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The map label for this gene is ynfL [H]

Identifier: 238024813

GI number: 238024813

Start: 1850587

End: 1851471

Strand: Direct

Name: ynfL [H]

Synonym: bglu_2g14510

Alternate gene names: 238024813

Gene position: 1850587-1851471 (Clockwise)

Preceding gene: 238024811

Following gene: 238024815

Centisome position: 65.45

GC content: 73.45

Gene sequence:

>885_bases
ATGCGCGAAATCAGTCTTGATCGCCTGCGCACGCTAATCGCGATCGCGGACCTCGGATCGTTCGCGGCGGCCGCACAATC
GCTGCACCTGGCGCCGCCCACCATCAGCCTGCACGTTTCGGAGCTCGAGGCCAGGATCGGCGCGCCGCTGCTGTCCCGCA
CGCGCGGCAACGTGCGGCCGTCGGCGATCGGCGAGGTGCTGATCGAGCGCGCGCGGCGGCTGCTCGCCGATGTCGAGGCG
ACGCTCGACGACGTGGAGCGGCAAGTGCAGGGACTGGCCGGACGGGTGCGGCTAGGCGCATCCACCGGCGCGTTCGCGCA
CCTGCTGCCGCAGGTGCTCGCGCGACTGCGGCGCGACCATCCCGACATCGACGTGCAGATCGCCGTGCTGACCTCGCGCG
ACACGCTCGAACGGCTCACGCACGGCGCGCTGGACATCGGGCTGGTGGCGCTACCGCAGGCCGCCGTGGCGGGGCTGACG
ATCGCGCCGTGGCGTCGCGACCCGGTGGTGGCATTCCTGCCCGCCGACTGGGGCCTGCCGCCCAGTGTGACGCCGGCCTG
GCTCGCCGCGCAGCCGCTGATCCTCAACGACGCCACCACGCGCCTGTCGCGGCAGACTGCCGAATGGTTCGCCGCCGGCG
GCCATCAGCCGGCGCCGCGCATCGAGCTGAACTACAACGACGCGATCAAGAGCCTGGTGGCAGCCGGCTACGGGGCCACG
CTGCTGCCGCACGAGGCCGGCTCGCCGCCACCGGACGCGCGCATCGCGATGCGCCCGCTGCGCCCCGCGCTGTGGCGCGA
ACTCGGCATCGCCCATCGCGCCGGCGCGGCGGAGCTGGCGACACGGCACGTGCTCGACGCGCTGTGGGCGCTGCGGGTGG
ACTGA

Upstream 100 bases:

>100_bases
CCGATCGACGGGAAGCGCGATCATCGCCTGCACGCTAGACTTGTAAAAGTTGAATTTCATCAACATGAGCTTCATTTTTT
CTGAACGAAAAAATTTTTCG

Downstream 100 bases:

>100_bases
ACGCGGCGAGGCCGGACCGGCGTTCCGCCGTTCGCGACACGGGCGCGTCCTGTCTCCACGTCCACGGGTTACGCGACATC
GAGCGCTTCATCGCGACGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MREISLDRLRTLIAIADLGSFAAAAQSLHLAPPTISLHVSELEARIGAPLLSRTRGNVRPSAIGEVLIERARRLLADVEA
TLDDVERQVQGLAGRVRLGASTGAFAHLLPQVLARLRRDHPDIDVQIAVLTSRDTLERLTHGALDIGLVALPQAAVAGLT
IAPWRRDPVVAFLPADWGLPPSVTPAWLAAQPLILNDATTRLSRQTAEWFAAGGHQPAPRIELNYNDAIKSLVAAGYGAT
LLPHEAGSPPPDARIAMRPLRPALWRELGIAHRAGAAELATRHVLDALWALRVD

Sequences:

