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The map label for this gene is opuAB [H]

Identifier: 23100910

GI number: 23100910

Start: 3592977

End: 3593837

Strand: Reverse

Name: opuAB [H]

Synonym: OB3455

Alternate gene names: 23100910

Gene position: 3593837-3592977 (Counterclockwise)

Preceding gene: 23100911

Following gene: 23100909

Centisome position: 98.99

GC content: 37.28

Gene sequence:

>861_bases
ATGAGTAATGAAAGCATGCTTGATATTGTACCAGAAATACCATTATCACAATGGACAGAAACAACAACAGAATGGATTCA
AGAGAAATTTGCATTTATTTTCGAACCATTGAAGACATATTTTGGCGATTTTATGGATTATGTATCAGAGGAATTACTTG
TAGGAATTCCACCCGTACTATTTATCCTCATTGTTGCCGTAATTGCATTTTTTGTATCGGGTAAGAAATTTGGATTAGCG
GCCTTTTCGATTGTTGGTCTTTGGTTTGTATATAACCAAGGTTTATGGGATGCGCTAATGTCTACCGTTACCCTTGTATT
ATTAGCAAGTTTATTATCCGTTATCGTTGGTGTTCCGATTGGAATACTGATGTCAAAGAGTAAGATAGCGAATACCATAT
TCACACCAATTCTAGACTTTATGCAGACGATGCCAGCATTCGTATACTTAATTCCTGCTGTAGCATTCTTCGGAATTGGT
ATGGTTCCAGGTGTGTTTGCATCACTAATCTTTGCAACACCACCAACCGTTCGTTTTACGAATTTAGGTATTCGTCAAGT
ATCGAAGGATCTTGTAGAAGCATCAGATTCATTTGGTAGTACAGGAACTCAAAAACTATTTAAGGTTGAATTACCAATGG
CGAGATCGACGATTATGGCTGGGATTAACCAGACTGTAATGTTAGCACTATCCATGGTTGTAATTGCATCGATGATTGGT
GCACCTGGATTAGGAAGTCAGGTACTTTCTTCTCTACAACGTGCTTCTGTAGGACCTGGTTTCGTAGCAGGTATTGGAAT
TGTTATCTTAGCGATTATTATTGATCGTATTACGCAAAATATGAACAAAGAAAAAGAATAA

Upstream 100 bases:

>100_bases
GTTCCGTTAGCAGTGGTAGATAAAGATGATCGTTTAGAAGGTATTATCGTTCGTGGAGCTGTGCTTGCCGCTCTTGCTGG
AGATGGAGAGGTGAATACAG

Downstream 100 bases:

>100_bases
CCTTGTGGGTCACCTCTTTTCATGTATTTCATGACGAGAGTCTATATAACTAATCTATACTAAAAGGAGAGATTTTTATG
GCTACATGGAGCTTAAAACG

Product: glycine betaine ABC transporter permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSNESMLDIVPEIPLSQWTETTTEWIQEKFAFIFEPLKTYFGDFMDYVSEELLVGIPPVLFILIVAVIAFFVSGKKFGLA
AFSIVGLWFVYNQGLWDALMSTVTLVLLASLLSVIVGVPIGILMSKSKIANTIFTPILDFMQTMPAFVYLIPAVAFFGIG
MVPGVFASLIFATPPTVRFTNLGIRQVSKDLVEASDSFGSTGTQKLFKVELPMARSTIMAGINQTVMLALSMVVIASMIG
APGLGSQVLSSLQRASVGPGFVAGIGIVILAIIIDRITQNMNKEKE

Sequences:

>Translated_286_residues
MSNESMLDIVPEIPLSQWTETTTEWIQEKFAFIFEPLKTYFGDFMDYVSEELLVGIPPVLFILIVAVIAFFVSGKKFGLA
AFSIVGLWFVYNQGLWDALMSTVTLVLLASLLSVIVGVPIGILMSKSKIANTIFTPILDFMQTMPAFVYLIPAVAFFGIG
MVPGVFASLIFATPPTVRFTNLGIRQVSKDLVEASDSFGSTGTQKLFKVELPMARSTIMAGINQTVMLALSMVVIASMIG
APGLGSQVLSSLQRASVGPGFVAGIGIVILAIIIDRITQNMNKEKE
>Mature_285_residues
SNESMLDIVPEIPLSQWTETTTEWIQEKFAFIFEPLKTYFGDFMDYVSEELLVGIPPVLFILIVAVIAFFVSGKKFGLAA
FSIVGLWFVYNQGLWDALMSTVTLVLLASLLSVIVGVPIGILMSKSKIANTIFTPILDFMQTMPAFVYLIPAVAFFGIGM
VPGVFASLIFATPPTVRFTNLGIRQVSKDLVEASDSFGSTGTQKLFKVELPMARSTIMAGINQTVMLALSMVVIASMIGA
PGLGSQVLSSLQRASVGPGFVAGIGIVILAIIIDRITQNMNKEKE

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=274, Percent_Identity=47.4452554744526, Blast_Score=224, Evalue=3e-60,
Organism=Escherichia coli, GI1788451, Length=195, Percent_Identity=28.7179487179487, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788449, Length=197, Percent_Identity=28.4263959390863, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786564, Length=158, Percent_Identity=30.379746835443, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30994; Mature: 30863

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNESMLDIVPEIPLSQWTETTTEWIQEKFAFIFEPLKTYFGDFMDYVSEELLVGIPPVL
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FILIVAVIAFFVSGKKFGLAAFSIVGLWFVYNQGLWDALMSTVTLVLLASLLSVIVGVPI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GILMSKSKIANTIFTPILDFMQTMPAFVYLIPAVAFFGIGMVPGVFASLIFATPPTVRFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEH
NLGIRQVSKDLVEASDSFGSTGTQKLFKVELPMARSTIMAGINQTVMLALSMVVIASMIG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APGLGSQVLSSLQRASVGPGFVAGIGIVILAIIIDRITQNMNKEKE
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]