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The map label for this gene is hipO [H]

Identifier: 238025402

GI number: 238025402

Start: 2582225

End: 2583412

Strand: Reverse

Name: hipO [H]

Synonym: bglu_2g20890

Alternate gene names: 238025402

Gene position: 2583412-2582225 (Counterclockwise)

Preceding gene: 238025403

Following gene: 238025401

Centisome position: 91.37

GC content: 69.53

Gene sequence:

>1188_bases
ATGACCGACACCCGCCACACCGAACTCGCCGACCTCGCCGGCGACACCTCCGAGCTGCTCGAGATCCGCCACCATCTGCA
TCGCCACCCCGAACTCGCGTTCGAGGAGGTGGCCACGGCCGCGCTGGTCGCCGAGCGGCTCGAGAGCTGGGGCTGGCAGG
TCACGCGCGGAGTGGGCGAGACGGGCGTGGTCGGCACGCTGACGGTGGACGGCGGCACGCGCCGGATCGGCATCCGCGCC
GACATGGACGCCTTGCCGATCGGCGAGGAAACCGGCCTGCCCTACGCGAGCGCGACACCCGGCAAGATGCATGCGTGCGG
CCACGACGGCCACACCGCCATGCTGCTCGGCGCGGCGCGTCAGCTCGCGCGCACGCGGCGCTTCTCGGGCACGGTCCACC
TTTATTTCCAGCCGGCCGAGGAAAAGGGCGTGGACAGCGGCGCGCAGCGCATGATCGCCGACGGCCTGTTCGAGCGCTTT
CCGTGCGACGCGGTGTTCGGCATGCACAACCACCCGGGCGCGGAGCCGGGCAAGCTGCTGTTCCGGCGCGGCCCGTTCAT
GGCGGCGGGCGACCGCGTCTGGATCGAGATCGAGGGCGTGGGCGGCCACGCGGCGCGCCCGCACCTGACGGTGGACGTGG
TGGTGGTGGCGGCCAGCATCGTGATGGCGCGGCAGACCGTGGTCTCGCGCAACCTCGACCCCGCGCAGCAGGCGGTGGTG
ACGATCGGCTCGATCCACGCGGGCAGTGCGAACAACGTGATTCCGGAGCGCGCGCAACTCGCGCTGAGCGTGCGCTCGTT
CAGCGAGGCGGTCCGCGCGCAACTGAAGCGCCGCATCATCGAGATCGCGCAAGGCCAGGCAGCCAGCTACGGCGCCAAGG
CCCACGTCGAATACACCGAGGGCTACCCGGTGGTGGTCAATTCGGACGCCGAAACCGATTTTGCCGCGCAGGTGGCCAAG
GAACTGGTGGGCGAGCACAACGTGCTCGAGCAGATCGATCCGTTGATGGGCAGCGAGGATTTCGCCTACATGCTGCAGCA
GCGTCCCGGCTCGTTCGTGCGAATCGGCAACGGCGTGGGCGAGGACGGCTGCATGGTCCACAATCCGCACTACGATTTCA
ACGATCACAACCTGCCGATCGGCGCCGCGTTCTGGGCGCGGCTGGTGGAGCGCTATCTGTCGCGCTGA

Upstream 100 bases:

>100_bases
TTGGCTATAGTGAGGTGGTTTGCCGGCGCATCCGCCGCCGCGCTTTTCCCACCTTCGCGCCGGGCCCCTTGCGCCCGCGC
CAGGGTTCCCCGGAGCCACG

Downstream 100 bases:

>100_bases
CGGTCCGGCGCGTGGCGGCCCCGATTCGGCACCCGGACGGCTTCGCTCCAAGCTCGGGGGCTGGCGCGCGGCAATCGTTG
CGCACGCATCGATTGCCGCG

Product: Amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTDTRHTELADLAGDTSELLEIRHHLHRHPELAFEEVATAALVAERLESWGWQVTRGVGETGVVGTLTVDGGTRRIGIRA
DMDALPIGEETGLPYASATPGKMHACGHDGHTAMLLGAARQLARTRRFSGTVHLYFQPAEEKGVDSGAQRMIADGLFERF
PCDAVFGMHNHPGAEPGKLLFRRGPFMAAGDRVWIEIEGVGGHAARPHLTVDVVVVAASIVMARQTVVSRNLDPAQQAVV
TIGSIHAGSANNVIPERAQLALSVRSFSEAVRAQLKRRIIEIAQGQAASYGAKAHVEYTEGYPVVVNSDAETDFAAQVAK
ELVGEHNVLEQIDPLMGSEDFAYMLQQRPGSFVRIGNGVGEDGCMVHNPHYDFNDHNLPIGAAFWARLVERYLSR

