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The map label for this gene is arsB [H]

Identifier: 238025029

GI number: 238025029

Start: 2112684

End: 2113937

Strand: Reverse

Name: arsB [H]

Synonym: bglu_2g16860

Alternate gene names: 238025029

Gene position: 2113937-2112684 (Counterclockwise)

Preceding gene: 238025030

Following gene: 238025028

Centisome position: 74.77

GC content: 66.51

Gene sequence:

>1254_bases
ATGAATCCAACTTTCCTCGCATGGGGCATTGCGCTCGCCGCGACGGCCGGCGTCATCCTGCGGCCGTTCCGCTGGCCCGA
AGCGATCTGGGCCGTGCTCGGCGCGCTGATGCTGGTCGTGCTCGGGCTGCTGCCCGCGAAGCTCGCGTGGCAGGCGGTGG
GCAAGGGCACGGACGTCTATCTTTTCCTGATCGGCATGATGCTGCTCTCGGAGCTTGGACGCAGCGAAGGCCTGTTCGAC
TGGGTCGCGGTCCATGCCGTCAATCTGGCCCGGGGATCGCCGCGCAGGCTCTTCCTGCTGGTGTATCTGGTGGGTGTCGT
GGTGACGACGTTCCTGTCCAACGACGCGGCCGCCGTGGTTCTCACGCCGGCGGTGTTCGCCGCCGCGAGAAAGGCGCAAA
CGAAGGCGCTGCCCTTGGTCTATGTCTGCGCATTCATCGCCAACGCGGCGAGTTTCGTCCTGCCGATCTCGAACCCCGCC
AATCTCGTGCTCTATGCCAACCACACGCCGGCGCTCGGTTCGTGGGTGGCACGCTTCGCCTTGCCTTCGGTGCTTGCGAT
TGTCGCCACGTTCCTGATGTTGCGCTGGACGCAGCGGGCCAGTCTCGGTGGTGCGTGCGCGCGCGACTTGCCGGTGGAAG
CGCTCGGCGCGAACGGCAAGCTCACGCTGGCGAGCATCGGCGTGACGGCGGTGGCGCTGCTCGTAGTCTCGGCCTTCGAT
CTGCCGCTCGGTCTGCCCACCGCGGTTCTCGGCATGCTCACCGCGCTGGTCGTGCTGATCAAGAAGCGGCAGTCGCCGCT
CGAGCTCGTCGGCGAGATATCCTGGAGCGTGCTGCCGCTCGTCGCCGGTCTCTTCGTGCTGGTCGAGATGCTTGACCATA
CGGGCGTGATTCGCGAACTCTCCGGCTGGCTGGAGCAGGCTACGCGTCAGAATGCGTCGATCACGGCGGCCTCGGCCGGG
CTGGTCATTGCCTTCGGAAGCAACGCGATCAACAACCTGCCTGCCGGACTGATCGCGAGCGCGACGGCGTTGCAGGCGCA
CGCTCCCGAGCGCGTCATCGACGCGCTGCTGATCGGCGTGGACCTCGGCCCGAATCTGTCGATCACCGGCTCGCTCGCCA
CCATTCTCTGGCTGTCCGCGATTCGACGGGAAGGCGAAGAAGTGAGCTTCGGGCAGTTTCTCAAGGTCGGTGGGCTCGTC
ATGCTGCCCGCGCTCGCGCTGGCGCTCGCCAGCCGCCTGCTGATCGGACATTGA

Upstream 100 bases:

>100_bases
TGTCGATGGGATCACTCGCGCTCTGGCTGCCGTTTAGCGCATCCCTCAAAAAAGCTTGCGGCATTCGCCGCGACGTCATT
GAAGATGGCCCTCTGACCAC

Downstream 100 bases:

>100_bases
TGCTACCAACGCACGGAGGTCGGACCAATGAGCCAGGCATGGATCTATCCCTTCATCATTCTGGGCGGCATCCTGCAGGC
CGCAGGCGCGCCCGTCAACG

Product: Arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNPTFLAWGIALAATAGVILRPFRWPEAIWAVLGALMLVVLGLLPAKLAWQAVGKGTDVYLFLIGMMLLSELGRSEGLFD
WVAVHAVNLARGSPRRLFLLVYLVGVVVTTFLSNDAAAVVLTPAVFAAARKAQTKALPLVYVCAFIANAASFVLPISNPA
NLVLYANHTPALGSWVARFALPSVLAIVATFLMLRWTQRASLGGACARDLPVEALGANGKLTLASIGVTAVALLVVSAFD
LPLGLPTAVLGMLTALVVLIKKRQSPLELVGEISWSVLPLVAGLFVLVEMLDHTGVIRELSGWLEQATRQNASITAASAG
LVIAFGSNAINNLPAGLIASATALQAHAPERVIDALLIGVDLGPNLSITGSLATILWLSAIRREGEEVSFGQFLKVGGLV
MLPALALALASRLLIGH

