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The map label for this gene is nadE [H]

Identifier: 238024670

GI number: 238024670

Start: 1674330

End: 1676444

Strand: Direct

Name: nadE [H]

Synonym: bglu_2g12930

Alternate gene names: 238024670

Gene position: 1674330-1676444 (Clockwise)

Preceding gene: 238024669

Following gene: 238024671

Centisome position: 59.22

GC content: 68.42

Gene sequence:

>2115_bases
ATGAACGATTTCTTCTCGATGTACCGCCACGGCTTCCTGCGGGTGGCCGTCGCCATTCCCCCCGTGGTGGTCGCCGATCC
GGCCGCCAACCTCCAGGCGACGCTGCGCCTCGCGCGCGACGCAGCCGGGCGCGGCGCCCTCGTGACGATCCTGCCCGAAC
TCGGCCTGTCGGCCTACACCAACGACGAGCTGTTCTTCCAGCGCGCGCTGCTCGACGGCGTGAAGCGGGCGATCGGCAAG
CTGCGCGCCGCCTCGGCCGAGCTGCCCGGACTGATCGTGGCGGGCGCGCCGCTCGAATGGAGTGGCCGCCTCTACAACGC
CGCGGTGGTGCTGCACCGCGGCCGGATCCTGGGCGTGGTACCGAAAAGCTACCTGCCGAACTATGGGGAGTTTTATGAGA
AGCGCTACTTCGCTTCCGGGCTCGGCGTGACGGGCGGCACGCTGCGCCTGGACGGCGCCGAGGTGCCGTTCGGCACCGAC
CTGCTGTTTCGCGCCGACGACTATCCGGAGCTCGTGCTCGGCGTGGAGATCTGCGAGGACCTCTGGGCGCCGGTGCCGCC
GTCCACCTATGCGGCACATGCGGGCGCGACCGTGATCGCGAACCTGTCGGCCAGCAACATCACCGTCGGCAAGTCCGAGT
ATCGCCGGCTTCACGTGCGCTCCCACAGCGCGCGCTGCCAGGCCGCCTATCTCTATTCGGCGGCCGGTTGCGGCGAATCC
ACCACCGACCTGGCCTGGGACGGCCACGCACTCGTCTGCGAAAGCGGCGAGATACTGGCCGAAACCGAGCGCTTTGCCGA
CACCGCCCAACTGCTGGTGGCCGATCTCGACCTGCAGCGCATCATGCAGGAGCGCCTGCGCGTGCAGACCTTCGACGATT
GCGCGCGCGCGCTCGGCGGCAGCGCGTTCCGCAACGTCGGCTTCGCGCTCGCTCCGCCGCGCGGCCCGAGCGGGCCGCTG
CAACGCCGCCTCGACCGCTTTCCGTTCGTGCCGGCGGACGTGGCGATGCTCGATGCGAACTGCGAGGAAACCTTCATGAT
CCAGAGTCACGGGCTCGCCAAGCGGCTGCGCGCGACCGGGCTCGAGCAGGTCGTGATCGGCGTGTCGGGCGGGCTCGACT
CCACCTATGCGCTGCTGGTCTGCGCGCTGACCATGGACCGGCTCGGCCTGGACCGCCGCAACATCCTCGCCTACACGCTG
CCCGGCTACGCCACCAGCCGGCACACGCTCGACAATGCCTGGGCGCTGATGCATGTGCTAGGCGTGAGCGCGCGCGAGAT
CGACATCAAGCCGGTATCGGACCGCACGTTGGCAGACATCGGCCATCCGGCCGCACAGGGTGAGGCACGCTATGACGTGA
CCTACGAGAACGTCCAGGCCGGCGCACGCAGCGCCTACCTGTTTCGGCTCGCGAACGCGAATCGCGCCATCGTGATAGGC
ACCGGCGACCTGTCCGAGCTGGCGCTCGGCTGGTGTACCTATGGCGTCGGCGATCAGATGTCGCATTACAACGTGAACGG
CTCGGTGCCGAAAACGCTGATCCAGCAGATGGTTCGCTGGCTGGCGGACCGGCAGCGCTTTGGCGCCGATGCTGCGGGTA
TCCTGCGGCGTATCGTCGAGACCGAGATCTCGCCTGAGCTGGTGCCAGGCAACGCGAGCCAGTCGACCGAGCAGGCGATC
GGCCCCTACGCGTTGCAGGATTTCAACCTCTACCACGTGACGCGCTATGGCTTCGGCCCGGCGAAGATCGCATTCCTCGC
ATGGCATGCGTGGCACGACGCCGAGGCCGGCGACTGGCCACCGCTGATGGACTCGCGTCCTGCCTACGATCTGGCCGCCA
TCAAACGCTGGCTCGTCGTGTTCGTGAAGCGTTTCTTTGAGGGTAGTCAGTTCAAGCGCTCCGCGCTGCCCAACGGCCCG
AAGGTGGCCGCCGGCAGCTCGCTGTCGCCGCGTGGCGACTGGCGCGCGCCGAGCGACTCGGTAGCGCAGGCGTGGCTCGA
CGAACTGACGCGCGCGGTGTCCGATCAGCCTGCCGGCGATGAAGACCGGCGCGAACGTGCTCTGCTTGCTGGCCGACCGC
TCGACGGCGAGCCTGCCGACGGAAACGCGCGATGA

