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The map label for this gene is budB [H]

Identifier: 238025045

GI number: 238025045

Start: 2134165

End: 2135811

Strand: Reverse

Name: budB [H]

Synonym: bglu_2g17020

Alternate gene names: 238025045

Gene position: 2135811-2134165 (Counterclockwise)

Preceding gene: 238025046

Following gene: 238025044

Centisome position: 75.54

GC content: 66.61

Gene sequence:

>1647_bases
ATGAAGGCATCCGATCTGTTCGTCAAGGCACTGGAAGCGGAGGGCGTCGAATACGTATTCGGCATCCCCGGCGAAGAAAA
TCTCGATCTGCTCGAATCGATTCGGCACTCGCGCATCAAGCTGGTGCTGACGCGCCACGAGCAGGCGGCGGGCTTCATGG
CCGCCACCTACGGGCGGCTCACCGGCAAGGCCGGCGTGTGCCTGTCCACGCTCGGCCCGGGCGCCACCAACCTCGTCACG
GCCGCCGCCTACGCGCAGCTGGGCGGCATGCCGATGCTGATGGTCACCGGCCAGAAGCCGATCAAGACCAGCAAGCAGGG
CCATTTCCAGATCGTCGACGTGGTCGACATGATGCAGCCGCTCACCAAGCTCACGCGCCAGATCGTGTCGATCGGCCATA
TCCCGTCGGTGGTGCGCGAGGCGTTCCGGCGCGCCGAGGACGAGCGCCCCGGCGCGACCCACCTCGAACTGCCCGAGGAC
ATCGCGCACGAGGAGGGCGACGGCAAGCCGATCCCGGCCAGCTACAGCCGCCGCCCGGTGGCCGAGCAAAAGGCGGTGGC
GCGCGCGGTGGCGGCGATCCGCCAGGCACGCCATCCGCTCCTGATGATCGGCGCGGGCGGCAACCGCAAGACCTGCTGCA
AGATGCTGCGCGAATTCGTCGACGCCACCGGCATCCCGTTCTTCACGACGCAGATGGGCAAGGGCGTGATCGACGAATCG
CATCCGCTCTGGCTCGGCAACGCCACGCTGTCGGACGGCGACTTCGTGCATCGCGCGATCGACCATGCCGACTGCATCAT
CAACGTCGGCCACGACGTGATCGAGAAGCCGCCGTTCTTCATGCGCGGCGGCGACCGCACGGTGATTCACGTCAATTTTC
TCGGCGCGCAGGTCGATCCGGTCTATTTCCCGCAGATCGAGGTGGTGGGCGACATCGCCAACGCGGTCTGGCAGATCAAG
GAGGTGCTGGCCGAGCCGCCGCAATGGGACTTCTCGCGCTTCATGCTGATCAAGGCGCGCTTCGACGCGCACCTGAGAAA
GGGCGAGGACGATCCGCGCTACCCGCTGCATCCGGTGCGGGTGGTGCACGACCTGTACGGCGCGCTGCCTGCGGACGGCA
TCGCCTGCCTCGACAACGGCATGTACAAGATCTGGTTCGCGCGCTACTGGCGCGCCCACGAGCCGAACTCGCTGCTGCTC
GACAACGCGCTCGCCTCGATGGGCGCCGGGCTGCCCTCGGCGATCGCCACCAAGATCGTTCATCCGCACCGGAAGGTGGT
GGCGATCTGCGGCGACGGCGGCTTCATGATGAATTCGCAGGAGCTGGAGACGGCGGTGCGCCTGAAGCTCGACCTGCTGG
TGCTGGTGCTGGTGCTGCGCGACGACGCGTTCGGCATGATCCGCTGGAAGCAGGAGAACATGAACTTCCCCGACTATGCG
ATGACGCTCGACAATCCCGACTTCGTCGCCTATGCGCAAAGCTACGGCGCGCAGGGCCATCGCGCGGAATCGGCCGACGC
GCTCGCGCCCTTGCTCGCGCACTGCCTCGCGAGCCCGGGCGTGCATCTGGTCGACGTGCCGATCGACTACTCGGACAACG
AGAAGATCCTGAACCGCGAGATCCCGCGCCTGTCCGCGCAGCTCTGA

Upstream 100 bases:

>100_bases
CAATCTGCAGGTAACGAGCCGCCCGCGTCGTAGGCCGACGCGGGTGTCTGCCACGCCGTGCGCCGGAAGCCGGCACGCGG
CATTCACCGAGAGCGTCCCC

Downstream 100 bases:

>100_bases
ACGGCCGCCGCGCCCTTGTTCCCCACCGTCTTTACCGCACGGAGGCATCCCATGCTGAAGGAATCCTATCCGTACTACCT
CGCCAACGAGGCCGTGTACG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MKASDLFVKALEAEGVEYVFGIPGEENLDLLESIRHSRIKLVLTRHEQAAGFMAATYGRLTGKAGVCLSTLGPGATNLVT
AAAYAQLGGMPMLMVTGQKPIKTSKQGHFQIVDVVDMMQPLTKLTRQIVSIGHIPSVVREAFRRAEDERPGATHLELPED
IAHEEGDGKPIPASYSRRPVAEQKAVARAVAAIRQARHPLLMIGAGGNRKTCCKMLREFVDATGIPFFTTQMGKGVIDES
HPLWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRGGDRTVIHVNFLGAQVDPVYFPQIEVVGDIANAVWQIK
EVLAEPPQWDFSRFMLIKARFDAHLRKGEDDPRYPLHPVRVVHDLYGALPADGIACLDNGMYKIWFARYWRAHEPNSLLL
DNALASMGAGLPSAIATKIVHPHRKVVAICGDGGFMMNSQELETAVRLKLDLLVLVLVLRDDAFGMIRWKQENMNFPDYA
MTLDNPDFVAYAQSYGAQGHRAESADALAPLLAHCLASPGVHLVDVPIDYSDNEKILNREIPRLSAQL

Sequences:

>Translated_548_residues
MKASDLFVKALEAEGVEYVFGIPGEENLDLLESIRHSRIKLVLTRHEQAAGFMAATYGRLTGKAGVCLSTLGPGATNLVT
AAAYAQLGGMPMLMVTGQKPIKTSKQGHFQIVDVVDMMQPLTKLTRQIVSIGHIPSVVREAFRRAEDERPGATHLELPED
IAHEEGDGKPIPASYSRRPVAEQKAVARAVAAIRQARHPLLMIGAGGNRKTCCKMLREFVDATGIPFFTTQMGKGVIDES
HPLWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRGGDRTVIHVNFLGAQVDPVYFPQIEVVGDIANAVWQIK
EVLAEPPQWDFSRFMLIKARFDAHLRKGEDDPRYPLHPVRVVHDLYGALPADGIACLDNGMYKIWFARYWRAHEPNSLLL
DNALASMGAGLPSAIATKIVHPHRKVVAICGDGGFMMNSQELETAVRLKLDLLVLVLVLRDDAFGMIRWKQENMNFPDYA
MTLDNPDFVAYAQSYGAQGHRAESADALAPLLAHCLASPGVHLVDVPIDYSDNEKILNREIPRLSAQL
>Mature_548_residues
MKASDLFVKALEAEGVEYVFGIPGEENLDLLESIRHSRIKLVLTRHEQAAGFMAATYGRLTGKAGVCLSTLGPGATNLVT
AAAYAQLGGMPMLMVTGQKPIKTSKQGHFQIVDVVDMMQPLTKLTRQIVSIGHIPSVVREAFRRAEDERPGATHLELPED
IAHEEGDGKPIPASYSRRPVAEQKAVARAVAAIRQARHPLLMIGAGGNRKTCCKMLREFVDATGIPFFTTQMGKGVIDES
HPLWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRGGDRTVIHVNFLGAQVDPVYFPQIEVVGDIANAVWQIK
EVLAEPPQWDFSRFMLIKARFDAHLRKGEDDPRYPLHPVRVVHDLYGALPADGIACLDNGMYKIWFARYWRAHEPNSLLL
DNALASMGAGLPSAIATKIVHPHRKVVAICGDGGFMMNSQELETAVRLKLDLLVLVLVLRDDAFGMIRWKQENMNFPDYA
MTLDNPDFVAYAQSYGAQGHRAESADALAPLLAHCLASPGVHLVDVPIDYSDNEKILNREIPRLSAQL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=547, Percent_Identity=26.3254113345521, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI21361361, Length=532, Percent_Identity=26.1278195488722, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1790104, Length=541, Percent_Identity=28.2809611829945, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI87081685, Length=567, Percent_Identity=28.7477954144621, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1786717, Length=551, Percent_Identity=26.3157894736842, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1787096, Length=531, Percent_Identity=25.6120527306968, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788716, Length=471, Percent_Identity=25.9023354564756, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17542570, Length=580, Percent_Identity=25.1724137931034, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=478, Percent_Identity=24.2677824267782, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=478, Percent_Identity=24.2677824267782, Blast_Score=128, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=565, Percent_Identity=29.2035398230088, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6320816, Length=484, Percent_Identity=24.5867768595041, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321524, Length=277, Percent_Identity=27.7978339350181, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=269, Percent_Identity=26.3940520446097, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=277, Percent_Identity=26.3537906137184, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=475, Percent_Identity=27.1578947368421, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60347; Mature: 60347

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASDLFVKALEAEGVEYVFGIPGEENLDLLESIRHSRIKLVLTRHEQAAGFMAATYGRL
CCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCEEEEEEEECHHHHHHHHHHHHHH
TGKAGVCLSTLGPGATNLVTAAAYAQLGGMPMLMVTGQKPIKTSKQGHFQIVDVVDMMQP
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHH
LTKLTRQIVSIGHIPSVVREAFRRAEDERPGATHLELPEDIAHEEGDGKPIPASYSRRPV
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
AEQKAVARAVAAIRQARHPLLMIGAGGNRKTCCKMLREFVDATGIPFFTTQMGKGVIDES
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCC
HPLWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRGGDRTVIHVNFLGAQVDP
CCEEECCCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCEEECCCEEEEEEEEECCCCCC
VYFPQIEVVGDIANAVWQIKEVLAEPPQWDFSRFMLIKARFDAHLRKGEDDPRYPLHPVR
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEHHHHHHHCCCCCCCCCCHHHH
VVHDLYGALPADGIACLDNGMYKIWFARYWRAHEPNSLLLDNALASMGAGLPSAIATKIV
HHHHHHHCCCCCCHHHHCCCCCEEHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHC
HPHRKVVAICGDGGFMMNSQELETAVRLKLDLLVLVLVLRDDAFGMIRWKQENMNFPDYA
CCCCEEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEE
MTLDNPDFVAYAQSYGAQGHRAESADALAPLLAHCLASPGVHLVDVPIDYSDNEKILNRE
EEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHC
IPRLSAQL
CCHHHCCC
>Mature Secondary Structure
MKASDLFVKALEAEGVEYVFGIPGEENLDLLESIRHSRIKLVLTRHEQAAGFMAATYGRL
CCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCEEEEEEEECHHHHHHHHHHHHHH
TGKAGVCLSTLGPGATNLVTAAAYAQLGGMPMLMVTGQKPIKTSKQGHFQIVDVVDMMQP
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHH
LTKLTRQIVSIGHIPSVVREAFRRAEDERPGATHLELPEDIAHEEGDGKPIPASYSRRPV
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
AEQKAVARAVAAIRQARHPLLMIGAGGNRKTCCKMLREFVDATGIPFFTTQMGKGVIDES
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCC
HPLWLGNATLSDGDFVHRAIDHADCIINVGHDVIEKPPFFMRGGDRTVIHVNFLGAQVDP
CCEEECCCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCEEECCCEEEEEEEEECCCCCC
VYFPQIEVVGDIANAVWQIKEVLAEPPQWDFSRFMLIKARFDAHLRKGEDDPRYPLHPVR
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEHHHHHHHCCCCCCCCCCHHHH
VVHDLYGALPADGIACLDNGMYKIWFARYWRAHEPNSLLLDNALASMGAGLPSAIATKIV
HHHHHHHCCCCCCHHHHCCCCCEEHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHC
HPHRKVVAICGDGGFMMNSQELETAVRLKLDLLVLVLVLRDDAFGMIRWKQENMNFPDYA
CCCCEEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEE
MTLDNPDFVAYAQSYGAQGHRAESADALAPLLAHCLASPGVHLVDVPIDYSDNEKILNRE
EEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHC
IPRLSAQL
CCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644303 [H]