Click here to switch to the map view.

The map label for this gene is ynfL [H]

Identifier: 238025443

GI number: 238025443

Start: 2648625

End: 2649575

Strand: Reverse

Name: ynfL [H]

Synonym: bglu_2g21330

Alternate gene names: 238025443

Gene position: 2649575-2648625 (Counterclockwise)

Preceding gene: 238025446

Following gene: 238025435

Centisome position: 93.71

GC content: 74.34

Gene sequence:

>951_bases
ATGCTCGACCTGCGCCGACTCCGTTACTTCGTCACCGTCGCCGACGAGCTGCATTTCGGCCGCGCGGCGCAGCGCCTGCG
CATCGCGCAGCCGCCGCTCACGCGCCATATCGCGGCGCTCGAGAGCGAGCTCGGGATTCGCCTGCTCGAACGCTCCACCC
GCGCGGTGCAGATCACGCGCGAGGGCGCGCTGTTCCTCGAACGCGCGCGCGCCGTGATCGAGGCGGCCGGCGAGGCCGAG
GCCACCGCGCGCAAGCTCGCGCAGGGCATGATCGGGCGCATCGTGATCGGCTACGCGAGTTCGATCCCGATGTCGGACGC
GTTCTCGAACCTGGTGCGCGATGCGGGCAAGGCGATGCCCGAGATCGAACTGGTGTTTCGCGAGGCGCCGACCACGCACC
AGCGCCAGCAGATCGCCGACGGCGCGCTCGACCTCGGCTTCGGCTGGGCGATGGCTGCGACGCGGCCCGGGCGGACGGAG
CCGGTTGCTTCGGCCGACCAGCCGCTGCGCTCGCGGGTGCTGTCGCGCGAGCCGCTGGTGGCCGCGGTGCCGGCGCACGG
CGAGCAGGCGCGGCGCGGCGCGCTCGCGTTCGAGACGCTGGCGGGCGAGACCTTCGTCACCTTCGTGCCGGAACACGGCT
CCGCGCTGAACGCGGCGCTCGACACGCTGGCCGCGCGGGCCGGCTTCACGCCGCGCTGCGGCGCCACCGCCTCGCAGATC
ACCACGCTGGTGTCACTGGTGGCCGCCGGGCGCGGCATCGCCATCGTGCCGGGCTTCACCGCGGCGCTGCAGCGCCCCGG
GGTGGCCTACGTGCCGCTCGCGGCGGCGCCTCTGATGATCGAACAGACCGTGACGTGGCGCGAGCCCTGCGCGGCCGGTT
GCGTCGCGCGCTTCGTCGAACTCGTGGACGCGGTCGCGCCGGATCGTGCCGGCGGCCCGGCCGGTCGGTGA

Upstream 100 bases:

>100_bases
TGGAAACGCTATGTCCCGTCAGGTCCAGCCGACAGTCTGACGACGCCGCGCGGCGCGCGCCAATACCGTTTTCGTCGCCG
TTGATACCGGAACCACCACC

Downstream 100 bases:

>100_bases
GGCAGCCCCGATGCCCCCTGGGCTTGCTCGTCACGGCCGGCGCCGTCGGCGGCGCCGGCGCGACGAGATCGAGCGGGCTC
GGCCAGCCCGTTTGCCGAAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MLDLRRLRYFVTVADELHFGRAAQRLRIAQPPLTRHIAALESELGIRLLERSTRAVQITREGALFLERARAVIEAAGEAE
ATARKLAQGMIGRIVIGYASSIPMSDAFSNLVRDAGKAMPEIELVFREAPTTHQRQQIADGALDLGFGWAMAATRPGRTE
PVASADQPLRSRVLSREPLVAAVPAHGEQARRGALAFETLAGETFVTFVPEHGSALNAALDTLAARAGFTPRCGATASQI
TTLVSLVAAGRGIAIVPGFTAALQRPGVAYVPLAAAPLMIEQTVTWREPCAAGCVARFVELVDAVAPDRAGGPAGR

