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The map label for this gene is psdht [H]

Identifier: 238026954

GI number: 238026954

Start: 1455068

End: 1456096

Strand: Reverse

Name: psdht [H]

Synonym: bglu_1g13290

Alternate gene names: 238026954

Gene position: 1456096-1455068 (Counterclockwise)

Preceding gene: 238026955

Following gene: 238026952

Centisome position: 37.27

GC content: 71.72

Gene sequence:

>1029_bases
ATGTCGACCCTGTCACCGCCCCCTGCCCATCCGTCCATCAACGGCGAGCCGATTCCCACGCTCGACGACATCGCCGCCGA
GCATTTCGCGCTCACCCCCTGGGTGTCGCGCACGCCGGTGTTCGAACGCGCCGAACTGCCCTCGCTGGAAACCACCCACG
TCAACCTGAAATTCGAGCTGCTGCAGGCAAGCGGCAGCTTCAAGGTGCGCAGCGCGTTCGCGAACCTGCTCGCGCTGCGC
GAGCCGCAACGGCGCAGCGGCGTGACCTGCGTGTCGTCGGGCAATCATGCGGCGGCGCTCGCCTACGCGGCCATGCGGCT
CGGCATCGGCGCGAAGGTGGTGCTGCTCGATACGGCCGACCGCGCACCGCTCGCGCTGTGCCGGCAGTACGGCGCCGAGG
TCGTGATGGCAGCCGGGCAGGCCGAAGCGTTCGAGCTCGTGCGGCGCATCGAGATCGAGGAAGGCCGGCAGTTCGTGCAT
GCGTTCAACGGCTACCGCACGATCCTCGGCACTGCCACGCTCGGCTACGAATGGGCCACCCAGACGCCCGACCTCGATGC
GGTGATCGTGCCGGTGGGCGGCGGCGGCCTCGCCGCCGGCATCGCGACGGCGCTGCGGCTCGCAAGCCCGCGGATTCGCG
TCTACGGCGTGGAGCCCGCAGGTGCCGACGTGATGAGCCGCAGCTTCGCGGCCAACCGGCCGCTCGCCTCGGGCCCCGCG
CATACCATCGCCGCGGCGCTCGCCGTGACCCACACCGAGGAATACGGTTACGAGCTGTGCCGGCGGCACCTCGAGCGGCT
CGTGCGTGTCGACGATGACGCGCTGCGCGCGGCGATGCGCTACCTGCACACGTATCTGAAGGTGGCCGTCGAGCCTGCCT
GCGCGGCGCCGATGGCGGCGCTGCTCGGACCGTTGCGCGAGACGCTGCAGGGCAGGCGCGTCGGCGTGCTGCTGCCGGGC
TCGAACCTCGATCCGGCGGCATTCGCGGCACACCTGGCCACGCCGGCCGGCGCGCGCCGCGCGCGCTGA

Upstream 100 bases:

>100_bases
ATGCCTGCGAGCGCTTTCTGCGCAAGTTCATCTACAGCATGACGCTCGCCTGATGCCATCCGGACCGGTTCGCCTGTCCG
GCGAGCCCCCGCTCTCCGCC

Downstream 100 bases:

>100_bases
ACGGCGCGCGGCGCGGCCGCGACGATATCCCCAGATTATGTTGAAGAGCCTGTGGACAAGCGCCTGACAAGCCACGCAAG
CCCTTGATTGCGCGGGCTTT

Product: Threonine dehydratase catabolic

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSTLSPPPAHPSINGEPIPTLDDIAAEHFALTPWVSRTPVFERAELPSLETTHVNLKFELLQASGSFKVRSAFANLLALR
EPQRRSGVTCVSSGNHAAALAYAAMRLGIGAKVVLLDTADRAPLALCRQYGAEVVMAAGQAEAFELVRRIEIEEGRQFVH
AFNGYRTILGTATLGYEWATQTPDLDAVIVPVGGGGLAAGIATALRLASPRIRVYGVEPAGADVMSRSFAANRPLASGPA
HTIAAALAVTHTEEYGYELCRRHLERLVRVDDDALRAAMRYLHTYLKVAVEPACAAPMAALLGPLRETLQGRRVGVLLPG
SNLDPAAFAAHLATPAGARRAR

Sequences:

>Translated_342_residues
MSTLSPPPAHPSINGEPIPTLDDIAAEHFALTPWVSRTPVFERAELPSLETTHVNLKFELLQASGSFKVRSAFANLLALR
EPQRRSGVTCVSSGNHAAALAYAAMRLGIGAKVVLLDTADRAPLALCRQYGAEVVMAAGQAEAFELVRRIEIEEGRQFVH
AFNGYRTILGTATLGYEWATQTPDLDAVIVPVGGGGLAAGIATALRLASPRIRVYGVEPAGADVMSRSFAANRPLASGPA
HTIAAALAVTHTEEYGYELCRRHLERLVRVDDDALRAAMRYLHTYLKVAVEPACAAPMAALLGPLRETLQGRRVGVLLPG
SNLDPAAFAAHLATPAGARRAR
>Mature_341_residues
STLSPPPAHPSINGEPIPTLDDIAAEHFALTPWVSRTPVFERAELPSLETTHVNLKFELLQASGSFKVRSAFANLLALRE
PQRRSGVTCVSSGNHAAALAYAAMRLGIGAKVVLLDTADRAPLALCRQYGAEVVMAAGQAEAFELVRRIEIEEGRQFVHA
FNGYRTILGTATLGYEWATQTPDLDAVIVPVGGGGLAAGIATALRLASPRIRVYGVEPAGADVMSRSFAANRPLASGPAH
TIAAALAVTHTEEYGYELCRRHLERLVRVDDDALRAAMRYLHTYLKVAVEPACAAPMAALLGPLRETLQGRRVGVLLPGS
NLDPAAFAAHLATPAGARRAR

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=313, Percent_Identity=30.9904153354633, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1789505, Length=271, Percent_Identity=35.4243542435424, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1790207, Length=305, Percent_Identity=30.8196721311475, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71991565, Length=305, Percent_Identity=30.4918032786885, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17508781, Length=310, Percent_Identity=26.7741935483871, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17537387, Length=313, Percent_Identity=30.3514376996805, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6322631, Length=314, Percent_Identity=31.8471337579618, Blast_Score=132, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6319788, Length=287, Percent_Identity=27.5261324041812, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320930, Length=271, Percent_Identity=29.520295202952, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355833, Length=311, Percent_Identity=35.3697749196142, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24645328, Length=203, Percent_Identity=35.4679802955665, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 36420; Mature: 36289

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLSPPPAHPSINGEPIPTLDDIAAEHFALTPWVSRTPVFERAELPSLETTHVNLKFEL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCHHHCCCCCCCEEEEEEEEEE
LQASGSFKVRSAFANLLALREPQRRSGVTCVSSGNHAAALAYAAMRLGIGAKVVLLDTAD
EECCCCCHHHHHHHHHHHHCCCHHHCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCC
RAPLALCRQYGAEVVMAAGQAEAFELVRRIEIEEGRQFVHAFNGYRTILGTATLGYEWAT
CCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEECC
QTPDLDAVIVPVGGGGLAAGIATALRLASPRIRVYGVEPAGADVMSRSFAANRPLASGPA
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCH
HTIAAALAVTHTEEYGYELCRRHLERLVRVDDDALRAAMRYLHTYLKVAVEPACAAPMAA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLGPLRETLQGRRVGVLLPGSNLDPAAFAAHLATPAGARRAR
HHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
STLSPPPAHPSINGEPIPTLDDIAAEHFALTPWVSRTPVFERAELPSLETTHVNLKFEL
CCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCCCCCHHHCCCCCCCEEEEEEEEEE
LQASGSFKVRSAFANLLALREPQRRSGVTCVSSGNHAAALAYAAMRLGIGAKVVLLDTAD
EECCCCCHHHHHHHHHHHHCCCHHHCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCC
RAPLALCRQYGAEVVMAAGQAEAFELVRRIEIEEGRQFVHAFNGYRTILGTATLGYEWAT
CCHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEECC
QTPDLDAVIVPVGGGGLAAGIATALRLASPRIRVYGVEPAGADVMSRSFAANRPLASGPA
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCH
HTIAAALAVTHTEEYGYELCRRHLERLVRVDDDALRAAMRYLHTYLKVAVEPACAAPMAA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLGPLRETLQGRRVGVLLPGSNLDPAAFAAHLATPAGARRAR
HHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]