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The map label for this gene is argF

Identifier: 23097915

GI number: 23097915

Start: 485838

End: 486806

Strand: Direct

Name: argF

Synonym: OB0460

Alternate gene names: 23097915

Gene position: 485838-486806 (Clockwise)

Preceding gene: 23097914

Following gene: 23097917

Centisome position: 13.38

GC content: 36.64

Gene sequence:

>969_bases
ATGACAACGAATACTCAAGAAATTAATTTTCAACAACAAGAGTTAAAGGGAAGAGACTTCTTAACTTTAGCGGATTATAC
AAAAGATGAGATTAATTACTTAATTGATTTAGCAGACTACTTGAAAAAACAACATAAAGATGGAGTTATTTTTGAGCCTT
TAAAAGGAAAAACGTTAGGAATGATCTTTGAGAAGTCATCTACGAGAACGAGAGTATCTTTTGAAACGGGAATCTACCAA
CTTGGAGGGTTAGGGATTTTTCTCAGTTCCAAAGATATTCAGATTGGTAGAGGAGAGACGATTGCAGATACGGCGAAGGT
TCTTTCTGGATATTTAGATGGTATTATGATTCGAACGTTCGCACAAGAAGATGTGGAGGAATTAGCAAAGCATGCTAGTA
TTCCTGTTATTAATGGATTAACGGATTTGTATCATCCTTGTCAGGTATTGGCTGATTTACAAACGATTAAGGAGGTAAAA
GGTAAACTCCATGGAGTAAAATTGGTCTACATTGGTGATGGTAATAACATGACTCATTCTTTAATGTTAGGTGCCGCAAA
AACAGGCATGCATATGGTAGCCGTAACACCTGATGGATACACACCTAATAAAGAAGTATTAACTAAAGCGAAAAGTATAG
CTAATCAACATGGGGCTATTATCGAATGGACATCTGATATAGCAACGGCAGTACAAGAGGCAGATGTTATTTATACAGAT
GTATGGGCAAGTATGGGACAGGAAGAAGAGCAACAACAACGAGAAAAAGACTTTGAGAATTATCAAGTAAATGAAAGTCT
GTTAGAAAAAGCGAAAACGGATGTGATTTTTATGCATTGCTTACCAGCTCATCGAGGAGAAGAAGTGACTGCAGGAGTAA
TTGATGGAAAACAATCTGTCGTATTTCAGCAAGCAGAAAATCGTCTACATGCACAAAAAGCATTAATGACAGCGTTAATG
GCAGATTGA

Upstream 100 bases:

>100_bases
TAATTTAAATTTAATAAATAAACAGTTGAAAACTATTGTATAATTATTCTTTTTGTTGCATAATAATTCATACAACCATT
TTGGAAAGGTGTGCGTGAAG

Downstream 100 bases:

>100_bases
TAAAGAGAAGAGTTAGATTTAGAGGCAATATTTAAGGAGATAAAAATTCGAACGTTGGATTCTGGATTGGTCGGAATTTT
AATGAACTTAGTATTGTAAT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTTNTQEINFQQQELKGRDFLTLADYTKDEINYLIDLADYLKKQHKDGVIFEPLKGKTLGMIFEKSSTRTRVSFETGIYQ
LGGLGIFLSSKDIQIGRGETIADTAKVLSGYLDGIMIRTFAQEDVEELAKHASIPVINGLTDLYHPCQVLADLQTIKEVK
GKLHGVKLVYIGDGNNMTHSLMLGAAKTGMHMVAVTPDGYTPNKEVLTKAKSIANQHGAIIEWTSDIATAVQEADVIYTD
VWASMGQEEEQQQREKDFENYQVNESLLEKAKTDVIFMHCLPAHRGEEVTAGVIDGKQSVVFQQAENRLHAQKALMTALM
AD

Sequences:

>Translated_322_residues
MTTNTQEINFQQQELKGRDFLTLADYTKDEINYLIDLADYLKKQHKDGVIFEPLKGKTLGMIFEKSSTRTRVSFETGIYQ
LGGLGIFLSSKDIQIGRGETIADTAKVLSGYLDGIMIRTFAQEDVEELAKHASIPVINGLTDLYHPCQVLADLQTIKEVK
GKLHGVKLVYIGDGNNMTHSLMLGAAKTGMHMVAVTPDGYTPNKEVLTKAKSIANQHGAIIEWTSDIATAVQEADVIYTD
VWASMGQEEEQQQREKDFENYQVNESLLEKAKTDVIFMHCLPAHRGEEVTAGVIDGKQSVVFQQAENRLHAQKALMTALM
AD
>Mature_321_residues
TTNTQEINFQQQELKGRDFLTLADYTKDEINYLIDLADYLKKQHKDGVIFEPLKGKTLGMIFEKSSTRTRVSFETGIYQL
GGLGIFLSSKDIQIGRGETIADTAKVLSGYLDGIMIRTFAQEDVEELAKHASIPVINGLTDLYHPCQVLADLQTIKEVKG
KLHGVKLVYIGDGNNMTHSLMLGAAKTGMHMVAVTPDGYTPNKEVLTKAKSIANQHGAIIEWTSDIATAVQEADVIYTDV
WASMGQEEEQQQREKDFENYQVNESLLEKAKTDVIFMHCLPAHRGEEVTAGVIDGKQSVVFQQAENRLHAQKALMTALMA
D

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=310, Percent_Identity=47.0967741935484, Blast_Score=284, Evalue=9e-77,
Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=27.7070063694268, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=42.5076452599388, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=41.0179640718563, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI2367364, Length=320, Percent_Identity=30.3125, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI48994908, Length=352, Percent_Identity=24.7159090909091, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI193204318, Length=329, Percent_Identity=28.2674772036474, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322373, Length=309, Percent_Identity=35.5987055016181, Blast_Score=200, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6322331, Length=323, Percent_Identity=28.1733746130031, Blast_Score=106, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24642586, Length=332, Percent_Identity=26.8072289156627, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_OCEIH (Q8ET05)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691381.1
- ProteinModelPortal:   Q8ET05
- SMR:   Q8ET05
- GeneID:   1015729
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0460
- NMPDR:   fig|221109.1.peg.466
- HOGENOM:   HBG579429
- OMA:   FTVAMYQ
- BioCyc:   OIHE221109:OB0460-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 35785; Mature: 35654

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 118-118 BINDING 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNTQEINFQQQELKGRDFLTLADYTKDEINYLIDLADYLKKQHKDGVIFEPLKGKTLG
CCCCCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEE
MIFEKSSTRTRVSFETGIYQLGGLGIFLSSKDIQIGRGETIADTAKVLSGYLDGIMIRTF
EEEECCCCCEEEEECCCCEEECCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHH
AQEDVEELAKHASIPVINGLTDLYHPCQVLADLQTIKEVKGKLHGVKLVYIGDGNNMTHS
HHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHH
LMLGAAKTGMHMVAVTPDGYTPNKEVLTKAKSIANQHGAIIEWTSDIATAVQEADVIYTD
HEEEHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHH
VWASMGQEEEQQQREKDFENYQVNESLLEKAKTDVIFMHCLPAHRGEEVTAGVIDGKQSV
HHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCHHH
VFQQAENRLHAQKALMTALMAD
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTNTQEINFQQQELKGRDFLTLADYTKDEINYLIDLADYLKKQHKDGVIFEPLKGKTLG
CCCCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEE
MIFEKSSTRTRVSFETGIYQLGGLGIFLSSKDIQIGRGETIADTAKVLSGYLDGIMIRTF
EEEECCCCCEEEEECCCCEEECCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHHHH
AQEDVEELAKHASIPVINGLTDLYHPCQVLADLQTIKEVKGKLHGVKLVYIGDGNNMTHS
HHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHH
LMLGAAKTGMHMVAVTPDGYTPNKEVLTKAKSIANQHGAIIEWTSDIATAVQEADVIYTD
HEEEHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHH
VWASMGQEEEQQQREKDFENYQVNESLLEKAKTDVIFMHCLPAHRGEEVTAGVIDGKQSV
HHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCHHH
VFQQAENRLHAQKALMTALMAD
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376