Sequences:

>Translated_417_residues
MNPTFLAWGIALAATAGVILRPFRWPEAIWAVLGALMLVVLGLLPAKLAWQAVGKGTDVYLFLIGMMLLSELGRSEGLFD
WVAVHAVNLARGSPRRLFLLVYLVGVVVTTFLSNDAAAVVLTPAVFAAARKAQTKALPLVYVCAFIANAASFVLPISNPA
NLVLYANHTPALGSWVARFALPSVLAIVATFLMLRWTQRASLGGACARDLPVEALGANGKLTLASIGVTAVALLVVSAFD
LPLGLPTAVLGMLTALVVLIKKRQSPLELVGEISWSVLPLVAGLFVLVEMLDHTGVIRELSGWLEQATRQNASITAASAG
LVIAFGSNAINNLPAGLIASATALQAHAPERVIDALLIGVDLGPNLSITGSLATILWLSAIRREGEEVSFGQFLKVGGLV
MLPALALALASRLLIGH
>Mature_417_residues
MNPTFLAWGIALAATAGVILRPFRWPEAIWAVLGALMLVVLGLLPAKLAWQAVGKGTDVYLFLIGMMLLSELGRSEGLFD
WVAVHAVNLARGSPRRLFLLVYLVGVVVTTFLSNDAAAVVLTPAVFAAARKAQTKALPLVYVCAFIANAASFVLPISNPA
NLVLYANHTPALGSWVARFALPSVLAIVATFLMLRWTQRASLGGACARDLPVEALGANGKLTLASIGVTAVALLVVSAFD
LPLGLPTAVLGMLTALVVLIKKRQSPLELVGEISWSVLPLVAGLFVLVEMLDHTGVIRELSGWLEQATRQNASITAASAG
LVIAFGSNAINNLPAGLIASATALQAHAPERVIDALLIGVDLGPNLSITGSLATILWLSAIRREGEEVSFGQFLKVGGLV
MLPALALALASRLLIGH

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=398, Percent_Identity=28.391959798995, Blast_Score=121, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 43685; Mature: 43685

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTFLAWGIALAATAGVILRPFRWPEAIWAVLGALMLVVLGLLPAKLAWQAVGKGTDVY
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LFLIGMMLLSELGRSEGLFDWVAVHAVNLARGSPRRLFLLVYLVGVVVTTFLSNDAAAVV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
LTPAVFAAARKAQTKALPLVYVCAFIANAASFVLPISNPANLVLYANHTPALGSWVARFA
EHHHHHHHHHHHHHHCHHHHHHHHHHHCHHHEEEECCCCCCEEEEECCCCHHHHHHHHHH
LPSVLAIVATFLMLRWTQRASLGGACARDLPVEALGANGKLTLASIGVTAVALLVVSAFD
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHC
LPLGLPTAVLGMLTALVVLIKKRQSPLELVGEISWSVLPLVAGLFVLVEMLDHTGVIREL
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SGWLEQATRQNASITAASAGLVIAFGSNAINNLPAGLIASATALQAHAPERVIDALLIGV
HHHHHHHHCCCCCEEEECCCEEEEECCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHC
DLGPNLSITGSLATILWLSAIRREGEEVSFGQFLKVGGLVMLPALALALASRLLIGH
CCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPTFLAWGIALAATAGVILRPFRWPEAIWAVLGALMLVVLGLLPAKLAWQAVGKGTDVY
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LFLIGMMLLSELGRSEGLFDWVAVHAVNLARGSPRRLFLLVYLVGVVVTTFLSNDAAAVV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
LTPAVFAAARKAQTKALPLVYVCAFIANAASFVLPISNPANLVLYANHTPALGSWVARFA
EHHHHHHHHHHHHHHCHHHHHHHHHHHCHHHEEEECCCCCCEEEEECCCCHHHHHHHHHH
LPSVLAIVATFLMLRWTQRASLGGACARDLPVEALGANGKLTLASIGVTAVALLVVSAFD
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHCCCCCCEEEEHHHHHHHHHHHHHHHC
LPLGLPTAVLGMLTALVVLIKKRQSPLELVGEISWSVLPLVAGLFVLVEMLDHTGVIREL
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SGWLEQATRQNASITAASAGLVIAFGSNAINNLPAGLIASATALQAHAPERVIDALLIGV
HHHHHHHHCCCCCEEEECCCEEEEECCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHC
DLGPNLSITGSLATILWLSAIRREGEEVSFGQFLKVGGLVMLPALALALASRLLIGH
CCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]