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The map label for this gene is def1 [H]

Identifier: 238026898

GI number: 238026898

Start: 1389264

End: 1389797

Strand: Direct

Name: def1 [H]

Synonym: bglu_1g12660

Alternate gene names: 238026898

Gene position: 1389264-1389797 (Clockwise)

Preceding gene: 238026897

Following gene: 238026902

Centisome position: 35.56

GC content: 64.42

Gene sequence:

>534_bases
ATGATCCGCGAGATCCTCAGAATGGGCGACCCACGCCTGCTCGACGTGGCGAAGCCGGTCGAGGCGTTCGACACGCCGGC
GCTGCACGAGCTGGTGGCCGACATGTTCGAAACCATGCATCACGCGAACGGCGCCGGCCTCGCCGCGCCGCAGATCGGCG
TCGGGCTGCAGCTGATCATCTTCGGCTTCGGCAACAACGCGCGCTATCCCGACGCGCCGGCCGTGCCGGAAACGGTGCTG
ATCAATCCGAGCATCGAATACCTGCCGCCGGACATGGAGGAGGGCTGGGAAGGCTGCCTGTCGGTGCCGGGCATGCGCGG
CGTGGTGAGCCGCTACCGCAAGGTGCATTACACGGGCTTCGACCAATACGGCAAGCGCATCGACCGCATCGCCGAGGATT
TTCACGCGCGCGTGGTCCAGCACGAATACGATCATCTGATCGGCAAGCTCTATCCGATGCGGATCACCGATTTCTCGAAG
TTCGGCTTCACCGACGTGCTGTTCCCGGGGCTCGATCCGAACTCGGACGACTGA

Upstream 100 bases:

>100_bases
GCGCGGAGGCGGGCAGCAAGCTCGCGAAGGCCGAGGAACTCGGCATCCCGGTGCTCGACGAGGACGGGATGCATCAACTG
CTGAAAGGGGAATCACCGTC

Downstream 100 bases:

>100_bases
ACGGGCGGCGCGCCGTGAGCAGGCGCGGGAGATGGCTGGCGGCGCGGCCAGGCCGCCGCGCGCGCCGGATCGTGGGCGGC
GCCGCGCGGCGCCGCCGTCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIREILRMGDPRLLDVAKPVEAFDTPALHELVADMFETMHHANGAGLAAPQIGVGLQLIIFGFGNNARYPDAPAVPETVL
INPSIEYLPPDMEEGWEGCLSVPGMRGVVSRYRKVHYTGFDQYGKRIDRIAEDFHARVVQHEYDHLIGKLYPMRITDFSK
FGFTDVLFPGLDPNSDD

Sequences:

>Translated_177_residues
MIREILRMGDPRLLDVAKPVEAFDTPALHELVADMFETMHHANGAGLAAPQIGVGLQLIIFGFGNNARYPDAPAVPETVL
INPSIEYLPPDMEEGWEGCLSVPGMRGVVSRYRKVHYTGFDQYGKRIDRIAEDFHARVVQHEYDHLIGKLYPMRITDFSK
FGFTDVLFPGLDPNSDD
>Mature_177_residues
MIREILRMGDPRLLDVAKPVEAFDTPALHELVADMFETMHHANGAGLAAPQIGVGLQLIIFGFGNNARYPDAPAVPETVL
INPSIEYLPPDMEEGWEGCLSVPGMRGVVSRYRKVHYTGFDQYGKRIDRIAEDFHARVVQHEYDHLIGKLYPMRITDFSK
FGFTDVLFPGLDPNSDD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=31.4285714285714, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=39.5973154362416, Blast_Score=94, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24645728, Length=171, Percent_Identity=32.1637426900585, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24645726, Length=169, Percent_Identity=34.9112426035503, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19858; Mature: 19858

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREILRMGDPRLLDVAKPVEAFDTPALHELVADMFETMHHANGAGLAAPQIGVGLQLII
CHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
FGFGNNARYPDAPAVPETVLINPSIEYLPPDMEEGWEGCLSVPGMRGVVSRYRKVHYTGF
EECCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCH
DQYGKRIDRIAEDFHARVVQHEYDHLIGKLYPMRITDFSKFGFTDVLFPGLDPNSDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MIREILRMGDPRLLDVAKPVEAFDTPALHELVADMFETMHHANGAGLAAPQIGVGLQLII
CHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
FGFGNNARYPDAPAVPETVLINPSIEYLPPDMEEGWEGCLSVPGMRGVVSRYRKVHYTGF
EECCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCH
DQYGKRIDRIAEDFHARVVQHEYDHLIGKLYPMRITDFSKFGFTDVLFPGLDPNSDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]