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The map label for this gene is ligA

Identifier: 23098215

GI number: 23098215

Start: 810517

End: 812520

Strand: Direct

Name: ligA

Synonym: OB0760

Alternate gene names: 23098215

Gene position: 810517-812520 (Clockwise)

Preceding gene: 23098214

Following gene: 23098216

Centisome position: 22.33

GC content: 38.07

Gene sequence:

>2004_bases
ATGAATAAAGAACAAGTAAGTAAGAAGATAGAATCCTTAATTGAATTGTTAAATCAATATGGATATGAATATTATGTGTT
GGATAATCCTACAGTTCCTGATGCGGAATACGATCAGAAATTAAGGGAGTTGCAACAATTGGAGCAGGACTTTCCAGACT
TAGTAATGGAAAATTCACCTACCCAGCGTGTAGGTGGAGCTCCGTTAGCATCATTTCAAAAAGTAACCCATAATGTTCCT
ATGCTAAGTCTTGGAAATGCATTTAATGAACAAGATTTACGTGATTTCGCAAGACGTGCTAGTAACGGAACAGATCAACC
AATCAGCTTTGTATGCGAGCTTAAAATCGATGGTCTTGCTATTTCTCTTACATATGAAAATGGTAAGTTTGTCCGAGGAG
CTACTCGTGGAGATGGAACAATTGGAGAAGATATTACCTCTAATTTAAAAACAATCCGCAGTATCCCTTTATCGATAAAA
GAACAAGGAACATTAGAAGTCCGCGGAGAAGCATATATGCCACATAAGTCATTTCTTGCATTAAATGAACTACGTGAGAA
AAATGAAGAGGAACCTTTTGCGAATCCGAGAAATGCTGCTGCTGGATCATTGCGTCAACTCGATCCAAAGATTGCGGCAA
AGCGGAATTTAGATATTTTTCTATACGGTGTAGGAGAATGGGAAAATAGCGACCTTACATCGCATAGTGAGCATTTAACT
CGTTTAAAAGAATTAGGATTTAAGACAAACAAGGAATGGAAAAAATGTAATACGATAGATGAGGTTATTGAATACGTAAA
CTATTGGACAGAACATCGAAATGACCTGTCCTATGAAATTGATGGAATAGTAATCAAAGTAGATAGTCTCGACCAGCAGG
AAGAATTAGGATTTACAGCGAAAAGCCCCCGTTGGGCAATTGCTTATAAATTTCCTGCAGAAGAAGCGATGACGACCCTA
AGAGATATTGAATTGAGTATTGGAAGAACGGGTGTTGTAACACCAACAGCAATATTGGATCCAGTGCGAGTAGCAGGTAC
GACAGTTCAGCGTGCTTCTCTACATAATGAAGATTTAATTCGCGAACAAGATATCCGTATTGGTGATAAAGTAGTGATAA
AAAAAGCAGGAGATATTATTCCAAAAGTAGTCCGTTCTGTAGTCGAACAACGAAACGGGGATGAACAAGAATTCCATATG
CCGGATGAGTGTCCTGCATGTGGCAATGAACTTGTCAGATTGGAAGAAGAAGTCGCTCTTCGGTGTATCAATCCGAATTG
CCCTGCTCAATTAATGGAGGGATTAATCCATTTCGTTTCTCGTAATGCTATGAATATTGATGGGCTTGGCGAAAAAGTAA
TTATTCAGTTGTTCCAAGAGAATCTTGTTCAAACTATGGCTGATCTTTATCGACTGGATAAGGAAGAACTCTTACAATTA
GAAAGAATGGGAGAAAAATCAGTTACAAATCTTTTAGAAGCAATTGAGAAATCGAAAGAAAATTCATTGGAACGTCTTTT
ATTCGGATTAGGAATTCGATTTATAGGTTCAAAAGCAGCGAAAACACTGGCGATGGAGTTTGAGACAATGGAAAATCTTC
AAAAAGCAACCTATGAAGATGTGTTAGCGATAGATGAGATTGGAGATAAGATGGCTGATTCCATTGTACAATATTTTGCT
GAAGAAAAAGTTACAGAACTCTTAAGCAACTTACGGGAGCTAGGAGTTCAAATGGAATATAAAGGTCCGCGTAAATCGGA
ACAGGCAACAGATAGTGTATTGTCAGGAAAAACAGTGGTACTTACAGGCAAGATGGAGAAGTTTTCTAGAAAAGAGGCAA
AAGAAATAATTGAATCATTGGGTGGCACTGTAACAGGAAGTGTCAGCAAGAAAACGGATATTGTTATTGCTGGTGAGGAT
GCTGGTTCAAAATTGGATAAAGCAGAGAAATTAGGAATTGACGTGTGGAGTGAACAACAGCTAGAAGATGTTGTTGGGAA
GTAA

Upstream 100 bases:

>100_bases
AAAAAATGGAATTAGATATCGCTTTTCCTGCACCTATAGGTATTAAACGGTTATTAGCTAAATTTGCACCAATTACGAAG
GAAGAATAGGGGCAACGAAA

Downstream 100 bases:

>100_bases
GGAGGGAGCAGCTTGAAAAAAGTCAGCTTGTTAATGTTAGTGCTACTTCTTTTGTTAAGTGGATGTCTTCAAAATCAAAA
TAGTGAAGAAGAAGTAGTGC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MNKEQVSKKIESLIELLNQYGYEYYVLDNPTVPDAEYDQKLRELQQLEQDFPDLVMENSPTQRVGGAPLASFQKVTHNVP
MLSLGNAFNEQDLRDFARRASNGTDQPISFVCELKIDGLAISLTYENGKFVRGATRGDGTIGEDITSNLKTIRSIPLSIK
EQGTLEVRGEAYMPHKSFLALNELREKNEEEPFANPRNAAAGSLRQLDPKIAAKRNLDIFLYGVGEWENSDLTSHSEHLT
RLKELGFKTNKEWKKCNTIDEVIEYVNYWTEHRNDLSYEIDGIVIKVDSLDQQEELGFTAKSPRWAIAYKFPAEEAMTTL
RDIELSIGRTGVVTPTAILDPVRVAGTTVQRASLHNEDLIREQDIRIGDKVVIKKAGDIIPKVVRSVVEQRNGDEQEFHM
PDECPACGNELVRLEEEVALRCINPNCPAQLMEGLIHFVSRNAMNIDGLGEKVIIQLFQENLVQTMADLYRLDKEELLQL
ERMGEKSVTNLLEAIEKSKENSLERLLFGLGIRFIGSKAAKTLAMEFETMENLQKATYEDVLAIDEIGDKMADSIVQYFA
EEKVTELLSNLRELGVQMEYKGPRKSEQATDSVLSGKTVVLTGKMEKFSRKEAKEIIESLGGTVTGSVSKKTDIVIAGED
AGSKLDKAEKLGIDVWSEQQLEDVVGK

Sequences:

>Translated_667_residues
MNKEQVSKKIESLIELLNQYGYEYYVLDNPTVPDAEYDQKLRELQQLEQDFPDLVMENSPTQRVGGAPLASFQKVTHNVP
MLSLGNAFNEQDLRDFARRASNGTDQPISFVCELKIDGLAISLTYENGKFVRGATRGDGTIGEDITSNLKTIRSIPLSIK
EQGTLEVRGEAYMPHKSFLALNELREKNEEEPFANPRNAAAGSLRQLDPKIAAKRNLDIFLYGVGEWENSDLTSHSEHLT
RLKELGFKTNKEWKKCNTIDEVIEYVNYWTEHRNDLSYEIDGIVIKVDSLDQQEELGFTAKSPRWAIAYKFPAEEAMTTL
RDIELSIGRTGVVTPTAILDPVRVAGTTVQRASLHNEDLIREQDIRIGDKVVIKKAGDIIPKVVRSVVEQRNGDEQEFHM
PDECPACGNELVRLEEEVALRCINPNCPAQLMEGLIHFVSRNAMNIDGLGEKVIIQLFQENLVQTMADLYRLDKEELLQL
ERMGEKSVTNLLEAIEKSKENSLERLLFGLGIRFIGSKAAKTLAMEFETMENLQKATYEDVLAIDEIGDKMADSIVQYFA
EEKVTELLSNLRELGVQMEYKGPRKSEQATDSVLSGKTVVLTGKMEKFSRKEAKEIIESLGGTVTGSVSKKTDIVIAGED
AGSKLDKAEKLGIDVWSEQQLEDVVGK
>Mature_667_residues
MNKEQVSKKIESLIELLNQYGYEYYVLDNPTVPDAEYDQKLRELQQLEQDFPDLVMENSPTQRVGGAPLASFQKVTHNVP
MLSLGNAFNEQDLRDFARRASNGTDQPISFVCELKIDGLAISLTYENGKFVRGATRGDGTIGEDITSNLKTIRSIPLSIK
EQGTLEVRGEAYMPHKSFLALNELREKNEEEPFANPRNAAAGSLRQLDPKIAAKRNLDIFLYGVGEWENSDLTSHSEHLT
RLKELGFKTNKEWKKCNTIDEVIEYVNYWTEHRNDLSYEIDGIVIKVDSLDQQEELGFTAKSPRWAIAYKFPAEEAMTTL
RDIELSIGRTGVVTPTAILDPVRVAGTTVQRASLHNEDLIREQDIRIGDKVVIKKAGDIIPKVVRSVVEQRNGDEQEFHM
PDECPACGNELVRLEEEVALRCINPNCPAQLMEGLIHFVSRNAMNIDGLGEKVIIQLFQENLVQTMADLYRLDKEELLQL
ERMGEKSVTNLLEAIEKSKENSLERLLFGLGIRFIGSKAAKTLAMEFETMENLQKATYEDVLAIDEIGDKMADSIVQYFA
EEKVTELLSNLRELGVQMEYKGPRKSEQATDSVLSGKTVVLTGKMEKFSRKEAKEIIESLGGTVTGSVSKKTDIVIAGED
AGSKLDKAEKLGIDVWSEQQLEDVVGK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=670, Percent_Identity=47.3134328358209, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI87082305, Length=508, Percent_Identity=22.8346456692913, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_OCEIH (Q8CXK6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691681.1
- HSSP:   O87703
- ProteinModelPortal:   Q8CXK6
- SMR:   Q8CXK6
- GeneID:   1016286
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0760
- NMPDR:   fig|221109.1.peg.768
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   OIHE221109:OB0760-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74919; Mature: 74919

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 170-170 BINDING 286-286 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEQVSKKIESLIELLNQYGYEYYVLDNPTVPDAEYDQKLRELQQLEQDFPDLVMENSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCC
TQRVGGAPLASFQKVTHNVPMLSLGNAFNEQDLRDFARRASNGTDQPISFVCELKIDGLA
CCCCCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCEE
ISLTYENGKFVRGATRGDGTIGEDITSNLKTIRSIPLSIKEQGTLEVRGEAYMPHKSFLA
EEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCEECCHHHHHH
LNELREKNEEEPFANPRNAAAGSLRQLDPKIAAKRNLDIFLYGVGEWENSDLTSHSEHLT
HHHHHHCCCCCCCCCCCCHHHCHHHHCCHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHH
RLKELGFKTNKEWKKCNTIDEVIEYVNYWTEHRNDLSYEIDGIVIKVDSLDQQEELGFTA
HHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCEEECEEEEEECCCCCHHHCCCCC
KSPRWAIAYKFPAEEAMTTLRDIELSIGRTGVVTPTAILDPVRVAGTTVQRASLHNEDLI
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCHHHHHHCCCHHHH
REQDIRIGDKVVIKKAGDIIPKVVRSVVEQRNGDEQEFHMPDECPACGNELVRLEEEVAL
HHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RCINPNCPAQLMEGLIHFVSRNAMNIDGLGEKVIIQLFQENLVQTMADLYRLDKEELLQL
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
ERMGEKSVTNLLEAIEKSKENSLERLLFGLGIRFIGSKAAKTLAMEFETMENLQKATYED
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAIDEIGDKMADSIVQYFAEEKVTELLSNLRELGVQMEYKGPRKSEQATDSVLSGKTVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCCEEE
LTGKMEKFSRKEAKEIIESLGGTVTGSVSKKTDIVIAGEDAGSKLDKAEKLGIDVWSEQQ
EECCHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCHHH
LEDVVGK
HHHHHCC
>Mature Secondary Structure
MNKEQVSKKIESLIELLNQYGYEYYVLDNPTVPDAEYDQKLRELQQLEQDFPDLVMENSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCC
TQRVGGAPLASFQKVTHNVPMLSLGNAFNEQDLRDFARRASNGTDQPISFVCELKIDGLA
CCCCCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCEE
ISLTYENGKFVRGATRGDGTIGEDITSNLKTIRSIPLSIKEQGTLEVRGEAYMPHKSFLA
EEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCEECCHHHHHH
LNELREKNEEEPFANPRNAAAGSLRQLDPKIAAKRNLDIFLYGVGEWENSDLTSHSEHLT
HHHHHHCCCCCCCCCCCCHHHCHHHHCCHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHH
RLKELGFKTNKEWKKCNTIDEVIEYVNYWTEHRNDLSYEIDGIVIKVDSLDQQEELGFTA
HHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCEEECEEEEEECCCCCHHHCCCCC
KSPRWAIAYKFPAEEAMTTLRDIELSIGRTGVVTPTAILDPVRVAGTTVQRASLHNEDLI
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCHHHHHHCCCHHHH
REQDIRIGDKVVIKKAGDIIPKVVRSVVEQRNGDEQEFHMPDECPACGNELVRLEEEVAL
HHHCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RCINPNCPAQLMEGLIHFVSRNAMNIDGLGEKVIIQLFQENLVQTMADLYRLDKEELLQL
EECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
ERMGEKSVTNLLEAIEKSKENSLERLLFGLGIRFIGSKAAKTLAMEFETMENLQKATYED
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAIDEIGDKMADSIVQYFAEEKVTELLSNLRELGVQMEYKGPRKSEQATDSVLSGKTVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCCEEE
LTGKMEKFSRKEAKEIIESLGGTVTGSVSKKTDIVIAGEDAGSKLDKAEKLGIDVWSEQQ
EECCHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCHHH
LEDVVGK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376