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The map label for this gene is cysU [H]

Identifier: 238027469

GI number: 238027469

Start: 2055220

End: 2056119

Strand: Reverse

Name: cysU [H]

Synonym: bglu_1g18720

Alternate gene names: 238027469

Gene position: 2056119-2055220 (Counterclockwise)

Preceding gene: 238027470

Following gene: 238027468

Centisome position: 52.63

GC content: 68.89

Gene sequence:

>900_bases
ATGACGACGTACACCTTCCGCAAGCCGAGCGCGCTGCCCGGCTTCGGCGTGACGCTCGGCATCACGGTGGCCTATCTGAG
CCTCGTGGTGCTGATACCGCTCGCCTCCACCTTTCTGAAGACCGCGACGCTGACCTGGGACCAGTTCGTCGCCGCGACGA
CTTCCGCGCGCGTGCTCGCCTCGTACCGGCTCACCTTCCTTGCGGCGTTCGGCGGCGCGCTGATCAACGCCGTGTTCGGC
TTCCTGGTGGCCTGGGTGCTGGTGCGCTACCGGTTCCCGTTCCAGCGCGTCGTCGACGCGATCGTCGACCTGCCGTTCGC
GCTGCCGACCTCGGTCGCGGGCATTTCGCTGGCGGCCGTCTACGCCTCCAACGGCTGGCTCGGCCGCTATCTCGCGCCGC
TGGGCATCAAGATCGCATTCACGCCGCTCGGCGTGCTGGTGGCGCTGACCTTCATCGGGCTGCCGTTCGTGGTGCGCACG
GTGCAGCCCGTGCTCGAGGAATTCGAGCGCGAGCAGGAGGAGGCCGCCGCCTGCCTCGGCGCCTCGCGCTGGCTGACGTT
CCGCCGGGTGGTGCTGCCGGCCGTGTTCCCGGCCCTGTTGACGGGCTTCGCGCTTGCGTTCGCGCGTGCGCTCGGCGAGT
ACGGCTCGGTGATCTTCATCGCCGGAAACGTGCCGATGAAGTCCGAGATCACCTCGCTCTTGATCATCACCAAGCTCGAG
CAGTACGACTACGCGGGCGCCACCGCGATCGCGGTGGTGATGCTGGTGGTCTCGTTCCTGATGCTGCTGCTCATCAATAC
GCTGCAATGGTGGCTGCAGCGCCGCACCGGCCGCAGCGCGAGCGCTCCGGTGCCGGCCGTGGCCGGCACTGCGGCGGCCG
CCGCCGGAGGTGCGCAATGA

Upstream 100 bases:

>100_bases
ACGCGCGGCCCGATACAACGATGGACTCGAATTGACGCGCGGCCGGCCGGCACCTTCGGCGCGGCCGCGCCTCCCATGTC
AACCTAGGAAGCGCATTCGC

Downstream 100 bases:

>100_bases
GCCGCGAATCCACCTCAGCCGTGCTGCCCGCCGGCGCCGCGCCGCGTGCGAAGGCCGCGCGCCGGCGCGATCCCGTCACC
GAATCGCGGCTGGTGCGCTG

Product: sulfate ABC transporter inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLASTFLKTATLTWDQFVAATTSARVLASYRLTFLAAFGGALINAVFG
FLVAWVLVRYRFPFQRVVDAIVDLPFALPTSVAGISLAAVYASNGWLGRYLAPLGIKIAFTPLGVLVALTFIGLPFVVRT
VQPVLEEFEREQEEAAACLGASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
QYDYAGATAIAVVMLVVSFLMLLLINTLQWWLQRRTGRSASAPVPAVAGTAAAAAGGAQ

Sequences:

>Translated_299_residues
MTTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLASTFLKTATLTWDQFVAATTSARVLASYRLTFLAAFGGALINAVFG
FLVAWVLVRYRFPFQRVVDAIVDLPFALPTSVAGISLAAVYASNGWLGRYLAPLGIKIAFTPLGVLVALTFIGLPFVVRT
VQPVLEEFEREQEEAAACLGASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
QYDYAGATAIAVVMLVVSFLMLLLINTLQWWLQRRTGRSASAPVPAVAGTAAAAAGGAQ
>Mature_298_residues
TTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLASTFLKTATLTWDQFVAATTSARVLASYRLTFLAAFGGALINAVFGF
LVAWVLVRYRFPFQRVVDAIVDLPFALPTSVAGISLAAVYASNGWLGRYLAPLGIKIAFTPLGVLVALTFIGLPFVVRTV
QPVLEEFEREQEEAAACLGASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLEQ
YDYAGATAIAVVMLVVSFLMLLLINTLQWWLQRRTGRSASAPVPAVAGTAAAAAGGAQ

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=264, Percent_Identity=50.7575757575758, Blast_Score=253, Evalue=9e-69,
Organism=Escherichia coli, GI87082099, Length=265, Percent_Identity=33.5849056603774, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1786980, Length=223, Percent_Identity=31.8385650224215, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786254, Length=227, Percent_Identity=29.5154185022026, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787369, Length=198, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787368, Length=221, Percent_Identity=29.8642533936652, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31974; Mature: 31843

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLASTFLKTATLTWDQFVAATTSARVLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SYRLTFLAAFGGALINAVFGFLVAWVLVRYRFPFQRVVDAIVDLPFALPTSVAGISLAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
YASNGWLGRYLAPLGIKIAFTPLGVLVALTFIGLPFVVRTVQPVLEEFEREQEEAAACLG
HHCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
QYDYAGATAIAVVMLVVSFLMLLLINTLQWWLQRRTGRSASAPVPAVAGTAAAAAGGAQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTYTFRKPSALPGFGVTLGITVAYLSLVVLIPLASTFLKTATLTWDQFVAATTSARVLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SYRLTFLAAFGGALINAVFGFLVAWVLVRYRFPFQRVVDAIVDLPFALPTSVAGISLAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
YASNGWLGRYLAPLGIKIAFTPLGVLVALTFIGLPFVVRTVQPVLEEFEREQEEAAACLG
HHCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASRWLTFRRVVLPAVFPALLTGFALAFARALGEYGSVIFIAGNVPMKSEITSLLIITKLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
QYDYAGATAIAVVMLVVSFLMLLLINTLQWWLQRRTGRSASAPVPAVAGTAAAAAGGAQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]