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The map label for this gene is thlA [H]

Identifier: 23100087

GI number: 23100087

Start: 2699667

End: 2700851

Strand: Reverse

Name: thlA [H]

Synonym: OB2632

Alternate gene names: 23100087

Gene position: 2700851-2699667 (Counterclockwise)

Preceding gene: 23100088

Following gene: 23100086

Centisome position: 74.39

GC content: 38.31

Gene sequence:

>1185_bases
ATGAAAGAAGTAGTGATTGTTAGTGCTGTCCGGACTGCTACAGGAAGTTTTATGGGGCTTTTAAGTGATATACCTGCGAC
AAAACTTGGTAGTATTGTAATGAAAGAAGCACTTCACCGTGCAAATGTTAAACCAGAAGAAGTAGAAGATGTGATCTTTG
GTAATGTATTACAAGCTGGGCTTGGTCAAGGGACAGCTAGACAAGCAGCAATACAAGCAGGTATTCCTGAATATGTTCCT
GCTACTACGATTAATAAATTATGTGGGTCAGGGTTAAAAAGTGTTCATCTCGCTGCACAAGCTATACAAACTGGAGACGC
CGACATCGTTTTAGCAGGTGGAATGGAGAATATGAGCCAAGCTCCTTATCTTTTAGAGAAAGGGAGACAAGGCTTTCGCA
TGGGTGATGGAAAAGTAGTAGATAGTATGATAAAAGATGGTCTTTGGTGTGCAATTAATGATGTTCATATGGGAATCACT
GCAGAAAATATAGCTGATAAATATCAAATAAGTCGAAAAGAACAGGATAAATTTGCAGCATGGAGCCAACAAAAAACAGA
AAAAGCTATCGAGCAAGGAGCTTTTTCTGATGAAATTGTACCTGTTAAAATTCGAAAATCAAAAAGTGAAGAATTCATCT
TTAATAAAGATGAATTTCCTAGGAGTGGGGTAACAGTAGAAAAACTAGCTAATTTACGCCCTGCTTTTAAAAAGGATGGA
ACAGTAACGGCTGGGAATTCATCTGGAATAAATGATGGTGCAGCTGCACTCGTATTAATGAGTAAGGAAAAGGCGAATGC
GTTAAATATTAAACCAATGGCTACATTACGCGGAAATGCTACTGCGGGAGTAGATTCTAATTTCATGGGAATTGGTCCAG
TTCCAGCAACTAATAAAGCATTGAAAAAAGCGAATATTTCTATTCAAGAAATTGGATTAATCGAAGCTAATGAAGCATTT
GCATCGCAAGCATTAGCTGTTCAAAAAGAACTGAATATTAATCCTGACATTTTAAATATTCATGGTGGAGCAATTTCTCT
AGGTCATCCAATTGGTGCAAGTGGAGCACGTATTCTTGTTACGTTGCTACATGCGATGAAAATGCAGAGCACACAATTTG
GTTTAGCAACGCTTTGTATTGGTGGTGGAATGGGCGCTGCTAGTATCGTAGAATTGGAAAAGTAG

Upstream 100 bases:

>100_bases
CCAGGTGTTGGTGTAGATGAAGTACGTGATAAAACAGAAGCTGATTTTAATATTCACGAGGATATTAAAACAAAGATAAA
TTAGATAGGAGAGTTGGATG

Downstream 100 bases:

>100_bases
TTTATAAATTTTTAAATTAACGGAGGAATCATAATGGTAAAAGATAGAGTTGTTTATATAACTGGTGCGGCAAGTGGCAT
TGGTTACCAAATGGGCGTGG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKEVVIVSAVRTATGSFMGLLSDIPATKLGSIVMKEALHRANVKPEEVEDVIFGNVLQAGLGQGTARQAAIQAGIPEYVP
ATTINKLCGSGLKSVHLAAQAIQTGDADIVLAGGMENMSQAPYLLEKGRQGFRMGDGKVVDSMIKDGLWCAINDVHMGIT
AENIADKYQISRKEQDKFAAWSQQKTEKAIEQGAFSDEIVPVKIRKSKSEEFIFNKDEFPRSGVTVEKLANLRPAFKKDG
TVTAGNSSGINDGAAALVLMSKEKANALNIKPMATLRGNATAGVDSNFMGIGPVPATNKALKKANISIQEIGLIEANEAF
ASQALAVQKELNINPDILNIHGGAISLGHPIGASGARILVTLLHAMKMQSTQFGLATLCIGGGMGAASIVELEK

Sequences:

>Translated_394_residues
MKEVVIVSAVRTATGSFMGLLSDIPATKLGSIVMKEALHRANVKPEEVEDVIFGNVLQAGLGQGTARQAAIQAGIPEYVP
ATTINKLCGSGLKSVHLAAQAIQTGDADIVLAGGMENMSQAPYLLEKGRQGFRMGDGKVVDSMIKDGLWCAINDVHMGIT
AENIADKYQISRKEQDKFAAWSQQKTEKAIEQGAFSDEIVPVKIRKSKSEEFIFNKDEFPRSGVTVEKLANLRPAFKKDG
TVTAGNSSGINDGAAALVLMSKEKANALNIKPMATLRGNATAGVDSNFMGIGPVPATNKALKKANISIQEIGLIEANEAF
ASQALAVQKELNINPDILNIHGGAISLGHPIGASGARILVTLLHAMKMQSTQFGLATLCIGGGMGAASIVELEK
>Mature_394_residues
MKEVVIVSAVRTATGSFMGLLSDIPATKLGSIVMKEALHRANVKPEEVEDVIFGNVLQAGLGQGTARQAAIQAGIPEYVP
ATTINKLCGSGLKSVHLAAQAIQTGDADIVLAGGMENMSQAPYLLEKGRQGFRMGDGKVVDSMIKDGLWCAINDVHMGIT
AENIADKYQISRKEQDKFAAWSQQKTEKAIEQGAFSDEIVPVKIRKSKSEEFIFNKDEFPRSGVTVEKLANLRPAFKKDG
TVTAGNSSGINDGAAALVLMSKEKANALNIKPMATLRGNATAGVDSNFMGIGPVPATNKALKKANISIQEIGLIEANEAF
ASQALAVQKELNINPDILNIHGGAISLGHPIGASGARILVTLLHAMKMQSTQFGLATLCIGGGMGAASIVELEK

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=52.0408163265306, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI167614485, Length=396, Percent_Identity=43.4343434343434, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=45.0381679389313, Blast_Score=326, Evalue=3e-89,
Organism=Homo sapiens, GI4501853, Length=399, Percent_Identity=41.3533834586466, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI4504327, Length=426, Percent_Identity=32.3943661971831, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=30.690537084399, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=57.9081632653061, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI87082165, Length=394, Percent_Identity=52.7918781725888, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1787663, Length=403, Percent_Identity=42.1836228287841, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=42.964824120603, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=29.2682926829268, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=45.2685421994885, Blast_Score=323, Evalue=9e-89,
Organism=Caenorhabditis elegans, GI25147385, Length=390, Percent_Identity=41.025641025641, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17535921, Length=392, Percent_Identity=39.2857142857143, Blast_Score=267, Evalue=8e-72,
Organism=Caenorhabditis elegans, GI17535917, Length=396, Percent_Identity=32.8282828282828, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=426, Percent_Identity=30.0469483568075, Blast_Score=178, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6325229, Length=397, Percent_Identity=42.8211586901763, Blast_Score=295, Evalue=7e-81,
Organism=Saccharomyces cerevisiae, GI6322031, Length=397, Percent_Identity=39.7984886649874, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=53.4526854219949, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24640423, Length=390, Percent_Identity=44.8717948717949, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI17648125, Length=396, Percent_Identity=43.4343434343434, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=31.0588235294118, Blast_Score=171, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41535; Mature: 41535

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVVIVSAVRTATGSFMGLLSDIPATKLGSIVMKEALHRANVKPEEVEDVIFGNVLQAG
CCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LGQGTARQAAIQAGIPEYVPATTINKLCGSGLKSVHLAAQAIQTGDADIVLAGGMENMSQ
CCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC
APYLLEKGRQGFRMGDGKVVDSMIKDGLWCAINDVHMGITAENIADKYQISRKEQDKFAA
CCHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECEEECEEHHHHHHHHHHHHHHHHHHHH
WSQQKTEKAIEQGAFSDEIVPVKIRKSKSEEFIFNKDEFPRSGVTVEKLANLRPAFKKDG
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCCHHHHHCCCCCCCCCC
TVTAGNSSGINDGAAALVLMSKEKANALNIKPMATLRGNATAGVDSNFMGIGPVPATNKA
CEECCCCCCCCCCCEEEEEEECCCCCEECCCEEEEECCCCCCCCCCCCCCCCCCCCCHHH
LKKANISIQEIGLIEANEAFASQALAVQKELNINPDILNIHGGAISLGHPIGASGARILV
HHHCCCCHHHCCEEECCHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCHHHHHH
TLLHAMKMQSTQFGLATLCIGGGMGAASIVELEK
HHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECC
>Mature Secondary Structure
MKEVVIVSAVRTATGSFMGLLSDIPATKLGSIVMKEALHRANVKPEEVEDVIFGNVLQAG
CCCEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LGQGTARQAAIQAGIPEYVPATTINKLCGSGLKSVHLAAQAIQTGDADIVLAGGMENMSQ
CCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC
APYLLEKGRQGFRMGDGKVVDSMIKDGLWCAINDVHMGITAENIADKYQISRKEQDKFAA
CCHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECEEECEEHHHHHHHHHHHHHHHHHHHH
WSQQKTEKAIEQGAFSDEIVPVKIRKSKSEEFIFNKDEFPRSGVTVEKLANLRPAFKKDG
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCCHHHHHCCCCCCCCCC
TVTAGNSSGINDGAAALVLMSKEKANALNIKPMATLRGNATAGVDSNFMGIGPVPATNKA
CEECCCCCCCCCCCEEEEEEECCCCCEECCCEEEEECCCCCCCCCCCCCCCCCCCCCHHH
LKKANISIQEIGLIEANEAFASQALAVQKELNINPDILNIHGGAISLGHPIGASGARILV
HHHCCCCHHHCCEEECCHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCHHHHHH
TLLHAMKMQSTQFGLATLCIGGGMGAASIVELEK
HHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]