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The map label for this gene is hemC

Identifier: 23099523

GI number: 23099523

Start: 2095233

End: 2096162

Strand: Reverse

Name: hemC

Synonym: OB2068

Alternate gene names: 23099523

Gene position: 2096162-2095233 (Counterclockwise)

Preceding gene: 23099524

Following gene: 23099522

Centisome position: 57.74

GC content: 38.06

Gene sequence:

>930_bases
ATGAGGAAAATTATTGTTGGTACAAGAAAAAGTAATCTTGCTTTAACTCAAACAGAGTGGGTTATCGATCAGTTGAAAAA
AGCTGGAGTAAAAAATGAATTTGAAATAAAGAAGATTGTTACAAAAGGAGATCAGATTCTAGATGTAACCCTATCTAAGG
TTGGCGGTAAGGGACTGTTTGTTAAAGAAATTGAAAAAGCAATGTTTGATAAAGAAATTGATTTAGCAGTACATAGTATG
AAAGATATGCCTGCGGTAATACCAGAAGGGCTTACAATTTCGTCTATTCCTGAGCGTGAAGATCATCGTGATGCGTACCT
TGCAAAAGACAATATTCTCTTGCAAGACCTTCCTGAAGGTGCCATCGTTGGTACAAGTAGTTTACGTCGAGGAGCACAGA
TTTTAGCAGAACGACCAGATTTGACTATAAAATGGATTCGAGGAAATATTGAAACTCGAATTCGAAAACTTCAAGAAGAA
GACTATGATGCAATTATTCTTGCGGTTTCTGGATTAAAAAGGGTAGGACTAAGTGAAGAACTAATAACAGAATATCTGGA
ACCAGAGGTGTGTGTTCCTGCGGTAGGACAAGGTGCACTGGCAATTGAAAGTAGAGAAGATGATGAAGAACTTACCAATA
TAGTTAAAAAAATTCATGATGCTTATACAGCGAAGACGGTAAGTGCAGAAAGAACGTTTCTTCATTTATTAGAAGGTGGA
TGCCAAGTTCCAATCGGCGGATATGCGTATTTAGACGGGGATGAGGTAGTATTAACTGCCTTAGTAGGAGATCCAGATGG
TACAACTATCTTAAAAGAAACAGTTCGTGGCACAGAACCCACTGAAGTTGGGAAAGAAGCTGCGGAGTTATTAATAAGTC
AAGGAGCAAAAGAAATCGTTGATCGGGTAAAAGAGGAGATGGATCAGTAA

Upstream 100 bases:

>100_bases
AGGGCATAGCTTTATATAATATTATTTCGTTTTTAGTATTATTAATTAATTTTTTCCTATCCAGTACATTATCAAACTTT
CATTACTAGGAGGATCTTAA

Downstream 100 bases:

>100_bases
TGAGTCACTCTCTTCAAGGAAAGAGTGTTTTGATTACTAGAGAAAAACAACAAGCAGAAGCTTTTGCTAAACAGATTGAA
AATTTTGGTGGTAAGCCACT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRKIIVGTRKSNLALTQTEWVIDQLKKAGVKNEFEIKKIVTKGDQILDVTLSKVGGKGLFVKEIEKAMFDKEIDLAVHSM
KDMPAVIPEGLTISSIPEREDHRDAYLAKDNILLQDLPEGAIVGTSSLRRGAQILAERPDLTIKWIRGNIETRIRKLQEE
DYDAIILAVSGLKRVGLSEELITEYLEPEVCVPAVGQGALAIESREDDEELTNIVKKIHDAYTAKTVSAERTFLHLLEGG
CQVPIGGYAYLDGDEVVLTALVGDPDGTTILKETVRGTEPTEVGKEAAELLISQGAKEIVDRVKEEMDQ

Sequences:

>Translated_309_residues
MRKIIVGTRKSNLALTQTEWVIDQLKKAGVKNEFEIKKIVTKGDQILDVTLSKVGGKGLFVKEIEKAMFDKEIDLAVHSM
KDMPAVIPEGLTISSIPEREDHRDAYLAKDNILLQDLPEGAIVGTSSLRRGAQILAERPDLTIKWIRGNIETRIRKLQEE
DYDAIILAVSGLKRVGLSEELITEYLEPEVCVPAVGQGALAIESREDDEELTNIVKKIHDAYTAKTVSAERTFLHLLEGG
CQVPIGGYAYLDGDEVVLTALVGDPDGTTILKETVRGTEPTEVGKEAAELLISQGAKEIVDRVKEEMDQ
>Mature_309_residues
MRKIIVGTRKSNLALTQTEWVIDQLKKAGVKNEFEIKKIVTKGDQILDVTLSKVGGKGLFVKEIEKAMFDKEIDLAVHSM
KDMPAVIPEGLTISSIPEREDHRDAYLAKDNILLQDLPEGAIVGTSSLRRGAQILAERPDLTIKWIRGNIETRIRKLQEE
DYDAIILAVSGLKRVGLSEELITEYLEPEVCVPAVGQGALAIESREDDEELTNIVKKIHDAYTAKTVSAERTFLHLLEGG
CQVPIGGYAYLDGDEVVLTALVGDPDGTTILKETVRGTEPTEVGKEAAELLISQGAKEIVDRVKEEMDQ

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=343, Percent_Identity=41.399416909621, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI66933009, Length=343, Percent_Identity=41.399416909621, Blast_Score=238, Evalue=6e-63,
Organism=Escherichia coli, GI48994974, Length=300, Percent_Identity=49.6666666666667, Blast_Score=270, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6319996, Length=318, Percent_Identity=38.3647798742138, Blast_Score=184, Evalue=1e-47,
Organism=Drosophila melanogaster, GI20130425, Length=258, Percent_Identity=43.4108527131783, Blast_Score=209, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_OCEIH (Q8CXC0)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692989.1
- ProteinModelPortal:   Q8CXC0
- SMR:   Q8CXC0
- GeneID:   1017796
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2068
- NMPDR:   fig|221109.1.peg.2066
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   OIHE221109:OB2068-MONOMER
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34058; Mature: 34058

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIIVGTRKSNLALTQTEWVIDQLKKAGVKNEFEIKKIVTKGDQILDVTLSKVGGKGLF
CCEEEEECCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEHHHHHCCCCCCH
VKEIEKAMFDKEIDLAVHSMKDMPAVIPEGLTISSIPEREDHRDAYLAKDNILLQDLPEG
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCC
AIVGTSSLRRGAQILAERPDLTIKWIRGNIETRIRKLQEEDYDAIILAVSGLKRVGLSEE
CEECCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEHHHHHCCCCHH
LITEYLEPEVCVPAVGQGALAIESREDDEELTNIVKKIHDAYTAKTVSAERTFLHLLEGG
HHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
CQVPIGGYAYLDGDEVVLTALVGDPDGTTILKETVRGTEPTEVGKEAAELLISQGAKEIV
CCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHH
DRVKEEMDQ
HHHHHHHCC
>Mature Secondary Structure
MRKIIVGTRKSNLALTQTEWVIDQLKKAGVKNEFEIKKIVTKGDQILDVTLSKVGGKGLF
CCEEEEECCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEHHHHHCCCCCCH
VKEIEKAMFDKEIDLAVHSMKDMPAVIPEGLTISSIPEREDHRDAYLAKDNILLQDLPEG
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCC
AIVGTSSLRRGAQILAERPDLTIKWIRGNIETRIRKLQEEDYDAIILAVSGLKRVGLSEE
CEECCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEHHHHHCCCCHH
LITEYLEPEVCVPAVGQGALAIESREDDEELTNIVKKIHDAYTAKTVSAERTFLHLLEGG
HHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
CQVPIGGYAYLDGDEVVLTALVGDPDGTTILKETVRGTEPTEVGKEAAELLISQGAKEIV
CCCCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHH
DRVKEEMDQ
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376