Sequences:

>Translated_395_residues
MTDTRHTELADLAGDTSELLEIRHHLHRHPELAFEEVATAALVAERLESWGWQVTRGVGETGVVGTLTVDGGTRRIGIRA
DMDALPIGEETGLPYASATPGKMHACGHDGHTAMLLGAARQLARTRRFSGTVHLYFQPAEEKGVDSGAQRMIADGLFERF
PCDAVFGMHNHPGAEPGKLLFRRGPFMAAGDRVWIEIEGVGGHAARPHLTVDVVVVAASIVMARQTVVSRNLDPAQQAVV
TIGSIHAGSANNVIPERAQLALSVRSFSEAVRAQLKRRIIEIAQGQAASYGAKAHVEYTEGYPVVVNSDAETDFAAQVAK
ELVGEHNVLEQIDPLMGSEDFAYMLQQRPGSFVRIGNGVGEDGCMVHNPHYDFNDHNLPIGAAFWARLVERYLSR
>Mature_394_residues
TDTRHTELADLAGDTSELLEIRHHLHRHPELAFEEVATAALVAERLESWGWQVTRGVGETGVVGTLTVDGGTRRIGIRAD
MDALPIGEETGLPYASATPGKMHACGHDGHTAMLLGAARQLARTRRFSGTVHLYFQPAEEKGVDSGAQRMIADGLFERFP
CDAVFGMHNHPGAEPGKLLFRRGPFMAAGDRVWIEIEGVGGHAARPHLTVDVVVVAASIVMARQTVVSRNLDPAQQAVVT
IGSIHAGSANNVIPERAQLALSVRSFSEAVRAQLKRRIIEIAQGQAASYGAKAHVEYTEGYPVVVNSDAETDFAAQVAKE
LVGEHNVLEQIDPLMGSEDFAYMLQQRPGSFVRIGNGVGEDGCMVHNPHYDFNDHNLPIGAAFWARLVERYLSR

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=423, Percent_Identity=27.4231678486998, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787598, Length=288, Percent_Identity=26.3888888888889, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42857; Mature: 42726

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTRHTELADLAGDTSELLEIRHHLHRHPELAFEEVATAALVAERLESWGWQVTRGVGE
CCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCC
TGVVGTLTVDGGTRRIGIRADMDALPIGEETGLPYASATPGKMHACGHDGHTAMLLGAAR
CCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHH
QLARTRRFSGTVHLYFQPAEEKGVDSGAQRMIADGLFERFPCDAVFGMHNHPGAEPGKLL
HHHHHHHCCCEEEEEEECCHHCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHH
FRRGPFMAAGDRVWIEIEGVGGHAARPHLTVDVVVVAASIVMARQTVVSRNLDPAQQAVV
HCCCCCEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHE
TIGSIHAGSANNVIPERAQLALSVRSFSEAVRAQLKRRIIEIAQGQAASYGAKAHVEYTE
EHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
GYPVVVNSDAETDFAAQVAKELVGEHNVLEQIDPLMGSEDFAYMLQQRPGSFVRIGNGVG
CCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCC
EDGCMVHNPHYDFNDHNLPIGAAFWARLVERYLSR
CCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDTRHTELADLAGDTSELLEIRHHLHRHPELAFEEVATAALVAERLESWGWQVTRGVGE
CCCCCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCC
TGVVGTLTVDGGTRRIGIRADMDALPIGEETGLPYASATPGKMHACGHDGHTAMLLGAAR
CCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHH
QLARTRRFSGTVHLYFQPAEEKGVDSGAQRMIADGLFERFPCDAVFGMHNHPGAEPGKLL
HHHHHHHCCCEEEEEEECCHHCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCHHHHH
FRRGPFMAAGDRVWIEIEGVGGHAARPHLTVDVVVVAASIVMARQTVVSRNLDPAQQAVV
HCCCCCEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHE
TIGSIHAGSANNVIPERAQLALSVRSFSEAVRAQLKRRIIEIAQGQAASYGAKAHVEYTE
EHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
GYPVVVNSDAETDFAAQVAKELVGEHNVLEQIDPLMGSEDFAYMLQQRPGSFVRIGNGVG
CCCEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCCC
EDGCMVHNPHYDFNDHNLPIGAAFWARLVERYLSR
CCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]