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The map label for this gene is citS [H]

Identifier: 23100706

GI number: 23100706

Start: 3388277

End: 3389863

Strand: Reverse

Name: citS [H]

Synonym: OB3251

Alternate gene names: 23100706

Gene position: 3389863-3388277 (Counterclockwise)

Preceding gene: 23100707

Following gene: 23100705

Centisome position: 93.37

GC content: 38.82

Gene sequence:

>1587_bases
ATGAAATGGTCGTTAGAGAGAAAATTTCTTGTGCTTGGTTGTATCCTTGTTACCGCCATCATGTTAATGGTAATGGTGTT
GTATATTTCCTATGAAAGAGACCAGACAAGACAACTGATTGGTCAGCAAGCACTGACTACCGCAATTGCTGTGTCGGAAA
TTCCAGAAGTGCAACATGTCATTGAACACCAAAAGGATGCAGATGTGCTGCAACCATTTATTGAGCGGATTCGAAAACAA
TCAAATGCAGAGTTTATCGTCATAGGTGACCGTAATTCACTTCGGTACACACACCCTGATCCAGACAAAGTCGGTATGCA
AATGGTTGGCGGGGATAATGAACAAGCTTTGGTGGACGGGGAGAATTATGTATCTATAGCAAATGGAAGTTTAGGGGCAT
CTGTACGCGGGAAGTCACCGATTTTCAACAGTGATGGCGATATTATTGGAATCGTATCGGTCGGATATATGATTTCCTAT
GTAGATTCTTTATTTAAACAAGGTCTTATCGGATTTTTAGTATGGTTATCGCTAATATTTATCATTGGTGTCGCAGGAAG
CTTTTTATTAGCAAAAAGTATTCGTAAAGATACGTTTGGTCTAGAGCCGTATCAAATCGCTCGAATATACAAAGAACGTG
GAGCAATCCTGGAATCAATAAAAGAAGGATTAATTGCCACCGATCAACGTGGGCATATAACATTGGTCAATTACTCTGCG
AAAGAAATGTTACATATACGCCATGATGTCATTGGCAAACCGGTACAAGAGGTACTACCGAATACAGAGCTAGCATATGT
CTTAACTGAAGAACATCGAGAGGGTTCTTTTGAAACAACGATTAACCAAACCGTATTACTCGTACGCTATAAATTAATAG
ATGACGATGGGGACTATGGAGGCAAAGTTGCTAGCTTTCAAGTAAGATCGGATTTGCAAGAACTTATTCATACCTTATCT
GAAGTCCAGCAATATTCACAAGATTTACGGGCGCAGACACATGAGTATACCAATAAGCTCTACGTCATTTCTGGTTGGTT
ACAGCTTGGGCATACGGATAAGGCGAAGCAATTTATCCATGAAGAGGTTGGAAAACAGCAGAGTTATGAGAAGGTACTAT
TTGAACAAATCAGTGATTCTACGATACAAGCAATCTTAATTGGAAAATTATCAAAAGCTTCGGAGAAAAAGATAGAATTT
ACTATTAATGAAGGTAGCCATATTAATTATCAATGGCCAGAACAGATGACAGCGCAACTAGTGACAATCATTGGTAATAT
TATAGATAATGCTTTTGATGCCGTTGTGAATACAGACAATCCAAAGATAGATATTTTTCTTACTGATATGGGAAATGACT
TAGTTATTGAGGTAGCTGATAATGGTACAGGCATTAGTCCGGCTGATTACGATAATATTATGAAACAAGGTTATTCCACG
AAAGATGGGCAGAATCGTGGATATGGGTTAGCGCTTGTACAAGCAGCTTTATTAGAGTTACAAGGCTTTTTGGAAATAAC
AGCTAATGAACCGAATGGAACCGTATTTATCGTGTATATTCCGAAAAAAGGGGGAGCGGAATCATGA

Upstream 100 bases:

>100_bases
GAATACATAAAAAGGGAGAAATAGTCTATCTTGATTTATTCGTATCCCCTTACCTTTTATACCCATAGTCGCTATACTAT
TTGTATGGGAGGATAAGGGA

Downstream 100 bases:

>100_bases
ATGTTCTTGTTGCAGAGGATGATTATCGGGTTGCCGATATTCATTGTCAATACTTAGAGAAGTTTCAAGATATACAATAT
ATTGAAAGAGCTGCAACCGC

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKWSLERKFLVLGCILVTAIMLMVMVLYISYERDQTRQLIGQQALTTAIAVSEIPEVQHVIEHQKDADVLQPFIERIRKQ
SNAEFIVIGDRNSLRYTHPDPDKVGMQMVGGDNEQALVDGENYVSIANGSLGASVRGKSPIFNSDGDIIGIVSVGYMISY
VDSLFKQGLIGFLVWLSLIFIIGVAGSFLLAKSIRKDTFGLEPYQIARIYKERGAILESIKEGLIATDQRGHITLVNYSA
KEMLHIRHDVIGKPVQEVLPNTELAYVLTEEHREGSFETTINQTVLLVRYKLIDDDGDYGGKVASFQVRSDLQELIHTLS
EVQQYSQDLRAQTHEYTNKLYVISGWLQLGHTDKAKQFIHEEVGKQQSYEKVLFEQISDSTIQAILIGKLSKASEKKIEF
TINEGSHINYQWPEQMTAQLVTIIGNIIDNAFDAVVNTDNPKIDIFLTDMGNDLVIEVADNGTGISPADYDNIMKQGYST
KDGQNRGYGLALVQAALLELQGFLEITANEPNGTVFIVYIPKKGGAES

Sequences:

>Translated_528_residues
MKWSLERKFLVLGCILVTAIMLMVMVLYISYERDQTRQLIGQQALTTAIAVSEIPEVQHVIEHQKDADVLQPFIERIRKQ
SNAEFIVIGDRNSLRYTHPDPDKVGMQMVGGDNEQALVDGENYVSIANGSLGASVRGKSPIFNSDGDIIGIVSVGYMISY
VDSLFKQGLIGFLVWLSLIFIIGVAGSFLLAKSIRKDTFGLEPYQIARIYKERGAILESIKEGLIATDQRGHITLVNYSA
KEMLHIRHDVIGKPVQEVLPNTELAYVLTEEHREGSFETTINQTVLLVRYKLIDDDGDYGGKVASFQVRSDLQELIHTLS
EVQQYSQDLRAQTHEYTNKLYVISGWLQLGHTDKAKQFIHEEVGKQQSYEKVLFEQISDSTIQAILIGKLSKASEKKIEF
TINEGSHINYQWPEQMTAQLVTIIGNIIDNAFDAVVNTDNPKIDIFLTDMGNDLVIEVADNGTGISPADYDNIMKQGYST
KDGQNRGYGLALVQAALLELQGFLEITANEPNGTVFIVYIPKKGGAES
>Mature_528_residues
MKWSLERKFLVLGCILVTAIMLMVMVLYISYERDQTRQLIGQQALTTAIAVSEIPEVQHVIEHQKDADVLQPFIERIRKQ
SNAEFIVIGDRNSLRYTHPDPDKVGMQMVGGDNEQALVDGENYVSIANGSLGASVRGKSPIFNSDGDIIGIVSVGYMISY
VDSLFKQGLIGFLVWLSLIFIIGVAGSFLLAKSIRKDTFGLEPYQIARIYKERGAILESIKEGLIATDQRGHITLVNYSA
KEMLHIRHDVIGKPVQEVLPNTELAYVLTEEHREGSFETTINQTVLLVRYKLIDDDGDYGGKVASFQVRSDLQELIHTLS
EVQQYSQDLRAQTHEYTNKLYVISGWLQLGHTDKAKQFIHEEVGKQQSYEKVLFEQISDSTIQAILIGKLSKASEKKIEF
TINEGSHINYQWPEQMTAQLVTIIGNIIDNAFDAVVNTDNPKIDIFLTDMGNDLVIEVADNGTGISPADYDNIMKQGYST
KDGQNRGYGLALVQAALLELQGFLEITANEPNGTVFIVYIPKKGGAES

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=524, Percent_Identity=31.4885496183206, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI1790567, Length=512, Percent_Identity=31.0546875, Blast_Score=249, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58901; Mature: 58901

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWSLERKFLVLGCILVTAIMLMVMVLYISYERDQTRQLIGQQALTTAIAVSEIPEVQHV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHH
IEHQKDADVLQPFIERIRKQSNAEFIVIGDRNSLRYTHPDPDKVGMQMVGGDNEQALVDG
HHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHCEEEECCCCCCEEECC
ENYVSIANGSLGASVRGKSPIFNSDGDIIGIVSVGYMISYVDSLFKQGLIGFLVWLSLIF
CCEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IIGVAGSFLLAKSIRKDTFGLEPYQIARIYKERGAILESIKEGLIATDQRGHITLVNYSA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEECCH
KEMLHIRHDVIGKPVQEVLPNTELAYVLTEEHREGSFETTINQTVLLVRYKLIDDDGDYG
HHHHHHHHHHCCCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHEEEEEEEEEECCCCCCC
GKVASFQVRSDLQELIHTLSEVQQYSQDLRAQTHEYTNKLYVISGWLQLGHTDKAKQFIH
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHH
EEVGKQQSYEKVLFEQISDSTIQAILIGKLSKASEKKIEFTINEGSHINYQWPEQMTAQL
HHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCEECEECHHHHHHHH
VTIIGNIIDNAFDAVVNTDNPKIDIFLTDMGNDLVIEVADNGTGISPADYDNIMKQGYST
HHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHCCCCC
KDGQNRGYGLALVQAALLELQGFLEITANEPNGTVFIVYIPKKGGAES
CCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MKWSLERKFLVLGCILVTAIMLMVMVLYISYERDQTRQLIGQQALTTAIAVSEIPEVQHV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHH
IEHQKDADVLQPFIERIRKQSNAEFIVIGDRNSLRYTHPDPDKVGMQMVGGDNEQALVDG
HHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHCEEEECCCCCCEEECC
ENYVSIANGSLGASVRGKSPIFNSDGDIIGIVSVGYMISYVDSLFKQGLIGFLVWLSLIF
CCEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
IIGVAGSFLLAKSIRKDTFGLEPYQIARIYKERGAILESIKEGLIATDQRGHITLVNYSA
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEECCH
KEMLHIRHDVIGKPVQEVLPNTELAYVLTEEHREGSFETTINQTVLLVRYKLIDDDGDYG
HHHHHHHHHHCCCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHEEEEEEEEEECCCCCCC
GKVASFQVRSDLQELIHTLSEVQQYSQDLRAQTHEYTNKLYVISGWLQLGHTDKAKQFIH
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHH
EEVGKQQSYEKVLFEQISDSTIQAILIGKLSKASEKKIEFTINEGSHINYQWPEQMTAQL
HHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCEECEECHHHHHHHH
VTIIGNIIDNAFDAVVNTDNPKIDIFLTDMGNDLVIEVADNGTGISPADYDNIMKQGYST
HHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHCCCCC
KDGQNRGYGLALVQAALLELQGFLEITANEPNGTVFIVYIPKKGGAES
CCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]