Upstream 100 bases:

>100_bases
GATGCGAATTACGCGCAAGTGCCGCATCGGTTACCTTCGCCGCCAGCGCGGCCGACGCCTCCCGTCATACCCCGCTCGCC
CACCCCGTCCGCCCTCGCCC

Downstream 100 bases:

>100_bases
TTGTAGTCTTCGGCGATCGGCACGCCGATAGCGCCGCTGAAGGCCGCGACGCGATATACCGTCAGCGTGAAGGAACGCTG
CGGCTCGAACCCGTTCGATC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MNDFFSMYRHGFLRVAVAIPPVVVADPAANLQATLRLARDAAGRGALVTILPELGLSAYTNDELFFQRALLDGVKRAIGK
LRAASAELPGLIVAGAPLEWSGRLYNAAVVLHRGRILGVVPKSYLPNYGEFYEKRYFASGLGVTGGTLRLDGAEVPFGTD
LLFRADDYPELVLGVEICEDLWAPVPPSTYAAHAGATVIANLSASNITVGKSEYRRLHVRSHSARCQAAYLYSAAGCGES
TTDLAWDGHALVCESGEILAETERFADTAQLLVADLDLQRIMQERLRVQTFDDCARALGGSAFRNVGFALAPPRGPSGPL
QRRLDRFPFVPADVAMLDANCEETFMIQSHGLAKRLRATGLEQVVIGVSGGLDSTYALLVCALTMDRLGLDRRNILAYTL
PGYATSRHTLDNAWALMHVLGVSAREIDIKPVSDRTLADIGHPAAQGEARYDVTYENVQAGARSAYLFRLANANRAIVIG
TGDLSELALGWCTYGVGDQMSHYNVNGSVPKTLIQQMVRWLADRQRFGADAAGILRRIVETEISPELVPGNASQSTEQAI
GPYALQDFNLYHVTRYGFGPAKIAFLAWHAWHDAEAGDWPPLMDSRPAYDLAAIKRWLVVFVKRFFEGSQFKRSALPNGP
KVAAGSSLSPRGDWRAPSDSVAQAWLDELTRAVSDQPAGDEDRRERALLAGRPLDGEPADGNAR

Sequences:

>Translated_704_residues
MNDFFSMYRHGFLRVAVAIPPVVVADPAANLQATLRLARDAAGRGALVTILPELGLSAYTNDELFFQRALLDGVKRAIGK
LRAASAELPGLIVAGAPLEWSGRLYNAAVVLHRGRILGVVPKSYLPNYGEFYEKRYFASGLGVTGGTLRLDGAEVPFGTD
LLFRADDYPELVLGVEICEDLWAPVPPSTYAAHAGATVIANLSASNITVGKSEYRRLHVRSHSARCQAAYLYSAAGCGES
TTDLAWDGHALVCESGEILAETERFADTAQLLVADLDLQRIMQERLRVQTFDDCARALGGSAFRNVGFALAPPRGPSGPL
QRRLDRFPFVPADVAMLDANCEETFMIQSHGLAKRLRATGLEQVVIGVSGGLDSTYALLVCALTMDRLGLDRRNILAYTL
PGYATSRHTLDNAWALMHVLGVSAREIDIKPVSDRTLADIGHPAAQGEARYDVTYENVQAGARSAYLFRLANANRAIVIG
TGDLSELALGWCTYGVGDQMSHYNVNGSVPKTLIQQMVRWLADRQRFGADAAGILRRIVETEISPELVPGNASQSTEQAI
GPYALQDFNLYHVTRYGFGPAKIAFLAWHAWHDAEAGDWPPLMDSRPAYDLAAIKRWLVVFVKRFFEGSQFKRSALPNGP
KVAAGSSLSPRGDWRAPSDSVAQAWLDELTRAVSDQPAGDEDRRERALLAGRPLDGEPADGNAR
>Mature_704_residues
MNDFFSMYRHGFLRVAVAIPPVVVADPAANLQATLRLARDAAGRGALVTILPELGLSAYTNDELFFQRALLDGVKRAIGK
LRAASAELPGLIVAGAPLEWSGRLYNAAVVLHRGRILGVVPKSYLPNYGEFYEKRYFASGLGVTGGTLRLDGAEVPFGTD
LLFRADDYPELVLGVEICEDLWAPVPPSTYAAHAGATVIANLSASNITVGKSEYRRLHVRSHSARCQAAYLYSAAGCGES
TTDLAWDGHALVCESGEILAETERFADTAQLLVADLDLQRIMQERLRVQTFDDCARALGGSAFRNVGFALAPPRGPSGPL
QRRLDRFPFVPADVAMLDANCEETFMIQSHGLAKRLRATGLEQVVIGVSGGLDSTYALLVCALTMDRLGLDRRNILAYTL
PGYATSRHTLDNAWALMHVLGVSAREIDIKPVSDRTLADIGHPAAQGEARYDVTYENVQAGARSAYLFRLANANRAIVIG
TGDLSELALGWCTYGVGDQMSHYNVNGSVPKTLIQQMVRWLADRQRFGADAAGILRRIVETEISPELVPGNASQSTEQAI
GPYALQDFNLYHVTRYGFGPAKIAFLAWHAWHDAEAGDWPPLMDSRPAYDLAAIKRWLVVFVKRFFEGSQFKRSALPNGP
KVAAGSSLSPRGDWRAPSDSVAQAWLDELTRAVSDQPAGDEDRRERALLAGRPLDGEPADGNAR

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=687, Percent_Identity=23.4352256186317, Blast_Score=97, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17538756, Length=614, Percent_Identity=24.2671009771987, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321865, Length=277, Percent_Identity=26.7148014440433, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24641841, Length=293, Percent_Identity=24.9146757679181, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24641843, Length=293, Percent_Identity=24.9146757679181, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 76495; Mature: 76495

