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The map label for this gene is pleD [H]

Identifier: 238025376

GI number: 238025376

Start: 2541635

End: 2542405

Strand: Reverse

Name: pleD [H]

Synonym: bglu_2g20540

Alternate gene names: 238025376

Gene position: 2542405-2541635 (Counterclockwise)

Preceding gene: 238025377

Following gene: 238025375

Centisome position: 89.92

GC content: 69.0

Gene sequence:

>771_bases
GTGTCCGCCCCAACCATCGAACTCGTCGATCAGGCCTGCCGTGCGGCCGGCTTCACCACGTGGCAGGAACTGGTGGCAGC
GGCGGGCGGCGGGGCGCTGTTCAAGCTGAGCTTCGCAGAAAGGCGGCCGGCGAACTTCGTGAAGGAGTTTGCTGCGCTGA
TCAAGTGGCATGGCCTCGGACTATGCGCCGACAATGCGCTGGACCTCTACCTTGGCATGCCTGCCATCCTGAAGCCGGTG
ATCGATACCGGCAGGAGCGCGCAGGCCCTCAGGCTGCCCGAGCTGGGCAAGCTGCAGGAGACGCAGGCGGGCCTCGTGGC
GCTCTCGGGGGTACGACGTTCGGCCGGCGTCAAGTACGAGCGGCGCGCGACATCGCCGGAACTCGAAGGCGTCAGGGTGC
TCGTCCTCGACGACGACGAGGTATCGTGCAAGGTCATCTCGGCGATGCTGAAACGCGCCGGCTCGCAGGTATCGGCATGC
GCGGAGGCCGAGGCCGCGCTGGCCCTGCTGAAGCAGGCGCCGCCCGACATCGTCGTGCTCGACGTGGTGCTGGGCGGCGC
GCTCGACGGCTTCGAGTTCTGTCGTGTGATGCGTACCAACGAGCGCTGCGCGCAGGTGCCCGCGATCTTCGTCACCGGTC
AGGCCGGCCCCCATTCGCGCGCGCGGGCCGACGACGTGCGCGCGTCGGCATTTCTGGAAAAACCCCTGCGGGGCGACCAG
CTGCGTGCCGCGGTGAACGGGCTGGCCCGGCACCTGCAGCCGGCCTGCTGA

Upstream 100 bases:

>100_bases
GGCGCAGGCCCGCCCATGTGGGCAAACGATCTAACTTGAACAGGGGGACCAGATGCCGAGAAGAAAAGCGAATCAAGCGA
CGCCGGGGGAGTTTCCCCAG

Downstream 100 bases:

>100_bases
GGCGCGGCCGCGGCCGCACGGCGCTGGCCGCGGCCCGGGCGAATGTCGCTCTCGCCGCCGTGCGAAGCCCGCGTCGAGCC
GGACTCCCTCGTCAAGCACA

Product: adenylate/guanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSAPTIELVDQACRAAGFTTWQELVAAAGGGALFKLSFAERRPANFVKEFAALIKWHGLGLCADNALDLYLGMPAILKPV
IDTGRSAQALRLPELGKLQETQAGLVALSGVRRSAGVKYERRATSPELEGVRVLVLDDDEVSCKVISAMLKRAGSQVSAC
AEAEAALALLKQAPPDIVVLDVVLGGALDGFEFCRVMRTNERCAQVPAIFVTGQAGPHSRARADDVRASAFLEKPLRGDQ
LRAAVNGLARHLQPAC

Sequences:

>Translated_256_residues
MSAPTIELVDQACRAAGFTTWQELVAAAGGGALFKLSFAERRPANFVKEFAALIKWHGLGLCADNALDLYLGMPAILKPV
IDTGRSAQALRLPELGKLQETQAGLVALSGVRRSAGVKYERRATSPELEGVRVLVLDDDEVSCKVISAMLKRAGSQVSAC
AEAEAALALLKQAPPDIVVLDVVLGGALDGFEFCRVMRTNERCAQVPAIFVTGQAGPHSRARADDVRASAFLEKPLRGDQ
LRAAVNGLARHLQPAC
>Mature_255_residues
SAPTIELVDQACRAAGFTTWQELVAAAGGGALFKLSFAERRPANFVKEFAALIKWHGLGLCADNALDLYLGMPAILKPVI
DTGRSAQALRLPELGKLQETQAGLVALSGVRRSAGVKYERRATSPELEGVRVLVLDDDEVSCKVISAMLKRAGSQVSACA
EAEAALALLKQAPPDIVVLDVVLGGALDGFEFCRVMRTNERCAQVPAIFVTGQAGPHSRARADDVRASAFLEKPLRGDQL
RAAVNGLARHLQPAC

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 27201; Mature: 27070

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPTIELVDQACRAAGFTTWQELVAAAGGGALFKLSFAERRPANFVKEFAALIKWHGLG
CCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCC
LCADNALDLYLGMPAILKPVIDTGRSAQALRLPELGKLQETQAGLVALSGVRRSAGVKYE
EECCCCHHHHHCCHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
RRATSPELEGVRVLVLDDDEVSCKVISAMLKRAGSQVSACAEAEAALALLKQAPPDIVVL
HCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEE
DVVLGGALDGFEFCRVMRTNERCAQVPAIFVTGQAGPHSRARADDVRASAFLEKPLRGDQ
EEHHCCCCCHHHHHHHHHCCHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHH
LRAAVNGLARHLQPAC
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAPTIELVDQACRAAGFTTWQELVAAAGGGALFKLSFAERRPANFVKEFAALIKWHGLG
CCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCC
LCADNALDLYLGMPAILKPVIDTGRSAQALRLPELGKLQETQAGLVALSGVRRSAGVKYE
EECCCCHHHHHCCHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
RRATSPELEGVRVLVLDDDEVSCKVISAMLKRAGSQVSACAEAEAALALLKQAPPDIVVL
HCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEE
DVVLGGALDGFEFCRVMRTNERCAQVPAIFVTGQAGPHSRARADDVRASAFLEKPLRGDQ
EEHHCCCCCHHHHHHHHHCCHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHH
LRAAVNGLARHLQPAC
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA