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The map label for this gene is mii [H]

Identifier: 238025476

GI number: 238025476

Start: 2690810

End: 2692000

Strand: Reverse

Name: mii [H]

Synonym: bglu_2g21680

Alternate gene names: 238025476

Gene position: 2692000-2690810 (Counterclockwise)

Preceding gene: 238025477

Following gene: 238025475

Centisome position: 95.21

GC content: 71.28

Gene sequence:

>1191_bases
ATGGCCCACGTTCCCCAGATCCGGATACCCGCCGTCTACATGCGCGGCGGCACCAGCAAGGGCGTGTTCTTCCGCCTCGA
CGACCTGCCGGAGGCCGCGCGCGTGCCGGGCGCGGCGCGCGACCGGCTGCTGCTGCGCGTGATCGGCAGTCCCGACCCTT
ACGGCAAGCAGATCGACGGGATGGGCGGCGCGACGTCGAGCACCAGCAAGACCGTGATCCTCTCGAAGAGCGCGCGGCCC
GGTCACGACGTCGACTATCTGTTCGGCCAGGTCTCGATCGACAAGGCCTTCATCGACTGGACCGGCAACTGCGGGAACCT
GTCGGCCGCGGTCGGCCCGTTCGCGATCGCGGCGGGCCTCGTCGACGCGTCGCGCGTGCCCGCCGACGGCATCGCCACCG
TGCGGATCTGGCAGGCCAACATCGGCAAGACCATCGTCGCGCACGTGCCGATCACGGGCGGCGAGGTGCAGGAGACGGGC
GACTTCGAACTCGACGGCGTGACTTTCCCGGCCGCCGAGATCCAGCTCGAATTCCTGGACCCGGCCGCCGACGAGGACGG
TGCCGGCGGCGCGATGTTCCCCACCGGCAACCTGGTCGACGACCTGGAAGTGCCCGGCATCGGCACGCTGAAGGCGACCA
TGATCAACGCCGGCATCCCGACCATCTTCGTCGAGGCCGAGGCGATTGGCTACACCGGCACCGAGTTGCAGGACGCGATC
AACGGCGATTGCGCCGCGCTCGCGCGCCTCGAGGCGATCCGCGGCTACGGCGCGCTGCGCATGGGCCTCATCGGGACGCT
CGACGAGATCGCCACGCGCCAGCACACGCCGAAGATCGCGTTCGTGGCGCGCCCGGCCGACTACGTCGCGTCGAGCGGCA
AGACGGTGGCGGCCAAGGACGTGGACCTGCTGGTGCGTGCGATGTCGATGGGCAAGCTGCATCACGCGATGATGGGCACC
GCCGCGGTGGCGATCGGCACCGCGGCCGCGATTCCCGGCACGCTCGTGAGCCTCGCGGCGGGCGGCGGCGAGCGCGAGGC
GGTGCGCTTCGGGCATCCGTCCGGCACGCTGCGGGTGGGCGCGCAGGCCAGCCGCGCGAGCGGCGAGTGGGTCGTCACGA
AGGCGATCATGAGCCGCAGCGCGCGCGTGCTGATGGAAGGCCAGGTGCGCGTGCCCGGCGACGCGTTCTGA

Upstream 100 bases:

>100_bases
GGCGCGACGCGTTCGAGACTCGATTAACCGGCGTGGCGCCCCGACCGGCGCGACGCGCGCGGGCGGCGCGGCCGCCCTCG
TCCTTTCAGGAACCTCCACC

Downstream 100 bases:

>100_bases
GGCTCGCGGCAGCCCGCGCGGCGTGGGTGCGTGCGGGCGCTGACGTGACAGCGGGCGCGGCCGCGACGCGGCGCGCCGAA
CGGAGGCCGGAATGAACGTG

Product: putative AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAHVPQIRIPAVYMRGGTSKGVFFRLDDLPEAARVPGAARDRLLLRVIGSPDPYGKQIDGMGGATSSTSKTVILSKSARP
GHDVDYLFGQVSIDKAFIDWTGNCGNLSAAVGPFAIAAGLVDASRVPADGIATVRIWQANIGKTIVAHVPITGGEVQETG
DFELDGVTFPAAEIQLEFLDPAADEDGAGGAMFPTGNLVDDLEVPGIGTLKATMINAGIPTIFVEAEAIGYTGTELQDAI
NGDCAALARLEAIRGYGALRMGLIGTLDEIATRQHTPKIAFVARPADYVASSGKTVAAKDVDLLVRAMSMGKLHHAMMGT
AAVAIGTAAAIPGTLVSLAAGGGEREAVRFGHPSGTLRVGAQASRASGEWVVTKAIMSRSARVLMEGQVRVPGDAF

