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The map label for this gene is tgt

Identifier: 23099488

GI number: 23099488

Start: 2062302

End: 2063441

Strand: Reverse

Name: tgt

Synonym: OB2033

Alternate gene names: 23099488

Gene position: 2063441-2062302 (Counterclockwise)

Preceding gene: 23099489

Following gene: 23099487

Centisome position: 56.84

GC content: 37.54

Gene sequence:

>1140_bases
ATGAATCCAATAACGTATGAATTAATTAAGACGGATAAACAAACTGGTGCTAGATTAGGACGAGTTCATACTCCTCATGG
GTCGTTTGATACGCCAACATTTATGCCAGTAGGGACGTTGGCAACAGTAAAAACGATGAGCCCAGAAGATTTGCAATCCA
TGCAAGCAAATATTATATTATCAAATACGTATCACCTTTGGTTACGGCCTGGTGAAGATATTATTCGAGAAGCTGGCGGA
TTGCATAAGTTTATGAATTGGGATGGAGCAATTCTTACTGATTCTGGTGGTTTTCAAGTATTTAGTCTGAGTGATATGCG
AGAGATAAAAGAAGAAGGAGTCCATTTTCGTAATCACTTAAATGGAGAGAAACTTTTTCTATCTCCAGAAAAAGCCATGG
ATATTCAAAATGCTCTTGGATCTGATATTATGATGGCCTTTGATGAATGTCCTCCATATCCAGCGGAATATGATTACATG
AAAGCCTCGGTAGAAAGAACTTCTCGCTGGGCGGAGAGATGTTTACAGGCGCATAATCGTCCTCATGATCAAGGTTTGTT
TGGAATAGTCCAAGGTGGGGAATATGAAGCGTTGCGAAAGCAAAGTGCGGAAGATTTAGTTTCATTAGATTTTCCTGGTT
ATGCAATTGGTGGACTTTCTGTTGGAGAGCCAAAGCATGTTATGAATAAAGTATTAGAATTTACAACTCCATTTTTACCA
TCTAATAAACCGCGTTATTTAATGGGAGTTGGCTCGCCAGATGCACTTATAGATGGGGCGATTCGCGGTGTAGATATGTT
TGACTGTGTATTACCAACTAGAATTGCTCGAAATGGAACATGCATGACATCAAACGGACGATTAGTAGTAAGAAATGCAA
AATATGCTCGAGATTTTAATCCGATAGATGAGCATTGTAGTTGTCATGTTTGTAAGAATTATTCAAGAGCGTACATCAGG
CATTTAATAAAATGTAATGAAACTTTTGGGTTCAGACTTACGACTTATCATAACTTGCATTTTCTGTTAAAATTAATGGA
GCAAGTTCGAACTGCTATAAAAGAAGATCGACTTGGTGATTTCAAAGAGTCATTCTTTGAACAATATGGATTAAACAAAG
CTAATCCAAAGAACTTTTAG

Upstream 100 bases:

>100_bases
AGGAAGCAATCCTTCATGCTTACAATGAAGCAGTGAAAGAAGAATATCGTTTCTTCAGCTTTGGTGACGCAATGTTAATC
TTATAAAAAGGAAGAATATA

Downstream 100 bases:

>100_bases
AAAGGAGTTGAATTAGATGGAAGCTATTATGCCATTTATGTGGATTATAATAATGTTTGCGATTTTTTATTTCTTACTTA
TTCGTCCACAACAAAAGCGT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MNPITYELIKTDKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEDLQSMQANIILSNTYHLWLRPGEDIIREAGG
LHKFMNWDGAILTDSGGFQVFSLSDMREIKEEGVHFRNHLNGEKLFLSPEKAMDIQNALGSDIMMAFDECPPYPAEYDYM
KASVERTSRWAERCLQAHNRPHDQGLFGIVQGGEYEALRKQSAEDLVSLDFPGYAIGGLSVGEPKHVMNKVLEFTTPFLP
SNKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTCMTSNGRLVVRNAKYARDFNPIDEHCSCHVCKNYSRAYIR
HLIKCNETFGFRLTTYHNLHFLLKLMEQVRTAIKEDRLGDFKESFFEQYGLNKANPKNF

Sequences:

>Translated_379_residues
MNPITYELIKTDKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEDLQSMQANIILSNTYHLWLRPGEDIIREAGG
LHKFMNWDGAILTDSGGFQVFSLSDMREIKEEGVHFRNHLNGEKLFLSPEKAMDIQNALGSDIMMAFDECPPYPAEYDYM
KASVERTSRWAERCLQAHNRPHDQGLFGIVQGGEYEALRKQSAEDLVSLDFPGYAIGGLSVGEPKHVMNKVLEFTTPFLP
SNKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTCMTSNGRLVVRNAKYARDFNPIDEHCSCHVCKNYSRAYIR
HLIKCNETFGFRLTTYHNLHFLLKLMEQVRTAIKEDRLGDFKESFFEQYGLNKANPKNF
>Mature_379_residues
MNPITYELIKTDKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEDLQSMQANIILSNTYHLWLRPGEDIIREAGG
LHKFMNWDGAILTDSGGFQVFSLSDMREIKEEGVHFRNHLNGEKLFLSPEKAMDIQNALGSDIMMAFDECPPYPAEYDYM
KASVERTSRWAERCLQAHNRPHDQGLFGIVQGGEYEALRKQSAEDLVSLDFPGYAIGGLSVGEPKHVMNKVLEFTTPFLP
SNKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTCMTSNGRLVVRNAKYARDFNPIDEHCSCHVCKNYSRAYIR
HLIKCNETFGFRLTTYHNLHFLLKLMEQVRTAIKEDRLGDFKESFFEQYGLNKANPKNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=369, Percent_Identity=44.7154471544715, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI13375872, Length=291, Percent_Identity=26.1168384879725, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786607, Length=368, Percent_Identity=53.5326086956522, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17544680, Length=363, Percent_Identity=47.1074380165289, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI25146502, Length=246, Percent_Identity=25.2032520325203, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920510, Length=374, Percent_Identity=43.3155080213904, Blast_Score=328, Evalue=5e-90,
Organism=Drosophila melanogaster, GI21355311, Length=267, Percent_Identity=23.2209737827715, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_OCEIH (Q8CXC4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692954.1
- ProteinModelPortal:   Q8CXC4
- SMR:   Q8CXC4
- GeneID:   1018522
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2033
- NMPDR:   fig|221109.1.peg.2031
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   OIHE221109:OB2033-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42916; Mature: 42916

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: ACT_SITE 94-94 BINDING 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPITYELIKTDKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEDLQSMQANIIL
CCCEEEHHHHCCHHHHHHHCCEECCCCCCCCCCCCCCCCHHEEECCCHHHHHHHHCCEEE
SNTYHLWLRPGEDIIREAGGLHKFMNWDGAILTDSGGFQVFSLSDMREIKEEGVHFRNHL
ECEEEEEECCCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHCCCHHHHCC
NGEKLFLSPEKAMDIQNALGSDIMMAFDECPPYPAEYDYMKASVERTSRWAERCLQAHNR
CCCEEEECCHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PHDQGLFGIVQGGEYEALRKQSAEDLVSLDFPGYAIGGLSVGEPKHVMNKVLEFTTPFLP
CCCCCCEEEEECCCHHHHHHCCHHHHEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCC
SNKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTCMTSNGRLVVRNAKYARDFN
CCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHCCCC
PIDEHCSCHVCKNYSRAYIRHLIKCNETFGFRLTTYHNLHFLLKLMEQVRTAIKEDRLGD
CHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCH
FKESFFEQYGLNKANPKNF
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNPITYELIKTDKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEDLQSMQANIIL
CCCEEEHHHHCCHHHHHHHCCEECCCCCCCCCCCCCCCCHHEEECCCHHHHHHHHCCEEE
SNTYHLWLRPGEDIIREAGGLHKFMNWDGAILTDSGGFQVFSLSDMREIKEEGVHFRNHL
ECEEEEEECCCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHCCCHHHHCC
NGEKLFLSPEKAMDIQNALGSDIMMAFDECPPYPAEYDYMKASVERTSRWAERCLQAHNR
CCCEEEECCHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PHDQGLFGIVQGGEYEALRKQSAEDLVSLDFPGYAIGGLSVGEPKHVMNKVLEFTTPFLP
CCCCCCEEEEECCCHHHHHHCCHHHHEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCC
SNKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTCMTSNGRLVVRNAKYARDFN
CCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHCCCC
PIDEHCSCHVCKNYSRAYIRHLIKCNETFGFRLTTYHNLHFLLKLMEQVRTAIKEDRLGD
CHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCH
FKESFFEQYGLNKANPKNF
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376