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The map label for this gene is citH

Identifier: 23099621

GI number: 23099621

Start: 2200000

End: 2200938

Strand: Reverse

Name: citH

Synonym: OB2166

Alternate gene names: 23099621

Gene position: 2200938-2200000 (Counterclockwise)

Preceding gene: 23099622

Following gene: 23099620

Centisome position: 60.62

GC content: 37.06

Gene sequence:

>939_bases
ATGGGTTTAAAACGTAAAAAAATATCAGTAATTGGTTCAGGTTTCACTGGTGCAACTACTGCTTTAATGGTGGCGCAAAA
AGAATTAGGCGATGTTGTATTAGTTGATATACCTGACATGGAAGACCCTACAAAAGGTAAAGCTCTGGATATGGCAGAAG
CAGCTCCTGTTCAAGGGTTTGATGCAAAAATTACTGGTACATCTAATTATGCTGATACAGAAGGTTCTGATCTCGTTATC
ATTACGGCTGGAATCGCTCGTAAGCCAGGAATGAGTCGTGATGATTTAGTTAATACGAATGCTAATATAATGAAATCCGT
TACCAAAGAAATTGTACATTACTCACCAAATACAACAATTGTTGTATTAACTAATCCAGTGGATGCGATGACATATACAG
TATTTAAAGAGTCTGGGTTACCAAAAGAGCGTGTGATTGGACAATCTGGAATTTTAGATACTGCACGTTTCCGTACATTT
GTAGCAGAGGAGCTTAATCTTTCTGTCAAAGATGTAACTGGCTTTGTTTTAGGTGGTCATGGAGACGATATGGTTCCACT
TATTCGTTATTCTTATGCTGGTGGAATTCCATTAGAAAAATTAATTCCTCAAGAACGTTTAGATGCAATTGTCCAACGTA
CTCGTACTGGTGGAGGAGAAATTGTTAATCTATTAGGTAATGGTAGTGCCTATTATGCACCTGCAGCTTCTTTAACTGTT
ATGGCTGAAGCAATTCTAAAAGATCAACGCCGCGTGTTACCTACTATTGCATATCTTGAAGGTGAATATGGATATCAAGA
TATCTATTTAGGCGTTCCAACTATCTTAGGCGGAGAAGGAATTGAAGAAATTATTGAGTTAGACTTAACGAAAGAAGAAA
AGGCACAACTTGATAAGTCTGCTGACTCTGTTAAAAATGTACTAAATGTACTTCAATAA

Upstream 100 bases:

>100_bases
AATGTGCTGTAGGGATAAGAACTAGCTATTCACTTAGAATACTTTCTTCATTCCTACGCCTCCATTTGAAATTAGATTTT
ACGATAAAGGAGATTTTTCG

Downstream 100 bases:

>100_bases
ACAAGTAAACTGCTCTATATGTCTATTAGTAGATGTATAGGGCAGTTTTTATCTGTTTCCAACAGTTCTTCCATCGATTC
GAATATTTACTAAATTAATT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MGLKRKKISVIGSGFTGATTALMVAQKELGDVVLVDIPDMEDPTKGKALDMAEAAPVQGFDAKITGTSNYADTEGSDLVI
ITAGIARKPGMSRDDLVNTNANIMKSVTKEIVHYSPNTTIVVLTNPVDAMTYTVFKESGLPKERVIGQSGILDTARFRTF
VAEELNLSVKDVTGFVLGGHGDDMVPLIRYSYAGGIPLEKLIPQERLDAIVQRTRTGGGEIVNLLGNGSAYYAPAASLTV
MAEAILKDQRRVLPTIAYLEGEYGYQDIYLGVPTILGGEGIEEIIELDLTKEEKAQLDKSADSVKNVLNVLQ

Sequences:

>Translated_312_residues
MGLKRKKISVIGSGFTGATTALMVAQKELGDVVLVDIPDMEDPTKGKALDMAEAAPVQGFDAKITGTSNYADTEGSDLVI
ITAGIARKPGMSRDDLVNTNANIMKSVTKEIVHYSPNTTIVVLTNPVDAMTYTVFKESGLPKERVIGQSGILDTARFRTF
VAEELNLSVKDVTGFVLGGHGDDMVPLIRYSYAGGIPLEKLIPQERLDAIVQRTRTGGGEIVNLLGNGSAYYAPAASLTV
MAEAILKDQRRVLPTIAYLEGEYGYQDIYLGVPTILGGEGIEEIIELDLTKEEKAQLDKSADSVKNVLNVLQ
>Mature_311_residues
GLKRKKISVIGSGFTGATTALMVAQKELGDVVLVDIPDMEDPTKGKALDMAEAAPVQGFDAKITGTSNYADTEGSDLVII
TAGIARKPGMSRDDLVNTNANIMKSVTKEIVHYSPNTTIVVLTNPVDAMTYTVFKESGLPKERVIGQSGILDTARFRTFV
AEELNLSVKDVTGFVLGGHGDDMVPLIRYSYAGGIPLEKLIPQERLDAIVQRTRTGGGEIVNLLGNGSAYYAPAASLTVM
AEAILKDQRRVLPTIAYLEGEYGYQDIYLGVPTILGGEGIEEIIELDLTKEEKAQLDKSADSVKNVLNVLQ

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=317, Percent_Identity=37.8548895899054, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI221136809, Length=317, Percent_Identity=37.8548895899054, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI260099723, Length=317, Percent_Identity=37.5394321766561, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI5031857, Length=317, Percent_Identity=37.5394321766561, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI15082234, Length=319, Percent_Identity=34.7962382445141, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI9257228, Length=312, Percent_Identity=36.2179487179487, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI4504973, Length=312, Percent_Identity=36.2179487179487, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI291575128, Length=310, Percent_Identity=34.5161290322581, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI4557032, Length=310, Percent_Identity=34.5161290322581, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=40, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI260099727, Length=200, Percent_Identity=40, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI207028494, Length=199, Percent_Identity=36.6834170854271, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI21735621, Length=335, Percent_Identity=31.3432835820896, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI103472011, Length=309, Percent_Identity=23.9482200647249, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI103472015, Length=180, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1789632, Length=313, Percent_Identity=31.3099041533546, Blast_Score=104, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17535107, Length=300, Percent_Identity=32, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17554310, Length=295, Percent_Identity=31.1864406779661, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=292, Percent_Identity=28.7671232876712, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=211, Percent_Identity=30.8056872037915, Blast_Score=73, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324446, Length=180, Percent_Identity=31.1111111111111, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17136226, Length=311, Percent_Identity=31.8327974276527, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45550422, Length=307, Percent_Identity=30.9446254071661, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24647881, Length=312, Percent_Identity=30.4487179487179, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24663599, Length=308, Percent_Identity=30.5194805194805, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24663595, Length=284, Percent_Identity=27.4647887323944, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_OCEIH (Q8EPE2)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693087.1
- ProteinModelPortal:   Q8EPE2
- SMR:   Q8EPE2
- GeneID:   1015599
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2166
- NMPDR:   fig|221109.1.peg.2164
- HOGENOM:   HBG566126
- OMA:   AMTYTVF
- ProtClustDB:   PRK06223
- BioCyc:   OIHE221109:OB2166-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33496; Mature: 33365

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: ACT_SITE 180-180 BINDING 36-36 BINDING 87-87 BINDING 93-93 BINDING 100-100 BINDING 125-125 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKRKKISVIGSGFTGATTALMVAQKELGDVVLVDIPDMEDPTKGKALDMAEAAPVQGF
CCCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEECHHHCCCCCCC
DAKITGTSNYADTEGSDLVIITAGIARKPGMSRDDLVNTNANIMKSVTKEIVHYSPNTTI
CEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHEECCCCEE
VVLTNPVDAMTYTVFKESGLPKERVIGQSGILDTARFRTFVAEELNLSVKDVTGFVLGGH
EEEECCCCHHEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCEEEHEEEEEEECC
GDDMVPLIRYSYAGGIPLEKLIPQERLDAIVQRTRTGGGEIVNLLGNGSAYYAPAASLTV
CCCCHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCEEECCHHHHHH
MAEAILKDQRRVLPTIAYLEGEYGYQDIYLGVPTILGGEGIEEIIELDLTKEEKAQLDKS
HHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCHHCCCCCHHHHHHHCCCHHHHHHHHHH
ADSVKNVLNVLQ
HHHHHHHHHHHC
>Mature Secondary Structure 
GLKRKKISVIGSGFTGATTALMVAQKELGDVVLVDIPDMEDPTKGKALDMAEAAPVQGF
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEECHHHCCCCCCC
DAKITGTSNYADTEGSDLVIITAGIARKPGMSRDDLVNTNANIMKSVTKEIVHYSPNTTI
CEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHEECCCCEE
VVLTNPVDAMTYTVFKESGLPKERVIGQSGILDTARFRTFVAEELNLSVKDVTGFVLGGH
EEEECCCCHHEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCEEEHEEEEEEECC
GDDMVPLIRYSYAGGIPLEKLIPQERLDAIVQRTRTGGGEIVNLLGNGSAYYAPAASLTV
CCCCHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCEEECCHHHHHH
MAEAILKDQRRVLPTIAYLEGEYGYQDIYLGVPTILGGEGIEEIIELDLTKEEKAQLDKS
HHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCHHCCCCCHHHHHHHCCCHHHHHHHHHH
ADSVKNVLNVLQ
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376