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The map label for this gene is ogt [H]

Identifier: 238024898

GI number: 238024898

Start: 1950829

End: 1951329

Strand: Reverse

Name: ogt [H]

Synonym: bglu_2g15410

Alternate gene names: 238024898

Gene position: 1951329-1950829 (Counterclockwise)

Preceding gene: 238024900

Following gene: 238024897

Centisome position: 69.02

GC content: 69.26

Gene sequence:

>501_bases
ATGTCTCAGGTATGCATGCGGATGCCGTCGCCGGTCGGCGAGTTGACGCTGGTGGCGCGCGGCGACAAGCTGGTCGCGAT
CCTCTGGGACAACGACCTGCCCACGCGCGTGAAGGTCGGCGCGATGCGCGACGACCCCACGCATCCGACGCTCGTCGAGA
CGGCGCGCCAGCTGCGCGAATACTTCGCCGGCGAGCGCACCGTGTTCGAGATCGCCCTCGAATTCCACGGCACCGAATTC
CAGAAGCGGGTCTGGCACGCGCTGCTGACGATTCCGTTCGGCGAGACGCGCAGCTACCGCGATATCGCCGTGCAGATCGG
CAACCATCGCGCCACGCGCGCGGTGGGCGCCGCCAACGGCCGCAATCCGCTGTCGATCGTCGCGCCGTGCCATCGCGTGA
TCGGCAGCTCGGGCGACTTGACCGGCTTCGCCGGCGGCAAGGATCGCAAGGCCGCGCTGCTCGCGCTCGAAGCGCGTTCG
CGGCAGCCGGCGCTGTTCTGA

Upstream 100 bases:

>100_bases
CGCACGGCGGGGCGCGCGCGTTTGCCGTTACACTGCTGCCTGTCAGTGAGCAACAGCAAGTGCGCAACAGCAAGTGCGCA
ACAGCAGCGAGGAGCAAGCG

Downstream 100 bases:

>100_bases
ACGGCGCCGGCTGCCGCGCTTTCCGGTTGCCGGCTCGCCGCGACGGGCTGCCGGCTGCCTGCCTCCCCGCCACGATACCA
ACCAGAGAGAATCTTGATGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSQVCMRMPSPVGELTLVARGDKLVAILWDNDLPTRVKVGAMRDDPTHPTLVETARQLREYFAGERTVFEIALEFHGTEF
QKRVWHALLTIPFGETRSYRDIAVQIGNHRATRAVGAANGRNPLSIVAPCHRVIGSSGDLTGFAGGKDRKAALLALEARS
RQPALF

Sequences:

>Translated_166_residues
MSQVCMRMPSPVGELTLVARGDKLVAILWDNDLPTRVKVGAMRDDPTHPTLVETARQLREYFAGERTVFEIALEFHGTEF
QKRVWHALLTIPFGETRSYRDIAVQIGNHRATRAVGAANGRNPLSIVAPCHRVIGSSGDLTGFAGGKDRKAALLALEARS
RQPALF
>Mature_165_residues
SQVCMRMPSPVGELTLVARGDKLVAILWDNDLPTRVKVGAMRDDPTHPTLVETARQLREYFAGERTVFEIALEFHGTEFQ
KRVWHALLTIPFGETRSYRDIAVQIGNHRATRAVGAANGRNPLSIVAPCHRVIGSSGDLTGFAGGKDRKAALLALEARSR
QPALF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=114, Percent_Identity=36.8421052631579, Blast_Score=71, Evalue=5e-13,
Organism=Escherichia coli, GI1787596, Length=163, Percent_Identity=41.1042944785276, Blast_Score=107, Evalue=3e-25,
Organism=Escherichia coli, GI1788542, Length=152, Percent_Identity=36.8421052631579, Blast_Score=91, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=52.5, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=52.5, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=91, Percent_Identity=42.8571428571429, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18276; Mature: 18145

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVCMRMPSPVGELTLVARGDKLVAILWDNDLPTRVKVGAMRDDPTHPTLVETARQLRE
CCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
YFAGERTVFEIALEFHGTEFQKRVWHALLTIPFGETRSYRDIAVQIGNHRATRAVGAANG
HHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHEECCCCC
RNPLSIVAPCHRVIGSSGDLTGFAGGKDRKAALLALEARSRQPALF
CCCEEHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SQVCMRMPSPVGELTLVARGDKLVAILWDNDLPTRVKVGAMRDDPTHPTLVETARQLRE
CCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
YFAGERTVFEIALEFHGTEFQKRVWHALLTIPFGETRSYRDIAVQIGNHRATRAVGAANG
HHCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHEECCCCC
RNPLSIVAPCHRVIGSSGDLTGFAGGKDRKAALLALEARSRQPALF
CCCEEHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]