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The map label for this gene is citZ [H]

Identifier: 23099623

GI number: 23099623

Start: 2202373

End: 2203494

Strand: Reverse

Name: citZ [H]

Synonym: OB2168

Alternate gene names: 23099623

Gene position: 2203494-2202373 (Counterclockwise)

Preceding gene: 23099625

Following gene: 23099622

Centisome position: 60.69

GC content: 35.12

Gene sequence:

>1122_bases
TTGACAGCATCGACAAAGGGGTTAGAAGGTATTGTCGCTACGCAGTCATCGATTAGTTCGATTATTGATGATCAACTATC
CTATGTAGGGTATAGAATTGATGATTTGGCTGAAAATTCAAGCTTTGAAGAGATTATTTATCTACTTTGGAATCAAAAAT
TACCGAATAAGTCTGAATTGGATGAATTAAAGTCTCAACTGGCTGAAAATATGGATATACCTGATGCAATTATTAATCAC
TTGCGTTCCTATGATTTGAATACGGTACATCCAATGGCTGCATTACGTACTGCAATATCATTGCTTGGACTATATGATAA
TGAAGCAGATGTTATGGATGAACAAGCTAACAAACGAAAAGCGGTTCGTATTCAAGCACAAATAGCAACAATCGTAACCA
CTTTTTCTCGTATTCGTAAAGGTCAAGAACCTGTAAAACCAAAAAAGGATCTTAGCTACGCAGAAAACTTTATATATATG
TTAAATGGTAAAGAACCAGAGGATATCGAAGTAGAAGCAATTAATAAAGCGCTCGTACTTCATGCCGATCATGAACTTAA
TGCATCCACCTTTACTGCTCGAGTATGTGTGGCTACATTATCGGATATTTATTCAGGGATCACTGCAGCAATTGGAGCAT
TAAAAGGTCCATTACACGGTGGTGCTAATGAAAATGTCATGAAAATGCTAACTGAAATTGGCGAAGAGGAAAATGCTATT
CCTTATATAAAAGAAAAATTAGCAAATAAAGATAAAATCATGGGTATGGGACATCGTGTATACCGTACAGGAGATCCTCG
TGCAAAATTTTTACGAGAGATGTCAAAAGAACTTACAAAATTAACTGGACAAGAAAAATGGTATAATATGTCAGTTAAAA
TAGAAGATTATATTAAAGAAAATAAAGGGCTTCCTGCAAATGTAGATTTTTACAGTGCATCAGTATATCATAGCTTGGGT
CTTGATCATGATATATTCACACCATTATTTGCTGTAAGTCGTACTTCAGGATGGATTGCTCATATTCTAGAACAATATGA
AAATAACCGTTTAATTCGTCCACGTGCAGAGTACACAGGTCCAGAATTACGTGACTATGTTTCTATTGAACAAAGAGGTT
AA

Upstream 100 bases:

>100_bases
CTAATGTGATAACATTAGAGTAAATAGCTTGTGATTGTCTTATTTTATAATTCGCATTCTTAGTAATGCTTGAATCATTT
TTAAAAAGGAGAGGGTTATT

Downstream 100 bases:

>100_bases
TATAGATAAACTAGAGAAGATACATTGCGAATAACATTTGGGAGGTATTATAAATGACACAAGCTGAAAAAATCGTAGTA
GAAAACGGAAAAATGAACGT

Product: citrate synthase

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTASTKGLEGIVATQSSISSIIDDQLSYVGYRIDDLAENSSFEEIIYLLWNQKLPNKSELDELKSQLAENMDIPDAIINH
LRSYDLNTVHPMAALRTAISLLGLYDNEADVMDEQANKRKAVRIQAQIATIVTTFSRIRKGQEPVKPKKDLSYAENFIYM
LNGKEPEDIEVEAINKALVLHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANENVMKMLTEIGEEENAI
PYIKEKLANKDKIMGMGHRVYRTGDPRAKFLREMSKELTKLTGQEKWYNMSVKIEDYIKENKGLPANVDFYSASVYHSLG
LDHDIFTPLFAVSRTSGWIAHILEQYENNRLIRPRAEYTGPELRDYVSIEQRG

Sequences:

>Translated_373_residues
MTASTKGLEGIVATQSSISSIIDDQLSYVGYRIDDLAENSSFEEIIYLLWNQKLPNKSELDELKSQLAENMDIPDAIINH
LRSYDLNTVHPMAALRTAISLLGLYDNEADVMDEQANKRKAVRIQAQIATIVTTFSRIRKGQEPVKPKKDLSYAENFIYM
LNGKEPEDIEVEAINKALVLHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANENVMKMLTEIGEEENAI
PYIKEKLANKDKIMGMGHRVYRTGDPRAKFLREMSKELTKLTGQEKWYNMSVKIEDYIKENKGLPANVDFYSASVYHSLG
LDHDIFTPLFAVSRTSGWIAHILEQYENNRLIRPRAEYTGPELRDYVSIEQRG
>Mature_372_residues
TASTKGLEGIVATQSSISSIIDDQLSYVGYRIDDLAENSSFEEIIYLLWNQKLPNKSELDELKSQLAENMDIPDAIINHL
RSYDLNTVHPMAALRTAISLLGLYDNEADVMDEQANKRKAVRIQAQIATIVTTFSRIRKGQEPVKPKKDLSYAENFIYML
NGKEPEDIEVEAINKALVLHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANENVMKMLTEIGEEENAIP
YIKEKLANKDKIMGMGHRVYRTGDPRAKFLREMSKELTKLTGQEKWYNMSVKIEDYIKENKGLPANVDFYSASVYHSLGL
DHDIFTPLFAVSRTSGWIAHILEQYENNRLIRPRAEYTGPELRDYVSIEQRG

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=377, Percent_Identity=25.4641909814324, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=35.5978260869565, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1786939, Length=384, Percent_Identity=34.1145833333333, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17555174, Length=377, Percent_Identity=26.2599469496021, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319850, Length=327, Percent_Identity=25.9938837920489, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324328, Length=325, Percent_Identity=25.8461538461538, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640126, Length=328, Percent_Identity=27.4390243902439, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24640124, Length=328, Percent_Identity=27.4390243902439, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21356863, Length=335, Percent_Identity=27.1641791044776, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 41993; Mature: 41862

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASTKGLEGIVATQSSISSIIDDQLSYVGYRIDDLAENSSFEEIIYLLWNQKLPNKSEL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCCCCHHHH
DELKSQLAENMDIPDAIINHLRSYDLNTVHPMAALRTAISLLGLYDNEADVMDEQANKRK
HHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHH
AVRIQAQIATIVTTFSRIRKGQEPVKPKKDLSYAENFIYMLNGKEPEDIEVEAINKALVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCEECCCEEEE
HADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANENVMKMLTEIGEEENAI
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
PYIKEKLANKDKIMGMGHRVYRTGDPRAKFLREMSKELTKLTGQEKWYNMSVKIEDYIKE
HHHHHHHCCHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCHHEEEEEEHHHHHHH
NKGLPANVDFYSASVYHSLGLDHDIFTPLFAVSRTSGWIAHILEQYENNRLIRPRAEYTG
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEECCCHHCCC
PELRDYVSIEQRG
CHHHHHHCCCCCC
>Mature Secondary Structure 
TASTKGLEGIVATQSSISSIIDDQLSYVGYRIDDLAENSSFEEIIYLLWNQKLPNKSEL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCCCCHHHH
DELKSQLAENMDIPDAIINHLRSYDLNTVHPMAALRTAISLLGLYDNEADVMDEQANKRK
HHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHH
AVRIQAQIATIVTTFSRIRKGQEPVKPKKDLSYAENFIYMLNGKEPEDIEVEAINKALVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCEECCCEEEE
HADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANENVMKMLTEIGEEENAI
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
PYIKEKLANKDKIMGMGHRVYRTGDPRAKFLREMSKELTKLTGQEKWYNMSVKIEDYIKE
HHHHHHHCCHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCHHEEEEEEHHHHHHH
NKGLPANVDFYSASVYHSLGLDHDIFTPLFAVSRTSGWIAHILEQYENNRLIRPRAEYTG
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEECCCHHCCC
PELRDYVSIEQRG
CHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]