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDFFSMYRHGFLRVAVAIPPVVVADPAANLQATLRLARDAAGRGALVTILPELGLSAYT
CCHHHHHHHHHHEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEHHCCCCCCC
NDELFFQRALLDGVKRAIGKLRAASAELPGLIVAGAPLEWSGRLYNAAVVLHRGRILGVV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCEEEEC
PKSYLPNYGEFYEKRYFASGLGVTGGTLRLDGAEVPFGTDLLFRADDYPELVLGVEICED
CHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHH
LWAPVPPSTYAAHAGATVIANLSASNITVGKSEYRRLHVRSHSARCQAAYLYSAAGCGES
HCCCCCCCCHHHHCCCEEEEECCCCCEEECHHHHHEEEECCCCCCCEEEEEEECCCCCCC
TTDLAWDGHALVCESGEILAETERFADTAQLLVADLDLQRIMQERLRVQTFDDCARALGG
CCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
SAFRNVGFALAPPRGPSGPLQRRLDRFPFVPADVAMLDANCEETFMIQSHGLAKRLRATG
HHHHHCCEEECCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHCC
LEQVVIGVSGGLDSTYALLVCALTMDRLGLDRRNILAYTLPGYATSRHTLDNAWALMHVL
HHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
GVSAREIDIKPVSDRTLADIGHPAAQGEARYDVTYENVQAGARSAYLFRLANANRAIVIG
CCCCCEEEEECCCCCHHHHCCCCCCCCCCEEEEEHHHHHCCCCCEEEEEEECCCEEEEEE
TGDLSELALGWCTYGVGDQMSHYNVNGSVPKTLIQQMVRWLADRQRFGADAAGILRRIVE
CCCHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TEISPELVPGNASQSTEQAIGPYALQDFNLYHVTRYGFGPAKIAFLAWHAWHDAEAGDWP
HCCCCCCCCCCCCCCHHHHCCCEEEECCCEEEEEECCCCHHHEEEEEEECCCCCCCCCCC
PLMDSRPAYDLAAIKRWLVVFVKRFFEGSQFKRSALPNGPKVAAGSSLSPRGDWRAPSDS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
VAQAWLDELTRAVSDQPAGDEDRRERALLAGRPLDGEPADGNAR
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNDFFSMYRHGFLRVAVAIPPVVVADPAANLQATLRLARDAAGRGALVTILPELGLSAYT
CCHHHHHHHHHHEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEHHCCCCCCC
NDELFFQRALLDGVKRAIGKLRAASAELPGLIVAGAPLEWSGRLYNAAVVLHRGRILGVV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCEEEEC
PKSYLPNYGEFYEKRYFASGLGVTGGTLRLDGAEVPFGTDLLFRADDYPELVLGVEICED
CHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHH
LWAPVPPSTYAAHAGATVIANLSASNITVGKSEYRRLHVRSHSARCQAAYLYSAAGCGES
HCCCCCCCCHHHHCCCEEEEECCCCCEEECHHHHHEEEECCCCCCCEEEEEEECCCCCCC
TTDLAWDGHALVCESGEILAETERFADTAQLLVADLDLQRIMQERLRVQTFDDCARALGG
CCCEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
SAFRNVGFALAPPRGPSGPLQRRLDRFPFVPADVAMLDANCEETFMIQSHGLAKRLRATG
HHHHHCCEEECCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHCC
LEQVVIGVSGGLDSTYALLVCALTMDRLGLDRRNILAYTLPGYATSRHTLDNAWALMHVL
HHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
GVSAREIDIKPVSDRTLADIGHPAAQGEARYDVTYENVQAGARSAYLFRLANANRAIVIG
CCCCCEEEEECCCCCHHHHCCCCCCCCCCEEEEEHHHHHCCCCCEEEEEEECCCEEEEEE
TGDLSELALGWCTYGVGDQMSHYNVNGSVPKTLIQQMVRWLADRQRFGADAAGILRRIVE
CCCHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TEISPELVPGNASQSTEQAIGPYALQDFNLYHVTRYGFGPAKIAFLAWHAWHDAEAGDWP
HCCCCCCCCCCCCCCHHHHCCCEEEECCCEEEEEECCCCHHHEEEEEEECCCCCCCCCCC
PLMDSRPAYDLAAIKRWLVVFVKRFFEGSQFKRSALPNGPKVAAGSSLSPRGDWRAPSDS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
VAQAWLDELTRAVSDQPAGDEDRRERALLAGRPLDGEPADGNAR
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]