Click here to switch to the map view.

The map label for this gene is tig

Identifier: 23099533

GI number: 23099533

Start: 2107214

End: 2108500

Strand: Reverse

Name: tig

Synonym: OB2078

Alternate gene names: 23099533

Gene position: 2108500-2107214 (Counterclockwise)

Preceding gene: 23099534

Following gene: 23099532

Centisome position: 58.08

GC content: 35.98

Gene sequence:

>1287_bases
ATGACAGCAAAATGGGAAAAACAAGAAGGTAATAAAGGTGTACTAACTTTTGAAGTCTCAGCTGAAGAGTTTGAGCAGGC
ATTAGATCAAGCCTTTAAAAAAGTATCTAAAGATGTTCAGATTCCAGGATTCCGTAAAGGGAAAATACCTCGCGGGATAT
TCGAAAAACGTTTTGGCGTAGAAGCACTTTATCAAGACGCGGTAGATATTGTATTACCATCTGCATATACAAAAGCAGTG
GAAGAAGCGGATATCTTCCCTATAGCTCAACCATCTGTGGATATTGATCAAATCGAAAGAGGTAAAGAATTAATCTTTAC
TGCTGAAGTTGAAGTGAAGCCAGAAGTGAAACTTGGCGAATACAAAGGCTTAGAAGTAGAAGAAGAATCAGTAGAAGTTA
CAGATGAAGATGTAGAAAAAGAGATTGAAAATGTACGTGAGCGTCATGCTGAACTAATTGTAAAAGAAGAAGAAGCTATT
GAAAATGGCGATACTGCTGTTATTGACTTTGAAGGTTTCCAAGATGGAGTTGCTTTTGAAGGCGGTAAAGGTGAAAATCA
TTCATTAGAAATTGGTTCTGGTCAATTCATTCCAGGATTTGAAGAGCAGCTTATTGGTAAGAAAGCAGGAGAAGAAACAG
AAGTTACTGTTACTTTCCCAGAAGAATACCATGCAGAAGATTTAGCTGGTAAAGAAGCTGTATTTAACGTAAAAATTAAT
GAAGTAAAAGCGAAAGAACTACCAGAACTTGATGATGAATTTGCAAAAGATGTCGATGAAGAAGTAGAATCATTAGATGA
GTTGAAAAAGAAAAAACGTAATGAGTTAGAAACAAATAAAAAACAAGAAGTTGAAAATAAAAAGCGTGAAGAACTAATCC
AAAAAGCTTCAGATAATGTTGAAGTAGATATTCCTGAAGCAATGGTTGATACAGAAGTAAACCAAATGGTTCGTGAGTTT
GAACAACAACTTCAGATGCAAGGTATGACATTAGAAATGTACGCACAGTTCTCTGGGCAAGATGAGGATGCTCTAAAAGA
ACAAATGCGTGAAGATGCTGCAAAGCGTGTGAAAACTAATCTAACATTAGAAGCTATTTCTGAAGCAGAAGGTATAAAAC
CTTCAGAAGAAGACATAAAAGCTGAACTTGAAAAAATGGCTTCTATGTACGGCGCGGAAGTTGATCAACTAGTACAAATG
CTAGGTGGAAACACAGAAACGCTAGAAAATGATTTGAAAGTTAAAGCTGCAATTGACTTCTTAGCAGAAAACAGCAAGAC
TAAATAA

Upstream 100 bases:

>100_bases
CCATTAGCATTTATGTTATAATGAGATGGTTGTAAATAGCGAACATTACATTTATGAAAAACAAAGAAATTTGTATTATA
GATTTTGGAGGGAATAAATA

Downstream 100 bases:

>100_bases
TGACAGTTGGATAGAAGACAAGGTGCGATTTTTGTCGCACCTTGTCTTTCATATTTCTAGTAATTATATGGATATTTACT
CACTGTTTAAACATATAGTG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTAKWEKQEGNKGVLTFEVSAEEFEQALDQAFKKVSKDVQIPGFRKGKIPRGIFEKRFGVEALYQDAVDIVLPSAYTKAV
EEADIFPIAQPSVDIDQIERGKELIFTAEVEVKPEVKLGEYKGLEVEEESVEVTDEDVEKEIENVRERHAELIVKEEEAI
ENGDTAVIDFEGFQDGVAFEGGKGENHSLEIGSGQFIPGFEEQLIGKKAGEETEVTVTFPEEYHAEDLAGKEAVFNVKIN
EVKAKELPELDDEFAKDVDEEVESLDELKKKKRNELETNKKQEVENKKREELIQKASDNVEVDIPEAMVDTEVNQMVREF
EQQLQMQGMTLEMYAQFSGQDEDALKEQMREDAAKRVKTNLTLEAISEAEGIKPSEEDIKAELEKMASMYGAEVDQLVQM
LGGNTETLENDLKVKAAIDFLAENSKTK

Sequences:

>Translated_428_residues
MTAKWEKQEGNKGVLTFEVSAEEFEQALDQAFKKVSKDVQIPGFRKGKIPRGIFEKRFGVEALYQDAVDIVLPSAYTKAV
EEADIFPIAQPSVDIDQIERGKELIFTAEVEVKPEVKLGEYKGLEVEEESVEVTDEDVEKEIENVRERHAELIVKEEEAI
ENGDTAVIDFEGFQDGVAFEGGKGENHSLEIGSGQFIPGFEEQLIGKKAGEETEVTVTFPEEYHAEDLAGKEAVFNVKIN
EVKAKELPELDDEFAKDVDEEVESLDELKKKKRNELETNKKQEVENKKREELIQKASDNVEVDIPEAMVDTEVNQMVREF
EQQLQMQGMTLEMYAQFSGQDEDALKEQMREDAAKRVKTNLTLEAISEAEGIKPSEEDIKAELEKMASMYGAEVDQLVQM
LGGNTETLENDLKVKAAIDFLAENSKTK
>Mature_427_residues
TAKWEKQEGNKGVLTFEVSAEEFEQALDQAFKKVSKDVQIPGFRKGKIPRGIFEKRFGVEALYQDAVDIVLPSAYTKAVE
EADIFPIAQPSVDIDQIERGKELIFTAEVEVKPEVKLGEYKGLEVEEESVEVTDEDVEKEIENVRERHAELIVKEEEAIE
NGDTAVIDFEGFQDGVAFEGGKGENHSLEIGSGQFIPGFEEQLIGKKAGEETEVTVTFPEEYHAEDLAGKEAVFNVKINE
VKAKELPELDDEFAKDVDEEVESLDELKKKKRNELETNKKQEVENKKREELIQKASDNVEVDIPEAMVDTEVNQMVREFE
QQLQMQGMTLEMYAQFSGQDEDALKEQMREDAAKRVKTNLTLEAISEAEGIKPSEEDIKAELEKMASMYGAEVDQLVQML
GGNTETLENDLKVKAAIDFLAENSKTK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=414, Percent_Identity=33.5748792270531, Blast_Score=214, Evalue=1e-56,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_OCEIH (Q8CXB7)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692999.1
- ProteinModelPortal:   Q8CXB7
- SMR:   Q8CXB7
- GeneID:   1017783
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2078
- NMPDR:   fig|221109.1.peg.2076
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   OIHE221109:OB2078-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48230; Mature: 48099

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKWEKQEGNKGVLTFEVSAEEFEQALDQAFKKVSKDVQIPGFRKGKIPRGIFEKRFGV
CCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
EALYQDAVDIVLPSAYTKAVEEADIFPIAQPSVDIDQIERGKELIFTAEVEVKPEVKLGE
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEEEEECCCCCCCC
YKGLEVEEESVEVTDEDVEKEIENVRERHAELIVKEEEAIENGDTAVIDFEGFQDGVAFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEC
GGKGENHSLEIGSGQFIPGFEEQLIGKKAGEETEVTVTFPEEYHAEDLAGKEAVFNVKIN
CCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCCEEEEECCHHHCHHHCCCCCEEEEEEEC
EVKAKELPELDDEFAKDVDEEVESLDELKKKKRNELETNKKQEVENKKREELIQKASDNV
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
EVDIPEAMVDTEVNQMVREFEQQLQMQGMTLEMYAQFSGQDEDALKEQMREDAAKRVKTN
EEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCHHHHHHHHHHHHHHHHHHC
LTLEAISEAEGIKPSEEDIKAELEKMASMYGAEVDQLVQMLGGNTETLENDLKVKAAIDF
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
LAENSKTK
HHCCCCCC
>Mature Secondary Structure 
TAKWEKQEGNKGVLTFEVSAEEFEQALDQAFKKVSKDVQIPGFRKGKIPRGIFEKRFGV
CCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
EALYQDAVDIVLPSAYTKAVEEADIFPIAQPSVDIDQIERGKELIFTAEVEVKPEVKLGE
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEEEEECCCCCCCC
YKGLEVEEESVEVTDEDVEKEIENVRERHAELIVKEEEAIENGDTAVIDFEGFQDGVAFE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEC
GGKGENHSLEIGSGQFIPGFEEQLIGKKAGEETEVTVTFPEEYHAEDLAGKEAVFNVKIN
CCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCCEEEEECCHHHCHHHCCCCCEEEEEEEC
EVKAKELPELDDEFAKDVDEEVESLDELKKKKRNELETNKKQEVENKKREELIQKASDNV
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
EVDIPEAMVDTEVNQMVREFEQQLQMQGMTLEMYAQFSGQDEDALKEQMREDAAKRVKTN
EEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCHHHHHHHHHHHHHHHHHHC
LTLEAISEAEGIKPSEEDIKAELEKMASMYGAEVDQLVQMLGGNTETLENDLKVKAAIDF
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
LAENSKTK
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376