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The map label for this gene is glpF [H]

Identifier: 23099931

GI number: 23099931

Start: 2533769

End: 2534599

Strand: Reverse

Name: glpF [H]

Synonym: OB2476

Alternate gene names: 23099931

Gene position: 2534599-2533769 (Counterclockwise)

Preceding gene: 23099932

Following gene: 23099930

Centisome position: 69.81

GC content: 39.23

Gene sequence:

>831_bases
ATGTCGGAGTTTATAGCTGAGTTAGTAGGCACAATGATTCTTATCATCTTCGGTGGCGGCGTTGTAGCAGGCGTTGTATT
AAAGAAATCGAAGGCAGAAGGTACAGGTTGGGTACTTATTACTCTTGGTTGGGGATTAGCAGTTGCTCTGGGTGTATATG
CTGTAGGTAATTTTACAGGAGCACACATAAATCCAGCGGTAACTATTGGATTTGCAGCTGTAGGAGATTTCCCTTGGAGT
AAAGTTCCACTATATATTCTTGCACAAGTAATCGGTGCCATTATTGGTGCAATTATAGTTTTCCTTAATTACTTACCGCA
CTGGAAAGTGACAGAAAGTAAAGAAGATAAATTAGGTGTATTTGCTACTGGACCAGCAATTCGTAGTCCATTTTCAAATT
TAATTAGTGAAATGATTGGTACATTTGCATTGCTTTTAGGATTGATGTTTATTGGATCGAACGATTTTACAGAAGGTTTA
AACCCGCTAATTGTCGGGGCACTTATTGCAGCTATTGGGATGTCTCTAGGTGGTACAACTGGTTATGCCATTAATCCAGC
ACGTGACCTTGGGCCTCGTATCGCACATGCAATTTTACCGATTCCTGGAAAAGGAAGTTCTGATTGGGGTTATGCGTGGA
TTCCAGTAGTAGGTCCAATTATTGGTGGAGTCTACGGTGCATTATTTTATGACAGTGTTTTTCTAGCTAATTATACCTTC
CCGTTTTGGGTAATGACTATTTTTGTAATAGTATTATTTATAGGAGCAGCAGTGAGTGAGTTGAAAACGGGTTCAAATCC
TGCTGAAAAAATGAATCAAAAATTAAGATAG

Upstream 100 bases:

>100_bases
GACCTACATTTCAATCGTACTCGCATATTTGGGTATGCTATTGAAGTGTGGGTTTTTTTAATACCACAAAGGGTACTTGA
TTAAAAGGGAGGTAGATGAT

Downstream 100 bases:

>100_bases
GAGGAATTTGGCATGTCAGAAAAATTTATTTTGTCCATTGATCAAGGAACAACAAGCTCTCGAGCAATACTTTTTAATCA
TAAGGGTGAGATCGTTGAAT

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSEFIAELVGTMILIIFGGGVVAGVVLKKSKAEGTGWVLITLGWGLAVALGVYAVGNFTGAHINPAVTIGFAAVGDFPWS
KVPLYILAQVIGAIIGAIIVFLNYLPHWKVTESKEDKLGVFATGPAIRSPFSNLISEMIGTFALLLGLMFIGSNDFTEGL
NPLIVGALIAAIGMSLGGTTGYAINPARDLGPRIAHAILPIPGKGSSDWGYAWIPVVGPIIGGVYGALFYDSVFLANYTF
PFWVMTIFVIVLFIGAAVSELKTGSNPAEKMNQKLR

Sequences:

>Translated_276_residues
MSEFIAELVGTMILIIFGGGVVAGVVLKKSKAEGTGWVLITLGWGLAVALGVYAVGNFTGAHINPAVTIGFAAVGDFPWS
KVPLYILAQVIGAIIGAIIVFLNYLPHWKVTESKEDKLGVFATGPAIRSPFSNLISEMIGTFALLLGLMFIGSNDFTEGL
NPLIVGALIAAIGMSLGGTTGYAINPARDLGPRIAHAILPIPGKGSSDWGYAWIPVVGPIIGGVYGALFYDSVFLANYTF
PFWVMTIFVIVLFIGAAVSELKTGSNPAEKMNQKLR
>Mature_275_residues
SEFIAELVGTMILIIFGGGVVAGVVLKKSKAEGTGWVLITLGWGLAVALGVYAVGNFTGAHINPAVTIGFAAVGDFPWSK
VPLYILAQVIGAIIGAIIVFLNYLPHWKVTESKEDKLGVFATGPAIRSPFSNLISEMIGTFALLLGLMFIGSNDFTEGLN
PLIVGALIAAIGMSLGGTTGYAINPARDLGPRIAHAILPIPGKGSSDWGYAWIPVVGPIIGGVYGALFYDSVFLANYTFP
FWVMTIFVIVLFIGAAVSELKTGSNPAEKMNQKLR

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=251, Percent_Identity=36.6533864541833, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI157266307, Length=255, Percent_Identity=34.1176470588235, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI4502187, Length=245, Percent_Identity=35.1020408163265, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI22538420, Length=243, Percent_Identity=33.7448559670782, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4755125, Length=210, Percent_Identity=33.8095238095238, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4502181, Length=210, Percent_Identity=32.8571428571429, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI310133356, Length=219, Percent_Identity=32.4200913242009, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI310114181, Length=219, Percent_Identity=32.4200913242009, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI37694062, Length=198, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4502179, Length=234, Percent_Identity=29.4871794871795, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI6912506, Length=234, Percent_Identity=29.9145299145299, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1790362, Length=252, Percent_Identity=37.6984126984127, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787101, Length=192, Percent_Identity=30.2083333333333, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71994009, Length=283, Percent_Identity=30.7420494699647, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17544068, Length=243, Percent_Identity=35.8024691358025, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17531431, Length=236, Percent_Identity=32.6271186440678, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17531429, Length=236, Percent_Identity=32.6271186440678, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71992966, Length=244, Percent_Identity=30.7377049180328, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17533613, Length=244, Percent_Identity=29.0983606557377, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32564052, Length=244, Percent_Identity=29.0983606557377, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71992961, Length=234, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17558372, Length=245, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71993722, Length=191, Percent_Identity=29.8429319371728, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321054, Length=216, Percent_Identity=39.8148148148148, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322985, Length=306, Percent_Identity=31.6993464052288, Blast_Score=108, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24652747, Length=235, Percent_Identity=32.3404255319149, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45551084, Length=235, Percent_Identity=32.3404255319149, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29049; Mature: 28917

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFIAELVGTMILIIFGGGVVAGVVLKKSKAEGTGWVLITLGWGLAVALGVYAVGNFTG
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCC
AHINPAVTIGFAAVGDFPWSKVPLYILAQVIGAIIGAIIVFLNYLPHWKVTESKEDKLGV
CCCCCHHEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
FATGPAIRSPFSNLISEMIGTFALLLGLMFIGSNDFTEGLNPLIVGALIAAIGMSLGGTT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHAILPIPGKGSSDWGYAWIPVVGPIIGGVYGALFYDSVFLANYTF
CCEECCHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCH
PFWVMTIFVIVLFIGAAVSELKTGSNPAEKMNQKLR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
SEFIAELVGTMILIIFGGGVVAGVVLKKSKAEGTGWVLITLGWGLAVALGVYAVGNFTG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCC
AHINPAVTIGFAAVGDFPWSKVPLYILAQVIGAIIGAIIVFLNYLPHWKVTESKEDKLGV
CCCCCHHEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
FATGPAIRSPFSNLISEMIGTFALLLGLMFIGSNDFTEGLNPLIVGALIAAIGMSLGGTT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHAILPIPGKGSSDWGYAWIPVVGPIIGGVYGALFYDSVFLANYTF
CCEECCHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCH
PFWVMTIFVIVLFIGAAVSELKTGSNPAEKMNQKLR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]