Click here to switch to the map view.

The map label for this gene is lutR [H]

Identifier: 238026642

GI number: 238026642

Start: 1068997

End: 1069755

Strand: Direct

Name: lutR [H]

Synonym: bglu_1g09940

Alternate gene names: 238026642

Gene position: 1068997-1069755 (Clockwise)

Preceding gene: 238026641

Following gene: 238026643

Centisome position: 27.36

GC content: 71.15

Gene sequence:

>759_bases
ATGTCCGTGCCCGCGCTCCCCGTGCCCCCGCGCCGCCGCGCCCGCAGCCTCGCCCAAGATGTCGTCGACGCGCTGAGCTC
GCAGATCGAGAACGGCATGCTGCGTCCCGGCGACAAGCTGCCGACCGAGACGGAAGTGATGGCCACCCAGGGGGTGAGCC
GCACGGTGGTGCGGGAGGCGATCTCGCGGATGCAGGCGAGCGGCCTGATCGAGACGCGCCACGGGATCGGCAGTTTCGTG
CTGGCGCCGGAGCGGCGCCGCACGCTCGGCATCGATCCGGCCACCATCACCACGCTGCGCGACGTGCTCGCCGTGCTCGA
ATTGCGCATCAGCCTCGAGAGCGAATGCGCGAGCCTCGCCGCGCAGCGCGCCAACGATACCGACCTCGATTCGCTGCGCC
GCGCGCTCGACGCGATCGCGCAGAGCGCCTCGGGCGGCCGCGACACGGCCCAGCTCGATTTCCATTTTCATCAGCAGATC
GCCCAGGCCACCGGCAACCGCTATTTCGTCGACATCATGACCCAGCTCGGCACCTCGATCATTCCGCGCGCGCGCGTGAA
CTCGGCGCGCTTCGCCGGCGACGATCTCGAACGCTATGTCGGGCGCCTGAACCACGAGCACGAGGCGATCTTCGAGGCGA
TCGCGCGGCGCGATCCGGAGGCGGCGCGCGCCGCGATGCGCACCCACCTGACCAACAGCCGCGAGCGTCTGCGCCGCGCG
CACGAAGCGGCCGAGGCGGGCCGCGACGGCGCCGATTGA

Upstream 100 bases:

>100_bases
TCAGTCATCGGATGACTTGTGACGCAGTATAATGGCCGCGCCATTTCGGTCTGATCCGCTTTCCGTCGGGTCGGATTCTC
TATTTTAAGGATAAGGCCCC

Downstream 100 bases:

>100_bases
GCGTGCCCGACCGGGCGGCCCGCCGCGCGGCGCGGCGCCCGACCCGCGTGAGGCGGAGGCCTCGCGGCTAGGGGTTCTCG
CCGGGTAGCCCAAAACGTCA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSVPALPVPPRRRARSLAQDVVDALSSQIENGMLRPGDKLPTETEVMATQGVSRTVVREAISRMQASGLIETRHGIGSFV
LAPERRRTLGIDPATITTLRDVLAVLELRISLESECASLAAQRANDTDLDSLRRALDAIAQSASGGRDTAQLDFHFHQQI
AQATGNRYFVDIMTQLGTSIIPRARVNSARFAGDDLERYVGRLNHEHEAIFEAIARRDPEAARAAMRTHLTNSRERLRRA
HEAAEAGRDGAD

Sequences:

>Translated_252_residues
MSVPALPVPPRRRARSLAQDVVDALSSQIENGMLRPGDKLPTETEVMATQGVSRTVVREAISRMQASGLIETRHGIGSFV
LAPERRRTLGIDPATITTLRDVLAVLELRISLESECASLAAQRANDTDLDSLRRALDAIAQSASGGRDTAQLDFHFHQQI
AQATGNRYFVDIMTQLGTSIIPRARVNSARFAGDDLERYVGRLNHEHEAIFEAIARRDPEAARAAMRTHLTNSRERLRRA
HEAAEAGRDGAD
>Mature_251_residues
SVPALPVPPRRRARSLAQDVVDALSSQIENGMLRPGDKLPTETEVMATQGVSRTVVREAISRMQASGLIETRHGIGSFVL
APERRRTLGIDPATITTLRDVLAVLELRISLESECASLAAQRANDTDLDSLRRALDAIAQSASGGRDTAQLDFHFHQQIA
QATGNRYFVDIMTQLGTSIIPRARVNSARFAGDDLERYVGRLNHEHEAIFEAIARRDPEAARAAMRTHLTNSRERLRRAH
EAAEAGRDGAD

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082208, Length=226, Percent_Identity=32.3008849557522, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1790780, Length=227, Percent_Identity=31.2775330396476, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790032, Length=244, Percent_Identity=28.6885245901639, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48994955, Length=224, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789621, Length=224, Percent_Identity=28.125, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789353, Length=232, Percent_Identity=26.2931034482759, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786303, Length=226, Percent_Identity=32.3008849557522, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27665; Mature: 27533

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPALPVPPRRRARSLAQDVVDALSSQIENGMLRPGDKLPTETEVMATQGVSRTVVREA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHH
ISRMQASGLIETRHGIGSFVLAPERRRTLGIDPATITTLRDVLAVLELRISLESECASLA
HHHHHHHCCHHHHHCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRANDTDLDSLRRALDAIAQSASGGRDTAQLDFHFHQQIAQATGNRYFVDIMTQLGTSI
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHH
IPRARVNSARFAGDDLERYVGRLNHEHEAIFEAIARRDPEAARAAMRTHLTNSRERLRRA
CCHHHCCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
HEAAEAGRDGAD
HHHHHCCCCCCC
>Mature Secondary Structure 
SVPALPVPPRRRARSLAQDVVDALSSQIENGMLRPGDKLPTETEVMATQGVSRTVVREA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHH
ISRMQASGLIETRHGIGSFVLAPERRRTLGIDPATITTLRDVLAVLELRISLESECASLA
HHHHHHHCCHHHHHCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRANDTDLDSLRRALDAIAQSASGGRDTAQLDFHFHQQIAQATGNRYFVDIMTQLGTSI
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHH
IPRARVNSARFAGDDLERYVGRLNHEHEAIFEAIARRDPEAARAAMRTHLTNSRERLRRA
CCHHHCCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
HEAAEAGRDGAD
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]