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The map label for this gene is queA

Identifier: 23099489

GI number: 23099489

Start: 2063456

End: 2064484

Strand: Reverse

Name: queA

Synonym: OB2034

Alternate gene names: 23099489

Gene position: 2064484-2063456 (Counterclockwise)

Preceding gene: 23099490

Following gene: 23099488

Centisome position: 56.86

GC content: 37.32

Gene sequence:

>1029_bases
ATGCAGATAGAAGATTTTGATTTTCATTTACCAGAAGAATTAATTGCACAAACACCGCTTAAGGATCGTACATCTTCACG
CTTATGTGTTTTGGATAAACAGACTAGCGAAATTACCCACCGTAGTTTCAAAGATATTACTACTTACCTTCAAGCAGGAG
ACTGTTTAGTGTTAAATGACACAAAAGTATTACCTGCAAGATTGTACGGCGTAAAATCAGATACAGGTGCAAAAATAGAG
GTATTACTTTTACATCAACAAGAAGAAGATACATGGGAAGTACTCGTGAAACCAGCAAAAAAAGTTAAAGTTGGCACGGA
AATCATATTTGGTAATGGAAAGTTAAAAGCGACTTGTATTGATCTAAAAGATCACGGTGGTCGAATCATGACTTTTTCTT
ATACAGGTATTTTTTATGAGATTTTAGACCAATTAGGTGAGATGCCATTACCTCCATATATTAAAGAACAATTACCAGAG
CAAGATAGATATCAAACTGTCTATGCGAAAGAGAAAGGTTCTGCAGCTGCACCTACTGCTGGGTTACACTTTACCGATAA
GTTGTTAGAAGATATCAAAGCTATGGGTGTCAATATTGTTTTTGTCACATTACATGTAGGACTTGGTACATTTCGACCAG
TCAGTGTTGATTCAGTTGAAGATCACGATATGCACTCGGAATTTTATTCCATGTCGGAATCTGCAGCAAAAACACTGAAT
GAAGCCAAGGCAGCTGGAAGAAGAATTATCTCTGTAGGCACCACATCTACGCGCACACTAGAAACGATTGTTCGCGACCA
TGATGGCAAGTTTGTTGCTACTAGAGGGTGGACAGATATTTTTATCTATCCTCCATATAAATTCCGCGCGATTGATGGTC
TAATCACAAATTTCCATTTACCGAAATCAACATTAATTATGATGATAAGTGCGTTAGCGGGTAAGGAAGCAATCCTTCAT
GCTTACAATGAAGCAGTGAAAGAAGAATATCGTTTCTTCAGCTTTGGTGACGCAATGTTAATCTTATAA

Upstream 100 bases:

>100_bases
TACATTTCATTTTCCAATTGTAACTTCAATTGTTGTTAGTATTATTTTGACATTGATTATGTTTATCATTGGAAGATTTC
GTTAAATGAAGGAGATAAAC

Downstream 100 bases:

>100_bases
AAAGGAAGAATATAATGAATCCAATAACGTATGAATTAATTAAGACGGATAAACAAACTGGTGCTAGATTAGGACGAGTT
CATACTCCTCATGGGTCGTT

Product: S-adenosylmethionine-tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MQIEDFDFHLPEELIAQTPLKDRTSSRLCVLDKQTSEITHRSFKDITTYLQAGDCLVLNDTKVLPARLYGVKSDTGAKIE
VLLLHQQEEDTWEVLVKPAKKVKVGTEIIFGNGKLKATCIDLKDHGGRIMTFSYTGIFYEILDQLGEMPLPPYIKEQLPE
QDRYQTVYAKEKGSAAAPTAGLHFTDKLLEDIKAMGVNIVFVTLHVGLGTFRPVSVDSVEDHDMHSEFYSMSESAAKTLN
EAKAAGRRIISVGTTSTRTLETIVRDHDGKFVATRGWTDIFIYPPYKFRAIDGLITNFHLPKSTLIMMISALAGKEAILH
AYNEAVKEEYRFFSFGDAMLIL

Sequences:

>Translated_342_residues
MQIEDFDFHLPEELIAQTPLKDRTSSRLCVLDKQTSEITHRSFKDITTYLQAGDCLVLNDTKVLPARLYGVKSDTGAKIE
VLLLHQQEEDTWEVLVKPAKKVKVGTEIIFGNGKLKATCIDLKDHGGRIMTFSYTGIFYEILDQLGEMPLPPYIKEQLPE
QDRYQTVYAKEKGSAAAPTAGLHFTDKLLEDIKAMGVNIVFVTLHVGLGTFRPVSVDSVEDHDMHSEFYSMSESAAKTLN
EAKAAGRRIISVGTTSTRTLETIVRDHDGKFVATRGWTDIFIYPPYKFRAIDGLITNFHLPKSTLIMMISALAGKEAILH
AYNEAVKEEYRFFSFGDAMLIL
>Mature_342_residues
MQIEDFDFHLPEELIAQTPLKDRTSSRLCVLDKQTSEITHRSFKDITTYLQAGDCLVLNDTKVLPARLYGVKSDTGAKIE
VLLLHQQEEDTWEVLVKPAKKVKVGTEIIFGNGKLKATCIDLKDHGGRIMTFSYTGIFYEILDQLGEMPLPPYIKEQLPE
QDRYQTVYAKEKGSAAAPTAGLHFTDKLLEDIKAMGVNIVFVTLHVGLGTFRPVSVDSVEDHDMHSEFYSMSESAAKTLN
EAKAAGRRIISVGTTSTRTLETIVRDHDGKFVATRGWTDIFIYPPYKFRAIDGLITNFHLPKSTLIMMISALAGKEAILH
AYNEAVKEEYRFFSFGDAMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=45.6647398843931, Blast_Score=303, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_OCEIH (Q8EPQ8)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692955.1
- ProteinModelPortal:   Q8EPQ8
- SMR:   Q8EPQ8
- GeneID:   1018523
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2034
- NMPDR:   fig|221109.1.peg.2032
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- BioCyc:   OIHE221109:OB2034-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38404; Mature: 38404

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIEDFDFHLPEELIAQTPLKDRTSSRLCVLDKQTSEITHRSFKDITTYLQAGDCLVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEC
TKVLPARLYGVKSDTGAKIEVLLLHQQEEDTWEVLVKPAKKVKVGTEIIFGNGKLKATCI
CCCCCHHEEECCCCCCCEEEEEEEECCCCCHHHHHHCCHHHEEECEEEEEECCEEEEEEE
DLKDHGGRIMTFSYTGIFYEILDQLGEMPLPPYIKEQLPEQDRYQTVYAKEKGSAAAPTA
EEECCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHEEEEEECCCCCCCCCC
GLHFTDKLLEDIKAMGVNIVFVTLHVGLGTFRPVSVDSVEDHDMHSEFYSMSESAAKTLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EAKAAGRRIISVGTTSTRTLETIVRDHDGKFVATRGWTDIFIYPPYKFRAIDGLITNFHL
HHHHCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCEEEEEECCCCEEEHHHHHHCCCC
PKSTLIMMISALAGKEAILHAYNEAVKEEYRFFSFGDAMLIL
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEECCEEEEC
>Mature Secondary Structure
MQIEDFDFHLPEELIAQTPLKDRTSSRLCVLDKQTSEITHRSFKDITTYLQAGDCLVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEC
TKVLPARLYGVKSDTGAKIEVLLLHQQEEDTWEVLVKPAKKVKVGTEIIFGNGKLKATCI
CCCCCHHEEECCCCCCCEEEEEEEECCCCCHHHHHHCCHHHEEECEEEEEECCEEEEEEE
DLKDHGGRIMTFSYTGIFYEILDQLGEMPLPPYIKEQLPEQDRYQTVYAKEKGSAAAPTA
EEECCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHEEEEEECCCCCCCCCC
GLHFTDKLLEDIKAMGVNIVFVTLHVGLGTFRPVSVDSVEDHDMHSEFYSMSESAAKTLN
CCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EAKAAGRRIISVGTTSTRTLETIVRDHDGKFVATRGWTDIFIYPPYKFRAIDGLITNFHL
HHHHCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCEEEEEECCCCEEEHHHHHHCCCC
PKSTLIMMISALAGKEAILHAYNEAVKEEYRFFSFGDAMLIL
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376