Click here to switch to the map view.

The map label for this gene is thcR [H]

Identifier: 238025382

GI number: 238025382

Start: 2556142

End: 2557176

Strand: Reverse

Name: thcR [H]

Synonym: bglu_2g20640

Alternate gene names: 238025382

Gene position: 2557176-2556142 (Counterclockwise)

Preceding gene: 238025389

Following gene: 238025381

Centisome position: 90.44

GC content: 65.02

Gene sequence:

>1035_bases
ATGGCGCATTGTTTAGCTGGGAACCGCCTTGCCGACGCCCCATGGTTCAACTGCGGCAATCTGTTGATGCAATCGACGGA
CCTCGACGAGGTGCGGGCGTCGGTGGCCGACGTGCTCAAGCCGCATCGGCTGACCGTCGCCGGCGGCGCGAGCGCGCTGC
ATGGCCGTTTGCATCACACGCGATGGGGCAACCTTTCGCTGAATCTGCTCGACTACGGCAGCGAAGTCATCATCGAACCC
GATCGGCTCGAGGATTTCTTCCTGCTTCAAGTGCCGTTGCAGGGGCAGGCGGAAATCGAATGCGGCGGCCAGCGGTTCGT
CTCGTCGCCTTCCACCGCTTCGCTGGTCTCGCCGTCGCTGGCGCTGCGCATGCGCTGGGGCGAAGCGTGCCCTCAGGCCA
TTCTGCGCATCGAGCGCTCGGCCATGGAGCGCCACGCCCAGCGCCACTTCGGTGAAGCGGCGCGCGGGCCGATCGAGTTC
GCACCGGAATTCGACCTCACGTCGCCGCAGGGGCAGTGCCTCATGCAACTGCTTCCGGTACTGGCGGAAATCATGAGCAC
GGAGGCGCATCCGCTGCGCCACGCGCTCGCCTTCGAGCAGTTCGAATCGACACTGCTCAATGCGCTGATCTACGGCCAAC
CGAACAGCGCGCGCAACGACAAGGCGCGAGAGCGCCGGCCGCTGGCGCCGACTTGCGTGCGGCGTGTCGAGGAATATATC
CGCACCCATTTGCAGGAGCCGCTGACCATCGAGCAGCTTGCCGAATTCGGCAACGTCAGTGCGAGCACCTTGTTTGCGGC
CTTCAAGCATTATCACGGCGTGACGCCGATGGCTTTCGTGCGCCAGTTGCGGCTCGAGCGCGTGCGTCGCGACCTGATCG
GCGCGGATGCCATGCACGGCACGGGGCGCCCGTCGGTGACGGATATCGCGCTGAAATGGGGTTTCGCCCATCTCGGGCGT
TTCGCGATGGAATACAAGCGCGCCTTTGGCGAATCGCCTTCGGCGAGCCTGAGGATGCGCCGCGAGATGCGCTAG

Upstream 100 bases:

>100_bases
GCGTCGCTATCCGAATCCTCCGGGAATCCGGCCGTGCGGATCACCAAGACGGTGATAGATGCCCTCATGAAGTGCAGATC
AGCGTGATGGGAGAACCTCG

Downstream 100 bases:

>100_bases
GCCGCCAGGCGCGTGTGACCCGGGGGAGCGGCGTGCGACGCTGCTAGCGGCTTCAACGACCCCAGGAGCCCCGCGCGGCG
CAACGCACGGCGCACGCATC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MAHCLAGNRLADAPWFNCGNLLMQSTDLDEVRASVADVLKPHRLTVAGGASALHGRLHHTRWGNLSLNLLDYGSEVIIEP
DRLEDFFLLQVPLQGQAEIECGGQRFVSSPSTASLVSPSLALRMRWGEACPQAILRIERSAMERHAQRHFGEAARGPIEF
APEFDLTSPQGQCLMQLLPVLAEIMSTEAHPLRHALAFEQFESTLLNALIYGQPNSARNDKARERRPLAPTCVRRVEEYI
RTHLQEPLTIEQLAEFGNVSASTLFAAFKHYHGVTPMAFVRQLRLERVRRDLIGADAMHGTGRPSVTDIALKWGFAHLGR
FAMEYKRAFGESPSASLRMRREMR

