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The map label for this gene is miaA

Identifier: 23099089

GI number: 23099089

Start: 1675860

End: 1676801

Strand: Direct

Name: miaA

Synonym: OB1634

Alternate gene names: 23099089

Gene position: 1675860-1676801 (Clockwise)

Preceding gene: 23099088

Following gene: 23099090

Centisome position: 46.16

GC content: 35.46

Gene sequence:

>942_bases
ATGAAAGAAACTGTTATATCGATAGTCGGACCAACTGCAGTAGGAAAGTCACTACTAGGAATAGAAATGGCCAAGCGTTT
TAATGGAGAAGTCATTAGTGGTGACTCTACTCAAGTATATAAAGGCATGGATATTGGCACTGCAAAAGTTACAAAAGAGG
AAATGGATGGAATCATGCATCATATGATTGATATAATTTCTCCAGATGAATCCTTTTCAGTTGCTGATTTTCAATTACAC
GCGAAGAAATGTATTGATGACGTGTTAGGTAGAGGCAAACTTCCTATATTGGTTGGAGGTAGTGGGTTATATATACAAGC
TGTTTTATATAATTATAACTTTTCAGAGCAACGTCGTGATGAGTCATTCACGAAAAAACTAGAAGAGATTATTGAAGAAG
AAGGACCTAACCAATTATATACGAGACTAAAAGAAGTCGATCCTGTACAAGCTGCAAAAGTTCATCCGAATAATCATCGT
AGACTCATTCGTGCCCTTGAGGTTTATGAAACTACTGGAATGACTATGACAGAGTACCAACAGCAGCAACAGCTTGAATC
ACCCTATCACCTAATCTTAATTGGTTTAGATATGGATAGGGATGTATTATATGATCGAATAAATCAACGCGTGGATCATA
TGATTGATACTGGTTTAATAGAGGAAGTCAGGGATTTAATAGACCGAGGGTATGAAAACTGTCAATCTATGCATGCAATT
GGGTATAAAGAAATTATCCAGCATATTTCTGGTAATCAACCGATGGAATACGCAATCGATGCTTTAAAGCAGCATTCTAG
AAAATATGCAAAGCGACAGCTTACTTGGTTTCGAAATAAAATGAATGTTCAATGGTACAAGATGGAACCGTCAATAATCG
ATGAAAAAATTGAATTTATTTTGAATGATTTAGCAGGAATTTTAGAAGAAAAATCGAATTAA

Upstream 100 bases:

>100_bases
CCTACAGAAGATTTGTTGACAAGTCTACATGCTGATTTAGATAAAAAGAAATGGATGTTAGAATCCTATCAAAAGTGATG
GGACATTTAGGGGAATGTAT

Downstream 100 bases:

>100_bases
TTATTATAGATAGAAAAGAGGAGGAACTTTCCCATGGCTCAATCCGTTAATATTCAAGATCAGTACTTAAATCAGCTAAG
AAAGAATCATATTTCCGTTA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKETVISIVGPTAVGKSLLGIEMAKRFNGEVISGDSTQVYKGMDIGTAKVTKEEMDGIMHHMIDIISPDESFSVADFQLH
AKKCIDDVLGRGKLPILVGGSGLYIQAVLYNYNFSEQRRDESFTKKLEEIIEEEGPNQLYTRLKEVDPVQAAKVHPNNHR
RLIRALEVYETTGMTMTEYQQQQQLESPYHLILIGLDMDRDVLYDRINQRVDHMIDTGLIEEVRDLIDRGYENCQSMHAI
GYKEIIQHISGNQPMEYAIDALKQHSRKYAKRQLTWFRNKMNVQWYKMEPSIIDEKIEFILNDLAGILEEKSN

Sequences:

>Translated_313_residues
MKETVISIVGPTAVGKSLLGIEMAKRFNGEVISGDSTQVYKGMDIGTAKVTKEEMDGIMHHMIDIISPDESFSVADFQLH
AKKCIDDVLGRGKLPILVGGSGLYIQAVLYNYNFSEQRRDESFTKKLEEIIEEEGPNQLYTRLKEVDPVQAAKVHPNNHR
RLIRALEVYETTGMTMTEYQQQQQLESPYHLILIGLDMDRDVLYDRINQRVDHMIDTGLIEEVRDLIDRGYENCQSMHAI
GYKEIIQHISGNQPMEYAIDALKQHSRKYAKRQLTWFRNKMNVQWYKMEPSIIDEKIEFILNDLAGILEEKSN
>Mature_313_residues
MKETVISIVGPTAVGKSLLGIEMAKRFNGEVISGDSTQVYKGMDIGTAKVTKEEMDGIMHHMIDIISPDESFSVADFQLH
AKKCIDDVLGRGKLPILVGGSGLYIQAVLYNYNFSEQRRDESFTKKLEEIIEEEGPNQLYTRLKEVDPVQAAKVHPNNHR
RLIRALEVYETTGMTMTEYQQQQQLESPYHLILIGLDMDRDVLYDRINQRVDHMIDTGLIEEVRDLIDRGYENCQSMHAI
GYKEIIQHISGNQPMEYAIDALKQHSRKYAKRQLTWFRNKMNVQWYKMEPSIIDEKIEFILNDLAGILEEKSN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=315, Percent_Identity=33.968253968254, Blast_Score=181, Evalue=7e-46,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=35.9477124183007, Blast_Score=195, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI25144712, Length=302, Percent_Identity=32.1192052980132, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324848, Length=291, Percent_Identity=34.3642611683849, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24649830, Length=334, Percent_Identity=29.940119760479, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_OCEIH (Q8CXG5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692555.1
- ProteinModelPortal:   Q8CXG5
- SMR:   Q8CXG5
- GeneID:   1018129
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1634
- NMPDR:   fig|221109.1.peg.1636
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   OIHE221109:OB1634-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36029; Mature: 36029

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETVISIVGPTAVGKSLLGIEMAKRFNGEVISGDSTQVYKGMDIGTAKVTKEEMDGIMH
CCCCEEEEECCHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHH
HMIDIISPDESFSVADFQLHAKKCIDDVLGRGKLPILVGGSGLYIQAVLYNYNFSEQRRD
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCHHHHHH
ESFTKKLEEIIEEEGPNQLYTRLKEVDPVQAAKVHPNNHRRLIRALEVYETTGMTMTEYQ
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
QQQQLESPYHLILIGLDMDRDVLYDRINQRVDHMIDTGLIEEVRDLIDRGYENCQSMHAI
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYKEIIQHISGNQPMEYAIDALKQHSRKYAKRQLTWFRNKMNVQWYKMEPSIIDEKIEFI
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
LNDLAGILEEKSN
HHHHHHHHHCCCC
>Mature Secondary Structure
MKETVISIVGPTAVGKSLLGIEMAKRFNGEVISGDSTQVYKGMDIGTAKVTKEEMDGIMH
CCCCEEEEECCHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHH
HMIDIISPDESFSVADFQLHAKKCIDDVLGRGKLPILVGGSGLYIQAVLYNYNFSEQRRD
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCHHHHHH
ESFTKKLEEIIEEEGPNQLYTRLKEVDPVQAAKVHPNNHRRLIRALEVYETTGMTMTEYQ
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
QQQQLESPYHLILIGLDMDRDVLYDRINQRVDHMIDTGLIEEVRDLIDRGYENCQSMHAI
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYKEIIQHISGNQPMEYAIDALKQHSRKYAKRQLTWFRNKMNVQWYKMEPSIIDEKIEFI
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
LNDLAGILEEKSN
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376