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The map label for this gene is poxB [H]

Identifier: 238024631

GI number: 238024631

Start: 1596534

End: 1598255

Strand: Reverse

Name: poxB [H]

Synonym: bglu_2g12460

Alternate gene names: 238024631

Gene position: 1598255-1596534 (Counterclockwise)

Preceding gene: 238024632

Following gene: 238024629

Centisome position: 56.53

GC content: 69.22

Gene sequence:

>1722_bases
ATGGCAAGCAAAACGATCGCAGATTATCTGGCCCACACCCTGGCGGAAGCCGGAGTCGAACGCATCTGGGGGGTGACGGG
CGACAGCCTGAACGGCCTGTCGGACAGCCTGCGGCGGCTCGGCAAGATCGAATGGGCGCATACGCGTCACGAGGAGACCG
CGGCGTTCGCGGCCGGCGCGGAAGCGGCCGTCACGGGGCGCCTGGCCGTGTGCGCGGGCAGCTGCGGCCCCGGCAACCTG
CACCTGATCAACGGCCTGTTCGATTGCCATCGCAACCACGTGCCGGTGGTGGCGATCGCCGCGCACATCCCGTCCTCGGA
GATCGGGCTCGGCTATTTCCAGGAGACGCATCCGCAGGAGCTGTTCCGCGAGTGTAGCCATTACGTCGAGCTGGTGTCGA
ACCCGAAGCAGTTCCCGCGCGTGCTCGACCGCGCGATTCGCGCCGCGGTCGAGGAGCGCGGCGTGGCCGTGATCGTGCTG
CCCGGCGACGTCGCGCTCGAGGCGGTGCCCGATGCGGTGCCCGGCCATGTCAGCTACGAGGCCGGCGCGGTGGTGCCGCC
CGAGCCGCAGCTCGACCGGCTCGCGAAGCTGCTCGACGCGTCGCGCAAGGTCGCGCTGCTGTGCGGCAGCGGCTGCGCGG
GCGCGCATGACGAGATCGTGGCGCTGGCCGACACGCTCGGTGCTCCGATCGTCCACGCGCTGCGCGGCAAGGAGCACGTG
GAATGGGACAACCCGTTCGACGTCGGCATGACCGGGCTGATCGGCTTCAGCTCCGGCTATCACGCGCTGAAATCATGCGA
CACGCTGCTGATGCTCGGCACCGATTTCCCCTACCGCAACTTCTATCCGACCGACGCGAAGGTGATCCAGATCGACCGGC
GCGGCGCGGCGATCGGCAAGCGCGTGGCCGTGGACCTGGGGCTCGTCGGCGACGTGAAGGCGACGCTCGCCGCGCTGCAC
GGGCGCCTCGCGCGCAAGACCGAGCGCCATTTCCTCGATGCCGCGCTGCGCCACTACGCCGAGGCGCGCGAGGGGCTCGA
CAGCCTGGCGCGGCCGTCGCCGGTCGGCAAGCCGATCCATCCGCAATACCTGACGAAGCTGGTCAGCGAGCTGGCTGCCG
ACGACGCGATCTTCAGCGTGGACGTGGGCACGCCCACGCTGTGGGCCGCGCGCTACCTGAAGATGAACGGCAAGCGGCGC
CTGCTCGGCTCGTTCAACCATGGCTCGATGGCCAACGCGTTGCCGCAGGCGCTCGGCGCGCAGGCCGCGCAGCCGGACCG
CCAGGTGGTGGCGCTGTGCGGCGACGGCGGGCTGTCGATGCTGATGGGCGAGCTGCTCACGGCGCGCCAGCAGCAGCTGC
CGCTCACCCTGGTGGTGTTCAACAACAGCTCGCTCGGCTTCGTGTCGATGGAGATGAAGGCGGGCGGCTATCTCGACGAC
GACACGCGGCTGGCCGATACCGATTACGCCGCGATCGCGCGCGGCGCGGGGATCGAGGCGATACGCGTCGAACGGTCGGA
GGAACTGGAGGCCGCGTTGCGAAAGGCGTTCGCGCTGCGCAAGCCGGTGCTGGTCGACGTGGTGACGGCGCGGCAGGAGA
TTGCGCTGCCGCCGAAGATCGAATGGGCGCAGGCCAAGGGTTTCAGCCTCTACATGCTGAAGGCCGTGCTCAATGCGAAG
GGCAACGAGGTGGTGGAACTGGCGACGACGATGTTCCGTTGA

Upstream 100 bases:

>100_bases
CGCGCGACCGCGCGGCTGCGCGCCGTCGCGTACCCCGACGTTCGCGCGGGCGCCTCACGAGGGCGGCGCGCGAACTGCCT
TCACTTCGAACGGAGCGCCC

Downstream 100 bases:

>100_bases
GGCGCGTTCGGCTGGAGAGGGCGGCCGGCGGCGCAGGCCGCCGGCGCGGGACACGGCCGCGCACGCGAGGCGCGGCGGCC
GGTTGCGGCTTACTTCGGGT

Product: Thiamine pyrophosphate enzyme-like TPP binding protein

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MASKTIADYLAHTLAEAGVERIWGVTGDSLNGLSDSLRRLGKIEWAHTRHEETAAFAAGAEAAVTGRLAVCAGSCGPGNL
HLINGLFDCHRNHVPVVAIAAHIPSSEIGLGYFQETHPQELFRECSHYVELVSNPKQFPRVLDRAIRAAVEERGVAVIVL
PGDVALEAVPDAVPGHVSYEAGAVVPPEPQLDRLAKLLDASRKVALLCGSGCAGAHDEIVALADTLGAPIVHALRGKEHV
EWDNPFDVGMTGLIGFSSGYHALKSCDTLLMLGTDFPYRNFYPTDAKVIQIDRRGAAIGKRVAVDLGLVGDVKATLAALH
GRLARKTERHFLDAALRHYAEAREGLDSLARPSPVGKPIHPQYLTKLVSELAADDAIFSVDVGTPTLWAARYLKMNGKRR
LLGSFNHGSMANALPQALGAQAAQPDRQVVALCGDGGLSMLMGELLTARQQQLPLTLVVFNNSSLGFVSMEMKAGGYLDD
DTRLADTDYAAIARGAGIEAIRVERSEELEAALRKAFALRKPVLVDVVTARQEIALPPKIEWAQAKGFSLYMLKAVLNAK
GNEVVELATTMFR

Sequences:

>Translated_573_residues
MASKTIADYLAHTLAEAGVERIWGVTGDSLNGLSDSLRRLGKIEWAHTRHEETAAFAAGAEAAVTGRLAVCAGSCGPGNL
HLINGLFDCHRNHVPVVAIAAHIPSSEIGLGYFQETHPQELFRECSHYVELVSNPKQFPRVLDRAIRAAVEERGVAVIVL
PGDVALEAVPDAVPGHVSYEAGAVVPPEPQLDRLAKLLDASRKVALLCGSGCAGAHDEIVALADTLGAPIVHALRGKEHV
EWDNPFDVGMTGLIGFSSGYHALKSCDTLLMLGTDFPYRNFYPTDAKVIQIDRRGAAIGKRVAVDLGLVGDVKATLAALH
GRLARKTERHFLDAALRHYAEAREGLDSLARPSPVGKPIHPQYLTKLVSELAADDAIFSVDVGTPTLWAARYLKMNGKRR
LLGSFNHGSMANALPQALGAQAAQPDRQVVALCGDGGLSMLMGELLTARQQQLPLTLVVFNNSSLGFVSMEMKAGGYLDD
DTRLADTDYAAIARGAGIEAIRVERSEELEAALRKAFALRKPVLVDVVTARQEIALPPKIEWAQAKGFSLYMLKAVLNAK
GNEVVELATTMFR
>Mature_572_residues
ASKTIADYLAHTLAEAGVERIWGVTGDSLNGLSDSLRRLGKIEWAHTRHEETAAFAAGAEAAVTGRLAVCAGSCGPGNLH
LINGLFDCHRNHVPVVAIAAHIPSSEIGLGYFQETHPQELFRECSHYVELVSNPKQFPRVLDRAIRAAVEERGVAVIVLP
GDVALEAVPDAVPGHVSYEAGAVVPPEPQLDRLAKLLDASRKVALLCGSGCAGAHDEIVALADTLGAPIVHALRGKEHVE
WDNPFDVGMTGLIGFSSGYHALKSCDTLLMLGTDFPYRNFYPTDAKVIQIDRRGAAIGKRVAVDLGLVGDVKATLAALHG
RLARKTERHFLDAALRHYAEAREGLDSLARPSPVGKPIHPQYLTKLVSELAADDAIFSVDVGTPTLWAARYLKMNGKRRL
LGSFNHGSMANALPQALGAQAAQPDRQVVALCGDGGLSMLMGELLTARQQQLPLTLVVFNNSSLGFVSMEMKAGGYLDDD
TRLADTDYAAIARGAGIEAIRVERSEELEAALRKAFALRKPVLVDVVTARQEIALPPKIEWAQAKGFSLYMLKAVLNAKG
NEVVELATTMFR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=25.0883392226148, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI21361361, Length=586, Percent_Identity=24.4027303754266, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1787096, Length=568, Percent_Identity=67.4295774647887, Blast_Score=776, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=28.5974499089253, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI87081685, Length=553, Percent_Identity=27.6672694394213, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1786717, Length=564, Percent_Identity=26.241134751773, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1788716, Length=545, Percent_Identity=22.5688073394495, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17531299, Length=550, Percent_Identity=26.9090909090909, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=26.9090909090909, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=556, Percent_Identity=23.3812949640288, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6320816, Length=546, Percent_Identity=23.2600732600733, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=495, Percent_Identity=22.020202020202, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320123, Length=489, Percent_Identity=21.6768916155419, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI19922626, Length=571, Percent_Identity=24.1681260945709, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 61491; Mature: 61360

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKTIADYLAHTLAEAGVERIWGVTGDSLNGLSDSLRRLGKIEWAHTRHEETAAFAAGA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHHHHHCCC
EAAVTGRLAVCAGSCGPGNLHLINGLFDCHRNHVPVVAIAAHIPSSEIGLGYFQETHPQE
CHHHHCEEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHCCCHHH
LFRECSHYVELVSNPKQFPRVLDRAIRAAVEERGVAVIVLPGDVALEAVPDAVPGHVSYE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCCCCCCCCC
AGAVVPPEPQLDRLAKLLDASRKVALLCGSGCAGAHDEIVALADTLGAPIVHALRGKEHV
CCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCHHHHHHHCCCCCC
EWDNPFDVGMTGLIGFSSGYHALKSCDTLLMLGTDFPYRNFYPTDAKVIQIDRRGAAIGK
CCCCCCCCCCHHHEECCCCHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHCC
RVAVDLGLVGDVKATLAALHGRLARKTERHFLDAALRHYAEAREGLDSLARPSPVGKPIH
HHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PQYLTKLVSELAADDAIFSVDVGTPTLWAARYLKMNGKRRLLGSFNHGSMANALPQALGA
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCC
QAAQPDRQVVALCGDGGLSMLMGELLTARQQQLPLTLVVFNNSSLGFVSMEMKAGGYLDD
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCC
DTRLADTDYAAIARGAGIEAIRVERSEELEAALRKAFALRKPVLVDVVTARQEIALPPKI
CCEECCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
EWAQAKGFSLYMLKAVLNAKGNEVVELATTMFR
CHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
ASKTIADYLAHTLAEAGVERIWGVTGDSLNGLSDSLRRLGKIEWAHTRHEETAAFAAGA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHHHHHCCC
EAAVTGRLAVCAGSCGPGNLHLINGLFDCHRNHVPVVAIAAHIPSSEIGLGYFQETHPQE
CHHHHCEEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHCCCHHH
LFRECSHYVELVSNPKQFPRVLDRAIRAAVEERGVAVIVLPGDVALEAVPDAVPGHVSYE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCCCCCCCCCCC
AGAVVPPEPQLDRLAKLLDASRKVALLCGSGCAGAHDEIVALADTLGAPIVHALRGKEHV
CCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCHHHHHHHCCCCCC
EWDNPFDVGMTGLIGFSSGYHALKSCDTLLMLGTDFPYRNFYPTDAKVIQIDRRGAAIGK
CCCCCCCCCCHHHEECCCCHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHCC
RVAVDLGLVGDVKATLAALHGRLARKTERHFLDAALRHYAEAREGLDSLARPSPVGKPIH
HHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PQYLTKLVSELAADDAIFSVDVGTPTLWAARYLKMNGKRRLLGSFNHGSMANALPQALGA
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCC
QAAQPDRQVVALCGDGGLSMLMGELLTARQQQLPLTLVVFNNSSLGFVSMEMKAGGYLDD
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCC
DTRLADTDYAAIARGAGIEAIRVERSEELEAALRKAFALRKPVLVDVVTARQEIALPPKI
CCEECCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
EWAQAKGFSLYMLKAVLNAKGNEVVELATTMFR
CHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]