Sequences:

>Translated_344_residues
MAHCLAGNRLADAPWFNCGNLLMQSTDLDEVRASVADVLKPHRLTVAGGASALHGRLHHTRWGNLSLNLLDYGSEVIIEP
DRLEDFFLLQVPLQGQAEIECGGQRFVSSPSTASLVSPSLALRMRWGEACPQAILRIERSAMERHAQRHFGEAARGPIEF
APEFDLTSPQGQCLMQLLPVLAEIMSTEAHPLRHALAFEQFESTLLNALIYGQPNSARNDKARERRPLAPTCVRRVEEYI
RTHLQEPLTIEQLAEFGNVSASTLFAAFKHYHGVTPMAFVRQLRLERVRRDLIGADAMHGTGRPSVTDIALKWGFAHLGR
FAMEYKRAFGESPSASLRMRREMR
>Mature_343_residues
AHCLAGNRLADAPWFNCGNLLMQSTDLDEVRASVADVLKPHRLTVAGGASALHGRLHHTRWGNLSLNLLDYGSEVIIEPD
RLEDFFLLQVPLQGQAEIECGGQRFVSSPSTASLVSPSLALRMRWGEACPQAILRIERSAMERHAQRHFGEAARGPIEFA
PEFDLTSPQGQCLMQLLPVLAEIMSTEAHPLRHALAFEQFESTLLNALIYGQPNSARNDKARERRPLAPTCVRRVEEYIR
THLQEPLTIEQLAEFGNVSASTLFAAFKHYHGVTPMAFVRQLRLERVRRDLIGADAMHGTGRPSVTDIALKWGFAHLGRF
AMEYKRAFGESPSASLRMRREMR

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=108, Percent_Identity=38.8888888888889, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 38512; Mature: 38380

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHCLAGNRLADAPWFNCGNLLMQSTDLDEVRASVADVLKPHRLTVAGGASALHGRLHHT
CCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCC
RWGNLSLNLLDYGSEVIIEPDRLEDFFLLQVPLQGQAEIECGGQRFVSSPSTASLVSPSL
CCCCEEEEEEECCCEEEECCCCCCCEEEEEECCCCCCEEECCCHHHCCCCCHHHHHCHHH
ALRMRWGEACPQAILRIERSAMERHAQRHFGEAARGPIEFAPEFDLTSPQGQCLMQLLPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LAEIMSTEAHPLRHALAFEQFESTLLNALIYGQPNSARNDKARERRPLAPTCVRRVEEYI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
RTHLQEPLTIEQLAEFGNVSASTLFAAFKHYHGVTPMAFVRQLRLERVRRDLIGADAMHG
HHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
TGRPSVTDIALKWGFAHLGRFAMEYKRAFGESPSASLRMRREMR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AHCLAGNRLADAPWFNCGNLLMQSTDLDEVRASVADVLKPHRLTVAGGASALHGRLHHT
CCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCC
RWGNLSLNLLDYGSEVIIEPDRLEDFFLLQVPLQGQAEIECGGQRFVSSPSTASLVSPSL
CCCCEEEEEEECCCEEEECCCCCCCEEEEEECCCCCCEEECCCHHHCCCCCHHHHHCHHH
ALRMRWGEACPQAILRIERSAMERHAQRHFGEAARGPIEFAPEFDLTSPQGQCLMQLLPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
LAEIMSTEAHPLRHALAFEQFESTLLNALIYGQPNSARNDKARERRPLAPTCVRRVEEYI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
RTHLQEPLTIEQLAEFGNVSASTLFAAFKHYHGVTPMAFVRQLRLERVRRDLIGADAMHG
HHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
TGRPSVTDIALKWGFAHLGRFAMEYKRAFGESPSASLRMRREMR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]