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The map label for this gene is emrB [H]

Identifier: 23501937

GI number: 23501937

Start: 1029023

End: 1030564

Strand: Reverse

Name: emrB [H]

Synonym: BR1059

Alternate gene names: 23501937

Gene position: 1030564-1029023 (Counterclockwise)

Preceding gene: 23501938

Following gene: 23501936

Centisome position: 48.89

GC content: 56.61

Gene sequence:

>1542_bases
ATGGCCGAGGCAACGGCAATTGCAGCAAGCGGGTCGAAGCGCCTTGCCGTCACCATCTGCGTGATGATGGCGACGCTGAT
GCAGGCGCTGGATTCCACCATCGCCAACGTCGCGCTGCCCTATATGCAGGGAAGCCTTGCGACGACAAGCAGCCAGGTCA
ATTGGGTGCTGACCTCCTATATCGTCGCTGCCGCCATTCTGACACCGGCCACCGGCTGGCTTGAGGAGCGTTTTGGCCGA
CGCCCACTGTTCATCATCTGCGTTGCCGGTTTCGTTATCGCTTCCATGCTGTGCGGCACGGCAACCAGCATTGAACAGAT
GGTCATTTACCGGATGCTGCAAGGGGCTTTCGGCGCCCCCGTTGTGCCGCTTGCGCAGGCCGTTCTCCTCGACAGTTATT
CTGCGCGGATGCGCGGGCAGGCCATGGCGGTTTTCGGCCTTGGCGTCATGCTGGGCCCGATCCTCGGCCCCACTCTCGGT
GGTTGGCTGACGCAATATTATGATTGGCGCTGGGTCTTTTATGTCAATGTACCGCTTGGCGTGATGACCTTGCTGCTGGC
TTTTGGATTTCTCGAAGGCAAGAAGGGCTCGTCGCTTGCCAGGCTGGACTGGCTGGGGTTTGCAACGCTTGGCCTTGCCA
TTGCGGCATTGCAGCTCTTTCTTGATCGCGGCGAACAGTTGAACTGGTTCGACTCGACTGAAATAAAGACCGAATTCATC
CTTACCATTCTGGGGCTTTATCTCTTCATCGTGCATACCGTGACAGCGCGAAAGCCCTTTCTCGACAAACGCCTGTTTGC
CGACCGCAATTTTGTCATGGGGCAAGTGCTGATCTTTGTCATCGGCGCCGTCTTGCTGGCGACATTGTCGCTGCTGGCGC
CCTATCTCGAACGGTTGATGGGATATTCTGTTCTGATGGCCGGGCTGGTGCTCGCTCCGCGCGGGGTTGGAACAATGGTG
GCGATGGTCCTGGTGGGGCGGCTGACCTCCGTGGTCGATATAAAATATCTTCTTCTGACCGGCCTGGCACTGACCGCCGT
TGCACTTCACCAGATGAGCATGTTTACGCCCGATGTCTCGCAAAGCACGATCATATGGAGCGGAATCATACAGGGGTTCG
GTCTTGGATTCGTATTTGTGCCGCTCAGCACCATTACATTTGTGACGCTGCCGGCGGACCTGCACACACAAGGAACCGGC
TTTTTCAGCCTCATGCGCAATGTGGGTTCAAGCGTGGGCATCTCCGTCACGACATTCCTGCTGTCGCAGAATGCGGCAAT
CGTCCATGCCGGGCTGACGAATGATATTTCACCCTATAGCCGCGCTACCCAGCATTTCAGTTCGGCCGTTCATCTCGACA
CACTGGCTGGCAAGGCCATGCTCAACGAGTTGATTACCGTTCAGGCCGAAACCATTGCCTATGCCGATAATTTTCGCCTG
ATGATGTATATTACTATCTTGACTATGCCGCTCGTTTTCTTCCTGCGGCACACCAAACCGCAGAAAACCAGCTCGGCCGG
CAAGGCGGTAACGGCGCAATAA

Upstream 100 bases:

>100_bases
TGCGTGATGGCGGCAGCGCAACCGTTACCATCAATACAGGCTATCATCGCACATTTGCTACCTTGTGGCGCGATCTGACC
GGCATGGTGGGGATCGACTG

Downstream 100 bases:

>100_bases
TCCCTATTAATGCCGGTGGTAAACTAAAGCCATTGATCCTAATCATTCCAGGTCGCAATTGCTTCATGCGAAGGGATAAT
TTCGCATGAAGCAATTGCAG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MAEATAIAASGSKRLAVTICVMMATLMQALDSTIANVALPYMQGSLATTSSQVNWVLTSYIVAAAILTPATGWLEERFGR
RPLFIICVAGFVIASMLCGTATSIEQMVIYRMLQGAFGAPVVPLAQAVLLDSYSARMRGQAMAVFGLGVMLGPILGPTLG
GWLTQYYDWRWVFYVNVPLGVMTLLLAFGFLEGKKGSSLARLDWLGFATLGLAIAALQLFLDRGEQLNWFDSTEIKTEFI
LTILGLYLFIVHTVTARKPFLDKRLFADRNFVMGQVLIFVIGAVLLATLSLLAPYLERLMGYSVLMAGLVLAPRGVGTMV
AMVLVGRLTSVVDIKYLLLTGLALTAVALHQMSMFTPDVSQSTIIWSGIIQGFGLGFVFVPLSTITFVTLPADLHTQGTG
FFSLMRNVGSSVGISVTTFLLSQNAAIVHAGLTNDISPYSRATQHFSSAVHLDTLAGKAMLNELITVQAETIAYADNFRL
MMYITILTMPLVFFLRHTKPQKTSSAGKAVTAQ

Sequences:

>Translated_513_residues
MAEATAIAASGSKRLAVTICVMMATLMQALDSTIANVALPYMQGSLATTSSQVNWVLTSYIVAAAILTPATGWLEERFGR
RPLFIICVAGFVIASMLCGTATSIEQMVIYRMLQGAFGAPVVPLAQAVLLDSYSARMRGQAMAVFGLGVMLGPILGPTLG
GWLTQYYDWRWVFYVNVPLGVMTLLLAFGFLEGKKGSSLARLDWLGFATLGLAIAALQLFLDRGEQLNWFDSTEIKTEFI
LTILGLYLFIVHTVTARKPFLDKRLFADRNFVMGQVLIFVIGAVLLATLSLLAPYLERLMGYSVLMAGLVLAPRGVGTMV
AMVLVGRLTSVVDIKYLLLTGLALTAVALHQMSMFTPDVSQSTIIWSGIIQGFGLGFVFVPLSTITFVTLPADLHTQGTG
FFSLMRNVGSSVGISVTTFLLSQNAAIVHAGLTNDISPYSRATQHFSSAVHLDTLAGKAMLNELITVQAETIAYADNFRL
MMYITILTMPLVFFLRHTKPQKTSSAGKAVTAQ
>Mature_512_residues
AEATAIAASGSKRLAVTICVMMATLMQALDSTIANVALPYMQGSLATTSSQVNWVLTSYIVAAAILTPATGWLEERFGRR
PLFIICVAGFVIASMLCGTATSIEQMVIYRMLQGAFGAPVVPLAQAVLLDSYSARMRGQAMAVFGLGVMLGPILGPTLGG
WLTQYYDWRWVFYVNVPLGVMTLLLAFGFLEGKKGSSLARLDWLGFATLGLAIAALQLFLDRGEQLNWFDSTEIKTEFIL
TILGLYLFIVHTVTARKPFLDKRLFADRNFVMGQVLIFVIGAVLLATLSLLAPYLERLMGYSVLMAGLVLAPRGVGTMVA
MVLVGRLTSVVDIKYLLLTGLALTAVALHQMSMFTPDVSQSTIIWSGIIQGFGLGFVFVPLSTITFVTLPADLHTQGTGF
FSLMRNVGSSVGISVTTFLLSQNAAIVHAGLTNDISPYSRATQHFSSAVHLDTLAGKAMLNELITVQAETIAYADNFRLM
MYITILTMPLVFFLRHTKPQKTSSAGKAVTAQ

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=492, Percent_Identity=32.7235772357724, Blast_Score=283, Evalue=3e-77,
Organism=Escherichia coli, GI1788710, Length=503, Percent_Identity=32.4055666003976, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1790195, Length=413, Percent_Identity=30.0242130750605, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI87081983, Length=421, Percent_Identity=28.5035629453682, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788392, Length=430, Percent_Identity=28.1395348837209, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI87082320, Length=201, Percent_Identity=22.8855721393035, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1790146, Length=167, Percent_Identity=25.748502994012, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=461, Percent_Identity=22.5596529284165, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323000, Length=102, Percent_Identity=34.3137254901961, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=144, Percent_Identity=26.3888888888889, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=130, Percent_Identity=27.6923076923077, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55441; Mature: 55309

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEATAIAASGSKRLAVTICVMMATLMQALDSTIANVALPYMQGSLATTSSQVNWVLTSY
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
IVAAAILTPATGWLEERFGRRPLFIICVAGFVIASMLCGTATSIEQMVIYRMLQGAFGAP
HHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
VVPLAQAVLLDSYSARMRGQAMAVFGLGVMLGPILGPTLGGWLTQYYDWRWVFYVNVPLG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHH
VMTLLLAFGFLEGKKGSSLARLDWLGFATLGLAIAALQLFLDRGEQLNWFDSTEIKTEFI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
LTILGLYLFIVHTVTARKPFLDKRLFADRNFVMGQVLIFVIGAVLLATLSLLAPYLERLM
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYSVLMAGLVLAPRGVGTMVAMVLVGRLTSVVDIKYLLLTGLALTAVALHQMSMFTPDVS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QSTIIWSGIIQGFGLGFVFVPLSTITFVTLPADLHTQGTGFFSLMRNVGSSVGISVTTFL
CCHHHHHHHHHHHCCHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHHH
LSQNAAIVHAGLTNDISPYSRATQHFSSAVHLDTLAGKAMLNELITVQAETIAYADNFRL
HHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHH
MMYITILTMPLVFFLRHTKPQKTSSAGKAVTAQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEATAIAASGSKRLAVTICVMMATLMQALDSTIANVALPYMQGSLATTSSQVNWVLTSY
CCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH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HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]