Click here to switch to the map view.

The map label for this gene is nadE

Identifier: 23097842

GI number: 23097842

Start: 410869

End: 411696

Strand: Direct

Name: nadE

Synonym: OB0387

Alternate gene names: 23097842

Gene position: 410869-411696 (Clockwise)

Preceding gene: 23097841

Following gene: 23097843

Centisome position: 11.32

GC content: 35.63

Gene sequence:

>828_bases
ATGAGTGATTTACAAAAAACGATCATTGAAGATTTGCATGTTGAACCTGAAATTGATCCGAAACAAGAAATTCGTACGCG
AGTAGACTTTTTAAAATCATATTTAAAAAAGCATACATTCTCAACAGGATATGTATTAGGTATGTCTGGTGGACAAGACT
CCACTTTGTTATCAAAGCTCACGCAAATCGCAGTAAATGAACTAAACGAGGAAAACTCTACAAATACATATCAGTTTATT
GGTATGAAGTTACCCTATGGTGTTCAAAAAGATGCCGATGATGTGGATGATGCGATTAAGTTTGTTGAACCAAGTAAAGT
ATTGACAGTAAATATTAAAGAATCTGTAGATGCAAGTGTAAAAGCTTTAGATGGAGCAGGCGTGGAAATTAGTGATTTTC
TAAAAGGAAATGAGAAAGCAAGAGAACGCATGAAAGCTCAATATAGTGTGGCAGGAGCATTTAACTGTTTTGTTTTAGGG
ACAGATCATGCAGCAGAAGCTGTAACAGGTTTTTATACAAAACATGGTGATGGCGCTTGTGATTTAGCACCTTTATTTGG
TCTTAATAAACGCCAAGGTAAGCAGATGTTAGTCGCATTAAATTGTCCGGAGCATTTATATAATAAGAAACCTACTGCTG
ATCTGGAAGATGATCGACCAGCTTTACCAGATGAAGAAGCATTGGGAGTTACTTATGAACAAATTGATGAATTCTTAGAA
GGAAAAGAGGTAGCAGAAGACAGTAAGCGAACAATTGAAGGTCATTATTTAAAAACCATGCATAAACGTGAAGGTGAAGT
AACGTTATATGATCAATGGTGGAAATAA

Upstream 100 bases:

>100_bases
TAAGTGAAGCATTATGGAATAATAAGATGAATCATATTGAACGAGTGAAACAAGCAGTAAAAGATAAAATTAATAAACGA
TAAATTAAGGAGGAAAATGC

Downstream 100 bases:

>100_bases
ATTTAGCAAAGAATGAGTTGTTAGAAGCTCATTCTTTTTTCCTGCAGTAAAATAGTATGATTTTGTCTAAAAATGACTAG
ACTTAGAGTGAAAAATGAAG

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSDLQKTIIEDLHVEPEIDPKQEIRTRVDFLKSYLKKHTFSTGYVLGMSGGQDSTLLSKLTQIAVNELNEENSTNTYQFI
GMKLPYGVQKDADDVDDAIKFVEPSKVLTVNIKESVDASVKALDGAGVEISDFLKGNEKARERMKAQYSVAGAFNCFVLG
TDHAAEAVTGFYTKHGDGACDLAPLFGLNKRQGKQMLVALNCPEHLYNKKPTADLEDDRPALPDEEALGVTYEQIDEFLE
GKEVAEDSKRTIEGHYLKTMHKREGEVTLYDQWWK

Sequences:

>Translated_275_residues
MSDLQKTIIEDLHVEPEIDPKQEIRTRVDFLKSYLKKHTFSTGYVLGMSGGQDSTLLSKLTQIAVNELNEENSTNTYQFI
GMKLPYGVQKDADDVDDAIKFVEPSKVLTVNIKESVDASVKALDGAGVEISDFLKGNEKARERMKAQYSVAGAFNCFVLG
TDHAAEAVTGFYTKHGDGACDLAPLFGLNKRQGKQMLVALNCPEHLYNKKPTADLEDDRPALPDEEALGVTYEQIDEFLE
GKEVAEDSKRTIEGHYLKTMHKREGEVTLYDQWWK
>Mature_274_residues
SDLQKTIIEDLHVEPEIDPKQEIRTRVDFLKSYLKKHTFSTGYVLGMSGGQDSTLLSKLTQIAVNELNEENSTNTYQFIG
MKLPYGVQKDADDVDDAIKFVEPSKVLTVNIKESVDASVKALDGAGVEISDFLKGNEKARERMKAQYSVAGAFNCFVLGT
DHAAEAVTGFYTKHGDGACDLAPLFGLNKRQGKQMLVALNCPEHLYNKKPTADLEDDRPALPDEEALGVTYEQIDEFLEG
KEVAEDSKRTIEGHYLKTMHKREGEVTLYDQWWK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=272, Percent_Identity=61.7647058823529, Blast_Score=354, Evalue=3e-99,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_OCEIH (Q8ET75)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691308.1
- ProteinModelPortal:   Q8ET75
- SMR:   Q8ET75
- GeneID:   1015673
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0387
- NMPDR:   fig|221109.1.peg.392
- HOGENOM:   HBG351567
- OMA:   GLNKRRV
- ProtClustDB:   PRK00768
- BioCyc:   OIHE221109:OB0387-MONOMER
- BRENDA:   6.3.1.5
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30788; Mature: 30657

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: ACT_SITE 49-49

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLQKTIIEDLHVEPEIDPKQEIRTRVDFLKSYLKKHTFSTGYVLGMSGGQDSTLLSKL
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHH
TQIAVNELNEENSTNTYQFIGMKLPYGVQKDADDVDDAIKFVEPSKVLTVNIKESVDASV
HHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEHHHCCCHH
KALDGAGVEISDFLKGNEKARERMKAQYSVAGAFNCFVLGTDHAAEAVTGFYTKHGDGAC
HHHCCCCCCHHHHHCCCHHHHHHHHHHHHHCEEEEEEEEECCHHHHHHHHHHHCCCCCCC
DLAPLFGLNKRQGKQMLVALNCPEHLYNKKPTADLEDDRPALPDEEALGVTYEQIDEFLE
CHHHHCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHC
GKEVAEDSKRTIEGHYLKTMHKREGEVTLYDQWWK
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure 
SDLQKTIIEDLHVEPEIDPKQEIRTRVDFLKSYLKKHTFSTGYVLGMSGGQDSTLLSKL
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHH
TQIAVNELNEENSTNTYQFIGMKLPYGVQKDADDVDDAIKFVEPSKVLTVNIKESVDASV
HHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEHHHCCCHH
KALDGAGVEISDFLKGNEKARERMKAQYSVAGAFNCFVLGTDHAAEAVTGFYTKHGDGAC
HHHCCCCCCHHHHHCCCHHHHHHHHHHHHHCEEEEEEEEECCHHHHHHHHHHHCCCCCCC
DLAPLFGLNKRQGKQMLVALNCPEHLYNKKPTADLEDDRPALPDEEALGVTYEQIDEFLE
CHHHHCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHC
GKEVAEDSKRTIEGHYLKTMHKREGEVTLYDQWWK
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376