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The map label for this gene is pgl [H]

Identifier: 23099543

GI number: 23099543

Start: 2118778

End: 2120265

Strand: Reverse

Name: pgl [H]

Synonym: OB2088

Alternate gene names: 23099543

Gene position: 2120265-2118778 (Counterclockwise)

Preceding gene: 23099544

Following gene: 23099542

Centisome position: 58.4

GC content: 37.16

Gene sequence:

>1488_bases
ATGTTTTGTAATATTACTGAATTTGGAGCAAAGGGAGATTCCAACACAGACAATACTGAATCGATTTCATCAGCAATCAA
GCGATGTGCTGATAGTGGAGGAGGAACACTCTACATTCCAGCAGGAACGTACTTGACGGGGCCAATTTCGCTGATTAGTA
ATCTGACAATCTATATTGAATCAGGTGCAAAACTAGTTTTTATAGATGATTTCTCTGCTTACCCTCCAGTTAAAACAAGA
TGGTCTGGATATGAATGCTATGGATTTTCACCACTGTTATATGGTAATGGTTTAACTAATGTATCAATTATAGGTTCAGG
TTGTATTGATGGTCAGGGGGAAGCTTGGTGGGAGGTAAATCATCTATTAAGAAAAGGCGAAGAATATGATCACCCACAAA
CGAAGGAGATAGCTGAATTAAATAAATCTATAACTGAACCTAAAAATACGAATCTTGTCGAATGGCCATCACAATTTCTT
CGACCACCTTTACTTCAAATGTACGATTGTGATTCAGTGACATTGGATGGTATTACGCTGGAAAATTCACCATTTTGGAA
TACGCATTTTGTATATTGCGATAATGTCACTATTCACAATGTGAAGTTTAAAAATCCTTGGGATACTCCAAATGGGGATG
GACTAGATCTTGATTCATGTTCCAATGTGCGTGTATCAAACTGTCATTTTGATGTGGGCGACGATTGTTTAGCATTTAAA
TCTGGTATCAATGAAGATGGTAGGAGAGTTGCAAGACCAACGGAAAATGTGGCTGTTACAAACTGCACGATGAAAAATGG
GCATGGTGGAATTGTAATGGGAAGTGAGAATTCAGGTGGAATAAGAAATATTGCTGTATCAAACTGTGTGTTTATAGGCA
CAGATCGGGGCATTCGATTAAAAACGAATCGAGCTCGCGGGAGCTACATTCGTGATATTTTAATTGACAATATTTATATG
GATGGGGTTCTCTGTCCTTTGGCGATAAATTCCTTTTATCGGCACGGTTTGGATAAGAGTGATTCTTTAATTAATGAATT
GTCGCCAATAGAAATTTCAGAAAAGACTCCAGAAATCGAATATATTCATATAAGTAATGTAACAGCTAGAAACTGTCGCT
CAGCTGCAGGGTTTATTTACGGACTTCCTGAGAAGTATGTAAAAGATATTATTCTACGGCACGTATTAATTGAAATGACA
ATCAATCCCGATGAACAAGGTGGAGAACCAGATATGGTAAAAGAACCAATATTTATGGCCGGAGAAGGCATTTATTGTAA
GTACGTTGATGGAATAGAATTTCATCGTGTCAGAGTGGAAACAAGACAGGGGCCTGCTCTTCACTTGGAAAATGTTATTA
ATGCAGAATTAGATCATTTAATTAGGAAGCGTAAACACCCAAATACTCCTATAGTTACTGTCAATGACGTAACTAATATA
CGGACTAATCAATCTAGTAGGGAGGTAAATGAATTTGAAAACATTTAA

Upstream 100 bases:

>100_bases
TCCATTTTTACAAAAATACTTTGCAAAAGGAGCATTACTAGGTTCAGTAAAAGGTTAGTAATATCTGAGTAAATAACGAA
ATGAATGGAGGCATATGTAT

Downstream 100 bases:

>100_bases
AAACCCAATCATCCCTGGCTTTTACCCTGATCCATCTATCTGTCGAGTAGATGAGGATTATTATTTAGTAACTTCAACAT
TTGAATATTTCCCAGGAGTT

Product: hypothetical protein

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MFCNITEFGAKGDSNTDNTESISSAIKRCADSGGGTLYIPAGTYLTGPISLISNLTIYIESGAKLVFIDDFSAYPPVKTR
WSGYECYGFSPLLYGNGLTNVSIIGSGCIDGQGEAWWEVNHLLRKGEEYDHPQTKEIAELNKSITEPKNTNLVEWPSQFL
RPPLLQMYDCDSVTLDGITLENSPFWNTHFVYCDNVTIHNVKFKNPWDTPNGDGLDLDSCSNVRVSNCHFDVGDDCLAFK
SGINEDGRRVARPTENVAVTNCTMKNGHGGIVMGSENSGGIRNIAVSNCVFIGTDRGIRLKTNRARGSYIRDILIDNIYM
DGVLCPLAINSFYRHGLDKSDSLINELSPIEISEKTPEIEYIHISNVTARNCRSAAGFIYGLPEKYVKDIILRHVLIEMT
INPDEQGGEPDMVKEPIFMAGEGIYCKYVDGIEFHRVRVETRQGPALHLENVINAELDHLIRKRKHPNTPIVTVNDVTNI
RTNQSSREVNEFENI

Sequences:

>Translated_495_residues
MFCNITEFGAKGDSNTDNTESISSAIKRCADSGGGTLYIPAGTYLTGPISLISNLTIYIESGAKLVFIDDFSAYPPVKTR
WSGYECYGFSPLLYGNGLTNVSIIGSGCIDGQGEAWWEVNHLLRKGEEYDHPQTKEIAELNKSITEPKNTNLVEWPSQFL
RPPLLQMYDCDSVTLDGITLENSPFWNTHFVYCDNVTIHNVKFKNPWDTPNGDGLDLDSCSNVRVSNCHFDVGDDCLAFK
SGINEDGRRVARPTENVAVTNCTMKNGHGGIVMGSENSGGIRNIAVSNCVFIGTDRGIRLKTNRARGSYIRDILIDNIYM
DGVLCPLAINSFYRHGLDKSDSLINELSPIEISEKTPEIEYIHISNVTARNCRSAAGFIYGLPEKYVKDIILRHVLIEMT
INPDEQGGEPDMVKEPIFMAGEGIYCKYVDGIEFHRVRVETRQGPALHLENVINAELDHLIRKRKHPNTPIVTVNDVTNI
RTNQSSREVNEFENI
>Mature_495_residues
MFCNITEFGAKGDSNTDNTESISSAIKRCADSGGGTLYIPAGTYLTGPISLISNLTIYIESGAKLVFIDDFSAYPPVKTR
WSGYECYGFSPLLYGNGLTNVSIIGSGCIDGQGEAWWEVNHLLRKGEEYDHPQTKEIAELNKSITEPKNTNLVEWPSQFL
RPPLLQMYDCDSVTLDGITLENSPFWNTHFVYCDNVTIHNVKFKNPWDTPNGDGLDLDSCSNVRVSNCHFDVGDDCLAFK
SGINEDGRRVARPTENVAVTNCTMKNGHGGIVMGSENSGGIRNIAVSNCVFIGTDRGIRLKTNRARGSYIRDILIDNIYM
DGVLCPLAINSFYRHGLDKSDSLINELSPIEISEKTPEIEYIHISNVTARNCRSAAGFIYGLPEKYVKDIILRHVLIEMT
INPDEQGGEPDMVKEPIFMAGEGIYCKYVDGIEFHRVRVETRQGPALHLENVINAELDHLIRKRKHPNTPIVTVNDVTNI
RTNQSSREVNEFENI

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 55135; Mature: 55135

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFCNITEFGAKGDSNTDNTESISSAIKRCADSGGGTLYIPAGTYLTGPISLISNLTIYIE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEECCHHHHHCCEEEEEE
SGAKLVFIDDFSAYPPVKTRWSGYECYGFSPLLYGNGLTNVSIIGSGCIDGQGEAWWEVN
CCCEEEEEECCCCCCCCCCCCCCEEEECCCEEEECCCCCEEEEEECCCCCCCCCHHHHHH
HLLRKGEEYDHPQTKEIAELNKSITEPKNTNLVEWPSQFLRPPLLQMYDCDSVTLDGITL
HHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHCCCCHHHHCCCCCEEECCEEE
ENSPFWNTHFVYCDNVTIHNVKFKNPWDTPNGDGLDLDSCSNVRVSNCHFDVGDDCLAFK
CCCCCCCEEEEEECCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHH
SGINEDGRRVARPTENVAVTNCTMKNGHGGIVMGSENSGGIRNIAVSNCVFIGTDRGIRL
CCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCCEEEEEECCEEEEECCCCEEE
KTNRARGSYIRDILIDNIYMDGVLCPLAINSFYRHGLDKSDSLINELSPIEISEKTPEIE
EECCCCCHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCCCCHHHHHHCCCCEECCCCCCEE
YIHISNVTARNCRSAAGFIYGLPEKYVKDIILRHVLIEMTINPDEQGGEPDMVKEPIFMA
EEEECCCCHHHHHHHCCEEEECCHHHHHHHHHHHEEEEEEECCCCCCCCCCHHCCCCEEC
GEGIYCKYVDGIEFHRVRVETRQGPALHLENVINAELDHLIRKRKHPNTPIVTVNDVTNI
CCCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
RTNQSSREVNEFENI
CCCCCHHHHHHHHCC
>Mature Secondary Structure
MFCNITEFGAKGDSNTDNTESISSAIKRCADSGGGTLYIPAGTYLTGPISLISNLTIYIE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEECCHHHHHCCEEEEEE
SGAKLVFIDDFSAYPPVKTRWSGYECYGFSPLLYGNGLTNVSIIGSGCIDGQGEAWWEVN
CCCEEEEEECCCCCCCCCCCCCCEEEECCCEEEECCCCCEEEEEECCCCCCCCCHHHHHH
HLLRKGEEYDHPQTKEIAELNKSITEPKNTNLVEWPSQFLRPPLLQMYDCDSVTLDGITL
HHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHCCCCHHHHCCCCCEEECCEEE
ENSPFWNTHFVYCDNVTIHNVKFKNPWDTPNGDGLDLDSCSNVRVSNCHFDVGDDCLAFK
CCCCCCCEEEEEECCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHH
SGINEDGRRVARPTENVAVTNCTMKNGHGGIVMGSENSGGIRNIAVSNCVFIGTDRGIRL
CCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCCEEEEEECCEEEEECCCCEEE
KTNRARGSYIRDILIDNIYMDGVLCPLAINSFYRHGLDKSDSLINELSPIEISEKTPEIE
EECCCCCHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCCCCHHHHHHCCCCEECCCCCCEE
YIHISNVTARNCRSAAGFIYGLPEKYVKDIILRHVLIEMTINPDEQGGEPDMVKEPIFMA
EEEECCCCHHHHHHHCCEEEECCHHHHHHHHHHHEEEEEEECCCCCCCCCCHHCCCCEEC
GEGIYCKYVDGIEFHRVRVETRQGPALHLENVINAELDHLIRKRKHPNTPIVTVNDVTNI
CCCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
RTNQSSREVNEFENI
CCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]