Click here to switch to the map view.

The map label for this gene is murI

Identifier: 23099563

GI number: 23099563

Start: 2142511

End: 2143335

Strand: Reverse

Name: murI

Synonym: OB2108

Alternate gene names: 23099563

Gene position: 2143335-2142511 (Counterclockwise)

Preceding gene: 23099564

Following gene: 23099562

Centisome position: 59.04

GC content: 38.06

Gene sequence:

>825_bases
GTGGACAAATCGATTGGGGTAATTGATTCGGGGGTAGGTGGACTTACAGTTGTTCACGAACTCATGAGGCAGCTTCCAAA
GGAGCAATTAATTTATTTAGGCGATACAGCTAGATGTCCTTATGGCCCGAGATCAAAGGAAGAGGTAAGGCAATTCACAT
GGGAAATGGTGGATTTCTTATTAACAAAAAATATAAAAATGTTAGTTGTTGCATGTAATACGGCCACTGCATTTACATTA
AAAGATTTAAAAGAACAATTAGATATACCAGTTATCGGAGTTATCCAACCAGGTGCACGTGCTGCAATAAAATCTACAAC
AAATAACCAAGTCGGTGTAATCGGTACAGAAGGAACAATTAGTAGCGGAGCTTACTCACAAGCATTGGAAAAAATTAAGT
CAGACATTCAAGTAAGCGGATTAGCTTGTCCTCCATTTGTTCCAATGGTTGAGCGAGGGGTGTTATCTGGTCCTGAAGCA
AAAGCAGTTGTATCAGAGGCTTTACGACCATTCTCGAATAACAAAGAAATGGATACCTTAATATTGGGCTGTACCCATTA
CCCTTTACTTAAATCTACGATACAGGAGGTAATGGGGGAAGAAGTCACCGTTATTTCCTCTAGTGAAGAAACGGCACGTG
AAACTAGCACACTTCTAGATGTTCACCAAATTATGAACCGGAGTGATTTAGTTCCACTTCATCAATTTTATACAACTGGT
GATTTGGAAATTTTTATCGAAATTGCAAAAACAATATTTCAAGAATCTCACTTCCAAATGCTAACCATTAAACAAGCGAA
TATATCAACTAACAAAGTTTGGTAA

Upstream 100 bases:

>100_bases
GGTCACTGATGAGCTGGATGAGTGACCAAAATCGATGAGAGCGATAAAAAAAGGCACTCATTATAGTGGTCTAAATCTAA
ACGAAAGAGGAGACATCTTT

Downstream 100 bases:

>100_bases
AACAATGTTAAATGTATTGTTCGTATCAGAGAGTTCCAATTACCGATGTCGGAGATTGGAACTTTTAATTTTCATGGTAT
TTTTCTGCTTTCATGTAATT

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MDKSIGVIDSGVGGLTVVHELMRQLPKEQLIYLGDTARCPYGPRSKEEVRQFTWEMVDFLLTKNIKMLVVACNTATAFTL
KDLKEQLDIPVIGVIQPGARAAIKSTTNNQVGVIGTEGTISSGAYSQALEKIKSDIQVSGLACPPFVPMVERGVLSGPEA
KAVVSEALRPFSNNKEMDTLILGCTHYPLLKSTIQEVMGEEVTVISSSEETARETSTLLDVHQIMNRSDLVPLHQFYTTG
DLEIFIEIAKTIFQESHFQMLTIKQANISTNKVW

Sequences:

>Translated_274_residues
MDKSIGVIDSGVGGLTVVHELMRQLPKEQLIYLGDTARCPYGPRSKEEVRQFTWEMVDFLLTKNIKMLVVACNTATAFTL
KDLKEQLDIPVIGVIQPGARAAIKSTTNNQVGVIGTEGTISSGAYSQALEKIKSDIQVSGLACPPFVPMVERGVLSGPEA
KAVVSEALRPFSNNKEMDTLILGCTHYPLLKSTIQEVMGEEVTVISSSEETARETSTLLDVHQIMNRSDLVPLHQFYTTG
DLEIFIEIAKTIFQESHFQMLTIKQANISTNKVW
>Mature_274_residues
MDKSIGVIDSGVGGLTVVHELMRQLPKEQLIYLGDTARCPYGPRSKEEVRQFTWEMVDFLLTKNIKMLVVACNTATAFTL
KDLKEQLDIPVIGVIQPGARAAIKSTTNNQVGVIGTEGTISSGAYSQALEKIKSDIQVSGLACPPFVPMVERGVLSGPEA
KAVVSEALRPFSNNKEMDTLILGCTHYPLLKSTIQEVMGEEVTVISSSEETARETSTLLDVHQIMNRSDLVPLHQFYTTG
DLEIFIEIAKTIFQESHFQMLTIKQANISTNKVW

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=223, Percent_Identity=33.1838565022422, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_OCEIH (Q8EPJ4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693029.1
- ProteinModelPortal:   Q8EPJ4
- SMR:   Q8EPJ4
- GeneID:   1015722
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2108
- NMPDR:   fig|221109.1.peg.2106
- HOGENOM:   HBG645102
- OMA:   AILGCTH
- BioCyc:   OIHE221109:OB2108-MONOMER
- BRENDA:   5.1.1.3
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 30159; Mature: 30159

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSIGVIDSGVGGLTVVHELMRQLPKEQLIYLGDTARCPYGPRSKEEVRQFTWEMVDFL
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
LTKNIKMLVVACNTATAFTLKDLKEQLDIPVIGVIQPGARAAIKSTTNNQVGVIGTEGTI
HHCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCEEEEECCCCC
SSGAYSQALEKIKSDIQVSGLACPPFVPMVERGVLSGPEAKAVVSEALRPFSNNKEMDTL
CCCHHHHHHHHHHHCCEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHE
ILGCTHYPLLKSTIQEVMGEEVTVISSSEETARETSTLLDVHQIMNRSDLVPLHQFYTTG
EEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCC
DLEIFIEIAKTIFQESHFQMLTIKQANISTNKVW
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MDKSIGVIDSGVGGLTVVHELMRQLPKEQLIYLGDTARCPYGPRSKEEVRQFTWEMVDFL
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
LTKNIKMLVVACNTATAFTLKDLKEQLDIPVIGVIQPGARAAIKSTTNNQVGVIGTEGTI
HHCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCEEEEECCCCC
SSGAYSQALEKIKSDIQVSGLACPPFVPMVERGVLSGPEAKAVVSEALRPFSNNKEMDTL
CCCHHHHHHHHHHHCCEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHE
ILGCTHYPLLKSTIQEVMGEEVTVISSSEETARETSTLLDVHQIMNRSDLVPLHQFYTTG
EEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCC
DLEIFIEIAKTIFQESHFQMLTIKQANISTNKVW
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376