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The map label for this gene is rbsB [H]

Identifier: 23100027

GI number: 23100027

Start: 2630693

End: 2631604

Strand: Reverse

Name: rbsB [H]

Synonym: OB2572

Alternate gene names: 23100027

Gene position: 2631604-2630693 (Counterclockwise)

Preceding gene: 23100028

Following gene: 23100026

Centisome position: 72.49

GC content: 38.82

Gene sequence:

>912_bases
ATGAAAAAATTCCAGATTGTATTCATAGCTATAATTATTATTTTTTTAGCAGCATGTTCAATGGAAAGTCCGTTTGTAAA
TAATGATTCTGATGACGACGATAATATAAAAATTGGTCTGAGTATTTCCACAATGGAAAACCCTTTCTTTGTAACGTTAC
ATGATACTATTGTGAAAGAAGCAAAGGAACAAGATATGGATATAGTAACTGTAAATGCGCAAGATGATGCTTCCGTTGAA
ATCAGCGGGATAGAAGACTTGATTCAACAACAGGTAGATATACTTCTTATAAACCCAACCGATTCTGCGGCTGTTTCATC
AGCGGTACAGTCTGCTAATGCTGCAGGTATTCCTGTTATTACTATTGACCGTTCAGCGGATGAAGGAGAAGTAGAAACAT
TAATTACTTCGGATAATATATCAGGCGGAGAAATGGCAGCAGAATTTATCGCCGACACAGTGGGAGAGAATGCGAATGTA
GTAGAGCTGCAAGGTATTGCGGGAGCTTCAGCTACAAGGGAACGCGGTGAAGGATTCCATAATATTGCTGAAGAACGTCT
GAATGTATTAGTATCTCAACCAGCAAATTTTGATCGGACAGAGGGATTAACCGTGATGGAGAATACATTACAAGGTTATA
GTGGTATTGATGCTGTATTTGCGCATAACGATGAAATGGCTTTAGGAGCTATTGAAGCTATTAAAGCTTCTGGTGAGGAT
ATTGTTGTTGTTGGTTTTGATGGAGTAGATGATGCTATTGCAGCTGTTGAGGCAGGAGAACTAACTGCAACGATTGCACA
ACAACCAAACCTAATGGGAGAAGAAGCAATAGCTACAGTTAAAGCCGTATTGAATGGCAACGAAGTGGACGAAGTAATTC
CTGTTCCATTAGAGCTAAAAACAAAGGAATAA

Upstream 100 bases:

>100_bases
TTACTTGGAATTTCCTCTTTCTATCAACAAGTAGTAAAAGGTGTTGTTATCTTAATCGCAGTGCTACTAGATCGTAAAAA
ATCCTAATGAGGTGAATGTA

Downstream 100 bases:

>100_bases
TTTTTAAACAAAAGTTTAGTGGGTAAGGTCATCGACGGATGACTTTGCCCACTATTTTTATGGTCTTAGAAAACCCCTGG
CTATGCCAGGGGTTCCAGAA

Product: ribose ABC transporter ribose-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKKFQIVFIAIIIIFLAACSMESPFVNNDSDDDDNIKIGLSISTMENPFFVTLHDTIVKEAKEQDMDIVTVNAQDDASVE
ISGIEDLIQQQVDILLINPTDSAAVSSAVQSANAAGIPVITIDRSADEGEVETLITSDNISGGEMAAEFIADTVGENANV
VELQGIAGASATRERGEGFHNIAEERLNVLVSQPANFDRTEGLTVMENTLQGYSGIDAVFAHNDEMALGAIEAIKASGED
IVVVGFDGVDDAIAAVEAGELTATIAQQPNLMGEEAIATVKAVLNGNEVDEVIPVPLELKTKE

Sequences:

>Translated_303_residues
MKKFQIVFIAIIIIFLAACSMESPFVNNDSDDDDNIKIGLSISTMENPFFVTLHDTIVKEAKEQDMDIVTVNAQDDASVE
ISGIEDLIQQQVDILLINPTDSAAVSSAVQSANAAGIPVITIDRSADEGEVETLITSDNISGGEMAAEFIADTVGENANV
VELQGIAGASATRERGEGFHNIAEERLNVLVSQPANFDRTEGLTVMENTLQGYSGIDAVFAHNDEMALGAIEAIKASGED
IVVVGFDGVDDAIAAVEAGELTATIAQQPNLMGEEAIATVKAVLNGNEVDEVIPVPLELKTKE
>Mature_303_residues
MKKFQIVFIAIIIIFLAACSMESPFVNNDSDDDDNIKIGLSISTMENPFFVTLHDTIVKEAKEQDMDIVTVNAQDDASVE
ISGIEDLIQQQVDILLINPTDSAAVSSAVQSANAAGIPVITIDRSADEGEVETLITSDNISGGEMAAEFIADTVGENANV
VELQGIAGASATRERGEGFHNIAEERLNVLVSQPANFDRTEGLTVMENTLQGYSGIDAVFAHNDEMALGAIEAIKASGED
IVVVGFDGVDDAIAAVEAGELTATIAQQPNLMGEEAIATVKAVLNGNEVDEVIPVPLELKTKE

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=268, Percent_Identity=50.3731343283582, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1790526, Length=270, Percent_Identity=31.4814814814815, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790674, Length=227, Percent_Identity=33.0396475770925, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790194, Length=248, Percent_Identity=26.6129032258064, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788473, Length=257, Percent_Identity=26.0700389105058, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32065; Mature: 32065

Theoretical pI: Translated: 3.67; Mature: 3.67

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFQIVFIAIIIIFLAACSMESPFVNNDSDDDDNIKIGLSISTMENPFFVTLHDTIVKE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEHHHHHHH
AKEQDMDIVTVNAQDDASVEISGIEDLIQQQVDILLINPTDSAAVSSAVQSANAAGIPVI
HHHCCCEEEEEECCCCCCEEECCHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCEEE
TIDRSADEGEVETLITSDNISGGEMAAEFIADTVGENANVVELQGIAGASATRERGEGFH
EEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCHH
NIAEERLNVLVSQPANFDRTEGLTVMENTLQGYSGIDAVFAHNDEMALGAIEAIKASGED
HHHHHHHHEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCE
IVVVGFDGVDDAIAAVEAGELTATIAQQPNLMGEEAIATVKAVLNGNEVDEVIPVPLELK
EEEEECCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEECCCEEEE
TKE
CCC
>Mature Secondary Structure
MKKFQIVFIAIIIIFLAACSMESPFVNNDSDDDDNIKIGLSISTMENPFFVTLHDTIVKE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEHHHHHHH
AKEQDMDIVTVNAQDDASVEISGIEDLIQQQVDILLINPTDSAAVSSAVQSANAAGIPVI
HHHCCCEEEEEECCCCCCEEECCHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCEEE
TIDRSADEGEVETLITSDNISGGEMAAEFIADTVGENANVVELQGIAGASATRERGEGFH
EEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCHH
NIAEERLNVLVSQPANFDRTEGLTVMENTLQGYSGIDAVFAHNDEMALGAIEAIKASGED
HHHHHHHHEEECCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCCE
IVVVGFDGVDDAIAAVEAGELTATIAQQPNLMGEEAIATVKAVLNGNEVDEVIPVPLELK
EEEEECCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEECCCEEEE
TKE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]