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The map label for this gene is opuD [H]

Identifier: 23098713

GI number: 23098713

Start: 1302791

End: 1304281

Strand: Direct

Name: opuD [H]

Synonym: OB1258

Alternate gene names: 23098713

Gene position: 1302791-1304281 (Clockwise)

Preceding gene: 23098712

Following gene: 23098717

Centisome position: 35.88

GC content: 36.22

Gene sequence:

>1491_bases
ATGAAAAGTGTTACGAGTGTTTTTTGGTATTCATTAGCATTATGTATTATCGTGGTCCTGTGGGGGTCCTTTGCCCCAAG
TAATTTAGAAGGATTTACGTCAAATGTCACAACATTTATATCGGATACCTTTGGTTGGTATTATTTAATCATAGTTATGT
TAATGTTGATTTTTTGTGTTTATCTTATTTTTTCTAAATATGGAAAAATTAAATTAGGTAAAGAGAATGATAAACCCGAA
TTTAGTTTATTGTCTTGGTTTGCTATGTTATTTAGTGCTGGAATGGGGATGGGTCTAGTATTTTGGACTACTGCAGAACC
TATTTCACATGCATTTACTAGTTCTCCTGGTGCAGAACTAGGATCAGAACAAGCAATTAACGATGGATTAAAGTATTCTT
TTTTTCATTGGGGAGTACATGCGTGGGCAGTTTATGGAATTGTAGCATTAGTTTTAGCGTATTTTAAGTTTTATAAAGAC
ACACCGGGGTTAATTAGTGCGACTTTGGTTCCGTTATTTGGAAGAAATAGTATGCAAGGTCCAGCTGGGAAAATTATTGA
TACGCTAGCAGTCTTTGCTACAGTTGTCGGTGTTGCAGCAACTCTTGGGTTTGGATCTGCACAGATTAGTGGGGGACTTT
CTTATTTATTTGGTACTCCAGGGACCTTTTGGATGCAATTACTGATACTCGTGATTGCTACCATTCTATTTATTTGTTCT
GCCTGGTCAGGAATCGGTCGGGGGATTAAGTATTTAAGTAATATTAATATGGGATTAGCGTTTATACTGTTGCTTATGTT
ATTTCTAGTTGGTCCTACGCTATATATTCTTAATATGTTTACACATACATTGGGAAGTTATGTATCTGATTTCTTTGATA
TGAGCTTAAGATTGGCACCTCAAGATGGTGAACAAAGAACATGGATTAATAATTGGACAGTATTTTATTGGGCATGGTGG
ATATCTTGGTCACCGTTTGTCGGAATTTTTATTGCACGTATTTCCAAAGGAAGAACAATTAAAGAATTTATGCTTGGGGT
GTTATTAGTACCGGCGATTGTATGTTTTATTTTCTTTGCGGTATTCGGAGTCTCTGCACTAAACTTAGAACAGAATGGCA
TTGCTACAATATCAGAATTTGCATTAGAAACATCAACGTTTGGTGTGTTAAATGAATATCCACTAGGTATGATAATGTCT
ATCATCACTTTATTTGTTGTAGCGATATTCTTTATTACATCTGCAGATTCAGCGACATTCGTTCTTGGTATGTTAAGTAC
TGGAGGAACAATTAATCCGAAAAACGCTGTTAAAATTATGTGGGGACTTATGCAATCTGCAGTGGCTGCCATCATTGTAT
ATTTCGGGGGGACGCAAGGTTTGCAGAATATGTTAATTATCGCTGCTCTCCCGTTCTCTATAGTAATTATTCTTATGGGT
GTTTCGTTCTTTAAATCTGCCCGTAAGGACCAACGGCATTGGAGAAAGTAA

Upstream 100 bases:

>100_bases
TTGGAACATTTTAACAAATTTTGGTTTGATCGTATAACGGGTCGGGTATTAATTAAGATGCTTATACATAAAAGAAAAGC
AATAAAGAAGAGGTGTATGG

Downstream 100 bases:

>100_bases
AAAAATAAGTATAGTGATCATCTTTAATGAAGATCACTATACTTATTTTATTTGTCTATTATTTAATTCATAAAAATCTT
CCCTGGATTAAGAATACCAG

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MKSVTSVFWYSLALCIIVVLWGSFAPSNLEGFTSNVTTFISDTFGWYYLIIVMLMLIFCVYLIFSKYGKIKLGKENDKPE
FSLLSWFAMLFSAGMGMGLVFWTTAEPISHAFTSSPGAELGSEQAINDGLKYSFFHWGVHAWAVYGIVALVLAYFKFYKD
TPGLISATLVPLFGRNSMQGPAGKIIDTLAVFATVVGVAATLGFGSAQISGGLSYLFGTPGTFWMQLLILVIATILFICS
AWSGIGRGIKYLSNINMGLAFILLLMLFLVGPTLYILNMFTHTLGSYVSDFFDMSLRLAPQDGEQRTWINNWTVFYWAWW
ISWSPFVGIFIARISKGRTIKEFMLGVLLVPAIVCFIFFAVFGVSALNLEQNGIATISEFALETSTFGVLNEYPLGMIMS
IITLFVVAIFFITSADSATFVLGMLSTGGTINPKNAVKIMWGLMQSAVAAIIVYFGGTQGLQNMLIIAALPFSIVIILMG
VSFFKSARKDQRHWRK

Sequences:

>Translated_496_residues
MKSVTSVFWYSLALCIIVVLWGSFAPSNLEGFTSNVTTFISDTFGWYYLIIVMLMLIFCVYLIFSKYGKIKLGKENDKPE
FSLLSWFAMLFSAGMGMGLVFWTTAEPISHAFTSSPGAELGSEQAINDGLKYSFFHWGVHAWAVYGIVALVLAYFKFYKD
TPGLISATLVPLFGRNSMQGPAGKIIDTLAVFATVVGVAATLGFGSAQISGGLSYLFGTPGTFWMQLLILVIATILFICS
AWSGIGRGIKYLSNINMGLAFILLLMLFLVGPTLYILNMFTHTLGSYVSDFFDMSLRLAPQDGEQRTWINNWTVFYWAWW
ISWSPFVGIFIARISKGRTIKEFMLGVLLVPAIVCFIFFAVFGVSALNLEQNGIATISEFALETSTFGVLNEYPLGMIMS
IITLFVVAIFFITSADSATFVLGMLSTGGTINPKNAVKIMWGLMQSAVAAIIVYFGGTQGLQNMLIIAALPFSIVIILMG
VSFFKSARKDQRHWRK
>Mature_496_residues
MKSVTSVFWYSLALCIIVVLWGSFAPSNLEGFTSNVTTFISDTFGWYYLIIVMLMLIFCVYLIFSKYGKIKLGKENDKPE
FSLLSWFAMLFSAGMGMGLVFWTTAEPISHAFTSSPGAELGSEQAINDGLKYSFFHWGVHAWAVYGIVALVLAYFKFYKD
TPGLISATLVPLFGRNSMQGPAGKIIDTLAVFATVVGVAATLGFGSAQISGGLSYLFGTPGTFWMQLLILVIATILFICS
AWSGIGRGIKYLSNINMGLAFILLLMLFLVGPTLYILNMFTHTLGSYVSDFFDMSLRLAPQDGEQRTWINNWTVFYWAWW
ISWSPFVGIFIARISKGRTIKEFMLGVLLVPAIVCFIFFAVFGVSALNLEQNGIATISEFALETSTFGVLNEYPLGMIMS
IITLFVVAIFFITSADSATFVLGMLSTGGTINPKNAVKIMWGLMQSAVAAIIVYFGGTQGLQNMLIIAALPFSIVIILMG
VSFFKSARKDQRHWRK

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=461, Percent_Identity=37.0932754880694, Blast_Score=308, Evalue=8e-85,
Organism=Escherichia coli, GI1788102, Length=451, Percent_Identity=31.9290465631929, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1786224, Length=468, Percent_Identity=29.0598290598291, Blast_Score=195, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 54754; Mature: 54754

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]