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The map label for this gene is hsrA [H]

Identifier: 238027508

GI number: 238027508

Start: 2106364

End: 2107794

Strand: Reverse

Name: hsrA [H]

Synonym: bglu_1g19220

Alternate gene names: 238027508

Gene position: 2107794-2106364 (Counterclockwise)

Preceding gene: 238027511

Following gene: 238027507

Centisome position: 53.96

GC content: 58.35

Gene sequence:

>1431_bases
ATGGGTGGTAACGAAAAGGCGTCGCACGATCGGCGGTCCTCCCGGCCGGGATTGATTCTCCAGGCAGTGGTAGCGATAGC
GTTCTTCATGGAAAGCATTGACGGCAGCATAATTTCTACAGCGTTGCCCGTCATCGCGGCCAGTCTGCATGTCGCGCCGG
TGCAACTGAAGCTTGCATACGCGTCCTACTTTCTCGCGATGGCCACGTTCATCCCAATGAGCGGATGGTGCGCTGACCGA
TTCGGCGCCCGGGTCGTATTTTGTGCTTCCATCGCCGTTTTCACGTTCTCTTCCATTGCCTGCGCAGGCGCGACGAACCT
GACATCCTTCGTGATCGCTCGAGGCGTCCAGGGGATGGCGGGGGCGATGATGTTTCCGGTCGGACGCATGATTCTCGTCC
GCTCAATGCCCAAGAGTGAATTTGTTCGCGCGTTCGCAAACGTGACCATTCCATCCACAATCGGTGCGCTTAGCGGTCCG
GTGCTTGGCGGTGTGATCACCACTTATTGGGACTGGCGCTGGCTTTTCTGGATCAATGTACCGATCGGCATGTTGATTAT
CGTCGCGGGCTTGATATGTGTGCCAGTCTTGAAGGAGCCATCGAGCAAGTTCGACTTGTGGGGGCTTGTAATTTCCGGAG
CATCGCTCTGCTGCCTGGGATTTGGGATCACTTCGCTGAGTGGTGGACACCTGCAGGGTTATACCGCATTACTTCTGATT
GCAATCGGTGCAGTGGGGCTCTGGGTTTATTTCAGGCGGGCCGCCAGCCAGACCGCCCCGGTTCTTGATCTACGCCTTCT
GACAATCGCTACCTATCGAACGGGCATCGTGAGCGGATTCGTCATCCGGGCAGCCGGTGCCGGTGCGTTCTCGTTCTTGC
TTCCGCTGACACTGCAGGTAGGTTTCGGCCTCACGCCGCTCCAGTCGGGCACACTGACCTTCGCAAGCGCTGCAGGATTT
TTTGCGACGAAATTCGTGGCTACCGGTGTACTGAAGCGATTTGGCTTTCGGCGCGTGCTCGTGGTCAATGCCTGGCTTGG
CGGACTGGCGATCGGATGCCTCGCCTTGCTGCTGCCGAACACGCCGCATGCAATCATTCTCGTGATGTTGTTCGCGGGCG
GGCTGAGTCGTTGCCTCCAATATATGGCACTCGATACGCTTTCATATTCTAATATGCCGCCTTCCGCGACGAGCCGCGCA
TCCACCGTGGCAAGCGTTTCAAAGCAGTTCTCGACTGCCGTGGGCGTGGCGTATGCAGCGGGCATGCTGCAGGCCGTTGC
TTTCTTGCGAGATGTCCGTATCGGCGATGTCGAGAACTTCCGATGGGCGTTTCTGATGGTTGCATTGCCTGCCGGGGCGA
CCGCACTGCTTATCCGGCGTTTGGCGAAGGACGCCGGTAGCGAGGTATCGGGCCACCGGCCTGCGGGATAA

Upstream 100 bases:

>100_bases
TTTCGAATTGATTCCGAATGATTAAGTGTACGCGAATCAACGGGAAGATGCTATATTCCGCATCATAGTTCCTTTGAATC
TCGAAGAGCAAAGGAGGATC

Downstream 100 bases:

>100_bases
CGATATTCCAGTCAATCCGGAATGCAATGAGGTGGCGAAATGCGCACCAAACTTGGCCCTGACCGTGTTTACTCGCCTGA
GGTCAACGATGCAAACACAA

Product: putative multidrug efflux protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MGGNEKASHDRRSSRPGLILQAVVAIAFFMESIDGSIISTALPVIAASLHVAPVQLKLAYASYFLAMATFIPMSGWCADR
FGARVVFCASIAVFTFSSIACAGATNLTSFVIARGVQGMAGAMMFPVGRMILVRSMPKSEFVRAFANVTIPSTIGALSGP
VLGGVITTYWDWRWLFWINVPIGMLIIVAGLICVPVLKEPSSKFDLWGLVISGASLCCLGFGITSLSGGHLQGYTALLLI
AIGAVGLWVYFRRAASQTAPVLDLRLLTIATYRTGIVSGFVIRAAGAGAFSFLLPLTLQVGFGLTPLQSGTLTFASAAGF
FATKFVATGVLKRFGFRRVLVVNAWLGGLAIGCLALLLPNTPHAIILVMLFAGGLSRCLQYMALDTLSYSNMPPSATSRA
STVASVSKQFSTAVGVAYAAGMLQAVAFLRDVRIGDVENFRWAFLMVALPAGATALLIRRLAKDAGSEVSGHRPAG

