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The map label for this gene is adaB [H]

Identifier: 23098191

GI number: 23098191

Start: 783958

End: 784461

Strand: Direct

Name: adaB [H]

Synonym: OB0736

Alternate gene names: 23098191

Gene position: 783958-784461 (Clockwise)

Preceding gene: 23098190

Following gene: 23098192

Centisome position: 21.59

GC content: 35.91

Gene sequence:

>504_bases
ATGAACAACGTGCTATATTATTCAAAAATTACGTTTGATAATGGAAGTATATGGGTTTTAGGAACGGATAAAGATATCTA
TTATATAGGAGGATATAATTCAGGATTTAATGATTTGTCATTATGGGCTAAGAAAAAATATCCTGCTTATGAACTGATTG
GTGACGATCAAATTTTAGAAACCGCAGTAGAGGAAATGAAGAGTTATTTTGATGGAAAACTTACAGACTTTTCTTTTGAA
GTCGATTATTCTTATGGCACCCCTTTTCAACAGAAGGTTTGGCATGCTTTACAAGAAGTTCCTTATGGTGAAACGGTTAC
GTATACTGACATTGCTAATCAAATAGGAAGACCCGATGCAGTGAGAGCAGTAGCTGGTGCAATTGGTGCAAATCAACTAT
TGATAGCCATTCCCTGCCATCGTGTTATTGGGAAGAACGGAAAGTTAGTCGGTTATCGAGGCGGCATCGAAATGAAAGAA
TCATTATTAAAATTAGAGTCATAG

Upstream 100 bases:

>100_bases
GGGCTGCAGATGATTTGTTTCAATGTGAAGCTTTAGGCCATACGTGGAGAAAAGCTTTGAGGAAGCGTTTAAGAGGCTAT
TGAATAGGAGGGAACGTGTC

Downstream 100 bases:

>100_bases
GCGGTGGTCTATGACTCTATTCTTTTGATAAGAAAGTAAAAAGATTTGGTACTACAACTAACCGTAAACAGAACGGCTAC
GGACAACTTCAACTCACCCA

Product: methylated-DNA-protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MNNVLYYSKITFDNGSIWVLGTDKDIYYIGGYNSGFNDLSLWAKKKYPAYELIGDDQILETAVEEMKSYFDGKLTDFSFE
VDYSYGTPFQQKVWHALQEVPYGETVTYTDIANQIGRPDAVRAVAGAIGANQLLIAIPCHRVIGKNGKLVGYRGGIEMKE
SLLKLES

Sequences:

>Translated_167_residues
MNNVLYYSKITFDNGSIWVLGTDKDIYYIGGYNSGFNDLSLWAKKKYPAYELIGDDQILETAVEEMKSYFDGKLTDFSFE
VDYSYGTPFQQKVWHALQEVPYGETVTYTDIANQIGRPDAVRAVAGAIGANQLLIAIPCHRVIGKNGKLVGYRGGIEMKE
SLLKLES
>Mature_167_residues
MNNVLYYSKITFDNGSIWVLGTDKDIYYIGGYNSGFNDLSLWAKKKYPAYELIGDDQILETAVEEMKSYFDGKLTDFSFE
VDYSYGTPFQQKVWHALQEVPYGETVTYTDIANQIGRPDAVRAVAGAIGANQLLIAIPCHRVIGKNGKLVGYRGGIEMKE
SLLKLES

Specific function: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=177, Percent_Identity=29.3785310734463, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1788542, Length=124, Percent_Identity=43.5483870967742, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=44.6601941747573, Blast_Score=105, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=53.75, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=53.75, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=48.2352941176471, Blast_Score=96, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137554, Length=120, Percent_Identity=39.1666666666667, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18729; Mature: 18729

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVLYYSKITFDNGSIWVLGTDKDIYYIGGYNSGFNDLSLWAKKKYPAYELIGDDQILE
CCCEEEEEEEEECCCEEEEEECCCCEEEECCCCCCCCCHHHEECCCCCCEEECCCHHHHH
TAVEEMKSYFDGKLTDFSFEVDYSYGTPFQQKVWHALQEVPYGETVTYTDIANQIGRPDA
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCHH
VRAVAGAIGANQLLIAIPCHRVIGKNGKLVGYRGGIEMKESLLKLES
HHHHHHHCCCCEEEEEECCHHHHCCCCCEEEEECCCHHHHHHHHCCC
>Mature Secondary Structure
MNNVLYYSKITFDNGSIWVLGTDKDIYYIGGYNSGFNDLSLWAKKKYPAYELIGDDQILE
CCCEEEEEEEEECCCEEEEEECCCCEEEECCCCCCCCCHHHEECCCCCCEEECCCHHHHH
TAVEEMKSYFDGKLTDFSFEVDYSYGTPFQQKVWHALQEVPYGETVTYTDIANQIGRPDA
HHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCHH
VRAVAGAIGANQLLIAIPCHRVIGKNGKLVGYRGGIEMKESLLKLES
HHHHHHHCCCCEEEEEECCHHHHCCCCCEEEEECCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]