGATGCAGGCGCTGCTGCAGGAGGTGGACACGTTCGCCGACTGCGATACCAAC
GTGCTGCTGCACGGTGAGACCGGCGTGGGCAAGGAGCGCATCGCGCAACTGCTGCACGAGAAGCACTCGCGCTACCGGCA
TGGCGAATTCGTGCCGGTCAACTGCGGCGCGATTCCCGACGGCCTGTTCGAGTCGCTGTTCTTCGGCCACGCGAAGGGGT
CGTTCACGGGCGCGGTGGTCGCCCACAAGGGCTACTTCGAGCAGGCGGCCGGCGGCACGCTGTTCCTCGACGAGGTCGGC
GACCTGCCGCTCTACCAGCAGGTGAAGCTGCTGCGCGTGCTGGAGGACGGCACGGTGCTGCGGGTCGGCGCGAGCGCCCC
CATCAAGGTCGATTTCCGGCTCGTCGCCGCCAGCAACAAGAAGCTGCCGCAACTCGTGAAGGAGGGTACGTTCCGCGCCG
ATCTCTACTATCGGCTCGCGGTGATCGAGCTTGGCATCCCCTCGCTGGAGGAACGCGGCGCCGTCGACAAGATCGCGCTG
TTCAAGTCGTTCGTCGCCGAGGTGGTGGGCGAGGACCGGATCGGCACGCTGCCGGACCTGCCGTACTGGCTCGCCGACGC
GGTGGCCGACACCTACTTTCCCGGCAACGTGCGCGAGCTGCGCAACCTGGCCGAGCGGGTCGGCGTGACGGTGCGCCAGA
CCGGGGCCTGGGACGCGGCGCGGCTGCAGCGGCTGATCTCGCATGCGCGCGCCGTGCAGCCGGTGCCGGTCGAGAGCGCC
GCCGAAGTGTACGTCGACCGCAGCAAGTGGGACATGACCGAGCGCAATCGCGTGATCGCGGCACTTGACGCGAACGGTTG
GCGCCGCCAGGATACCGCGCAGCATCTTGGCATCAGCCGCAAGGTATTGTGGGAGAAGATGCGCAAGTATCAGATTTTCG
ACGAGGAACCCGAGGCGCGCGAAAGTGAGTGA

Upstream 100 bases:

>100_bases
AACCTGCTCGGCGTGCAGGTCGGGGCGGCCACCGTCCACCAGATCTCGCTGAATTGCGGGGTCGCGCAGACCGTCTACTG
AATGCTCTGACTGGTATCCG

Downstream 100 bases:

>100_bases
TAATGGCTGAATTGTGGTCAGAAATACAACTAAATCTGTGCAGGAATCTCTACATGAGTCATAAACGGCTTTTGGGGCGC
GCATTTGTTGCAGGATGGGT

Product: Fis family sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRNTPAIEGLDLYVWEGKADIVDRVARCMASFDVEVIRADNVVLSPERAAARPSLAIISISMIESGAAFLSEWQANIGMP
VVWVGAARGGADAASYPPEYSHILPLDFTCAELRALVTKLVAQLRAHAAQASAPAMLVAHSEPMQALLQEVDTFADCDTN
VLLHGETGVGKERIAQLLHEKHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVG
DLPLYQQVKLLRVLEDGTVLRVGASAPIKVDFRLVAASNKKLPQLVKEGTFRADLYYRLAVIELGIPSLEERGAVDKIAL
FKSFVAEVVGEDRIGTLPDLPYWLADAVADTYFPGNVRELRNLAERVGVTVRQTGAWDAARLQRLISHARAVQPVPVESA
AEVYVDRSKWDMTERNRVIAALDANGWRRQDTAQHLGISRKVLWEKMRKYQIFDEEPEARESE

Sequences:

>Translated_463_residues
MRNTPAIEGLDLYVWEGKADIVDRVARCMASFDVEVIRADNVVLSPERAAARPSLAIISISMIESGAAFLSEWQANIGMP
VVWVGAARGGADAASYPPEYSHILPLDFTCAELRALVTKLVAQLRAHAAQASAPAMLVAHSEPMQALLQEVDTFADCDTN
VLLHGETGVGKERIAQLLHEKHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVG
DLPLYQQVKLLRVLEDGTVLRVGASAPIKVDFRLVAASNKKLPQLVKEGTFRADLYYRLAVIELGIPSLEERGAVDKIAL
FKSFVAEVVGEDRIGTLPDLPYWLADAVADTYFPGNVRELRNLAERVGVTVRQTGAWDAARLQRLISHARAVQPVPVESA
AEVYVDRSKWDMTERNRVIAALDANGWRRQDTAQHLGISRKVLWEKMRKYQIFDEEPEARESE
>Mature_463_residues
MRNTPAIEGLDLYVWEGKADIVDRVARCMASFDVEVIRADNVVLSPERAAARPSLAIISISMIESGAAFLSEWQANIGMP
VVWVGAARGGADAASYPPEYSHILPLDFTCAELRALVTKLVAQLRAHAAQASAPAMLVAHSEPMQALLQEVDTFADCDTN
VLLHGETGVGKERIAQLLHEKHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVG
DLPLYQQVKLLRVLEDGTVLRVGASAPIKVDFRLVAASNKKLPQLVKEGTFRADLYYRLAVIELGIPSLEERGAVDKIAL
FKSFVAEVVGEDRIGTLPDLPYWLADAVADTYFPGNVRELRNLAERVGVTVRQTGAWDAARLQRLISHARAVQPVPVESA
AEVYVDRSKWDMTERNRVIAALDANGWRRQDTAQHLGISRKVLWEKMRKYQIFDEEPEARESE

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=406, Percent_Identity=34.4827586206897, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=38.006230529595, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1788550, Length=322, Percent_Identity=37.2670807453416, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1790299, Length=323, Percent_Identity=38.0804953560372, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=35.6923076923077, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1789087, Length=325, Percent_Identity=37.2307692307692, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI87081872, Length=323, Percent_Identity=34.984520123839, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1788905, Length=249, Percent_Identity=40.5622489959839, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI87082152, Length=286, Percent_Identity=38.8111888111888, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=35.0609756097561, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1787583, Length=339, Percent_Identity=33.3333333333333, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789828, Length=154, Percent_Identity=40.9090909090909, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI87081858, Length=293, Percent_Identity=29.6928327645051, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50997; Mature: 50997

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNTPAIEGLDLYVWEGKADIVDRVARCMASFDVEVIRADNVVLSPERAAARPSLAIISI
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCEEECCHHHHCCCCEEEEEE
SMIESGAAFLSEWQANIGMPVVWVGAARGGADAASYPPEYSHILPLDFTCAELRALVTKL
EHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHH
VAQLRAHAAQASAPAMLVAHSEPMQALLQEVDTFADCDTNVLLHGETGVGKERIAQLLHE
HHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
KHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVG
HHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCEEEECCCC
DLPLYQQVKLLRVLEDGTVLRVGASAPIKVDFRLVAASNKKLPQLVKEGTFRADLYYRLA
CCCHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHCCCCCHHHHEEEE
VIELGIPSLEERGAVDKIALFKSFVAEVVGEDRIGTLPDLPYWLADAVADTYFPGNVREL
EEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
RNLAERVGVTVRQTGAWDAARLQRLISHARAVQPVPVESAAEVYVDRSKWDMTERNRVIA
HHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCCCCCCCCCEEE
ALDANGWRRQDTAQHLGISRKVLWEKMRKYQIFDEEPEARESE
EECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRNTPAIEGLDLYVWEGKADIVDRVARCMASFDVEVIRADNVVLSPERAAARPSLAIISI
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEEECCEEECCHHHHCCCCEEEEEE
SMIESGAAFLSEWQANIGMPVVWVGAARGGADAASYPPEYSHILPLDFTCAELRALVTKL
EHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHH
VAQLRAHAAQASAPAMLVAHSEPMQALLQEVDTFADCDTNVLLHGETGVGKERIAQLLHE
HHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
KHSRYRHGEFVPVNCGAIPDGLFESLFFGHAKGSFTGAVVAHKGYFEQAAGGTLFLDEVG
HHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCEEEECCCC
DLPLYQQVKLLRVLEDGTVLRVGASAPIKVDFRLVAASNKKLPQLVKEGTFRADLYYRLA
CCCHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHCCCCCHHHHEEEE
VIELGIPSLEERGAVDKIALFKSFVAEVVGEDRIGTLPDLPYWLADAVADTYFPGNVREL
EEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
RNLAERVGVTVRQTGAWDAARLQRLISHARAVQPVPVESAAEVYVDRSKWDMTERNRVIA
HHHHHHHCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEEECCCCCCCCCCCEEE
ALDANGWRRQDTAQHLGISRKVLWEKMRKYQIFDEEPEARESE
EECCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]