Sequences:

>Translated_476_residues
MGGNEKASHDRRSSRPGLILQAVVAIAFFMESIDGSIISTALPVIAASLHVAPVQLKLAYASYFLAMATFIPMSGWCADR
FGARVVFCASIAVFTFSSIACAGATNLTSFVIARGVQGMAGAMMFPVGRMILVRSMPKSEFVRAFANVTIPSTIGALSGP
VLGGVITTYWDWRWLFWINVPIGMLIIVAGLICVPVLKEPSSKFDLWGLVISGASLCCLGFGITSLSGGHLQGYTALLLI
AIGAVGLWVYFRRAASQTAPVLDLRLLTIATYRTGIVSGFVIRAAGAGAFSFLLPLTLQVGFGLTPLQSGTLTFASAAGF
FATKFVATGVLKRFGFRRVLVVNAWLGGLAIGCLALLLPNTPHAIILVMLFAGGLSRCLQYMALDTLSYSNMPPSATSRA
STVASVSKQFSTAVGVAYAAGMLQAVAFLRDVRIGDVENFRWAFLMVALPAGATALLIRRLAKDAGSEVSGHRPAG
>Mature_475_residues
GGNEKASHDRRSSRPGLILQAVVAIAFFMESIDGSIISTALPVIAASLHVAPVQLKLAYASYFLAMATFIPMSGWCADRF
GARVVFCASIAVFTFSSIACAGATNLTSFVIARGVQGMAGAMMFPVGRMILVRSMPKSEFVRAFANVTIPSTIGALSGPV
LGGVITTYWDWRWLFWINVPIGMLIIVAGLICVPVLKEPSSKFDLWGLVISGASLCCLGFGITSLSGGHLQGYTALLLIA
IGAVGLWVYFRRAASQTAPVLDLRLLTIATYRTGIVSGFVIRAAGAGAFSFLLPLTLQVGFGLTPLQSGTLTFASAAGFF
ATKFVATGVLKRFGFRRVLVVNAWLGGLAIGCLALLLPNTPHAIILVMLFAGGLSRCLQYMALDTLSYSNMPPSATSRAS
TVASVSKQFSTAVGVAYAAGMLQAVAFLRDVRIGDVENFRWAFLMVALPAGATALLIRRLAKDAGSEVSGHRPAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=462, Percent_Identity=33.3333333333333, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1788392, Length=458, Percent_Identity=31.2227074235808, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1788710, Length=410, Percent_Identity=26.3414634146341, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081983, Length=336, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789042, Length=444, Percent_Identity=23.6486486486486, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790146, Length=158, Percent_Identity=29.1139240506329, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50158; Mature: 50027

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGNEKASHDRRSSRPGLILQAVVAIAFFMESIDGSIISTALPVIAASLHVAPVQLKLAY
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEECHHHHHHHH
ASYFLAMATFIPMSGWCADRFGARVVFCASIAVFTFSSIACAGATNLTSFVIARGVQGMA
HHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
GAMMFPVGRMILVRSMPKSEFVRAFANVTIPSTIGALSGPVLGGVITTYWDWRWLFWINV
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCEEEEEEEEC
PIGMLIIVAGLICVPVLKEPSSKFDLWGLVISGASLCCLGFGITSLSGGHLQGYTALLLI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCHHHCCCCCCHHHHHHHHH
AIGAVGLWVYFRRAASQTAPVLDLRLLTIATYRTGIVSGFVIRAAGAGAFSFLLPLTLQV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHH
GFGLTPLQSGTLTFASAAGFFATKFVATGVLKRFGFRRVLVVNAWLGGLAIGCLALLLPN
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCC
TPHAIILVMLFAGGLSRCLQYMALDTLSYSNMPPSATSRASTVASVSKQFSTAVGVAYAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GMLQAVAFLRDVRIGDVENFRWAFLMVALPAGATALLIRRLAKDAGSEVSGHRPAG
HHHHHHHHHHHCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
GGNEKASHDRRSSRPGLILQAVVAIAFFMESIDGSIISTALPVIAASLHVAPVQLKLAY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEECHHHHHHHH
ASYFLAMATFIPMSGWCADRFGARVVFCASIAVFTFSSIACAGATNLTSFVIARGVQGMA
HHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
GAMMFPVGRMILVRSMPKSEFVRAFANVTIPSTIGALSGPVLGGVITTYWDWRWLFWINV
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCEEEEEEEEC
PIGMLIIVAGLICVPVLKEPSSKFDLWGLVISGASLCCLGFGITSLSGGHLQGYTALLLI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCHHHCCCCCCHHHHHHHHH
AIGAVGLWVYFRRAASQTAPVLDLRLLTIATYRTGIVSGFVIRAAGAGAFSFLLPLTLQV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHH
GFGLTPLQSGTLTFASAAGFFATKFVATGVLKRFGFRRVLVVNAWLGGLAIGCLALLLPN
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCC
TPHAIILVMLFAGGLSRCLQYMALDTLSYSNMPPSATSRASTVASVSKQFSTAVGVAYAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GMLQAVAFLRDVRIGDVENFRWAFLMVALPAGATALLIRRLAKDAGSEVSGHRPAG
HHHHHHHHHHHCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]