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The map label for this gene is phoR [H]

Identifier: 23099619

GI number: 23099619

Start: 2197645

End: 2199006

Strand: Reverse

Name: phoR [H]

Synonym: OB2164

Alternate gene names: 23099619

Gene position: 2199006-2197645 (Counterclockwise)

Preceding gene: 23099620

Following gene: 23099618

Centisome position: 60.57

GC content: 32.31

Gene sequence:

>1362_bases
ATGAAAGTAATCTTTTCTAGAACTTTACTTCCAAGTATGATTATTACTATATTGAGCTTATTATTGACTGGTTGGCTGAT
CGTTCAAGCTGAGTCTAATTGGATAATGGTTATTGCTGTATTAATTGTTCAATTTATGATTATCTCGATATTATTTATAC
AATTTTATAATCGTTATACTCGTCCGATTGGTAAAGCCACAAAAACAGTCGATCGGCTTATCGAAGGAAATTATAGCGCT
CGATTTTATAACGGTAATAGTCCTGAGATGGAAGAATTAAGTGTCAAAGTAAATACCCTAGCAAGAAATATGAGTGAAAT
TGCAATACAAGAACAGATGCAATCCGAACAATTAACAACAATTGTTGATAATATGCAAAGTGGACTAGTATTAATTGATG
AAAAAGGTTATGTTCATGTTGTAAATCGAAAATTTCTTGAGATGTTTGGTGAGGAAGAAAGTAATTATAGAGGACATCTT
TATTATGATGTGTTTGAAAATGAAAATGTACATGAAACTGTCCAAAAAACATTTTTGTATGAAGAAACTGTTAAAAAATC
ATTTGTGCATCGAGAAGGATTAAACAAAATTTATGTGGAAGTGGTCGGCGCACCGATTTTTAATGAAAGAAATATGTTAA
AGGGTGCAGTACTCGTACTTTATGATATCACTGAACTAAAGAAATTAGAAAAAATGCGTAAAGATTTTGTAGCGAATGTA
TCTCATGAACTTCGTACACCAATTACTTCTATTCGTGGTTTTGCTGAAACTTTATTAGACAACAATATCACGGATCCGGC
TACGAAGGAGTTTATGGAAATTATTTATAAGGAAAGTCATCGATTACAATTGCTAATTGAAGATTTACTTGCTCTTTCAA
GGTTAGAAAGGGAAGATTTTCGACTATTAATAGATAATTATGATGTAAGACAAATGGTAGAAGAAATTTTACCACAACTT
CACCAAAAGGCAGAAAATAAAAATCTAACATTCGATTTAGAGGTTCCAGATCAACTGACAATGCGAGCGGATAAAGATCG
TATGAAACAAGTATTAATCAATTTGATAGATAATTCTATTCATTATACACCTAGTGGTGGAGATATCTGCTTAGCTATTT
CAGAAGAAACGGATGTAATACATTTTCAAGTAAAAGATAGTGGAATCGGAATGGATGAAAAATCACAAACGAGAGTTTTT
GAGCGATTTTATAGAGTAGATAAAGCCAGAAGTAGAAATACGGGAGGAACCGGTCTAGGACTGGCGATTGTAAAGCATAT
TGTTGAAGTCCATAAAGGGAAAATAGATGTAGAGAGTACCTTGAACCAAGGAACTACGATTCATATCTATATACCAAAGT
AA

Upstream 100 bases:

>100_bases
ATATTAGTCATCTTCGAGATAAAATAGAACCTAATTCTAAAAAGCCTGTATATATCAAGACAGTACGCGGCTTGGGCTAT
AAAATGGAGGAGCCTGTTTC

Downstream 100 bases:

>100_bases
ATGAAGAAGAGTTGACGTCGCAACAGCCGTCAGCTCTTCTTTGTTATTCGTTAGGTAATAAATGCATGAAATTCACGTAT
TTTTTCCTTTTCTTGAATGA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKVIFSRTLLPSMIITILSLLLTGWLIVQAESNWIMVIAVLIVQFMIISILFIQFYNRYTRPIGKATKTVDRLIEGNYSA
RFYNGNSPEMEELSVKVNTLARNMSEIAIQEQMQSEQLTTIVDNMQSGLVLIDEKGYVHVVNRKFLEMFGEEESNYRGHL
YYDVFENENVHETVQKTFLYEETVKKSFVHREGLNKIYVEVVGAPIFNERNMLKGAVLVLYDITELKKLEKMRKDFVANV
SHELRTPITSIRGFAETLLDNNITDPATKEFMEIIYKESHRLQLLIEDLLALSRLEREDFRLLIDNYDVRQMVEEILPQL
HQKAENKNLTFDLEVPDQLTMRADKDRMKQVLINLIDNSIHYTPSGGDICLAISEETDVIHFQVKDSGIGMDEKSQTRVF
ERFYRVDKARSRNTGGTGLGLAIVKHIVEVHKGKIDVESTLNQGTTIHIYIPK

Sequences:

>Translated_453_residues
MKVIFSRTLLPSMIITILSLLLTGWLIVQAESNWIMVIAVLIVQFMIISILFIQFYNRYTRPIGKATKTVDRLIEGNYSA
RFYNGNSPEMEELSVKVNTLARNMSEIAIQEQMQSEQLTTIVDNMQSGLVLIDEKGYVHVVNRKFLEMFGEEESNYRGHL
YYDVFENENVHETVQKTFLYEETVKKSFVHREGLNKIYVEVVGAPIFNERNMLKGAVLVLYDITELKKLEKMRKDFVANV
SHELRTPITSIRGFAETLLDNNITDPATKEFMEIIYKESHRLQLLIEDLLALSRLEREDFRLLIDNYDVRQMVEEILPQL
HQKAENKNLTFDLEVPDQLTMRADKDRMKQVLINLIDNSIHYTPSGGDICLAISEETDVIHFQVKDSGIGMDEKSQTRVF
ERFYRVDKARSRNTGGTGLGLAIVKHIVEVHKGKIDVESTLNQGTTIHIYIPK
>Mature_453_residues
MKVIFSRTLLPSMIITILSLLLTGWLIVQAESNWIMVIAVLIVQFMIISILFIQFYNRYTRPIGKATKTVDRLIEGNYSA
RFYNGNSPEMEELSVKVNTLARNMSEIAIQEQMQSEQLTTIVDNMQSGLVLIDEKGYVHVVNRKFLEMFGEEESNYRGHL
YYDVFENENVHETVQKTFLYEETVKKSFVHREGLNKIYVEVVGAPIFNERNMLKGAVLVLYDITELKKLEKMRKDFVANV
SHELRTPITSIRGFAETLLDNNITDPATKEFMEIIYKESHRLQLLIEDLLALSRLEREDFRLLIDNYDVRQMVEEILPQL
HQKAENKNLTFDLEVPDQLTMRADKDRMKQVLINLIDNSIHYTPSGGDICLAISEETDVIHFQVKDSGIGMDEKSQTRVF
ERFYRVDKARSRNTGGTGLGLAIVKHIVEVHKGKIDVESTLNQGTTIHIYIPK

Specific function: Member of the two-component regulatory system phoP/phoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates phoP in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=240, Percent_Identity=35.8333333333333, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788393, Length=229, Percent_Identity=32.3144104803493, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788549, Length=378, Percent_Identity=25.6613756613757, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=33.1877729257642, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI48994928, Length=379, Percent_Identity=26.6490765171504, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1790346, Length=232, Percent_Identity=30.1724137931034, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693157, Length=255, Percent_Identity=29.0196078431373, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1788713, Length=226, Percent_Identity=29.2035398230088, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=29.4372294372294, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1790861, Length=236, Percent_Identity=29.2372881355932, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786783, Length=223, Percent_Identity=29.1479820627803, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI87082128, Length=236, Percent_Identity=27.9661016949153, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI87081816, Length=235, Percent_Identity=26.3829787234043, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=29.2576419213974, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788279, Length=229, Percent_Identity=27.9475982532751, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787894, Length=220, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789808, Length=230, Percent_Identity=26.9565217391304, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790551, Length=204, Percent_Identity=29.9019607843137, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790300, Length=241, Percent_Identity=26.9709543568465, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789403, Length=197, Percent_Identity=24.8730964467005, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52334; Mature: 52334

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50885 HAMP ; PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIFSRTLLPSMIITILSLLLTGWLIVQAESNWIMVIAVLIVQFMIISILFIQFYNRYT
CCEEEHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPIGKATKTVDRLIEGNYSARFYNGNSPEMEELSVKVNTLARNMSEIAIQEQMQSEQLTT
CHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVDNMQSGLVLIDEKGYVHVVNRKFLEMFGEEESNYRGHLYYDVFENENVHETVQKTFLY
HHHHHCCCEEEEECCCEEEEHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
EETVKKSFVHREGLNKIYVEVVGAPIFNERNMLKGAVLVLYDITELKKLEKMRKDFVANV
HHHHHHHHHHHHCCHHEEHHEECCCCCCCCCHHHHHHEEEEEHHHHHHHHHHHHHHHHHH
SHELRTPITSIRGFAETLLDNNITDPATKEFMEIIYKESHRLQLLIEDLLALSRLEREDF
HHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHH
RLLIDNYDVRQMVEEILPQLHQKAENKNLTFDLEVPDQLTMRADKDRMKQVLINLIDNSI
EEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHCCCC
HYTPSGGDICLAISEETDVIHFQVKDSGIGMDEKSQTRVFERFYRVDKARSRNTGGTGLG
EECCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
LAIVKHIVEVHKGKIDVESTLNQGTTIHIYIPK
HHHHHHHHHHHCCCCCHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MKVIFSRTLLPSMIITILSLLLTGWLIVQAESNWIMVIAVLIVQFMIISILFIQFYNRYT
CCEEEHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPIGKATKTVDRLIEGNYSARFYNGNSPEMEELSVKVNTLARNMSEIAIQEQMQSEQLTT
CHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVDNMQSGLVLIDEKGYVHVVNRKFLEMFGEEESNYRGHLYYDVFENENVHETVQKTFLY
HHHHHCCCEEEEECCCEEEEHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
EETVKKSFVHREGLNKIYVEVVGAPIFNERNMLKGAVLVLYDITELKKLEKMRKDFVANV
HHHHHHHHHHHHCCHHEEHHEECCCCCCCCCHHHHHHEEEEEHHHHHHHHHHHHHHHHHH
SHELRTPITSIRGFAETLLDNNITDPATKEFMEIIYKESHRLQLLIEDLLALSRLEREDF
HHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHH
RLLIDNYDVRQMVEEILPQLHQKAENKNLTFDLEVPDQLTMRADKDRMKQVLINLIDNSI
EEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHCCCC
HYTPSGGDICLAISEETDVIHFQVKDSGIGMDEKSQTRVFERFYRVDKARSRNTGGTGLG
EECCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
LAIVKHIVEVHKGKIDVESTLNQGTTIHIYIPK
HHHHHHHHHHHCCCCCHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3142862; 9387221; 9384377 [H]