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The map label for this gene is gfo [H]

Identifier: 23100020

GI number: 23100020

Start: 2623364

End: 2624395

Strand: Reverse

Name: gfo [H]

Synonym: OB2565

Alternate gene names: 23100020

Gene position: 2624395-2623364 (Counterclockwise)

Preceding gene: 23100021

Following gene: 23100019

Centisome position: 72.29

GC content: 38.86

Gene sequence:

>1032_bases
ATGGTGAAAGTACGTGTAGCAGTAATTGGGTGTGGGAGCATTGCAAAAAATCGTCATTTAAAAGAATATAGCGCTAATCA
TCAAGTAGATATTGTAGCAGTTTGTGACATTGTTGAAGGTAGGGCAAGACAAGCAGCAGAATTACATAATGCCAAAGCCT
ATACGGATTATAAACAGTTATTAAAAGAAGAAGAAGTTGATGCAGTGAGTGTATGTTTACCAAACTATTTACACGCTCCT
GTAAGTATTGCTGCTCTAGAAGCAGGTTGTCACGTTTTATGTGAAAAACCGATGGCCACTTCAAAAGAAGAAGCGGAAGA
AATGATCCAGGCAGCGGAGAAAAATAATCGCAAACTAATGATTGGTCATAACCAACGCTTTGTTGCATCTCATCAGAAAG
CCAAACAGCTTATTGAAAGTGGAGAACTAGGAAAAATCTATAGTTTCCGGACAACTTTCGGTCATGGTGGCCCTGAAGGC
TGGAGTGCAGATGGAGCTGATAGTTGGTTTTTTAAGAAAGAGAAAGCTTTTGTGGGTGCAATGGGTGATTTAGGTGTGCA
CAAAGCAGATTTAATGCGTTATTTATTGAATGAAGAGTTTGTAGAGGTAGCAGCTTTTGTTGAAACAAGCGCAAAACAAA
CAGCAACCGTGGATGACAATGCCACGTTAGTCATGAAATCAGAAAGTGGCATTATCGGTACATTGGCAGCTAGTTGGGCT
TATAATGCTGCAGAGGATAATTCAACTGTTATATTTGCTGAAAATGCAATAGTTCGCTTAGAAGATGATCCAAACTATTC
GATGATTGTACAGTACACAAATGGTGAAGTAGTAAAGTATGAACTTGGTGGGATTCAATCGAATGAAGAAGGCGGACAAA
CGACTACGCATGTAATTGATCACTTTATTGAGTCAGTTACTACTAATCAACAACCTCTTATTGATGGATACGAAGGAATG
AAGTCGCTTAACGTAATTTTAGCAGCATTGTCTTCTGTAGAGACGAAGCAAATTTTTCAAATGGAGAAGTGA

Upstream 100 bases:

>100_bases
AATGCTGTTAAATGGGCGAAAAATACAGATACACCAGTTCATCAATATGGAAATGCACAACCTTTAGAAGATATTAAAAA
GTAGTAATGGAGGGGATAAA

Downstream 100 bases:

>100_bases
AAACGATGACTAGGTTAAAAATGGGGATTATTGGTGTTGGAGGCATTGCAGAGAGTAGACACATACCTGCTTATCTTTAT
CTTAATGAAATAGTTGAAGT

Product: NADH-dependent dyhydrogenase

Products: NA

Alternate protein names: GFOR [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MVKVRVAVIGCGSIAKNRHLKEYSANHQVDIVAVCDIVEGRARQAAELHNAKAYTDYKQLLKEEEVDAVSVCLPNYLHAP
VSIAALEAGCHVLCEKPMATSKEEAEEMIQAAEKNNRKLMIGHNQRFVASHQKAKQLIESGELGKIYSFRTTFGHGGPEG
WSADGADSWFFKKEKAFVGAMGDLGVHKADLMRYLLNEEFVEVAAFVETSAKQTATVDDNATLVMKSESGIIGTLAASWA
YNAAEDNSTVIFAENAIVRLEDDPNYSMIVQYTNGEVVKYELGGIQSNEEGGQTTTHVIDHFIESVTTNQQPLIDGYEGM
KSLNVILAALSSVETKQIFQMEK

Sequences:

>Translated_343_residues
MVKVRVAVIGCGSIAKNRHLKEYSANHQVDIVAVCDIVEGRARQAAELHNAKAYTDYKQLLKEEEVDAVSVCLPNYLHAP
VSIAALEAGCHVLCEKPMATSKEEAEEMIQAAEKNNRKLMIGHNQRFVASHQKAKQLIESGELGKIYSFRTTFGHGGPEG
WSADGADSWFFKKEKAFVGAMGDLGVHKADLMRYLLNEEFVEVAAFVETSAKQTATVDDNATLVMKSESGIIGTLAASWA
YNAAEDNSTVIFAENAIVRLEDDPNYSMIVQYTNGEVVKYELGGIQSNEEGGQTTTHVIDHFIESVTTNQQPLIDGYEGM
KSLNVILAALSSVETKQIFQMEK
>Mature_343_residues
MVKVRVAVIGCGSIAKNRHLKEYSANHQVDIVAVCDIVEGRARQAAELHNAKAYTDYKQLLKEEEVDAVSVCLPNYLHAP
VSIAALEAGCHVLCEKPMATSKEEAEEMIQAAEKNNRKLMIGHNQRFVASHQKAKQLIESGELGKIYSFRTTFGHGGPEG
WSADGADSWFFKKEKAFVGAMGDLGVHKADLMRYLLNEEFVEVAAFVETSAKQTATVDDNATLVMKSESGIIGTLAASWA
YNAAEDNSTVIFAENAIVRLEDDPNYSMIVQYTNGEVVKYELGGIQSNEEGGQTTTHVIDHFIESVTTNQQPLIDGYEGM
KSLNVILAALSSVETKQIFQMEK

Specific function: Unknown

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI1787574, Length=196, Percent_Identity=35.7142857142857, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082405, Length=189, Percent_Identity=30.1587301587302, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081947, Length=156, Percent_Identity=26.9230769230769, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789848, Length=220, Percent_Identity=28.6363636363636, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323975, Length=308, Percent_Identity=25, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24581117, Length=177, Percent_Identity=32.7683615819209, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008354
- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.99.28 [H]

Molecular weight: Translated: 37641; Mature: 37641

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKVRVAVIGCGSIAKNRHLKEYSANHQVDIVAVCDIVEGRARQAAELHNAKAYTDYKQL
CEEEEEEEEECCCHHHCCCHHHHCCCCCEEEEEEEEHHCCHHHHHHHHHCCHHHHHHHHH
LKEEEVDAVSVCLPNYLHAPVSIAALEAGCHVLCEKPMATSKEEAEEMIQAAEKNNRKLM
HHHHCCCCHHHHHCHHHCCCHHEEHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEE
IGHNQRFVASHQKAKQLIESGELGKIYSFRTTFGHGGPEGWSADGADSWFFKKEKAFVGA
EECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHEEC
MGDLGVHKADLMRYLLNEEFVEVAAFVETSAKQTATVDDNATLVMKSESGIIGTLAASWA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCEEEEEHHHHC
YNAAEDNSTVIFAENAIVRLEDDPNYSMIVQYTNGEVVKYELGGIQSNEEGGQTTTHVID
CCCCCCCCEEEEECCEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHH
HFIESVTTNQQPLIDGYEGMKSLNVILAALSSVETKQIFQMEK
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure
MVKVRVAVIGCGSIAKNRHLKEYSANHQVDIVAVCDIVEGRARQAAELHNAKAYTDYKQL
CEEEEEEEEECCCHHHCCCHHHHCCCCCEEEEEEEEHHCCHHHHHHHHHCCHHHHHHHHH
LKEEEVDAVSVCLPNYLHAPVSIAALEAGCHVLCEKPMATSKEEAEEMIQAAEKNNRKLM
HHHHCCCCHHHHHCHHHCCCHHEEHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEE
IGHNQRFVASHQKAKQLIESGELGKIYSFRTTFGHGGPEGWSADGADSWFFKKEKAFVGA
EECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHEEC
MGDLGVHKADLMRYLLNEEFVEVAAFVETSAKQTATVDDNATLVMKSESGIIGTLAASWA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCEEEEEHHHHC
YNAAEDNSTVIFAENAIVRLEDDPNYSMIVQYTNGEVVKYELGGIQSNEEGGQTTTHVID
CCCCCCCCEEEEECCEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHH
HFIESVTTNQQPLIDGYEGMKSLNVILAALSSVETKQIFQMEK
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1537789; 8472911; 8994968 [H]