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The map label for this gene is pldA [C]

Identifier: 238027221

GI number: 238027221

Start: 1758501

End: 1759790

Strand: Reverse

Name: pldA [C]

Synonym: bglu_1g16070

Alternate gene names: 238027221

Gene position: 1759790-1758501 (Counterclockwise)

Preceding gene: 238027226

Following gene: 238027220

Centisome position: 45.05

GC content: 69.3

Gene sequence:

>1290_bases
ATGCGCCATCGCTCCCGCCGTCGCTTCGCCCTGCCCCGCCGCGCGCTCGCCGCGCTGATCCCCGCTGCCTCGCTCGCCGG
CGCCGCGATACCCGCGCACGCCACCGTGTCGCTCGTGCAGCCGGCGCGCGAGGCCGCCGCCAGCGCACCGCTCACGCTGA
CGCTGATCTATGCGGACGACGACCGCGCGCCGCTCGCGGTCACCACGCCGGACTCGCTCGAGGTGACCGTTACCGGCGCC
GAGCAGGCGCCGGTAACGGTGCGGCTCGCTCGCGAGCCGGGCCTGCCGGCACGCTTCACGTTGCGCGCCGGCCAGTTCCG
CAAGGTGCGATTTTCCGCGCCATGGCCCGACAGCGCGCGCGGCACGGTGACCATCACGCCGGTCGGCTTCGACGCCTCGG
CCGCCGTGGTGTCGCTCGATCGCGGCCCGAACCAGGCGCGCGTCGCGCAGGCCGAAGCGGCGGAAGCGCGCCAGACGACA
CCGGCCGGCGTGGCCGCGGCCGCCACCCACGTCGCCGCGACGGCCGGCACGCCGCTGCCGACGGCCGACGCGCTGACCAC
CGGCTCGCAGGGGCTGCTGTCGAGAATCTCGTACTACGAACCGATGTTCATCGGCGCGGGCGTAAACGGCGACGTCACCG
CCAAGCTGCAACTGAGCTTCAAGTACCGCTTGCACATGCCCGACGATCCGTCGTCGCGCGCGCTGCTCGACAACCTGTAT
TTCGGCTATACGCAGACCTCGATCTGGGATCTGTCGGCCGATTCGAGCCCGTTCCGCGACACCAGCTACAAGCCGCGACT
GTTCTATTACGTGGCCGACACCGGCCTGCGCAGCAACTGGTTCACGCGGATGGGATTCGCGGCCGGGATCGGCCACGAGT
CGAATGGCAAGGCCGGTAGCGACTCGCGCAGCCTGAACATCGCGTTCGTGCAGCCGACCTGGGAGTTCGGCGATCTGTCG
GATTACCACCTGCAGGTGTCGCCGATGCTCTACTACTACGTCGGCATCAGCGACAACCGCGACATCGCCGACTATCGCGG
CTACATGGATCTGCTCGTCAAGTACGGCAGCCCGGACGGCTGGCAACTGGCGACGACGCTGCGCAAGGGCACCCACCACT
GGTATGGCAGCGTCGACACGCAGCTGTCGTATCCGCTCGCGAAACTGTTCGGCAATGCCTGGGGCGGCTATCTGTGGCTC
GGCTTCTTCGACGGCTACGGCGAGGATCTGCTCGACTACAACCACCGCCAGCACTGGATCGCGCGCGTCGGCGTGAGCAT
CGCGCGCTGA

Upstream 100 bases:

>100_bases
CCTGCCGGCCGACCCGACGCCTGCTTTACAATGGCCCAACTCGTCGCAGTATCCGCGTTGCGGCGCGACCTTCCACCGTG
CTTCGTCATCCCTCGAACTC

Downstream 100 bases:

>100_bases
TGTTTTACCATAAGGCTTTGCCGCATCCCTCGACCGCCACCCATCCATGAGCTCCAACCTTCACGACAAGCCGGACAGCC
CCTGCATCGGCGTCTGCTCG

Product: putative outer membrane phospholipase A

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MRHRSRRRFALPRRALAALIPAASLAGAAIPAHATVSLVQPAREAAASAPLTLTLIYADDDRAPLAVTTPDSLEVTVTGA
EQAPVTVRLAREPGLPARFTLRAGQFRKVRFSAPWPDSARGTVTITPVGFDASAAVVSLDRGPNQARVAQAEAAEARQTT
PAGVAAAATHVAATAGTPLPTADALTTGSQGLLSRISYYEPMFIGAGVNGDVTAKLQLSFKYRLHMPDDPSSRALLDNLY
FGYTQTSIWDLSADSSPFRDTSYKPRLFYYVADTGLRSNWFTRMGFAAGIGHESNGKAGSDSRSLNIAFVQPTWEFGDLS
DYHLQVSPMLYYYVGISDNRDIADYRGYMDLLVKYGSPDGWQLATTLRKGTHHWYGSVDTQLSYPLAKLFGNAWGGYLWL
GFFDGYGEDLLDYNHRQHWIARVGVSIAR

Sequences:

>Translated_429_residues
MRHRSRRRFALPRRALAALIPAASLAGAAIPAHATVSLVQPAREAAASAPLTLTLIYADDDRAPLAVTTPDSLEVTVTGA
EQAPVTVRLAREPGLPARFTLRAGQFRKVRFSAPWPDSARGTVTITPVGFDASAAVVSLDRGPNQARVAQAEAAEARQTT
PAGVAAAATHVAATAGTPLPTADALTTGSQGLLSRISYYEPMFIGAGVNGDVTAKLQLSFKYRLHMPDDPSSRALLDNLY
FGYTQTSIWDLSADSSPFRDTSYKPRLFYYVADTGLRSNWFTRMGFAAGIGHESNGKAGSDSRSLNIAFVQPTWEFGDLS
DYHLQVSPMLYYYVGISDNRDIADYRGYMDLLVKYGSPDGWQLATTLRKGTHHWYGSVDTQLSYPLAKLFGNAWGGYLWL
GFFDGYGEDLLDYNHRQHWIARVGVSIAR
>Mature_429_residues
MRHRSRRRFALPRRALAALIPAASLAGAAIPAHATVSLVQPAREAAASAPLTLTLIYADDDRAPLAVTTPDSLEVTVTGA
EQAPVTVRLAREPGLPARFTLRAGQFRKVRFSAPWPDSARGTVTITPVGFDASAAVVSLDRGPNQARVAQAEAAEARQTT
PAGVAAAATHVAATAGTPLPTADALTTGSQGLLSRISYYEPMFIGAGVNGDVTAKLQLSFKYRLHMPDDPSSRALLDNLY
FGYTQTSIWDLSADSSPFRDTSYKPRLFYYVADTGLRSNWFTRMGFAAGIGHESNGKAGSDSRSLNIAFVQPTWEFGDLS
DYHLQVSPMLYYYVGISDNRDIADYRGYMDLLVKYGSPDGWQLATTLRKGTHHWYGSVDTQLSYPLAKLFGNAWGGYLWL
GFFDGYGEDLLDYNHRQHWIARVGVSIAR

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=191, Percent_Identity=37.17277486911, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 46705; Mature: 46705

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHRSRRRFALPRRALAALIPAASLAGAAIPAHATVSLVQPAREAAASAPLTLTLIYADD
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEEEECC
DRAPLAVTTPDSLEVTVTGAEQAPVTVRLAREPGLPARFTLRAGQFRKVRFSAPWPDSAR
CCCCEEEECCCCEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCEEEEEECCCCCCCCC
GTVTITPVGFDASAAVVSLDRGPNQARVAQAEAAEARQTTPAGVAAAATHVAATAGTPLP
CEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCC
TADALTTGSQGLLSRISYYEPMFIGAGVNGDVTAKLQLSFKYRLHMPDDPSSRALLDNLY
CCHHHCCCCHHHHHHHHHCCCEEEECCCCCCEEEEEEEEEEEEEECCCCCCHHHHHHHHE
FGYTQTSIWDLSADSSPFRDTSYKPRLFYYVADTGLRSNWFTRMGFAAGIGHESNGKAGS
ECEECCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCC
DSRSLNIAFVQPTWEFGDLSDYHLQVSPMLYYYVGISDNRDIADYRGYMDLLVKYGSPDG
CCCEEEEEEEECCCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHEECCCCCC
WQLATTLRKGTHHWYGSVDTQLSYPLAKLFGNAWGGYLWLGFFDGYGEDLLDYNHRQHWI
CCHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCHHHCCCCHHHHH
ARVGVSIAR
HHHCCEECC
>Mature Secondary Structure
MRHRSRRRFALPRRALAALIPAASLAGAAIPAHATVSLVQPAREAAASAPLTLTLIYADD
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEEEECC
DRAPLAVTTPDSLEVTVTGAEQAPVTVRLAREPGLPARFTLRAGQFRKVRFSAPWPDSAR
CCCCEEEECCCCEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCEEEEEECCCCCCCCC
GTVTITPVGFDASAAVVSLDRGPNQARVAQAEAAEARQTTPAGVAAAATHVAATAGTPLP
CEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCC
TADALTTGSQGLLSRISYYEPMFIGAGVNGDVTAKLQLSFKYRLHMPDDPSSRALLDNLY
CCHHHCCCCHHHHHHHHHCCCEEEECCCCCCEEEEEEEEEEEEEECCCCCCHHHHHHHHE
FGYTQTSIWDLSADSSPFRDTSYKPRLFYYVADTGLRSNWFTRMGFAAGIGHESNGKAGS
ECEECCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCC
DSRSLNIAFVQPTWEFGDLSDYHLQVSPMLYYYVGISDNRDIADYRGYMDLLVKYGSPDG
CCCEEEEEEEECCCCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHHHHHEECCCCCC
WQLATTLRKGTHHWYGSVDTQLSYPLAKLFGNAWGGYLWLGFFDGYGEDLLDYNHRQHWI
CCHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCHHHCCCCHHHHH
ARVGVSIAR
HHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]