Sequences:

>Translated_316_residues
MLDLRRLRYFVTVADELHFGRAAQRLRIAQPPLTRHIAALESELGIRLLERSTRAVQITREGALFLERARAVIEAAGEAE
ATARKLAQGMIGRIVIGYASSIPMSDAFSNLVRDAGKAMPEIELVFREAPTTHQRQQIADGALDLGFGWAMAATRPGRTE
PVASADQPLRSRVLSREPLVAAVPAHGEQARRGALAFETLAGETFVTFVPEHGSALNAALDTLAARAGFTPRCGATASQI
TTLVSLVAAGRGIAIVPGFTAALQRPGVAYVPLAAAPLMIEQTVTWREPCAAGCVARFVELVDAVAPDRAGGPAGR
>Mature_316_residues
MLDLRRLRYFVTVADELHFGRAAQRLRIAQPPLTRHIAALESELGIRLLERSTRAVQITREGALFLERARAVIEAAGEAE
ATARKLAQGMIGRIVIGYASSIPMSDAFSNLVRDAGKAMPEIELVFREAPTTHQRQQIADGALDLGFGWAMAATRPGRTE
PVASADQPLRSRVLSREPLVAAVPAHGEQARRGALAFETLAGETFVTFVPEHGSALNAALDTLAARAGFTPRCGATASQI
TTLVSLVAAGRGIAIVPGFTAALQRPGVAYVPLAAAPLMIEQTVTWREPCAAGCVARFVELVDAVAPDRAGGPAGR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=285, Percent_Identity=35.0877192982456, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI2367136, Length=271, Percent_Identity=31.7343173431734, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1788887, Length=310, Percent_Identity=30, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790399, Length=274, Percent_Identity=29.1970802919708, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788297, Length=258, Percent_Identity=27.1317829457364, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788748, Length=260, Percent_Identity=24.6153846153846, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693105, Length=280, Percent_Identity=24.6428571428571, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=279, Percent_Identity=25.8064516129032, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33542; Mature: 33542

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLRRLRYFVTVADELHFGRAAQRLRIAQPPLTRHIAALESELGIRLLERSTRAVQITR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEH
EGALFLERARAVIEAAGEAEATARKLAQGMIGRIVIGYASSIPMSDAFSNLVRDAGKAMP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
EIELVFREAPTTHQRQQIADGALDLGFGWAMAATRPGRTEPVASADQPLRSRVLSREPLV
CEEEEEECCCCHHHHHHHHCCHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCE
AAVPAHGEQARRGALAFETLAGETFVTFVPEHGSALNAALDTLAARAGFTPRCGATASQI
EECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
TTLVSLVAAGRGIAIVPGFTAALQRPGVAYVPLAAAPLMIEQTVTWREPCAAGCVARFVE
HHHHHHHHCCCCEEEECCHHHHHCCCCCEEECHHHCCHHHHHCCCHHCHHHHHHHHHHHH
LVDAVAPDRAGGPAGR
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLDLRRLRYFVTVADELHFGRAAQRLRIAQPPLTRHIAALESELGIRLLERSTRAVQITR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEH
EGALFLERARAVIEAAGEAEATARKLAQGMIGRIVIGYASSIPMSDAFSNLVRDAGKAMP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
EIELVFREAPTTHQRQQIADGALDLGFGWAMAATRPGRTEPVASADQPLRSRVLSREPLV
CEEEEEECCCCHHHHHHHHCCHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCE
AAVPAHGEQARRGALAFETLAGETFVTFVPEHGSALNAALDTLAARAGFTPRCGATASQI
EECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
TTLVSLVAAGRGIAIVPGFTAALQRPGVAYVPLAAAPLMIEQTVTWREPCAAGCVARFVE
HHHHHHHHCCCCEEEECCHHHHHCCCCCEEECHHHCCHHHHHCCCHHCHHHHHHHHHHHH
LVDAVAPDRAGGPAGR
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]