Sequences:

>Translated_396_residues
MAHVPQIRIPAVYMRGGTSKGVFFRLDDLPEAARVPGAARDRLLLRVIGSPDPYGKQIDGMGGATSSTSKTVILSKSARP
GHDVDYLFGQVSIDKAFIDWTGNCGNLSAAVGPFAIAAGLVDASRVPADGIATVRIWQANIGKTIVAHVPITGGEVQETG
DFELDGVTFPAAEIQLEFLDPAADEDGAGGAMFPTGNLVDDLEVPGIGTLKATMINAGIPTIFVEAEAIGYTGTELQDAI
NGDCAALARLEAIRGYGALRMGLIGTLDEIATRQHTPKIAFVARPADYVASSGKTVAAKDVDLLVRAMSMGKLHHAMMGT
AAVAIGTAAAIPGTLVSLAAGGGEREAVRFGHPSGTLRVGAQASRASGEWVVTKAIMSRSARVLMEGQVRVPGDAF
>Mature_395_residues
AHVPQIRIPAVYMRGGTSKGVFFRLDDLPEAARVPGAARDRLLLRVIGSPDPYGKQIDGMGGATSSTSKTVILSKSARPG
HDVDYLFGQVSIDKAFIDWTGNCGNLSAAVGPFAIAAGLVDASRVPADGIATVRIWQANIGKTIVAHVPITGGEVQETGD
FELDGVTFPAAEIQLEFLDPAADEDGAGGAMFPTGNLVDDLEVPGIGTLKATMINAGIPTIFVEAEAIGYTGTELQDAIN
GDCAALARLEAIRGYGALRMGLIGTLDEIATRQHTPKIAFVARPADYVASSGKTVAAKDVDLLVRAMSMGKLHHAMMGTA
AVAIGTAAAIPGTLVSLAAGGGEREAVRFGHPSGTLRVGAQASRASGEWVVTKAIMSRSARVLMEGQVRVPGDAF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=385, Percent_Identity=33.5064935064935, Blast_Score=175, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 40987; Mature: 40856

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHVPQIRIPAVYMRGGTSKGVFFRLDDLPEAARVPGAARDRLLLRVIGSPDPYGKQIDG
CCCCCCEECCEEEEECCCCCCEEEEECCCCHHHHCCCCHHCEEEEEEECCCCCCCCCCCC
MGGATSSTSKTVILSKSARPGHDVDYLFGQVSIDKAFIDWTGNCGNLSAAVGPFAIAAGL
CCCCCCCCCEEEEEECCCCCCCCHHHEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
VDASRVPADGIATVRIWQANIGKTIVAHVPITGGEVQETGDFELDGVTFPAAEIQLEFLD
HCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PAADEDGAGGAMFPTGNLVDDLEVPGIGTLKATMINAGIPTIFVEAEAIGYTGTELQDAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHH
NGDCAALARLEAIRGYGALRMGLIGTLDEIATRQHTPKIAFVARPADYVASSGKTVAAKD
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCEEEHHH
VDLLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVSLAAGGGEREAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCCEEEECCCCCCEEEC
AQASRASGEWVVTKAIMSRSARVLMEGQVRVPGDAF
CCCCCCCCCEEEEHHHHHCCCEEEEECCEECCCCCC
>Mature Secondary Structure 
AHVPQIRIPAVYMRGGTSKGVFFRLDDLPEAARVPGAARDRLLLRVIGSPDPYGKQIDG
CCCCCEECCEEEEECCCCCCEEEEECCCCHHHHCCCCHHCEEEEEEECCCCCCCCCCCC
MGGATSSTSKTVILSKSARPGHDVDYLFGQVSIDKAFIDWTGNCGNLSAAVGPFAIAAGL
CCCCCCCCCEEEEEECCCCCCCCHHHEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
VDASRVPADGIATVRIWQANIGKTIVAHVPITGGEVQETGDFELDGVTFPAAEIQLEFLD
HCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PAADEDGAGGAMFPTGNLVDDLEVPGIGTLKATMINAGIPTIFVEAEAIGYTGTELQDAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHH
NGDCAALARLEAIRGYGALRMGLIGTLDEIATRQHTPKIAFVARPADYVASSGKTVAAKD
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCEEEHHH
VDLLVRAMSMGKLHHAMMGTAAVAIGTAAAIPGTLVSLAAGGGEREAVRFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCCEEEECCCCCCEEEC
AQASRASGEWVVTKAIMSRSARVLMEGQVRVPGDAF
CCCCCCCCCEEEEHHHHHCCCEEEEECCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA