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The map label for this gene is ligA [H]

Identifier: 238026897

GI number: 238026897

Start: 1387024

End: 1389267

Strand: Direct

Name: ligA [H]

Synonym: bglu_1g12650

Alternate gene names: 238026897

Gene position: 1387024-1389267 (Clockwise)

Preceding gene: 238026896

Following gene: 238026898

Centisome position: 35.51

GC content: 70.32

Gene sequence:

>2244_bases
ATGGCCCGAACCACCCCAGCCGACCCCCCCGCCAGCCAGCCGTCCGAGCGCGCGGCGTGGCTGCGCCAGGAACTCGAGCG
CGCCAACCACGCGTATTACGTGCTCGACCAGCCCGAACTGCTCGATGCCGAGTACGACCGGCTGTTTCGCGAACTGCAGC
AGATCGAGGCCGAGCACCCCGACTTCATCACTCCCGATTCGCCGACCCAGCGCGTGGGCGGCGAGGCCGCCGACGGCTTC
ACGCCGGTGCTCCACGACGCGCCGATGCTCTCGCTCAACAACGGCTTCACCGACGAGGACATCGCCGCGTTCGACAAGCG
GGTGGCCGACGCGCTCGGCAAGGCGGTCGATCTGGCCGGCACCGCCGAGCCGGTCGAATACGCCTGCGAGCTGAAATTCG
ACGGGCTCGCGATCTCGCTGCGCTACGTGCATGGCAAGTTCACGCAGGCCGCCACGCGCGGCGACGGCACCACCGGCGAA
GACGTGACCGAGAACGTGCGCACCATCAAGTCGATTCCGCTGACGCTGAAGGGCAAGCATCCGCCCGCGCTGCTCGACGT
GCGCGGCGAGGTGCTGATGTACAAGCGCGATTTCGCGCGCCTCAACGAGCGCCAGCGCGCGGCCGAGCAGCGCGAGTTCG
CCAACCCGCGCAACGCGGCGGCCGGCAGCCTGCGCCAGCTCGATTCGAAGGTCACCGCGCAGCGCCCGCTGTCGTTCTTC
GCCTATGGCATCGGCGTGCTCGACGGCGCGCCGATGCCCGACACGCACAGCGGCCTGCTCGACTGGTACGAGTCGCTCGG
CCTGCCGGTCAATCGCGAGCGCGCCGTGGTGCAGGGCGCCGAAGGGCTGCTCGACTTCTTCCGCAAGGTCGGCGAGAAGC
GCGAGGCGCTGCCCTACGACATCGACGGGGTGGTCTACAAGGTCAACCGGCGCGACGAGCAGGACCGGCTCGGCTTCGTC
TCGCGCGCGCCGCGCTTCGCGCTCGCGCACAAGTTCCCGGCCCAGGAGGCGCGCACGAGGCTCGTCGCGATCGAGGTGCA
GGTGGGCCGCACCGGCGCGATCACGCCGGTCGCGCGGCTCGAGCCGGTGTTCGTGGGCGGCGCGACCGTCACCAACGCGA
CGCTGCACAACGAGGACGAAGTGCGCCGCAAGGACATCCGGGTCGGCGACACGGTGATCGTGCGGCGCGCCGGCGACGTG
ATTCCCGAGGTGGTCGGCCGGGACGGCCCGCACGCGATGCAGGTGCGGGTCCGGTTTCGCGGGGTGCGGACGGTCGCGCG
GCGCAACCGGCTGCGCGCGGCCGCCTCGTCGGGCCGGCCGATGACCTGGAAGCCGCGGCTGCTGAAGCCGACGGATCGCC
GCGGCATCCGGGTGCGGCGGGGATGCCTGCGGCCGAGCCTGTTCGAGTTCGTCGAGCAGGAACCGTTCAGGATGCCGACC
GAATGCCCCGTGTGCGGCTCGAGGATCGAGCGCCTGCCCGACGAGGCGATCGCGCGCTGCACGGGCGGGCTGTTCTGCCC
CGCGCAGCGCAAGCAGGCGCTCTGGCATTTCGCGCAGCGCCGCGCGCTCGACATCGACGGGCTAGGCGAGAAGATCATCG
ACCAATTGGTCGAGCAGAACCTCGTGCGCACCCCGGCCGACCTGTTCCATCTCGGCTTCGCGACGCTCGCCGCGCTCGAC
CGCTTCGCCGAGAAGTCCGCGCAGAACCTGCTCGATTCACTCGAGAAGGCGCGCCACACCACGCTCGCGCGCTTCATCTA
CGCGCTCGGCATCCGCCACGTCGGCGAATCGACCGCGAAGGACCTGGCCAAGCACTTCGGCGCGCTGCAGCCGATCATGG
ATGCCTCGATCGACGCTCTGCTCGAAGTCAACGACGTCGGCCCGGTGGTGGCCGAATCGCTGCATCAGTTCTTTGCCGAG
GAACACAACCGCACCGTGATCGCGCAGCTGCGCGAGAAGGTGGACTGGCCCGAGGGGCCGCCCGCGCCGCGCGAGCCGCA
GGGCGTGCTGGCCGGCAAGACCGTGGTGCTGACGGGCACGCTGCCCACGCTCTCGCGCGACGAAGCGAAGGCGCTGCTCG
AGGCGGCCGGCGCGAAGGTGACGGGTTCGGTCTCGAAGAAGACCGACTACGTGGTGGCCGGCGCGGAGGCGGGCAGCAAG
CTCGCGAAGGCCGAGGAACTCGGCATCCCGGTGCTCGACGAGGACGGGATGCATCAACTGCTGAAAGGGGAATCACCGTC
ATGA

Upstream 100 bases:

>100_bases
GGTTGAGCATCGCGGGTTTCTCCCATTCGGCAGGCGCGCCAAAGCGCGCGCCGCCTGAGATAATCGTCTCCTGATTGTCA
TCCGGAAGATTGCCGCCAGC

Downstream 100 bases:

>100_bases
TCCGCGAGATCCTCAGAATGGGCGACCCACGCCTGCTCGACGTGGCGAAGCCGGTCGAGGCGTTCGACACGCCGGCGCTG
CACGAGCTGGTGGCCGACAT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 747; Mature: 746

Protein sequence:

>747_residues
MARTTPADPPASQPSERAAWLRQELERANHAYYVLDQPELLDAEYDRLFRELQQIEAEHPDFITPDSPTQRVGGEAADGF
TPVLHDAPMLSLNNGFTDEDIAAFDKRVADALGKAVDLAGTAEPVEYACELKFDGLAISLRYVHGKFTQAATRGDGTTGE
DVTENVRTIKSIPLTLKGKHPPALLDVRGEVLMYKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKVTAQRPLSFF
AYGIGVLDGAPMPDTHSGLLDWYESLGLPVNRERAVVQGAEGLLDFFRKVGEKREALPYDIDGVVYKVNRRDEQDRLGFV
SRAPRFALAHKFPAQEARTRLVAIEVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRVGDTVIVRRAGDV
IPEVVGRDGPHAMQVRVRFRGVRTVARRNRLRAAASSGRPMTWKPRLLKPTDRRGIRVRRGCLRPSLFEFVEQEPFRMPT
ECPVCGSRIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVEQNLVRTPADLFHLGFATLAALD
RFAEKSAQNLLDSLEKARHTTLARFIYALGIRHVGESTAKDLAKHFGALQPIMDASIDALLEVNDVGPVVAESLHQFFAE
EHNRTVIAQLREKVDWPEGPPAPREPQGVLAGKTVVLTGTLPTLSRDEAKALLEAAGAKVTGSVSKKTDYVVAGAEAGSK
LAKAEELGIPVLDEDGMHQLLKGESPS

Sequences:

>Translated_747_residues
MARTTPADPPASQPSERAAWLRQELERANHAYYVLDQPELLDAEYDRLFRELQQIEAEHPDFITPDSPTQRVGGEAADGF
TPVLHDAPMLSLNNGFTDEDIAAFDKRVADALGKAVDLAGTAEPVEYACELKFDGLAISLRYVHGKFTQAATRGDGTTGE
DVTENVRTIKSIPLTLKGKHPPALLDVRGEVLMYKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKVTAQRPLSFF
AYGIGVLDGAPMPDTHSGLLDWYESLGLPVNRERAVVQGAEGLLDFFRKVGEKREALPYDIDGVVYKVNRRDEQDRLGFV
SRAPRFALAHKFPAQEARTRLVAIEVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRVGDTVIVRRAGDV
IPEVVGRDGPHAMQVRVRFRGVRTVARRNRLRAAASSGRPMTWKPRLLKPTDRRGIRVRRGCLRPSLFEFVEQEPFRMPT
ECPVCGSRIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVEQNLVRTPADLFHLGFATLAALD
RFAEKSAQNLLDSLEKARHTTLARFIYALGIRHVGESTAKDLAKHFGALQPIMDASIDALLEVNDVGPVVAESLHQFFAE
EHNRTVIAQLREKVDWPEGPPAPREPQGVLAGKTVVLTGTLPTLSRDEAKALLEAAGAKVTGSVSKKTDYVVAGAEAGSK
LAKAEELGIPVLDEDGMHQLLKGESPS
>Mature_746_residues
ARTTPADPPASQPSERAAWLRQELERANHAYYVLDQPELLDAEYDRLFRELQQIEAEHPDFITPDSPTQRVGGEAADGFT
PVLHDAPMLSLNNGFTDEDIAAFDKRVADALGKAVDLAGTAEPVEYACELKFDGLAISLRYVHGKFTQAATRGDGTTGED
VTENVRTIKSIPLTLKGKHPPALLDVRGEVLMYKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKVTAQRPLSFFA
YGIGVLDGAPMPDTHSGLLDWYESLGLPVNRERAVVQGAEGLLDFFRKVGEKREALPYDIDGVVYKVNRRDEQDRLGFVS
RAPRFALAHKFPAQEARTRLVAIEVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRVGDTVIVRRAGDVI
PEVVGRDGPHAMQVRVRFRGVRTVARRNRLRAAASSGRPMTWKPRLLKPTDRRGIRVRRGCLRPSLFEFVEQEPFRMPTE
CPVCGSRIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVEQNLVRTPADLFHLGFATLAALDR
FAEKSAQNLLDSLEKARHTTLARFIYALGIRHVGESTAKDLAKHFGALQPIMDASIDALLEVNDVGPVVAESLHQFFAEE
HNRTVIAQLREKVDWPEGPPAPREPQGVLAGKTVVLTGTLPTLSRDEAKALLEAAGAKVTGSVSKKTDYVVAGAEAGSKL
AKAEELGIPVLDEDGMHQLLKGESPS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=92, Percent_Identity=46.7391304347826, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788750, Length=728, Percent_Identity=51.0989010989011, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=404, Percent_Identity=25.2475247524752, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324791, Length=89, Percent_Identity=47.1910112359551, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI62472391, Length=84, Percent_Identity=45.2380952380952, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17737765, Length=84, Percent_Identity=45.2380952380952, Blast_Score=70, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 82335; Mature: 82203

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTTPADPPASQPSERAAWLRQELERANHAYYVLDQPELLDAEYDRLFRELQQIEAEHP
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHCHHHHHHHHHHHHHHCCCC
DFITPDSPTQRVGGEAADGFTPVLHDAPMLSLNNGFTDEDIAAFDKRVADALGKAVDLAG
CCCCCCCCHHHCCCCCCCCCCHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
TAEPVEYACELKFDGLAISLRYVHGKFTQAATRGDGTTGEDVTENVRTIKSIPLTLKGKH
CCCCHHHEEEEEECCEEEEEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEEECCC
PPALLDVRGEVLMYKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKVTAQRPLSFF
CCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHH
AYGIGVLDGAPMPDTHSGLLDWYESLGLPVNRERAVVQGAEGLLDFFRKVGEKREALPYD
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCC
IDGVVYKVNRRDEQDRLGFVSRAPRFALAHKFPAQEARTRLVAIEVQVGRTGAITPVARL
CCCEEEEECCCCCHHHHHHHHCCCHHHHHHCCCHHHHCCEEEEEEEEECCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRVGDTVIVRRAGDVIPEVVGRDGPHAMQVRVRFR
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCEEEEEHHHH
GVRTVARRNRLRAAASSGRPMTWKPRLLKPTDRRGIRVRRGCLRPSLFEFVEQEPFRMPT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCC
ECPVCGSRIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVEQN
CCCHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LVRTPADLFHLGFATLAALDRFAEKSAQNLLDSLEKARHTTLARFIYALGIRHVGESTAK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
DLAKHFGALQPIMDASIDALLEVNDVGPVVAESLHQFFAEEHNRTVIAQLREKVDWPEGP
HHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
PAPREPQGVLAGKTVVLTGTLPTLSRDEAKALLEAAGAKVTGSVSKKTDYVVAGAEAGSK
CCCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEEECCCCCHH
LAKAEELGIPVLDEDGMHQLLKGESPS
HHHHHHCCCCCCCCCCHHHHHCCCCCC
>Mature Secondary Structure 
ARTTPADPPASQPSERAAWLRQELERANHAYYVLDQPELLDAEYDRLFRELQQIEAEHP
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHCHHHHHHHHHHHHHHCCCC
DFITPDSPTQRVGGEAADGFTPVLHDAPMLSLNNGFTDEDIAAFDKRVADALGKAVDLAG
CCCCCCCCHHHCCCCCCCCCCHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
TAEPVEYACELKFDGLAISLRYVHGKFTQAATRGDGTTGEDVTENVRTIKSIPLTLKGKH
CCCCHHHEEEEEECCEEEEEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEEECCC
PPALLDVRGEVLMYKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKVTAQRPLSFF
CCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHH
AYGIGVLDGAPMPDTHSGLLDWYESLGLPVNRERAVVQGAEGLLDFFRKVGEKREALPYD
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCC
IDGVVYKVNRRDEQDRLGFVSRAPRFALAHKFPAQEARTRLVAIEVQVGRTGAITPVARL
CCCEEEEECCCCCHHHHHHHHCCCHHHHHHCCCHHHHCCEEEEEEEEECCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRVGDTVIVRRAGDVIPEVVGRDGPHAMQVRVRFR
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCEEEEEHHHH
GVRTVARRNRLRAAASSGRPMTWKPRLLKPTDRRGIRVRRGCLRPSLFEFVEQEPFRMPT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCC
ECPVCGSRIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVEQN
CCCHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LVRTPADLFHLGFATLAALDRFAEKSAQNLLDSLEKARHTTLARFIYALGIRHVGESTAK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
DLAKHFGALQPIMDASIDALLEVNDVGPVVAESLHQFFAEEHNRTVIAQLREKVDWPEGP
HHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
PAPREPQGVLAGKTVVLTGTLPTLSRDEAKALLEAAGAKVTGSVSKKTDYVVAGAEAGSK
CCCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHCCCCEECCCCCCCCEEEECCCCCHH
LAKAEELGIPVLDEDGMHQLLKGESPS
HHHHHHCCCCCCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA