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The map label for this gene is ansA [H]

Identifier: 238025155

GI number: 238025155

Start: 2275129

End: 2276277

Strand: Reverse

Name: ansA [H]

Synonym: bglu_2g18200

Alternate gene names: 238025155

Gene position: 2276277-2275129 (Counterclockwise)

Preceding gene: 238025156

Following gene: 238025154

Centisome position: 80.51

GC content: 69.8

Gene sequence:

>1149_bases
ATGAACAATATTTCCCATGTCGGGAAAACCGGGTCTCGCCATGCCCTGCTCCGTCTCGCCGCTGCCGCGGCGCTGACCGC
CTTCGGCGCGCTCGGCGCGCCGGCCTCGTTCGCCGCCGCCGCCGCGGCACCCGTGGTGGCGGACGCGCAGGCCGCCGTGT
CGCTGCCGCGCGTCGCGATCCTCGCCACCGGCGGCACCATCGCGGGCAAGGCCGCCGCGCGCGCCGCGAACGCCTACGAC
GCCGGCGCGCTCGGCGTGCGGCAATTGGTGGACGCCGTGCCCGGCATCGAGAAGCTCGCGCGCCTGCGCCCGGAACAGAT
CGCCTCGATCGGCTCGCAGGACATGAACGACGCGGTCTGGTTCAAGCTCGCCAAGCGCATCCAGGACATCTCCGATCGCA
ACGAGGCCGACGCGATCGTGATCACGCACGGCACCGATACGCTCGAGGAAACCGCGTTCTTCCTCGACAACGTCCTGCAC
ACCGACAAGCCGGTCGTGCTGGTCGGATCGATGCGGCCGTCCACGGCGATCAGCGCCGACGGGCCCGCCAACCTCTACGA
GGCGGTGGAAGTGGCGGCCGATCCGCATGCGCGCGGGCGCGGCGTGATGATCGTGCTGAACGACACGATCCATTCGGCGC
GCGGCGCGACCAAGACCAATTCCACCAGTGTGCAGACCTTCGCCTCGCCGAACAGCGGCCCGATCGGCTACGTCGATCCG
GCGAGCGTGCGCTTCCTCGCGCCGGCCGCGGCCGATTCCCGCAAGAAGTACGTGCTGCCCGCCGACGGCAAGCTGCCGGA
CGTCGAGATCGTCTATTCGCACAGCAACATGGATGCGCGGCAGATCGACCACGCGATCGCCGACCACGTGAAGGGCATCG
TGCTGGCCGGGGTCGGCGACGGCAATACCTCCGGCAAGGCGCTCGACGCGCTGCAGCAAGCGGCGCGGCAGGGCATCGTC
GTGGTGCGCTCGACGCGCGTCGGCAGCGGCTTCGTCAACCGGAACGTGGAAGTGAACGACGACCAGCGCGGCTTTGCGGT
GTCGCTCGACCTGAACCCGCAGAAGGCGCGGATACTCACCCAGTTGCTGGTCGCGAACCGCGTGACCTCGCCGGCCGAGG
TCCAGCGGGCGTTCTCGGCGACCTGGTAG

Upstream 100 bases:

>100_bases
CTCGATGCGCCCAGGGCCGGCTGCGGGCCGATCGGCAGTGACTGCGCCGGATGATGTTTGCGAAGTGCAATTCATCGAAT
CAAAAAGTAGGGAGTCTTGA

Downstream 100 bases:

>100_bases
GGCACCGGCTCGCCGCCCGCGCCGGTCGGGCCGTTGGCCCTCGCGCGGCGGCGCCCGTGACGGTCCGGGCGGCGCCGCGC
CGGCTGCCGGGCTTTGTCAG

Product: putative L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNNISHVGKTGSRHALLRLAAAAALTAFGALGAPASFAAAAAAPVVADAQAAVSLPRVAILATGGTIAGKAAARAANAYD
AGALGVRQLVDAVPGIEKLARLRPEQIASIGSQDMNDAVWFKLAKRIQDISDRNEADAIVITHGTDTLEETAFFLDNVLH
TDKPVVLVGSMRPSTAISADGPANLYEAVEVAADPHARGRGVMIVLNDTIHSARGATKTNSTSVQTFASPNSGPIGYVDP
ASVRFLAPAAADSRKKYVLPADGKLPDVEIVYSHSNMDARQIDHAIADHVKGIVLAGVGDGNTSGKALDALQQAARQGIV
VVRSTRVGSGFVNRNVEVNDDQRGFAVSLDLNPQKARILTQLLVANRVTSPAEVQRAFSATW

Sequences:

>Translated_382_residues
MNNISHVGKTGSRHALLRLAAAAALTAFGALGAPASFAAAAAAPVVADAQAAVSLPRVAILATGGTIAGKAAARAANAYD
AGALGVRQLVDAVPGIEKLARLRPEQIASIGSQDMNDAVWFKLAKRIQDISDRNEADAIVITHGTDTLEETAFFLDNVLH
TDKPVVLVGSMRPSTAISADGPANLYEAVEVAADPHARGRGVMIVLNDTIHSARGATKTNSTSVQTFASPNSGPIGYVDP
ASVRFLAPAAADSRKKYVLPADGKLPDVEIVYSHSNMDARQIDHAIADHVKGIVLAGVGDGNTSGKALDALQQAARQGIV
VVRSTRVGSGFVNRNVEVNDDQRGFAVSLDLNPQKARILTQLLVANRVTSPAEVQRAFSATW
>Mature_382_residues
MNNISHVGKTGSRHALLRLAAAAALTAFGALGAPASFAAAAAAPVVADAQAAVSLPRVAILATGGTIAGKAAARAANAYD
AGALGVRQLVDAVPGIEKLARLRPEQIASIGSQDMNDAVWFKLAKRIQDISDRNEADAIVITHGTDTLEETAFFLDNVLH
TDKPVVLVGSMRPSTAISADGPANLYEAVEVAADPHARGRGVMIVLNDTIHSARGATKTNSTSVQTFASPNSGPIGYVDP
ASVRFLAPAAADSRKKYVLPADGKLPDVEIVYSHSNMDARQIDHAIADHVKGIVLAGVGDGNTSGKALDALQQAARQGIV
VVRSTRVGSGFVNRNVEVNDDQRGFAVSLDLNPQKARILTQLLVANRVTSPAEVQRAFSATW

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=342, Percent_Identity=46.1988304093567, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1788065, Length=235, Percent_Identity=29.7872340425532, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320527, Length=336, Percent_Identity=38.0952380952381, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6323189, Length=335, Percent_Identity=38.5074626865672, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323187, Length=335, Percent_Identity=38.5074626865672, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323186, Length=335, Percent_Identity=38.5074626865672, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323184, Length=335, Percent_Identity=38.5074626865672, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24645530, Length=237, Percent_Identity=26.1603375527426, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 39721; Mature: 39721

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNISHVGKTGSRHALLRLAAAAALTAFGALGAPASFAAAAAAPVVADAQAAVSLPRVAI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHCCHHHHCCCEEEE
LATGGTIAGKAAARAANAYDAGALGVRQLVDAVPGIEKLARLRPEQIASIGSQDMNDAVW
EECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHCCHHHHHHCCCCCCHHHHH
FKLAKRIQDISDRNEADAIVITHGTDTLEETAFFLDNVLHTDKPVVLVGSMRPSTAISAD
HHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEECCC
GPANLYEAVEVAADPHARGRGVMIVLNDTIHSARGATKTNSTSVQTFASPNSGPIGYVDP
CCHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHCCCCCCCCCCEEEEECCCCCCCCCCCC
ASVRFLAPAAADSRKKYVLPADGKLPDVEIVYSHSNMDARQIDHAIADHVKGIVLAGVGD
CCEEEEECCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCC
GNTSGKALDALQQAARQGIVVVRSTRVGSGFVNRNVEVNDDQRGFAVSLDLNPQKARILT
CCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHCCCEEECCCCCCEEEEEECCHHHHHHHH
QLLVANRVTSPAEVQRAFSATW
HHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNNISHVGKTGSRHALLRLAAAAALTAFGALGAPASFAAAAAAPVVADAQAAVSLPRVAI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHCCHHHHCCCEEEE
LATGGTIAGKAAARAANAYDAGALGVRQLVDAVPGIEKLARLRPEQIASIGSQDMNDAVW
EECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHCCHHHHHHCCCCCCHHHHH
FKLAKRIQDISDRNEADAIVITHGTDTLEETAFFLDNVLHTDKPVVLVGSMRPSTAISAD
HHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEECCC
GPANLYEAVEVAADPHARGRGVMIVLNDTIHSARGATKTNSTSVQTFASPNSGPIGYVDP
CCHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHCCCCCCCCCCEEEEECCCCCCCCCCCC
ASVRFLAPAAADSRKKYVLPADGKLPDVEIVYSHSNMDARQIDHAIADHVKGIVLAGVGD
CCEEEEECCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCC
GNTSGKALDALQQAARQGIVVVRSTRVGSGFVNRNVEVNDDQRGFAVSLDLNPQKARILT
CCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHCCCEEECCCCCCEEEEEECCHHHHHHHH
QLLVANRVTSPAEVQRAFSATW
HHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]