>Translated_294_residues
MREISLDRLRTLIAIADLGSFAAAAQSLHLAPPTISLHVSELEARIGAPLLSRTRGNVRPSAIGEVLIERARRLLADVEA
TLDDVERQVQGLAGRVRLGASTGAFAHLLPQVLARLRRDHPDIDVQIAVLTSRDTLERLTHGALDIGLVALPQAAVAGLT
IAPWRRDPVVAFLPADWGLPPSVTPAWLAAQPLILNDATTRLSRQTAEWFAAGGHQPAPRIELNYNDAIKSLVAAGYGAT
LLPHEAGSPPPDARIAMRPLRPALWRELGIAHRAGAAELATRHVLDALWALRVD
>Mature_294_residues
MREISLDRLRTLIAIADLGSFAAAAQSLHLAPPTISLHVSELEARIGAPLLSRTRGNVRPSAIGEVLIERARRLLADVEA
TLDDVERQVQGLAGRVRLGASTGAFAHLLPQVLARLRRDHPDIDVQIAVLTSRDTLERLTHGALDIGLVALPQAAVAGLT
IAPWRRDPVVAFLPADWGLPPSVTPAWLAAQPLILNDATTRLSRQTAEWFAAGGHQPAPRIELNYNDAIKSLVAAGYGAT
LLPHEAGSPPPDARIAMRPLRPALWRELGIAHRAGAAELATRHVLDALWALRVD

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=31.7269076305221, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790399, Length=279, Percent_Identity=25.8064516129032, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788748, Length=287, Percent_Identity=29.616724738676, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790208, Length=259, Percent_Identity=26.6409266409266, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367136, Length=255, Percent_Identity=26.2745098039216, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=27.6450511945392, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=26.1224489795918, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787601, Length=153, Percent_Identity=27.4509803921569, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=243, Percent_Identity=24.6913580246914, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081904, Length=148, Percent_Identity=30.4054054054054, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI145693105, Length=286, Percent_Identity=23.4265734265734, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=237, Percent_Identity=23.6286919831224, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790262, Length=277, Percent_Identity=25.2707581227437, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31603; Mature: 31603

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50931 HTH_LYSR ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREISLDRLRTLIAIADLGSFAAAAQSLHLAPPTISLHVSELEARIGAPLLSRTRGNVRP
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCHHHHHCCCCCCH
SAIGEVLIERARRLLADVEATLDDVERQVQGLAGRVRLGASTGAFAHLLPQVLARLRRDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCC
PDIDVQIAVLTSRDTLERLTHGALDIGLVALPQAAVAGLTIAPWRRDPVVAFLPADWGLP
CCCEEEEEEEECHHHHHHHHHCCHHHHHHHHCHHHHCCCEECCCCCCCEEEEECCCCCCC
PSVTPAWLAAQPLILNDATTRLSRQTAEWFAAGGHQPAPRIELNYNDAIKSLVAAGYGAT
CCCCCHHHHCCCEEECHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHCCCCE
LLPHEAGSPPPDARIAMRPLRPALWRELGIAHRAGAAELATRHVLDALWALRVD
ECCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MREISLDRLRTLIAIADLGSFAAAAQSLHLAPPTISLHVSELEARIGAPLLSRTRGNVRP
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCHHHHHCCCCCCH
SAIGEVLIERARRLLADVEATLDDVERQVQGLAGRVRLGASTGAFAHLLPQVLARLRRDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCC
PDIDVQIAVLTSRDTLERLTHGALDIGLVALPQAAVAGLTIAPWRRDPVVAFLPADWGLP
CCCEEEEEEEECHHHHHHHHHCCHHHHHHHHCHHHHCCCEECCCCCCCEEEEECCCCCCC
PSVTPAWLAAQPLILNDATTRLSRQTAEWFAAGGHQPAPRIELNYNDAIKSLVAAGYGAT
CCCCCHHHHCCCEEECHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHCCCCE
LLPHEAGSPPPDARIAMRPLRPALWRELGIAHRAGAAELATRHVLDALWALRVD
ECCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]