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The map label for this gene is murD

Identifier: 23098924

GI number: 23098924

Start: 1508689

End: 1510038

Strand: Direct

Name: murD

Synonym: OB1469

Alternate gene names: 23098924

Gene position: 1508689-1510038 (Clockwise)

Preceding gene: 23098923

Following gene: 23098925

Centisome position: 41.56

GC content: 35.11

Gene sequence:

>1350_bases
ATGAATGTACTTACAAATTTTCCTTATCAGCACGTACTTGTATTAGGATTAGCTAAAAGTGGCACAGCAGCGGCAAACGT
TTTATTGCAAAATCATATACAAGTTACAATAAATGATGGCATGGCAACATTAGAAGACGCAACTGTACAGAAATTACAAA
CAATGGGAGCAGAATTAGTATTAGGCTCACATCCAATTTCTGTGCTTGATGGTAAAGATCTCATCGTAAAAAACCCAGGT
ATTCGCTATGATAATGTAATTGTAGAAGAAGCTCAGCGCCGAGGGATACCTGTTATATCAGAGGTTGAACTAGTTCATTA
CTTAACAAACCAGCCTGTTATTGGAATTACTGGATCAAATGGAAAAACGACAACAACAACATTAATTACAGAAATGCTAG
ATCGAAGTAATGTATCTGTAAAAGTGGCAGGGAATATTGGAGTAGTTGCAACAGAAGTAGCTTCTTCGTTGCAATCTGAT
GAAAAGATGGTTATGGAATTATCTTCTTTTCAGTTACAAGGAATAGATCAATTACAATTTTCAACTGCTGTCCTATTAAA
TTTATTCGAAGCACATTTAGATTATCATGGTTCTTTTGAAAATTACGTCGAAGCGAAATGTAATATATTTAAAAGTCAAA
ACAAACATGATTATTTAATTTATAATGCTGACGATGATAATGTATCTGCTGCAATTAAAACTGCAGAGGCTACAAAAGTG
CCGTTTTCTAGTTCACGACCGTTAGCAGATGGTGCATGGATGGATGATGATTTTCTATACTATAAAGATGAGAAAATTAT
AGCTATTCGAGACATAGTACTTGTAGGTAAACATAATATGGAAAACATATTAGCTGCTATTGCTACTGCAAAATTAAATG
GTGCTACTAATGAAGGGATTGTACAAGTGTTAACAACATTTTCAGGAGTAAAACATCGTTTGGAATTTGTAGGTGTTATA
AATGGGCGTTATATTTATAACGACTCAAAGGCAACGAATATATTAGCGACTAAAAAAGCGTTAGCAGCTTTTAACAAGAA
TGTGGTTTTACTAGCAGGTGGTTTAGATAGAGGAAATACATTTGAAGAATTAATTCCTTATTTGCATCATGTTAAAGCGA
TGGTCGTATTCGGAGAGACAGCAGGTAAGCTTAAGGATGCTGGTGTTGCTGCAAATATACCAGTAATTGAAAAAGCGTTA
GATGTTCAACATGCAGTAGAGGTTGCATTTGCTCTAACGGATGAGCAGGATACTATATTATTGTCACCAGCTTGTGCAAG
CTGGGATCAGTACAAAACTTTTGAAGAAAGAGGAGACATGTTTATACAAGCCCTGCATAGACTGAAGTAA

Upstream 100 bases:

>100_bases
ATGAACTATTAGGCTGGTCAGAGTGGCGTGTAGTCACTACTTTTTGGTTGGTAGGTTTATTGTTTGCTATGCTTGGTGTT
TATATTGAGGTGGGAATGTA

Downstream 100 bases:

>100_bases
GGGGCTTGTACACATGAAAATTCCAATTATTGTTACACCCCTTATTCTGAACTAAAGATTAGGGGTGTTTTTCTTTGTTG
AATAAACTAAAAGAGCAGCA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MNVLTNFPYQHVLVLGLAKSGTAAANVLLQNHIQVTINDGMATLEDATVQKLQTMGAELVLGSHPISVLDGKDLIVKNPG
IRYDNVIVEEAQRRGIPVISEVELVHYLTNQPVIGITGSNGKTTTTTLITEMLDRSNVSVKVAGNIGVVATEVASSLQSD
EKMVMELSSFQLQGIDQLQFSTAVLLNLFEAHLDYHGSFENYVEAKCNIFKSQNKHDYLIYNADDDNVSAAIKTAEATKV
PFSSSRPLADGAWMDDDFLYYKDEKIIAIRDIVLVGKHNMENILAAIATAKLNGATNEGIVQVLTTFSGVKHRLEFVGVI
NGRYIYNDSKATNILATKKALAAFNKNVVLLAGGLDRGNTFEELIPYLHHVKAMVVFGETAGKLKDAGVAANIPVIEKAL
DVQHAVEVAFALTDEQDTILLSPACASWDQYKTFEERGDMFIQALHRLK

Sequences:

>Translated_449_residues
MNVLTNFPYQHVLVLGLAKSGTAAANVLLQNHIQVTINDGMATLEDATVQKLQTMGAELVLGSHPISVLDGKDLIVKNPG
IRYDNVIVEEAQRRGIPVISEVELVHYLTNQPVIGITGSNGKTTTTTLITEMLDRSNVSVKVAGNIGVVATEVASSLQSD
EKMVMELSSFQLQGIDQLQFSTAVLLNLFEAHLDYHGSFENYVEAKCNIFKSQNKHDYLIYNADDDNVSAAIKTAEATKV
PFSSSRPLADGAWMDDDFLYYKDEKIIAIRDIVLVGKHNMENILAAIATAKLNGATNEGIVQVLTTFSGVKHRLEFVGVI
NGRYIYNDSKATNILATKKALAAFNKNVVLLAGGLDRGNTFEELIPYLHHVKAMVVFGETAGKLKDAGVAANIPVIEKAL
DVQHAVEVAFALTDEQDTILLSPACASWDQYKTFEERGDMFIQALHRLK
>Mature_449_residues
MNVLTNFPYQHVLVLGLAKSGTAAANVLLQNHIQVTINDGMATLEDATVQKLQTMGAELVLGSHPISVLDGKDLIVKNPG
IRYDNVIVEEAQRRGIPVISEVELVHYLTNQPVIGITGSNGKTTTTTLITEMLDRSNVSVKVAGNIGVVATEVASSLQSD
EKMVMELSSFQLQGIDQLQFSTAVLLNLFEAHLDYHGSFENYVEAKCNIFKSQNKHDYLIYNADDDNVSAAIKTAEATKV
PFSSSRPLADGAWMDDDFLYYKDEKIIAIRDIVLVGKHNMENILAAIATAKLNGATNEGIVQVLTTFSGVKHRLEFVGVI
NGRYIYNDSKATNILATKKALAAFNKNVVLLAGGLDRGNTFEELIPYLHHVKAMVVFGETAGKLKDAGVAANIPVIEKAL
DVQHAVEVAFALTDEQDTILLSPACASWDQYKTFEERGDMFIQALHRLK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=454, Percent_Identity=31.7180616740088, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_OCEIH (Q8ER50)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692390.1
- ProteinModelPortal:   Q8ER50
- SMR:   Q8ER50
- GeneID:   1017716
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1469
- NMPDR:   fig|221109.1.peg.1471
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02472
- BioCyc:   OIHE221109:OB1469-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49048; Mature: 49048

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLTNFPYQHVLVLGLAKSGTAAANVLLQNHIQVTINDGMATLEDATVQKLQTMGAELV
CCCCCCCCHHEEEEEEECCCCCHHHHHEEECCEEEEECCCCCHHHHHHHHHHHHCCCEEE
LGSHPISVLDGKDLIVKNPGIRYDNVIVEEAQRRGIPVISEVELVHYLTNQPVIGITGSN
ECCCCEEEECCCEEEEECCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHCCCCEEEEECCC
GKTTTTTLITEMLDRSNVSVKVAGNIGVVATEVASSLQSDEKMVMELSSFQLQGIDQLQF
CCCHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHH
STAVLLNLFEAHLDYHGSFENYVEAKCNIFKSQNKHDYLIYNADDDNVSAAIKTAEATKV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECHHCCC
PFSSSRPLADGAWMDDDFLYYKDEKIIAIRDIVLVGKHNMENILAAIATAKLNGATNEGI
CCCCCCCCCCCCCCCCCEEEEECCEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHH
VQVLTTFSGVKHRLEFVGVINGRYIYNDSKATNILATKKALAAFNKNVVLLAGGLDRGNT
HHHHHHHHHHHHEEEEEEEECCEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCC
FEELIPYLHHVKAMVVFGETAGKLKDAGVAANIPVIEKALDVQHAVEVAFALTDEQDTIL
HHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEE
LSPACASWDQYKTFEERGDMFIQALHRLK
EECCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNVLTNFPYQHVLVLGLAKSGTAAANVLLQNHIQVTINDGMATLEDATVQKLQTMGAELV
CCCCCCCCHHEEEEEEECCCCCHHHHHEEECCEEEEECCCCCHHHHHHHHHHHHCCCEEE
LGSHPISVLDGKDLIVKNPGIRYDNVIVEEAQRRGIPVISEVELVHYLTNQPVIGITGSN
ECCCCEEEECCCEEEEECCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHCCCCEEEEECCC
GKTTTTTLITEMLDRSNVSVKVAGNIGVVATEVASSLQSDEKMVMELSSFQLQGIDQLQF
CCCHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHH
STAVLLNLFEAHLDYHGSFENYVEAKCNIFKSQNKHDYLIYNADDDNVSAAIKTAEATKV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECHHCCC
PFSSSRPLADGAWMDDDFLYYKDEKIIAIRDIVLVGKHNMENILAAIATAKLNGATNEGI
CCCCCCCCCCCCCCCCCEEEEECCEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHH
VQVLTTFSGVKHRLEFVGVINGRYIYNDSKATNILATKKALAAFNKNVVLLAGGLDRGNT
HHHHHHHHHHHHEEEEEEEECCEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCC
FEELIPYLHHVKAMVVFGETAGKLKDAGVAANIPVIEKALDVQHAVEVAFALTDEQDTIL
HHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEE
LSPACASWDQYKTFEERGDMFIQALHRLK
EECCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376